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KHOMAINI HASAN
Faculty Of Medicine, Universitas Jenderal Achmad Yani, Cimahi, Indonesia
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Dentistry Earth & Planetary Sciences Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Public Health Social Sciences Veterinary Other

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Journal : jurnal sains indonesia

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Pengaruh Suhu pada Ekspresi Pretrombin-2 Manusia Rekombinan di Escherichia coli ER2566 Silaban, Saronom; Simorangkir, Murniaty; Maksum, Iman Permana; Subroto, Toto; Hasan, Khomaini
Jurnal Sains Indonesia Vol 42, No 1 (2018): Jurnal Sains Indonesia
Publisher : Universitas Negeri Medan

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Abstract

Human recombinant prethrombin-2 (rhPT-2) was expressed in Escherichia coli. Previous studies reported that the expression of rhPT-2 in E. coli tends to formed inclusion body. This study aims to determine the effect of temperature on the expression of rhPT-2 in E. coli ER2566. This study begins with the isolation of recombinant plasmids, competent cell preparation and transformation of competent cells of E. coli ER2566 strain, and rhPT-2 gene expression with various induction temperatures 12, 18, 22 and 30°C. Characterization results showed that the induction temperature of 22°C was the optimum temperature of rhPT-2 gene expression as a soluble fraction in E. coli ER2566 with IPTG concentration 0.1 mM. These results prove that the temperature affects the expression of rhPT-2 in E. coli ER2566.
Pengaruh Suhu pada Ekspresi Pretrombin-2 Manusia Rekombinan di Escherichia coli ER2566 Silaban, Saronom; Simorangkir, Murniaty; Maksum, Iman Permana; Hasan, Khomaini
Jurnal Sains Indonesia Vol 42, No 2 (2018): Edisi Juli - Desember
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jsi.v42i2.12245

Abstract

Human recombinant prethrombin-2 (rhPT-2) was expressed in Escherichia coli. Previous studies reported that the expression of rhPT-2 in E. coli tends to formed inclusion body. This study aims to determine the effect of temperature on the expression of rhPT-2 in E. coli ER2566. This study begins with the isolation of recombinant plasmids, competent cell preparation and transformation of competent cells of E. coli ER2566 strain, and rhPT-2 gene expression with various induction temperatures 12, 18, 22 and 30°C. Characterization results showed that the induction temperature of 22°C was the optimum temperature of rhPT-2 gene expression as a soluble fraction in E. coli ER2566 with IPTG concentration 0.1 mM. These results prove that the temperature affects the expression of rhPT-2 in E. coli ER2566. [EFFECT OF TEMPERATURE ON RECOMBINANT HUMAN PRETROMBIN-2 EXPRESSION IN ESCHERICHIA COLI ER2566] (J. Sains Indon., 42(2): 25-30, 2018)Keywords:Induction Temperature, Soluble Fraction, Prethrombin-2, E. coli ER2566
Co-Authors Achmad, Nurjannah Akmal Dhaffa Ulhaq Amallia Ardila Putri Andreanus Andaja Soemardji Andreas A Soemardji Andrian R, Andri Ania Kurniawati Purwa Dewi Arsyad Parama Santosa Audrey, Gabriella Budiantoro, Ogi Cicilia Liempapas D Agus Yusuf Wildan Daswara Djajasasmita Desi Veronika Pardosi DESSY NATALIA Dewi R Handayani Dian Anggraeny Eka Noneng Nawangsih Elly Noer Rochmah, Elly Noer Endry Septiadi Endry Septiadi et al Evi Sovia FERNITA PUSPASARI Gabriella Audrey Gabriella, Janifer Handayani, Dewi Ratih I Gede Eka Perdana Putra, I Gede Eka Perdana Iis I Rakhmat Iis Inayati Rahmat Ilma Fiddiyanti Iman Permana Maksum Inayati, Iis Indarti Trimurtini Indriyana, Sri Quintina IS HELIANTI Janifer Gabriella Liempapas, Cicilia Luvina, Petrisia Maria Ulfah Meilinah Hidayat MEILINAH HIDAYAT Mohammad Yusuf Muhammad Fadhil Z, Rusdi Noor Rosa (p:43-50) Murniaty Simorangkir Mutiara, Dinar N Ferdianti, Fine NIKNIK NURHAYATI Ogi Budiantoro OGI BUDIANTORO P Y Paryati, Sayu Pertiwi, Wulan Petrisia Luvina Purba Upay Ratunanda, Susanti Riezki Amalia Rini Roslaeni Sabbathini, Gabriela Christy Safari, Ardhi Ryanto SARONOM SILABAN Saronom Silaban Saronom Silaban Sayu Putu Yuni Paryati Sayu Putu Yuni Paryati Septiadi, Endry Shabarni Gaffar Sigit Sasongko Sijani Prahastuti Sijani Prahastuti Sijani Prahastuti Siska Telly Pratiwi Soemardji, Andreas A Soetijoso Soemitro SOETIJOSO SOEMITRO Soetijoso Soemitro Soetijoso Soemitro Soetijoso Soemitro Sutan Bagas Prakasa SUTARYA ENUS Sutarya Enus Sutarya Enus Sutarya Enus Toto Subroto Upay, Purba WANGSA TIRTA ISMAYA Welly Ratwita Wulan Pertiwi Wulandari, Sri Rezeki Yudith Y Kusmala Yudith Yunia K Yulistio Hanif