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All Journal El-Hayah : Jurnal Biologi Indonesian Journal of Mathematics and Natural Sciences Abdimas Lembaran Ilmu Kependidikan Journal of Biology Education Unnes Science Education Journal Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Biosaintifika: Journal of Biology & Biology Education AL-HAYAT: Journal of Biology and Applied Biology Journal on Education Journal of Innovative Science Education Jurnal Abdimas Mahakam Abdimas Toddopuli: Jurnal Pengabdian Pada Masyarakat Abdimasku : Jurnal Pengabdian Masyarakat Jurnal Abdidas Jurnal Agrotropika Bioscientist : Jurnal Ilmiah Biologi Life Science Prosiding Seminar Nasional Biologi Biosaintifika: Journal of Biology & Biology Education Life Science
Noor Aini Habibah
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Analisis Keanekaragaman Genetik dan Diferensiasi Jati Jawa dan Madura berdasarkan Marka Mikrosatelit untuk Mendukung Fingerprinting Jati Munisyatul Millah; Noor Aini Habibah; Endah Suwarni
Biosaintifika: Journal of Biology & Biology Education Vol 2, No 2 (2010): September 2010
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v2i2.1157

Abstract

Analysis fingerprinting teak is supported by information about teak’s character molecularly. The information is gotten by analyzing 3 primary microsatellite in 46 teak samples that produce total 18 kinds of alel averagely 6 alel per locus. Genetics variety level is shown by Observed Heterozigosity (Ho), Expected Heterozygosity (He), Polimorfi sm Information Content (PIC) and Genetics Differentiation Coeffi cient successively 0.5122; 0.6221; 0.5818; and 0.0629. Kinship connection through dendogram analysis produces coeffi cient resemblance 0.3-1.00 that form 2 groups. However it has not shown inclination of forming group based on origin grows yet. Genetics differentiation analysis indicates different genetics in a population is higher then it is between populations. Analysis result has not found specifi c alel yet but has found 12 specifi c genotipe that is potential for analyzing fi ngerprinting if it relates to important useful genes. Keywords: microsatellite teak, DNA fi ngerprinting
Optimization of In Vitro Conservation Protocol of Carica pubescens Lenne & K. Koch through Medium Concentration, Temperature and Irradiation Duration Decrease Enni Suwarsi Rahayu; Noor Aini Habibah
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 1 (2016): March 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i1.5371

Abstract

Carica pubescens Lenne K. Koch, an endemic species in Dieng mountains, must be conserved. The in vitro conservation has been developed, but sub-culture period needs to be extended. This study aimed to obtain a more efficient in vitro conservation protocol of C. pubescens. The research was carried out experimentally by using a completely randomized factorial design with three factors, namely decreasing in storage medium concentration (75% and 50% of MS medium), temperature (4oC and 8°C), and irradiation duration (8 hours/day and 16 hours/day). Shoots were kept in the storage medium for 6, 9 and 12 months, then their viability were tested by growing them in the regeneration medium. Data were analyzed by Analyses of Variance and Least Significant Difference Test. The results showed that medium concentration of 50% of MS, the temperature of 8 °C, and 16 hours/day of irradiation were able to suppress the C. pubescens growth in vitro storage for six months and could maintain its viability in the regeneration medium. Based on these results the medium concentration of 50% of MS, the temperature of 8 °C and 16 hours/day irradiation can be used for in vitro conservation of C. pubescens without sub-culture for six months. How to CiteRahayu, E., Habibah, N. (2016). Optimization of In Vitro Conservation Protocol of Carica pubescens Lenne K. Koch through Medium Concentration, Temperature and Irradiation Duration Decrease. Biosaintifika: Journal of Biology Biology Education, 8(1), 85-93.
Analisis Keanekaragaman Genetika dan Diferensiasi Jati Jawa dan Madura Berdasarkan Marka Mikrosatelit Untuk Mendukung Fingerprinting Jati Mun Isyatul Millah; Noor Aini Habibah; Endah Suwarni; Amin Rertoningsih
Biosaintifika: Journal of Biology & Biology Education Vol 4, No 2 (2012): September 2012
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v4i2.3929

