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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Jurnal Teknologi Industri Pertanian VALENSI BERKALA FISIKA JURNAL KIMIA SAINS DAN APLIKASI Jurnal Penelitian Hasil Hutan The Journal of Pure and Applied Chemistry Research Journal of Engineering and Technological Sciences Jurnal Sains Dan Teknologi Lingkungan Majalah Obat Tradisional Jurnal Sains & Teknologi Lingkungan Jurnal Kimia Riset Jurnal Hasil Penelitian Industri INDONESIAN JOURNAL OF ESSENTIAL OIL Al-Kimia ALCHEMY Jurnal Penelitian Kimia Indonesian Journal of Chemistry Biosaintifika: Journal of Biology & Biology Education Jurnal Purifikasi Chemistry Indonesian Journal of Chemical Research Abdimas Umtas : Jurnal Pengabdian kepada Masyarakat ASEAN Journal on Science and Technology for Development Jurnal Jamu Indonesia Hisbah: Jurnal Bimbingan Konseling dan Dakwah Islam Jurnal Ilmiah Kebidanan Indonesia (Indonesian Midwifery Scientific Journal) Jurnal Penelitian Hasil Hutan Indonesian Journal of Chemical Studies (Indones. J. Chem. Stud.) Milang Journal of Mathematics and Its Applications Analit: Analytical and Environmental Chemistry LIMNOTEK Indonesian Journal of Jamu Jurnal Penelitian Hasil Hutan MILANG Journal of Mathematics and Its Applications Analit: Analytical And Environmental Chemistry
Eti Rohaeti
Department Of Chemistry, Faculty Of Mathematics And Natural Science, IPB University, Bogor|IPB University|Indonesia;Tropical Biopharmaca Research Center, IPB University,Bogor|IPB University|Indonesia
Religion Agriculture, Biological Sciences & Forestry Arts Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Energy Engineering Environmental Science Health Professions Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Other

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Identification of Strobilanthes crispa from Its Related Plant Using Thin-layer Chromatography Fingerprint Analysis Hasanah, Uswatun; Irene, Angelina; Mulyati, Ade Heri; Batubara, Irmanida; Rafi, Mohamad; Rohaeti, Eti
Majalah Obat Tradisional Vol 30, No 2 (2025)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mot.95008

Abstract

Strobilanthes crispa known as keji beling in Indonesia, belong to the Acanthaceae family. Thin layer chromatography (TLC) fingerprint pattern analysis is an excellent technique for evaluating and controlling the quality of raw materials containing Strobilanthes crispa. TLC fingerprints can be used to analyze the fingerprint profiles of medicinal plants for identification, authentication, and discrimination from related plants. This study aimed to develop a TLC fingerprint analysis method to identify S. crispa for quality control. Eleven bands were effectively separated using a silica gel 60 F254 TLC plate with a mobile phase of dichloromethane, ethyl acetate, and chloroform at 16:3.2:0.8. The derivatization reagent used to detect the separated bands was 10% sulfuric acid at a wavelength of 366 nm. Validation of the TLC fingerprint analysis, which evaluated the robustness, stability, specificity, and precision, met these requirements. S. crispa can be distinguished by its TLC fingerprint from sirih hutan (Piper aduncum), a related plant with similar leaf morphology. This approach could be used to identify and authenticate of S. crispa from P. aduncum.
PENGGUNAAN METODE HOMOTOPI PADE’ UNTUK MENYELESAIKAN MASALAH LOTKA-VOLTERRA ROHAETI, E.; JAHARUDDIN, J.; KUSNANTO, A.
MILANG Journal of Mathematics and Its Applications Vol. 11 No. 1 (2012): Journal of Mathematics and Its Applications
Publisher : School of Data Science, Mathematics and Informatics, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jmap.11.1.31-40

Abstract

Model Lotka- Volterra merupakan model interaksi antar spesies mangsa dan spesies pemangsa pada sebuah lingkungan dan dinyatakan dalam bentuk persamaan taklinear. Persamaan Lotka-Volterra diselesaikan dengan metode homotopi Pade'. Metode hemotopi Pade' merupakan pengembangan dari metode homotopi. Dalam hal ini, penyelesaian dengan menggunakan metode homotopi Pade' diperoleh dengan menggunakan penyelesaian dengan menggunakan metode homotopi. Metode homotopi Pade’ lebih cepat mencapai kekonvergenan dibandingkan dengan metode homotopi. Dengan metode homotopi Pade' diberikan suatu interpretasi fisis untuk kasus mangsa lebih banyak dari pemangsa.
Spektrofotometer Quali-Vis dan Kemometrika untuk Klasifikasi Kualitas Daun Wungu (Graptophyllum pictum) Putri, Silvi Agusri; Heryanto, Rudi; Rohaeti, Eti
Jurnal Jamu Indonesia Vol. 3 No. 3 (2018): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v3i3.66

