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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Agrikultura Zuriat Jurnal Agro Jurnal Sains Teh dan Kina AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian J-Dinamika: Jurnal Pengabdian Kepada Masyarakat Jurnal Kajian Budaya dan Humaniora (JKBH) Agrikultura Masyarakat Tani: Jurnal Pengabdian kepada masyarakat
Citra Bakti, Citra
Faculty Of Agriculture, Universitas Padjadjaran
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Economics, Econometrics & Finance Education Environmental Science Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Library & Information Science Mechanical Engineering

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Journal : Agrikultura

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Sensory Test and Molecular Marker Based-Selection for Aromatic Rice in the F3 Progenies Nono Carsono; Amalia Purdianty; Santika Sari; Citra Bakti
Agrikultura Vol 31, No 2 (2020): Agustus, 2020
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/agrikultura.v31i2.27085

Abstract

Aromatic rice is a special type of rice that highly preferred by people in Asia due to the presence of aroma. Aroma in rice is determined by 2-acetyl-1-pyrroline (2AP) compound which is controlled by a recessive fgr gene. A hybridization between cv. Sintanur (aromatic rice) and PTB33 (non-aromatic, resistant to brown planthopper/BPH) has been done in order to develop aromatic rice lines that resistant to BPH. In the F2 progeny, molecular marker-based selection and bioassay for the brown planthopper resistant lines have been carried out; however selection for the aromatic trait has not been performed yet. The objective of this study was to obtain the F3 progeny’s individual with aromatic trait. Sensory test was conducted by KOH 1.7% solution, meanwhile molecular markers applied were ESP (External Antisense Primer), IFAP (Internal Fragrant Antisense Primer), INSP (Internal Non fragrant Sense Primer) and EAP (External Antisense Primer). Eighty-eight plants from two selected (SP#31 and SP#224) F3 lines progenies derived from cv. Sintanur and PTB33 have been evaluated in this study. Detection by molecular markers found seventy-five genotypes (85.23%) were homozygous recessive (aromatic rice) and one was heterozygous (non-aromatic). Eighty-five (96.59%) genotypes were aromatic as detected by sensory test alone. Seventy-two (81.82%) genotypes were categorized as aromatic rice based on sensory test and molecular markers. Due to inconsistency results from each method alone, it is advised both methods to be applied to ensure the reliability and the accuracy since aroma in rice is affected by genetic composition and environment conditions. Selected genotypes will be continued for breeding program in developing aromatic rice with improved agronomic traits.
Evaluasi Kualitas Buah, Komponen Hasil dan Hasil Enam Varietas Tomat Calon Tetua Persilangan Damayanti, Farida; Astri, Ayu; Anas, Anas; Sari, Santika; Bakti, Citra
Agrikultura Vol 35, No 3 (2024): Desember, 2024
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/agrikultura.v35i3.49852

Abstract

Tomat (Solanum lycopersicum L.) merupakan salah satu komoditas hortikultura penting karena pemanfaatannya yang beragam dan merupakan sumber antioksidan, vitamin A, dan C yang tinggi. Upaya perakitan tomat yang memiliki kualitas buah baik, hasil tinggi dan toleran cekaman suhu tinggi dapat dilakukan melalui pembentukan populasi Near Isogenic Lines (NILs). Seleksi tetua recurrent menjadi salah satu tahapan penting dalam pembentukan populasi NILs. Penelitian ini bertujuan untuk melakukan karakterisasi dan seleksi berdasarkan karakter kualitas buah, komponen hasil dan hasil pada enam varietas tomat Indonesia. Percobaan dilakukan di greenhouse Bale Tatanen Padjadjaran menggunakan sistem budidaya hidroponik, pada bulan Juni sampai Desember 2022. Percobaan disusun dalam Rancangan Acak Kelompok (RAK) yang diulang sebanyak empat kali. Perlakuan yang diuji adalah enam varietas tomat yaitu Intan, Opal, Mirah, Berlian, Mutiara, dan Ratna. Karakterisasi kualitas buah dilakukan dengan menggunakan metode destruksi dengan High Performance Liquid Chromatography (HPLC), Spektrofotometer Uv-Vis dan Texture Analyzer. Data dianalisis menggunakan analisis ragam dan uji lanjut Duncan Multiple Range Test (DMRT) taraf nyata 5%. Seleksi calon tetua recurrent dilakukan menggunakan metode Simple Additive Weighting (SAW). Hasil penelitian menunjukkan terdapat perbedaan kualitas buah pada karakter kualitas buah, komponen hasil dan hasil pada keenam varietas tomat Indonesia yang diuji. Varietas Mirah dan Intan terseleksi berdasarkan karakter kualitas buah, komponen hasil dan hasil yang tinggi dengan nilai pembobotan sebesar 0,886 dan 0,871. Kedua varietas berpotensi digunakan sebagai tetua recurrent pada pembentukan populasi NILs tomat toleran cekaman suhu tinggi.
Co-Authors , Witjaksono Ade Ismail Ade Ismail Agung Karuniawan Agus Wahyudin Amalia Purdianty Anas Anas, Anas Anne Nuraini Anne Nurbaity Arini Zahra Azhari Astri, Ayu Dedi Ruswandi Erizon, Meisyela Salsabila Erni Suminar Eva Aprilia Fadhilah, Rifat Farida Damayanti Fitri Widiantini G. A. Wattimena Heri Syahrian, Heri Indrajati, Septian Intan Ratna Dewi Anjarsari Kang, Seung Won Kusumah, Fajar Maulana Wijaya Leni Nuraeni Maulana, Haris Meisyela Erizon Mitalo, Oscar W. Murgayanti Murgayanti Nono Carsono Prayoga, Muhammad Khais Putra, Fitriansyah Rija Sudirja Rudianto, Safira Damayanti Rufaidah, Fathi Santika Sari Santika Sari Sari, Dwi Novanda Shantosa Yudha Siswanto Suseno Amien Syariful Mubarok Wahyudin, Abdillah Azzam Yudithia Maxiselly