Abstract

Fingerprinting jati diperlukan untuk melengkapi data base konservasi plasma nutfah jati. Penelitian ini bertujuan untuk mendapatkan informasi keanekaragaman genetika pohon jati berdasarkan marka mikrosatelit dan mengidentifikasi alel spesifik pada lokus tertentu yang dapat menjadi penciri khas jati populasi Jawa dan Madura. Pada penelitian ini dilakukan identifikasi karakteristik jati plus (fingerprinting) secara molekuler menggunakan penanda mikrosatelit. Sampel diambil dari koleksi jati plus di Kebun Benih Klonal (KBK) di Jawa dan dari lokal Areal Produksi Benih (APB) Madura. Sampel diisolasi menggunakan metode CTAB. Amplifikasi DNA menggunakan 3 primer mikrosatelit dan hasilnya divisualisasi pada gel poliakrilamid dengan pewarnaan silver. Hubungan kekerabatan dianalisis melalui program NTSYSpc (Numerical Taxonomy and Multivariate Analysis Sistem) versi 2.01. Hasil analisis menunjukkan tingkat keanekaragaman jati yang tinggi, didukung oleh nilai Observed Heterozigosity (Ho), Expected Heterozygosity (He), Polimorfism Information Content (PIC) dan koefisien diferensiasi genetika berturut-turut 0.5122; 0.6221; 0.5818; dan 0.0629. Hubungan kekerabatan melalui analisis dendogram menghasilkan koefisien kemiripan 0.3-1.00. Nilai keanekaragaman dalam populasi (HS), antar populasi (DST), dan nilai diferensiasi (G) berturut-turut adalah 0.5817, 0.0391, dan 0.0629. Dari hasil analisis disimpulkan bahwa tingkat keanekaragaman jati di Jawa dan Madura termasuk tinggi dan ada indikasi perbedaan genetika di dalam populasi lebih tinggi dibanding antar populasi.Fingerprinting teak is required to complete the data base teak germplasm conservation. This research aimed to obtain information about the genetic diversity of teak based on microsatellite markers and identify specific allele at a particular locus can be distinctive identity identifier population of Java and Madura. In this research is to identify the characteristics of teak plus (fingerprinting) molecularly using microsatellite markers. Samples were taken from the collection of teak plus in Kebun Benih Klonal in Java and from Areal Produksi Benih Madura. Samples were isolated using the CTAB method. Amplification of DNA using microsatellite primer 3 and the results are visualized on a polyacrylamide gel by silver staining. Kinship analyzed through NTSYSpc program (Numerical Taxonomy and Multivariate Analysis System) version 2:01. The analysis showed a high level of diversity teak, supported by Heterozigosity Observed value (HO), Expected Heterozygosity (He), polymorphisms Information Content (PIC) and genetic differentiation coefficient 0.5122 respectively; 0.6221; 0.5818; and 0.0629. Kinship through analysis dendogram generate similarity coefficient 0.3-1.00. The value of diversity in the population (HS), between populations (DST), and the value of differentiation (G) respectively are 0.5817, 0.0391, and 0.0629. From the analysis concluded that the level of diversity of teak in Java and Madura are high, and there are indications of genetic differences in the population is higher than among the population.
Optimasi Konsentrasi 2,4-D, Ba, dan Lama Penyinaran untuk Memacu Regenerasi Tunas dari Kalus Kedelai Intan Kristanti; Noor Aini Habibah; Lina Herlina
Biosaintifika: Journal of Biology & Biology Education Vol 5, No 1 (2013): March 2013
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v5i1.3923