Abstract

Spektrofotometer Quali-Vis digunakan untuk mengklasifikasikan 3 sampel daun wungu dengan usia panen berbeda. Penelitian ini bertujuan mengevaluasi model klasifikasi menggunakan spektrofotometer Quali-Vis secara kualitatif. Model dikembangkan dengan akuisisi data menggunakan principle component analysis (PCA) dan partial least square-discriminant analysis (PLS-DA). Karakteristik awal sampel daun wungu ditentukan dengan analisis sidik jari kromatografi lapis tipis (KLT) yang menampilkan profil yang berbeda pada masing-masing usia panen. Perbedaan ini menjadi dasar klasifikasi untuk model PLS-DA yang terdiri atas model usia 1 bulan, 2 bulan, dan 3 bulan, yang menghasilkan rerata R2pred = 0.7794 dan root mean square error predict (RMSEP) = 0.2180. Model divalidasi dengan menentukan nilai parameter sensitivitas, spesifitas, presisi, dan akurasi yang menghasilkan nilai rerata masing-masing 0.95, 0.99, 0.98, dan 0.98 kesesuaian prediksi. Nilai-nilai ini menunjukkan bahwa model yang dikembangkan memiliki kemampuan klasifikasi yang cukup baik.
Analisis Sidik Jari Kromatografi Lapis Tipis Rimpang Temu Mangga (Curcuma mangga) Syafi’i, Makmum; Rohaeti, Eti; Wahyuni, Wulan Tri; Rafi, Mohamad; Septaningsih, Dewi Anggraini
Jurnal Jamu Indonesia Vol. 3 No. 3 (2018): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v3i3.68

Abstract

Profil sidik jari kromatografi lapis tipis (KLT) dapat dimanfaatkan untuk kendali mutu bahan baku maupun produk tumbuhan obat. Penelitian ini bertujuan mengembangkan metode analisis sidik jari rimpang temu mangga (Curcuma mangga) untuk kendali mutunya. Rimpang kering temu mangga diekstraksi menggunakan metanol dan dianalisis sidik jari KLT. Kloroform:etil asetat (8.5:1.5) merupakan fase gerak optimum yang memisahkan 11 pita dari rimpang temu mangga dengan keterpisahan yang baik dan memiliki pita khas biru tua (Rf 0.36). Demetoksikurkumin terdeteksi pada rimpang temu mangga dengan warna jingga kecoklatan pada UV 366 nm (Rf 0.48) setelah diderivatisasi dengan pereaksi asam sulfat 10%. Pengujian spesifitas terhadap rimpang lain dalam satu genus, rimpang temu mangga menunjukan pola yang berbeda. Validasi metode analisis sidik jari KLT memenuhi kriteria keberterimaan, sehingga metode ini dapat digunakan untuk kendali mutu rimpang temu mangga.
Antidiabetic Activity of Manonjaya Snakefruit Skin Extract with Zebra Fish (Danio rerio) as Animal Model Eti Rohaeti; Dea Nurafifah; Irmanida Batubara
Jurnal Jamu Indonesia Vol. 7 No. 3 (2022): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v7i3.201

