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PENGARUH CAHAYA SERTA KOMBINASI NAA DAN 2,4-D TERHADAP INDUKSI KALUS CABAI MERAH VARIETAS LOTANBAR Mia Chalimatur Rosyidah; Noor Aini Habibah
Prosiding Seminar Nasional Biologi Vol. 11 (2023)
Publisher : Prosiding Seminar Nasional Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar

Cabai merah (Capsicum annuum L) varietas lotanbar merupakan jenis komoditas pertanian potensial bagi kebutuhan pangan Indunesia. Cabai memiliki kandungan senyawa yang berpotensi sebagai obat seperti capsaicinoid, karotenoid, flavonoid dan lain sebagainya. Melalui induksi kalus, dapat dihasilkan senyawa metabolit lebih banyak dibanding dengan kultur biasa. Tujuan penelitian ini adalah menganalisis pengaruh pemberian NAA dan 2,4-D serta cahaya terhadap induksi kalus cabai merah varietas lotanbar. Pada penelitian ini menggunakan rancangan acak kelompok dengan dua faktor yaitu cahaya ( gelap dan terang ) serta NAA ( 0 ; 0,5 ; 1) ppm dan 2,4-D ( 0; 0,5; 1) ppm yang dikombinasikan. Eksplan yang digunakan yaitu hipokotil ±1 cm hasil perkecambahan benih secara in vitro berumur 15 hari. Data dianalisis dengan anova dua arah lalu dilanjutkan dengan uji Tukey. Hasil penelitian menunjukkan tidak ada pengaruh interaksi ZPT dan kondisi pencahayaan terhadap induksi kalus cabai. Faktor ZPT berpengaruh terhadap pertumbuhan kalus, waktu muncul kalus, dan presentase kalus. Kondisi optimal induksi kalus terdapat pada kombinasi ZPT 2,4-D 1 ppm + NAA 0 ppm pada kondisi gelap. Morfologi kalus yang dihasilkan keseluruhan bertekstur remah . Warna kalus pada kondisi terang ialah kuning kehijauan. Sedangkan pada kondisi gelap warna kalus yang dihasilkan putih hingga putih kecoklatan.

Diversifikasi Produk Griya Anggrek Mekar Sari Kalisegoro Gunungpati Semarang Habibah, Noor Aini; Sutikno; Rahayu, Enni Suwarsi; Anggraito, Yustinus Ulung; Sasi, Fitri Arum; Hapsari
Abdimas Toddopuli: Jurnal Pengabdian Pada Masyarakat Vol. 5 No. 1 (2023): Desember 2023
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/atjpm.v5i1.3020

Anggrek merupakan tanaman dengan nilai ekonomis tinggi. Griya anggrek Mekar Sari merupakan salah satu rumah anggrek di wilayah Kalisegoro yang mempunyai potensi besar untuk berkembang. Kegiatan saat ini yang dilakukan di Griya Mekar Sari hanya pemeliharaan dan penjualan anggrek saja. Rutinitas ini memberikan dampak menurunnya antusiasme pengelola. Selain itu tidak beragamnnya produk anggrek yang ditawarkan menyebabkan turunnya omzet penjualan. Sejak berdiri hingga sekarang rerata penjualan anggrek hanya berkisar 4 tanaman perbulan. Jumlah penjualan ini masih rendah dan belum memberikan dampak ekonomi yang berarti bagi anggota Griya Anggrek Mekar Sari. Tingkat penjualan yang rendah antara lain disebabkan karena koleksi anggrek yang masih terbatas dan tidak adanya produk lain yangberkaitan dengan anggrek yang dijual. Perlu adanya suatu terobosan berupa diversifikasi produk yang dapat menarik pembeli dan juga meningkatkan antusime pengelola. Solusi dari berbagai permasalahan yang dialami oleh mitra akan dilakukan bersama-sama antara tim pengusul dengan mitra. Jabaran dari solusi tersebut adalah sebagai berikut: (1) Peningkatan koleksi anggrek. (2) Peningkatan diversifikasi produk anggrek. Diversifikasi produk yang ditawarkan adalah kit pemeliharaan anggrek (berisi media tanam, pot kecil dan bibit anggrek dalam botol kecil) dilengkapi dengan manual penggunaannya, gantungan kunci dan hiasan meja berisi tanaman anggrek, peralatan standar pemeliharaan anggrek, pot cantik homemade, dan juga produk berupa kemasan anggrek yang menarik. Diharapkan diversivikasi produk ini akan meningkatkan omzet penjualan dan juga meningkatkan motivasi bagi pengelola Griya Anggrek Mekar Sari.