Abstract

Alternatif untuk mengatasi kualitas kedelai yang rendah yaitu perbaikan sifat genetik. Penelitian ini bertujuan untuk mengetahui pengaruh konsentrasi 2,4-Dichlorophenoxyacetic Acid (2,4-D) dan Benzyl Adenine (BA) serta lama penyinaran dan interaksinya terhadap regenerasi tunas dari kalus kedelai, dan untuk menentukan interaksi faktor-faktor yang paling optimal dalam regenerasi tunas dari kalus kedelai. Konsentrasi 2,4-D dan BA masing-masing terdiri dari 4 taraf (0 ppm; 3 ppm; 6 ppm; 9 ppm) dan 2 taraf lama penyinaran (24 jam dan 0 jam). Analisis menggunakan ANAVA tiga arah dan uji lanjut Duncan. Parameter yang diamati adalah waktu muncul tunas, panjang tunas, jumlah tunas, dan persentase kalus membentuk tunas. Hasil menunjukkan bahwa konsentrasi BA dan lama penyinaran mempengaruhi regenerasi tunas dari kalus kedelai, sedangkan konsentrasi 2,4-D tidak berpengaruh signifikan terhadap regenerasi tunas. Konsentrasi BA yang paling optimal adalah 3 ppm dan lama penyinaran yang optimal adalah kondisi 0 jam. Interaksi konsentrasi BA, 2,4-D dan lama penyinaran berpengaruh terhadap regenerasi tunas terutama pada banyaknya jumlah tunas yang dihasilkan. Perlakuan BA 3 ppm + 2,4-D 6 ppm + 24 jam adalah perlakuan yang optimal dalam meregenerasi tunas dengan jumlah kalus yang banyak. Berdasarkan hasil penelitian disarankan untuk meregenerasi kalus menggunakan ZPT BA dan 2,4-D dan Lama penyinaran.Genetic trait improvement is a way to overcome the low quality soybean. The aim of this research is to determine the effect of 2,4-Dichlorophenoxyacetic Acid (2,4-D) and Benzyl Adenine (BA) concentration and long irradiation and their interaction on bud regeneration from callus soybean, and to determine the interaction of the factors that most optimal in bud regeneration from callus soybean. 2,4-D and BA concentration each consisting of 4 levels (0 ppm; 3 ppm; 6 ppm; 9 ppm) and 2 levels long irradiation (24 hours and 0 hour). Analysis using a three -way ANAVA and Duncan test further. Parameters measured were the time appears bud, bud length, number of buds, and the percentage of callus forming buds. The result showed that BA concentration and long irradiation affects the regeneration of shoots from callus soybean, whereas the concentration of 2,4-D had no significant effect on bud regeneration. The most optimal concentration of BA is 3 ppm and optimal long irradiation is the condition 0 hours. BA concentration, 2,4-D concentration and long irradiation interaction effect on the regeneration of buds mainly on the number of buds produced. Treatment BA 3 ppm + 2,4-D 6 ppm + 24 hours is the optimal treatment in regenerating buds the number of callus that many. Based on the research results suggested to regenerate callus using ZPT BA and 2,4-D and long irradiation.
Flavonoid Production in Callus Cultures from Mesocarp of Stelechocarpus burahol Noor Aini Habibah; Sukarti Moeljopawiro; Kumala Dewi; Ari Indrianto
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 2 (2016): September 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i2.6632

Abstract

Stelechocarpus burahol is one of the medicinal plants that contains flavonoids. The study was carried out to know flavonoid production of cultures in vitro S. burahol from mesocarp explants. Mesocarp explants were cultured on MS medium containing different combination and concentration of plant growth regulators i.e. picloram (5, 7.5 and 10 mg/L) and 2, 4-D (10, 15 and 20 mg/L) under dark condition. Induction of callus formation started on the 20.29th to the 29.86th days. Medium supplemented with Picloram and dark state proved to be the best condition for optimum callus induction from mesocarp explants of S. burahol. Callus grown on medium with the addition of 7.5 mg/l Picloram produces the highest flavonoid. The maximum production of the secondary metabolite was obtained from 8 weeks old callus. However, by the time of callus ageing, its output has declined. It could be concluded that callus cultures from mesocarp S. burahol can be used for flavonoid production. How to CiteHabibah, N. A., Moeljopawiro, S. Dewi, K. Indrianto, A. (2016). Flavonoid Production in Callus Cultures from Mesocarp of Stelechocarpus burahol. Biosaintifika: Journal of Biology Biology Education, 8(2), 214-221.
Development of In Vitro Conservation Medium of Carica pubescens Lenne & K. Koch through Nutrients Concentration Reduction and Osmoregulator Addition Enni Suwarsi Rahayu; Noor Aini Habibah; Lina Herlina
Biosaintifika: Journal of Biology & Biology Education Vol 7, No 1 (2015): March 2015
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v7i1.3538