Abstract

This research aimed to determine the active fraction and profile of active compounds in Manonjaya sSnakefruit skin extract that can be used as antidiabetic and the antidiabetic activity using zebra fish. Manonjaya sSnakefruit skin was extracted by maceration using 70% ethanol and then fractionated using n-hexane, ethyl acetate and methanol. The ethyl acetate fraction showed the highest yield and total phenolic content as well as the strongest α-glucosidase inhibition with an inhibition percentage of 64.43%. The ethyl acetate fraction provided the most active spot as an α-glucosidase inhibitor. The profile of active compounds that act as antidiabetic compound is flavonoid from glycosylflavone and flavone groups. Antidiabetic activity using zebra fish was indicates that Manonjaya Snakefruit skin extract from ethanol extract and ethyl acetate fraction can decrease blood sugar level of zebra fish after induced with alloxan. The crude extract group had 54.51% lower sugar content than the induction group and 41.70% lower than the negative control. Meanwhile, the ethyl acetate fraction group had 65.10% lower sugar content than the induction group and 55.27% lower than the negative control.
Fingerprints, Antioxidant Activity, and Anti-Tyrosinase of Fungus Comb Extract Dewi, Trisni Untari; Diposani, Diva Putri; Rohaeti, Eti; Nandika, Dodi; Karlinasari, Lina; Arinana, Arinana; Santoso, Djoko; Witasari, Lucia Dhiantika; Rachmayanti, Yanti; Batubara, Irmanida
Jurnal Jamu Indonesia Vol. 8 No. 3 (2023): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v8i3.346

Abstract

Fungus comb is a structure made by termite colony Macrotermitinae with lots of potential because it contains secondary metabolites such as steroids, terpenoids, and saponins and compounds containing antioxidant and anti-tyrosinase properties. This study aimed to determine fungus comb extracts' fingerprint pattern, antioxidant capacity, and anti-tyrosinase activity. Thin layer chromatography (TLC) was used to determine fingerprint patterns. The resulting TLC pattern was processed with ImageJ and converted into a densitogram. The fungus comb was extracted with n-hexane and ethyl acetate. TLC results showed that fungus comb extracts contain steroids and terpenoids and have antioxidant properties. The antioxidant capacity assay was carried out with 2,2-diphenyl-1-picrylhydrazyl reagent. N-hexane extract had an antioxidant capacity value of 9.07 mg ascorbic acid equivalent (AAE)/g extract, while ethyl acetate extract had 6.04 mg AAE/g extract. Anti-tyrosinase activity assay was carried out with L-tyrosine and L-3,4-dihydroxyphenylalanine (L-DOPA) as substrates. N-hexane extract had a better anti-tyrosinase activity with IC50 of 1804 µg/mL for L-tyrosine and 6784 µg/mL for L-DOPA.
Development of a Low-Cost Reflectance Visible Spectrophotometer with Chemometrics for Curcuma xanthorrhiza Roxb. Quality Control Heryanto, Rudi; Iswantini, Dyah; Rohaeti, Eti; Rafi, Mohamad; Rahma, Novia; Hafshah, Nurul; Mardiana, Eni
Jurnal Kimia Valensi Jurnal Kimia VALENSI, Volume 10, No. 2, November 2024
Publisher : Department of Chemistry, Faculty of Science and Technology Syarif Hidayatullah Jakarta State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/jkv.v10i2.40351

Abstract

A simple, portable visible reflectance spectrophotometer was developed for classifying the quality of Curcuma xanthorrhiza Roxb. The device was assembled using consumer electronic components: an LED as the light source, a DVD optical layer as the diffraction grating, and a webcam as the detector, with web-based software (Spectral Workbench) as the signal processor. The spectrophotometer's performance was evaluated using standard Sudan III samples and powdered C. xanthorrhiza samples from two quality classes (A and B). Spectral data were analysed using principal component analysis (PCA) and partial least squares (PLS) regression.  PCA successfully grouped Sudan III samples by concentration and classified C. xanthorrhiza by curcuminoid content. A PLS regression model was developed for quantifying Sudan III, yielding R² values of 0.9909 for both calibration and cross-validation, with RMSEs of 0.3821% and 0.4152%, respectively. The results closely matched those from a commercial spectrophotometer. A PLS-discriminant analysis (PLS-DA) classification model for C. xanthorrhiza was also developed, achieving sensitivity and specificity values of 1. Additionally, semi-quantitative parameters such as decision limit (26.15% B), detection capability (41.06% B), and unreliability range (26.15–41.06% B) were calculated. The classification model showed strong sensitivity, specificity, and detection capabilities within acceptable limits. These findings suggest that this low-cost reflectance spectrophotometer, combined with chemometric methods, holds promise as a practical tool for the quality control of raw herbal materials.
KARAKTERISTIK KARBON AKTIF DARI KULIT BUAH MALAPARI (Pongamia pinnata L. Pierre) Ibrahim; Djeni Hendra; Nur Adi Saputra; Eti Rohaeti
Jurnal Penelitian Hasil Hutan Vol. 40 No. 1 (2022): Jurnal Penelitian Hasil Hutan
Publisher : BRIN Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jphh.2022.40.1.1-6

