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All Journal HAYATI Journal of Biosciences Biotika: Jurnal Ilmiah Biologi Indonesian Journal of Applied Sciences Bioscience Biogenesis: Jurnal Ilmiah Biologi Buletin Penelitian Tanaman Rempah dan Obat Quagga JURNAL ISTEK Jurnal Biodjati Jurnal BIOEDUIN : Program Studi Pendidikan Biologi Buletin Penelitian Tanaman Rempah dan Obat Jurnal Agrinika : Jurnal Agroteknologi dan Agribisnis Riset Informasi Kesehatan
Nia Rossiana
Departemen Biologi Fakultas Matematika Dan Ilmu Pengetahuan Alam Universitas Padjadjaran
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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Metabolites Profiling of Penicillium citrinum Recovered from Endophytic of Ramie (Boehmeria nivea) as a Potential Biocontrol Against Pathogenic Fungi Kartika Sari; Vira Kusuma Dewi; Asri Peni Wulandari; Nia Rossiana; Tati Herlina; Fitri Widiantini
HAYATI Journal of Biosciences Vol. 30 No. 2 (2023): March 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.2.246-255

Abstract

Endophytes are a potent source of bioactive compounds that mimic plant-based metabolites. Fungi Fusarium spp. and Clonostachys rosea have been identified as pathogenic microorganisms in ramie (Boehmeria nivea). The antifungal test was carried out using the diffusion method and the MIC50 and Minimum Fungicidal Concentration (MFC) values using the tested microbial pathogens were Fusarium solani isolate 3248941, F. solani isolate Colpat-359, F. oxysporum isolate N-61-2, dan Clonostachys rosea strain B3042. Identification of secondary metabolites of the extract was carried out using GC-MS. The chromatogram of GC-MS analysis of this ethyl acetate extract (EA) showed seven dominant chemical compounds with various biological activities. Ethyl asetate extract of P. citrinum showed inhibition zone ranged from 19.10–22.07 mm with strong-very strong category at 200 mg ml-1 concentration against the tested microbial pathogens. Results revealed that the significant MIC values were observed against F. solani isolate 3248941 and Clonostachys rosea strain B3042 by less than 6.3  mg ml-1 and against F. solani isolate Colpat-359 and F. oxysporum isolate N-61-2 with 12.5 mg ml-1 and 25 mg ml-1 respectively. Minimum Fungicidal concentrations (MFC) for EA of P. citrinum were also reported against F. solani isolate 3248941 and C. rosea strain B3042, by 12.5 mg ml-1, whilst Fusarium solani isolate Colpat-359 by 25 mg ml-1 and Fusarium oxysporum isolate N-61-2 ,by 50 mg ml-1 respectively. Results of this study showed that P. citrinum, endophytic fungi of ramie, could be a promising source of compounds for antifungal agents.
UJI KUALITAS UDANG BEKU SECARA BAKTERIOLOGIS DARI UNIT PENGOLAHAN IKAN WILAYAH CIREBON, JAWA BARAT Syaputri, Yolani; Nurkhotimah, Retna; Gunawan, Eko Hendri; Amdhika, Ries; Rahayuningsih, Sri Rejeki; Rossiana, Nia
BIOTIKA Jurnal Ilmiah Biologi Vol 21, No 1 (2023): BIOTIKA JUNI 2023
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/biotika.v21i1.41589