Abstract

Carica pubescens Lenne K. Koch  is a rare species that need to be conserved.  The research aim was to develop a slow growth method of in vitro conservation medium through determining some effects of nutrition decreasing availability in the conservation medium on growth and survival of explants. Establishing epicotyls reached from in vitro seed germination was grown on diluted basic medium of 75% MS (Murashige and Skoog), 50% MS, 25% MS, while osmoregulator compound of mannitol and sorbitol was added to the full MS medium in several concentrations. The treatments were arranged in a completely randomized design with three replications. The epicotyls were grown at storage medium for 12 and 16 weeks, then their survival were evaluated at regeneration medium and rooting medium.  The diluted basic medium and osmoregulator addition were evaluated for its influence in retarding the culture growth in terms of improved survival over the period of 16 weeks.  Data analyzed by one way analysis of variance and Duncan’s multiple range test. The results showed that the decreasing of nutrition concentration suppressed the growth of the epicotyls until 16 weeks after conservation. Epicotyls taken from 16 weeks after conservation could grow on the regeneration medium. The best  survival  was shown by the 75% MS, 50% MS and supplementing of  20 g/l mannitol treatments. Based on these results, 50% MS medium  is recommended  for storage C. pubescens for 16 weeks with no sub-culture.Carica pubescens Lenne K. Koch (karika dieng) merupakan tanaman yang langka sehingga perlu dilestarikan. Penelitian ini bertujuan untuk memperoleh medium penyimpanan in vitro dengan teknik pertumbuhan minimal dengan mengamati pengaruh penurunan ketersediaan nutrisi dalam medium terhadap penurunan pertumbuhan dan daya tumbuh eksplan.Eksplan berupa epikotil kecambah in vitro. Perlakuan penurunan ketersediaan nutrisi dilakukan melalui reduksi konsentrasi nutrisi dari medium Murashige Skoog (MS) dan penambahan osmoregulator (manitol dan sorbitol) dengan berbagai konsentrasi. Penelitian dilakukan dengan rancangan acak lengkap satu faktor dengan tiga ulangan. Epikotil dipelihara dalam medium penyimpanan selama 12 dan 16 minggu, kemudian dievaluasi daya tumbuhnya dengan memelihara dalam medium regenerasi dan medium pengakaran. Data dianalisis dengan analisis varians satu arah dan uji Duncan. Hasil penelitian menunjukkan bahwa penurunan kecepatan penyerapan nutrisi berpengaruh terhadap pertumbuhan eksplan. Epikotil yang telah disimpan selama 12 minggu dan 16 minggu dan ditumbuhkan kembali pada medium regenerasi masih dapat tumbuh dengan intensitas tertinggi pada perlakuan pengenceran 50% MS dan 75% MS, serta penambahan manitol 20 g/l. Komposisi medium ini dapat dimanfaatkan untuk penyimpanan karika dieng selama 16 minggu tanpa sub-kultur. Konsentrasi medium MS 50% direkomendasikan untuk digunakan dalam penyimpanan C. pubescens selama 16 minggu tanpa sub-kultur.
Callogenesis of Dayak Onion (Eleutherine palmifolia) Bulb in response of Picloram, 2,4-D, and Kinetin Noor Aini Habibah; Ananda Lutfiah; Alin Liana; Woro Anindito Sri Tunjung; Meti Indrowati; Furzani Pa’ee
Biosaintifika: Journal of Biology & Biology Education Vol 15, No 2 (2023): August 2023
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v15i2.46501

Abstract

Dayak onion contains bioactive compounds that are traditionally used for medicine. The production of bioactive compounds can be increased through callus culture. This study aims to analyze the effect of the combination of plant growth regulator on the growth of Dayak bulb callus. The design of this study used a completely randomized factorial design with 2 factors. The first factor is the concentration of auxin. While the second factor is the concentration of kinetin. The parameters observed include the percentage of callus, the time of callus formation, fresh weight, dry weight, and callus morphology. In this study, a callus growth curve was also made to determine the best harvest time. The results showed that highest callus fresh weight was obtained in the P3K1 treatment. The percentage of callus formation in the picloram + kinetin treatment was higher than in the 2,4-D + kinetin treatment. The time of callus formation occurred in the combination of picloram + kinetin faster than the 2.4-D + kinetin treatment. The callus color is diverse,  while the texture of the entire callus is compact. The best callus induction medium for Dayak onion bulbs is to use picloram 2-4 ppm + kinetin0.025-0.5 ppm. The results of this study provide optimal growth regulatory information for the induction of dayak onion callus that has never been reported before. This information can be the basis for the development of methods of production of bioactive compounds from dayak onions through callus culture.
PERTUMBUHAN KALUS DAUN DEWA [Gynura procumbens (Lour) Merr.] DENGAN KOMBINASI 2,4-DICHLOROPHENOXYACETIC ACID DAN KINETIN SECARA INVITRO Samkhatin Khaniyah; Noor Aini Habibah; Sumadi -
Biosaintifika: Journal of Biology & Biology Education Vol 4, No 2 (2012): September 2012
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v4i2.2274