Abstract

Vegetable oil production of malapari (Pongamia pinnata L. Pierre) fruit remains peels that haven't been utilized. Malaparifruit peel is potential for activated carbon. The purpose of this research is to use malapari fruit peel waste as raw material forproducing activated charcoal. Samples were carbonized at temperature of 400°C and continued activation with phosphoric acid2% and physical activation at temperature of 750°C by flowing steam for 60 minutes. The process resulted in the Optimumcondition. It yields 54% charcoal recovery, moisture content of 8.6%, volatile matter of 11.85%, ash content of 24.73%, fixedcarbon of 63.42%, benzene adsorptivity of 10.15%, metilene blue adsorptivity of 93.89 mg/g, iodine adsorptivity of 648.62mg/g, and specific surface area of 348.11 m2/g. Activated carbon in this study is below Indonesian standards, which is influencedby the activation retention time.
AKTIVITAS ANTIBAKTERI DAN TOKSISITAS MINYAK SERAI DAPUR (Cymbopogon citratus) DENGAN PERLAKUAN PEMEKATAN PADA SUHU BERBEDA Febriani, Reni; Rohaeti, Eti; Wahyuni, Wulan Tri
Analit : Analytical and Environmental Chemistry Vol. 6, No. 02 October (2021) Analit : Analytical and Environmental Chemistry
Publisher : Jurusan Kimia FMIPA Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/aec.v6i2.2021.p168-179

Abstract

Minyak atsiri serai (Cymbopogon citratus) dapur telah dipergunakan secara luas di berbagai industri, diantaranya sebagai agen antibakteri. Pada penelitian ini dievaluasi aktivitas antibakteri dan toksisitas minyak serai dapur yang dipekatkan pada suhu berbeda. Toksisitas dievaluasi melalui uji brine shrimp lethality test (BSLT), sementara uji antibakteri dilakukan terhadap bakteri Staphylococcus aureus dan S. epidermidis dengan metode difusi dan dilusi. Komponen kimia yang terdapat dalam minyak serai dianalisis menggunakan kromatografi gas spektrometri massa (GC-MS). Hasil yang diperoleh menunjukkan berdasarkan uji BSLT diperoleh nilai lethal concentration 50 (LC50) sampel minyak serai dapur semua perlakuan < 1000 ppm dengan LC50 terendah sebesar 16,53 ± 2,82 ppm. Nilai konsentrasi hambat minimun (KHM) dan konsentrasi bunuh minimum (KBM) minyak serai dapur terhadap S. aureus umumnya lebih rendah dibanding terhadap S. epidermidis. Aktivitas antibakteri minyak serai dapur terhadap S. aureus terkategori sangat kuat dengan nilai KHM dan KBM terendah ditunjukkan oleh minyak serai tanpa perlakuan pemekatan yaitu sebesar 1250 dan 2500 ppm terhadap S. aureus. Komposisi senyawa kimia utama dalam minyak serai dapur berdasarkan analisis GC-MS ialah sitral, neral dan β-myrcenehttp://dx.doi.org/10.23960/aec.v6.i2.2021.p168-179
MODIFIKASI SCREEN PRINTED CARBON ELECTRODE DENGAN POLIMER TERCETAK MOLEKUL (MIP) DAN APLIKASINYA UNTUK DETEKSI GLUTATION Rohaeti, Eti; Wahyuni, Wulan Tri; Hakim, Moh Arif Rohman Nur
Analit : Analytical and Environmental Chemistry Vol. 3, No. 01 April (2018) Analit : Analytical and Environmental Chemistry
Publisher : Jurusan Kimia FMIPA Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/aec.v3i1.2018.p

