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All Journal SAINS MEDIKA : JURNAL KEDOKTERAN DAN KESEHATAN Jurnal Analis Kesehatan Business Management Analysis Journal (BMAJ) Cendekia Journal of Pharmacy Jurnal Keperawatan Priority JURNAL ANALIS LABORATORIUM MEDIK Jurnal Kesehatan Masyarakat Celebes Jaringan Laboratorium Medis Jurnal Peduli Masyarakat Jurnal Health Sains Jurnal Kesehatan Tambusai Muria Jurnal Layanan Masyarakat Prosiding University Research Colloquium Termometer: Jurnal Ilmiah Ilmu Kesehatan Dan Kedokteran Jurnal Medika: Medika Jurnal Abdimas Indonesia Jurnal Medika Indonesia
Arief Adi Saputro
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Journal : Jaringan Laboratorium Medis

 1 
Evaluasi Akurasi dan Presisi Alat Hematology Analyzer Mindray BC-5380 Mode Predilute Terhadap Kadar Hemoglobin pada Darah Kapiler Apriliani, Dwi; Mundriyastutik, Yayuk; Saputro, Arief Adi
Jaringan Laboratorium Medis Vol 7, No 1 (2025): May 2025
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v7i1.12778

Abstract

Technological advances affect the automation of laboratory equipment, one of which is haemoglobin examination with a haematology analyzer that has several modes of examination to improve accuracy and precision. The haemoglobin test data at RSU Fastabiq Sehat PKU Muhammadiyah Pati reached 25,365 tests. Predilute mode on the Mindray BC-5380 haematology analyser is rarely used, only about 2%, very much different from the use of Whole Blood (WB) mode which reaches a percentage of 98%. This study aims to evaluate the accuracy and precision of the Hematology Analyzer Mindray BC-5380 in Predilute mode for measuring haemoglobin levels using capillary blood at RSU Fastabiq Sehat PKU Muhammadiyah Pati. The methodology used in this study was descriptive quantitative with a cross-sectional approach. Data was obtained through measurement of haemoglobin using the device, which was then analysed to assess accuracy and precision. The results showed a bias value of 2.58%, indicating good accuracy. The coefficient of variation (CV) value of 5.52% exceeded the limit set by the manufacturer, indicating poor precision. Further evaluation using the Levey-Jenning graph and Westgard's rule indicated a 12s deviation on the fifth day, indicating the possibility of systematic or random errors. The Mindray BC-5380 predilute mode haematology analyser had good accuracy of haemoglobin levels in capillary blood, but poor precision compared to the manufacturer's values.
Pengaruh Stabilitas Sampel Darah EDTA Terhadap Pemeriksaan Hemoglobin dan Hematokrit pada Karyawan Puskesmas Kedung II Metode Otomatis Anjani, Anisa Ayu; Hartinah, Dewi; Kurnia, Shinta Dwi; Rusidah, Yunita; Saputro, Arief Adi
Jaringan Laboratorium Medis Vol 7, No 1 (2025): May 2025
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v7i1.12872

Abstract

This study aims to determine the effect of EDTA blood sample stability on hemoglobin (Hb) and hematocrit (Ht) examination results using an automatic method. EDTA blood samples from 38 employees of Kedung II Public Health Center were examined immediately (0 hours), after a 1-hour delay at room temperature (17–210C), and after a 1-hour delay in a refrigerator (2–80C). The results were analyzed using a Two-Way ANOVA test. The study showed no significant differences in hemoglobin and hematocrit levels between the three storage conditions (ρ 0.05). The average hemoglobin levels were 12.834 g/dL at 0 hours, 12.797 g/dL after 1 hour at room temperature, and 12.829 g/dL after 1 hour in the refrigerator. The average hematocrit levels were 38.484% at 0 hours, 38.321% after 1 hour at room temperature, and 38.434% after 1 hour in the refrigerator. The conclusion of this study is that storing EDTA blood samples for 1 hour, whether at room temperature or in the refrigerator, does not significantly affect hemoglobin and hematocrit examination results.
Evaluasi Akurasi dan Presisi Alat Hematology Analyzer Mindray BC-5380 Mode Predilute Terhadap Kadar Hemoglobin pada Darah Kapiler Apriliani, Dwi; Mundriyastutik, Yayuk; Saputro, Arief Adi
Jaringan Laboratorium Medis Vol. 7 No. 1 (2025): May 2025
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v7i1.12778

Abstract

Technological advances affect the automation of laboratory equipment, one of which is haemoglobin examination with a haematology analyzer that has several modes of examination to improve accuracy and precision. The haemoglobin test data at RSU Fastabiq Sehat PKU Muhammadiyah Pati reached 25,365 tests. Predilute mode on the Mindray BC-5380 haematology analyser is rarely used, only about 2%, very much different from the use of Whole Blood (WB) mode which reaches a percentage of 98%. This study aims to evaluate the accuracy and precision of the Hematology Analyzer Mindray BC-5380 in Predilute mode for measuring haemoglobin levels using capillary blood at RSU Fastabiq Sehat PKU Muhammadiyah Pati. The methodology used in this study was descriptive quantitative with a cross-sectional approach. Data was obtained through measurement of haemoglobin using the device, which was then analysed to assess accuracy and precision. The results showed a bias value of 2.58%, indicating good accuracy. The coefficient of variation (CV) value of 5.52% exceeded the limit set by the manufacturer, indicating poor precision. Further evaluation using the Levey-Jenning graph and Westgard's rule indicated a 12s deviation on the fifth day, indicating the possibility of systematic or random errors. The Mindray BC-5380 predilute mode haematology analyser had good accuracy of haemoglobin levels in capillary blood, but poor precision compared to the manufacturer's values.
Pengaruh Stabilitas Sampel Darah EDTA Terhadap Pemeriksaan Hemoglobin dan Hematokrit pada Karyawan Puskesmas Kedung II Metode Otomatis Anjani, Anisa Ayu; Hartinah, Dewi; Kurnia, Shinta Dwi; Rusidah, Yunita; Saputro, Arief Adi
Jaringan Laboratorium Medis Vol. 7 No. 1 (2025): May 2025
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v7i1.12872