Abstract

Tanaman daun dewa [Gynura procumbens (Lour) Merr.] berguna untuk menurunkan kadar kolesterol darah, mengobati diabetes, tumor, dan sebagai obat anti kanker. Daun dewa mengandung senyawa metabolit sekunder yaitu flavonoid, saponin, dan minyak atsiri. Oleh karena itu, perlu adanya kultur in vitro yang diharapkan mampu meningkatkan jumlah metabolit sekunder, maka perlu dilakukan penelitian tentang induksi kalusdengan penambahan kombinasi 2,4-Dichlorophenoxyacetic acid dan kinetin yang tepat dapat digunakan untuk menginduksi kalus daun dewa. Tujuan penelitian ini untuk mengkaji pengaruh konsentrasi 2,4-D dan Kinetin serta interaksi keduanya terhadap induksi kalus daun dewa. Analisis menggunakan anava dua arah dan uji lanjut Duncan. Parameter yang diamati adalah parameter persentase eksplan yang hidup, persentase berkalus, berat basah, kering kalus, serta tekstur dan warna kalus . Dari Uji Jarak Berganda Duncan diperoleh hasil tertinggi pada konsentrasi 2,4-Dichlorophenoxyacetic acid 0.5 ppm dan konsentrasi kinetin 1 ppm sebesar 33.33% pada parameter persentase berkalus.Daun dewa [Gynura procumbens (Lour). Merr.] plant can be used to lower the blood cholesterol levels, to treat diabetes and tumors, and may be used as an anti-cancer drug. Daun dewa plant produces of secondary metabolites such as flavonoids, saponins, and essential oils. An in vitro culture is a necessity to increase the quantity of secondary metabolites using callus induction with the addition of a combination of 2,4-Dichlorophenoxyacetic acid and kinetin to induce daun dewa callus. The research will ecamine the influence of 2,4-Dichlorophenoxyacetic acid and Kinetin in various concentrations and their interaction on callus induction of daun dewa. Data were analyzed using advanced two-way anova and Duncan test. The test showed that the highest yeield was obtained from the combination of 0.5 ppm 2,4-D and 1 ppm kinetin, where 33.33% of plants had callus.
Implementation of Educational Supervision during the Pandemic Period at Middle School in Kebumen District Vira Ayuningrum; Siti Alimah; Noor Aini Habibah
Journal of Innovative Science Education Vol 12 No 2 (2023): August 2023
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jise.v12i2.71884

Abstract

This study aims to describe the implementation of the principal's academic supervision in Kebumen District Middle School which is carried out by the school principal. The approach used is a qualitative approach with a case study design. The collection of data used in this study was carried out through interviews, observation and documentation. Data analysis, namely the steps of data reduction, data presentation, and drawing conclusions. The results of the study show that the implementation of school principals' academic supervision during the pandemic: (1) Efforts by school principals to prepare online learning, by motivating teachers; (2) the principal's academic supervision program, namely individual supervision; (3) The school principal's academic supervision strategy, namely individual supervision through scheduled supervision activities in odd and even semesters; (4) Supporting school principals' academic supervision, namely school principals carrying out academic supervision aimed at increasing teacher motivation as well as providing adequate facilities and infrastructure to carry out supervision during the pandemic.
Induksi Kalus dari Eksplan Umbi Bawang Merah (Allium ascalonicum L var. Bima Brebes) Dengan Penambahan BAP dan Pikloram Taqiyyah Rabbani Ramadhan; Noor Aini Habibah
Indonesian Journal of Mathematics and Natural Sciences Vol 46, No 2 (2023): October 2023
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/ijmns.v46i2.47250