Abstract

Penelitian ini bertujuan memodifikasi screen printed carbon electrode (SPCE)  dengan polimer tercetak molekul (MIP) dan mengaplikasikannya untuk deteksi glutation tereduksi (GSH) secara tidak langsung dengan mediator bromin menggunakan teknik voltammetri siklik. SPCE dimodifikasi dengan MIP polipirola (MIPpy) melalui teknik elektrodeposisi. Pengukuran GSH dilakukan dalam elektrolit NaClO4 0.2 M yang mengandung 1 mM NaBr. Hasil yang diperoleh menunjukkan SPCE-MIPpy telah berhasil diperoleh melalui teknik elektrodeposisi. Pada pengukuran diperoleh puncak oksidasi bromida pada potensial ±0.63 V vs. Ag/AgCl. Puncak ini berkorelasi linear dengan konsentrasi GSH dalam larutan sehingga dapat digunakan sebagai dasar penentuan GSH. Polipirola meningkatkan konduktivitas listrik elektrode sehingga dapat meningkatkan puncak arus oksidasi GSH. Pengukuran glutation dengan SPCE-MIPpy menunjukkan limit deteksi dan limit kuantitasi yang lebih baik dibandingkan SPCE, yaitu sebesar 7.6626 µM dan 25.4092 µM. Keberadaan interference glukosa tidak mengganggu pengukuran glutation. Kinerja SPCE-MIPpy dalam pengukuran GSH disimpulkan lebih optimum dibandingkan SPCE.  DOI: http://dx.doi.org/10.23960/aec.v3.i1.2018.p47-60 
Co-Authors A. KUSNANTO Abdul Rohman Achmad Fauzi Achmad Fauzi Ade Heri Mulyati, Ade Heri Aditya Kurniawan Aditya Kurniawan Agustina, Rofiqoh Inayati Aini, Nazwa Aisyah Akhmad Aghzath, Ananjaya Ali Mahmoud Muddathir Ali Mahmoud Muddathir Ali Miftahudin Anak Agung Gede Sugianthara Antonio Kautsar Arinana Arinana Arnold Patogi Siahaan Arnold Patogi Siahaan Aryo Tedjo Asih Gayatri Astrid Novita, Astrid Bambang Prajogo Eko Wardoyo Budi Riza Putra Budi Riza Putra Budi Riza Putra Budi Santoso, Arianto Casnan Casnan Casnan, Casnan Citra Ajeng Palupi Dea Nurafifah Dea Nurafifah Deden Saprudin Derry Permana Dewi Anggraeni Septaningsih Diposani, Diva Putri Djeni Hendra Djeni Hendra Djoko Santoso Dodi Nandika Dudy Imanuddin Effendi Dyah Iswantini Eni Sumarni Erliza Noor Erniati Fadila Karunina Febriani, Elsa Fendy Folkes Laumal Gunawan, Fransisca Hadjomidjojo, Hartrisari Hafshah, Nurul Hartrisari Hadjomidjojo Hartrisari Hardjomidjojo Herry Suhardiyanto Ibrahim Ibrahim Ibrahim Ida Ayu Putu Sri Widnyani Irene, Angelina Irma Isnafia Arief Irmanida Batubara Irzaman, Irzaman Jaharuddin Jaharuddin, Jaharuddin Karomah, Alfi Hudatul Khoirotul Muzayanah KUSNANTO, A. Latifah K Darusman Latifah K Darusman Latifah K. Darusman Latifah K. Darusman Latifah Kosim Darusman Latifah Kosim Darusman Lina Karlinasari Linda Trivana Linda Trivana Linda Trivana, Linda Lucia Dhiantika Witasari, Lucia Dhiantika M. Rafi Madya, Muhammad Miftahul Makmum Syafi’i Manggar Arum Aristri Mardiana, Eni Moh Arif Rohman Nur Hakim Moh Nur Hakim Mohamad Rafi Mohamad Solahudin Muddathir, Ali Mahmoud Muhammad Bachri Amran Munandar, Rafly N. G. Pamungkas Novia Rahma Nur Adi Saputra Nur Adi Saputra Nurdiani Nurdiani, Nurdiani Nurhajawarsi Nurhajawarsi Nursifa Mawadah Purnawan Purnawan Purnawan Purnawan Putri, Silvi Agusri Rachmayanti, Yanti Rahadyanoto Trimulia Rahmawati, Heni Rahmawati, Wahyu Riski Ramadhanti, Desi Reni Febriani Reni Febriani, Reni Rinaldi Idroes Rudi Heryanto Santoso, Arianto Budi Satriah, Lilis - Septaningsih, Dewi Anggraini Shadila Fira Asoka Siahaan, Arnold Patogi Silvia, Riva Siti Aisyah Slamet Widodo Sri Sugiarti Supriyanto Syafi’i, Makmum Syifa Muflihah Tamida Elinda Taopik Ridwan Trisni Untari Dewi Ummu Salma Al Azizah Uswatun Hasanah Utami Dyah Syafitri Wulan Tri Wahyuni Yudiwanti Yuspian Nur Zaenal Abidin