Abstract

This study aims to determine the effect of EDTA blood sample stability on hemoglobin (Hb) and hematocrit (Ht) examination results using an automatic method. EDTA blood samples from 38 employees of Kedung II Public Health Center were examined immediately (0 hours), after a 1-hour delay at room temperature (17–210C), and after a 1-hour delay in a refrigerator (2–80C). The results were analyzed using a Two-Way ANOVA test. The study showed no significant differences in hemoglobin and hematocrit levels between the three storage conditions (ρ > 0.05). The average hemoglobin levels were 12.834 g/dL at 0 hours, 12.797 g/dL after 1 hour at room temperature, and 12.829 g/dL after 1 hour in the refrigerator. The average hematocrit levels were 38.484% at 0 hours, 38.321% after 1 hour at room temperature, and 38.434% after 1 hour in the refrigerator. The conclusion of this study is that storing EDTA blood samples for 1 hour, whether at room temperature or in the refrigerator, does not significantly affect hemoglobin and hematocrit examination results.
Perbandingan Nilai Hematokrit Metode Mikrohematokrit dan Metode Automatik Hematology Analyzer Zybio Z3 Menggunakan Darah Vena di Klinik Utama Restu Ibu Jepara Asmawati, Dwi; Hartinah, Dewi; Saputro, Arief Adi
Jaringan Laboratorium Medis Vol. 7 No. 2 (2025): November 2025
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v7i2.12934

Abstract

Pregnant women are one of the populations at risk of contracting Sexually Transmitted Infections (STIs) such as HIV, Syphilis, and Hepatitis B. The prevalence of HIV/AIDS transmission from mother to child is 20-50% where 5-10% occurs during pregnancy. Syphilis transmission is 69-80% and Hepatitis B transmission is more than 90% with the potential for transmission during pregnancy and childbirth being very high. Based on the Regulation of the Minister of Health of the Republic of Indonesia, regulation No. 52 of 2017 concerning the Elimination Program for Transmission of Human Immunodeficiency Virus, Syphilis, and Hepatitis B from Mother to Child or often called "Triple Elimination" aims to stop transmission from mother to child with laboratory examinations on pregnant women at least once during pregnancy. The purpose of this study was to determine the relationship between knowledge and behavior of healthy reproductive age pregnant women in the first trimester at the Lebdosari Health Center. In general, this research method is qualitative with an analytical observational approach and cross-sectional design, namely researchers come and analyze the problem directly. Data collection is carried out at once within a certain time. The population  is pregnant women in the first trimester at the Lebdosari Health Center, with a sample size of 49 people selected based on the Purposive Sampling technique. Data analysis was carried out descriptively and then continued with a bivariate test with the Kendal Tau test because the data was not normally distributed. The results of the study showed that the results of the bivariate test with the Kendal Tau test showed that there was no relationship between knowledge and behavior because the significance value p = 0.888> 0.05 with a positive correlation coefficient value (0.19) which means that there is a positive relationship between knowledge and behavior even though the relationship is weak because the value is less than 1, so that if knowledge increases, behavior will be better. It is recommended that pregnant women are advised to always maintain personal hygiene and implement a clean and healthy lifestyle to prevent infectious diseases.
Co-Authors Achmad Ridwan Anisa Sholikhati Anjani, Anisa Ayu Anny Rosiana Arina Lis Sa'adah Arum Mawati Asmawati, Dwi Atina Hussaana Avira Budianita Avira Budianita Catur Retno Lestari Catur Retno Lestari Dewi Hartinah Dwi Apriliani, Dwi Fania Nurul Khoirunnisa Fathimah Isnina Ghazi, Tafta Hasriyani Hasriyani Indanah Joko Utomo Kurniawati, Eka Milad Naufal Akbar Mu'adzah Mu'adzah Noor Hidayah Nunung Qomariyah Nurul Islamiyah Nurul Islamiyah Okta Yosiana Dewi Prameswari, Fatikha Qurrotu A'yuni Auliya Rahmawaty, Annis rusnoto rusnoto Sa’adah, Arina Lis Septanti, Annisya Luthfi Septina Rahmawati shinta dwi kurnia Shinta Dwi Kurnia Siska, Sri Sukarmin Sukarmin Susi Nurohmi Tanaya, Nindy Taufiqurrachman Nasihun Ulfa, Navira Septiana Yayuk Mundriyastutik Yulisetyaningrum Yunita Rusidah Yunita Rusidah