Abstract

Kandungan metabolit sekunder dalam umbi bawang merah (Allium ascalonicum) secara efektif dapat diproduksi melalui teknik kultur kalus yang dipengaruhi oleh komposisi auksin dan sitokinin pada media. Penelitian ini bertujuan untuk menganalisis pengaruh konsentrasi benzyl aminopurin (BAP) dan pikloram yang optimal terhadap induksi kalus eksplan umbi bawang merah. Rancangan penelitian adalah rancangan acak lengkap (RAL) dengan dua faktor yaitu konsentrasi BAP dan konsentrasi pikloram, masing-masing dengan konsentrasi 0 ppm, 1 ppm, 2 ppm dan 3 ppm. Eksplan yang diinduksi berupa bagian pelepah kuncup umbi. Kultur dilakukan pada media Murashige and Skoog (MS) dengan pencahayaan 2.000 lux, suhu ±20-25°C, kelembapan 52-58% dan diinkubasi selama 45 hari. Indikator yang diamati meliputi waktu muncul kalus, persentase berkalus dan morfologi kalus. Data waktu muncul kalus dan persentase berkalus dianalisis dengan uji Kruskall-Wallis dan diuji lanjut dengan uji Dunn. Data morfologi kalus dianalisis secara deskriptif. Hasil penelitian menunjukkan bahwa konsentrasi BAP tidak berpengaruh signifikan terhadap waktu muncul kalus dan persentase tumbuh kalus sedangkan konsentrasi pikloram berpengaruh signifikan terhadap kedua parameter tersebut. Rerata waktu muncul kalus tercepat yaitu 8,6 hst (hari setelah tanam) dicapai pada perlakuan 2 ppm BAP + 3 ppm pikloram dengan persentase tumbuh kalus 100%. Morfologi kalus yang dihasilkan pada sebagian perlakuan bertekstur kompak dengan warna kalus putih kekuningan.
Co-Authors Aini Sa’adah Alin Liana Amalia, Adninta Husnu Amin Rertoningsih Amin Rertoningsih, Amin Ananda Lutfiah Andin Irsadi Anindya Ardiansari Anisa Dewi Sri Puspitasari Ardiansari, Anidya Ari Indrianto Ari Indrianto Ari Yuniastuti Arief, Nurul Huda Am Zen Arif Widiyatmoko, Arif Arumsasi, Fitri Aulia, Siska Na'ilil Bahrunnisa, Atiqah Damar Galih Dewi Mustikaningtyas Endah Suwarni Endah Suwarni Endah Suwarni Enni Suwarsi Rahayu Fajar Musafa Farikha, Sabrina Nur Fitri Arum Sari Fitri Arum Sasi, Fitri Arum Fitrianti, Destri Firlidya Furzani Pa’ee Griyani -, Griyani Hapsari Ika Mundiyakin, Ika Intan Kristanti Intan Kristanti, Intan Krispinus Kedati Pukan Kukuh Santoso Kumala Dewi Kumala Dewi Lina Herlina Lina Herlina Lina Herlina Linda Noviyanti Lisdiana Lisdiana Melati, Dinda Meti Indrowati Mia Chalimatur Rosyidah Mualimin Mualimin Muhyidin, Faricha Aulia Mukhtar, Khoirul Mun Isyatul Millah Mun Isyatul Millah, Mun Isyatul Munisyatul Millah Munisyatul Millah Musafa, Fajar Nafia, Rosyida Nugrahaningsih Nugrahaningsih Nugrahaningsih Nugrahaningsih Nur Wijawati Pa’ee, Furzani R Susanti Raula Samsul Amarila, Raula Samsul Rizqi Utami, Rizqi Safitri Safitri Samkhatin Khaniyah Samkhatin Khaniyah Sasabila Mumtaz Shofwan Ridho, Shofwan Sinaga, Na Dame Maulibulung Siti Alimah Siti Harnina Bintari Sri Ambar Sri Ambar Sri Haryani Sri Nanan B Sri Pudyastuti Sri Pudyastuti Sri Puspitasari, Anisa Dewi Sukarti Moeljopawiro Sukarti Moeljopawiro Sumadi - Sumadi - Sumadi - Supriyanto Supriyanto SUTIKNO Sutikno Sutikno Talitha Widiatningrum Taqiyyah Rabbani Ramadhan Titis Purwanti, Titis Tyas Agung Pribadi Vira Ayuningrum Wijawati, Nur Wiwi Isnaeni Wiyanto - Wiyanto Wiyanto Woro Anindito Sri Tunjung Woro Sumarni Wulan Christijanti Y. Ulung Anggraito Y. Ulung Anggraito, Y. Ulung Yustinus Ulung Anggraito