Abstract

Udang beku merupakan komoditas penting dari sektor perikanan Indonesia. Nilai ekspor udang beku dari tahun ke tahun selalu mengalami peningkatan khususnya dari Unit Pengolahan Ikan (UPI) Cirebon Jawa Barat. Angka Lempeng Total (ALT) serta bakteri patogen seperti Salmonella spp. dan Escherichia coli merupakan parameter bakteriologis yang dapat digunakan sebagai indikator adanya kontaminasi dan kualitas yang kurang baik pada udang. Penelitian ini bertujuan untuk menguji angka lempeng total serta mendeteksi adanya cemaran bakteri Salmonella spp. dan E.coli pada sampel udang beku. Penelitian bersifat deskriptif eksploratif dengan melakukan penelitian di Laboratorium Stasiun Karantina Ikan Pengendalian Mutu dan Keamanan Hasil Perikanan Cirebon. Berdasarkan standar baku mutu udang beku SNI 2705 : 2020, jumlah angka lempeng total pada udang beku harus dibawah 5.0 x 105 CFU/g serta harus negatif dari bakteri Salmonella spp. dan E. coli. Berdasarkan hasil pengujian keempat sampel udang beku dari UPI wilayah Cirebon Jawa Barat yaitu pada ke empat titik sampel UPI A, B, C, dan D, jumlah angka lempeng total keempat sampel memenuhi syarat standar baku mutu udang beku. Nilai paling tinggi berasal dari UPI C dan yang paling rendah berasal dari UPI D. Hasil analisis bakteri patogen, keempat sampel udang beku negatif bakteri Salmonella spp. dan E. coli.
Bioremediation using microbial fuel cell for polluted marine sediment Pribadi, Tri Dewi Kusumaningrum; Rosada, Keukeu Kaniawati; Ihsan, Yudi Nurul; Rossiana, Nia
Biogenesis: Jurnal Ilmiah Biologi Vol 11 No 2 (2023)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v11i2.38765

Abstract

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Exploration of pathogenic bacteria from lettuce (Lactuca sativa) in a Canteen at Universitas Padjadjaran Chan, Rosamond; Syaputri, Yolani; Syamsuri, Rizky Riscahya Pratama; Rossiana, Nia
Riset Informasi Kesehatan Vol 13 No 2 (2024): Riset Informasi Kesehatan
Publisher : Sekolah Tinggi Ilmu Kesehatan Harapan Ibu Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30644/rik.v13i2.872

Abstract

Background: Lettuce (Lactuca sativa) is commonly consumed raw as a salad, making it highly susceptible to contamination by pathogenic bacteria. This study aims to identify the presence of pathogenic bacteria on raw lettuce to offer an alternative method for handling these pathogens and improving public health. Method: In this research, lettuce samples were bacteriologically tested to identify the presence of pathogenic bacteria. Isolation was performed using selective media, Salmonella-Shigella (SS) agar and Eosin Methylene Blue (EMB) agar, to obtain pathogenic bacteria. Identification was conducted through macroscopic and microscopic examinations, as well as biochemical tests, including indole, Methyl Red, Voges-Proskauer, citrate, motility, and catalase tests. Results: The results of the study revealed the presence of four bacterial isolates: SS 1, SS 2, EMB 1, and EMB 2. Based on the study and identification using "Microbiology: A Laboratory Manual 12th Edition," isolates SS 1, EMB 1, and EMB 2 were identified as Alcaligenes sp., while isolate SS 2 was identified as Proteus sp. Conclusion: These findings provide essential knowledge for managing pathogenic bacterial contamination on lettuce, potentially enhancing food safety and public health.
Co-Authors Agus Widana agus widana Almira Salma Nabilah Amdhika, Ries Annie Satriani Achwani Asri Peni Wulandari Asri Peni Wulandari Atasya Yasmine Fakhira Bambang Priadie Betty Mayawatie Marzuki, Betty Mayawatie Chan, Rosamond Dadan Sumiarsa Desak Made Malini Diana Indah Permatasari Dono Wahyuno Dwi Astuti Aprilia Eko Hendri Gunawan, Eko Hendri Febri Doni Felicia Fiandisty Fitri Widiantini Hikmat Kasmara Ida Indrawati Ida Indrawati Ida Indrawati Ida Indrawati Ina Darliana Johan Iskandar Johan Iskandar Kartika Sari Keukeu Kaniawati Rosada Lestari Rahayu Mia Miranti Mia Miranti Muhammad Faizal Fathurrohim Nana Media Nindira Tiara Putri Nindya Ayuni Lestari Nisa Amalia Nurkhotimah, Retna Nurliani Bermawie Putri Kumala Dewi R. Ninda Naufal Utami Ratu Safitri Risa Widyasti Rizky Riscahya Pratama Syamsuri Sri Rejeki Rahayuningsih Sulistya Ika Akbari Syamsuri, Rizky Riscahya Pratama Syaputri, Yolani Tati Herlina Tia Setiawati Titin Supriatun Titin Supriatun Tri Dewi Kusumaningrum Pribadi Tri Rahayu Hidayat Vira Kusuma Dewi Wida Widyawati Yudi Nurul Ihsan