Callus Induction from Stem Explants of Binahong (Anredera cordifolia (Ten.) Steenis) with the Addition of Picloram and BAP Aulia, Siska Na'ilil; Habibah, Noor Aini
Al-Hayat: Journal of Biology and Applied Biology Vol 7, No 1 (2024)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v7i1.17799

The many benefits of medicinal plants require development techniques that aim to produce high and diverse secondary metabolite contents. The purpose of this study was to analyze the effect of the concentration of picloram and BAP and determine the optimal concentration in inducing binahong callus. The research design used was a completely randomized design with two factors, namely the combination of picloram and BAP (0, 1, 2, and 3 ppm). The incubation process of binahong callus was carried out for 40 days after planting (DAP). The observed callus growth indicators consisted of days to callus formation (days), percentage of callus explants (%), callus morphology (texture and color), and callus diameter. Data on days to callus formation and frequency of callus explants were analyzed using the Kruskal-Wallis test and further tested by Dunn if there was a significant effect. The results showed that picloram significantly affected the parameters of days to callus formation and the percentage of callus explants, while BAP significantly affected the parameters of days to callus formation and did not significantly affect the percentage of callus explants parameter. At concentrations of 1 ppm, 2 ppm, and 3 ppm, picloram had a significant effect on both the days to callus formation and the percentage of callus explants parameters. BAP at concentrations of 1 ppm, 2 ppm, and 3 ppm only had a significant effect on the parameter of days to callus formation appearance and was not significant in the percentage of callus explants parameter. The interaction between BAP and picloram had a significant effect on the parameters of days to callus formation and the percentage of callus explants. The resulting callus has a compact texture with callus colors ranging from white, and green, to brown. The most optimal concentration in all indicators is 0 ppm BAP + 1 ppm picloram with days to callus formation of 6 DAP, 100% explants form callus, overall callus is white with compact texture, and has a very high callus growth.

Effect of BAP and Picloram on Shoot Induction (Musa acuminata Colla var. Mulu Bebek) Arief, Nurul Huda Am Zen; Rahayu, Enni Suwarsi; Pa’ee, Furzani; Habibah, Noor Aini
Biosaintifika: Journal of Biology & Biology Education Vol. 17 No. 1 (2025): April 2025
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v17i1.19605

Musa acuminata Colla var. Mulu Bebek is a high-quality banana from Ternate, North Maluku. Its conventional cultivation faces challenges due to Fusarium wilt, reducing productivity. In vitro culture offers a solution for rapid, disease-free seedling production. This study aims to examine the effect of BAP and picloram on shoot induction using a CRFD at various concentrations (0–3 ppm) and evaluate the percentage of live explants, emergence time, and number and length of shoots and roots. Results show that 1 ppm picloram accelerates root emergence, while 0 ppm promotes the fastest shoot emergence, the longest shoot and root lengths. 1 ppm BAP increases shoot numbers. The interaction of picloram and BAP has a significant effect on the parameters of the fastest root emergence time, namely 1 ppm picloram + 0 ppm BAP, 1 ppm and 3 ppm, the fastest shoot emergence time, namely without the addition of PGR, and the longest shoot length, 0 ppm picloram + 2 ppm BAP. The combination of 0 ppm picloram + 1 ppm BAP is the optimal condition for a percentage of 100% live explants, the fastest time for root emergence, the highest number of shoots and roots, the longest shoot and root length and the optimal condition for the fastest time for shoot emergence is 0 picloram + 0 ppm BAP. This study provides insight into the role of BAP and picloram in inducing banana shoots that has not been reported before. The micropropagation media formula obtained in this study is an effort to improve the quality of healthy banana seedlings in the future.

Callus Induction from Stem Explants of Binahong (Anredera cordifolia (Ten.) Steenis) with the Addition of Picloram and BAP Aulia, Siska Na'ilil; Habibah, Noor Aini
Al-Hayat: Journal of Biology and Applied Biology Vol. 7 No. 1 (2024)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v7i1.17799

The many benefits of medicinal plants require development techniques that aim to produce high and diverse secondary metabolite contents. The purpose of this study was to analyze the effect of the concentration of picloram and BAP and determine the optimal concentration in inducing binahong callus. The research design used was a completely randomized design with two factors, namely the combination of picloram and BAP (0, 1, 2, and 3 ppm). The incubation process of binahong callus was carried out for 40 days after planting (DAP). The observed callus growth indicators consisted of days to callus formation (days), percentage of callus explants (%), callus morphology (texture and color), and callus diameter. Data on days to callus formation and frequency of callus explants were analyzed using the Kruskal-Wallis test and further tested by Dunn if there was a significant effect. The results showed that picloram significantly affected the parameters of days to callus formation and the percentage of callus explants, while BAP significantly affected the parameters of days to callus formation and did not significantly affect the percentage of callus explants parameter. At concentrations of 1 ppm, 2 ppm, and 3 ppm, picloram had a significant effect on both the days to callus formation and the percentage of callus explants parameters. BAP at concentrations of 1 ppm, 2 ppm, and 3 ppm only had a significant effect on the parameter of days to callus formation appearance and was not significant in the percentage of callus explants parameter. The interaction between BAP and picloram had a significant effect on the parameters of days to callus formation and the percentage of callus explants. The resulting callus has a compact texture with callus colors ranging from white, and green, to brown. The most optimal concentration in all indicators is 0 ppm BAP + 1 ppm picloram with days to callus formation of 6 DAP, 100% explants form callus, overall callus is white with compact texture, and has a very high callus growth.

Callus Induction from Stem Explants of Binahong (Anredera cordifolia (Ten.) Steenis) with the Addition of Picloram and BAP Aulia, Siska Na'ilil; Habibah, Noor Aini
Al-Hayat: Journal of Biology and Applied Biology Vol. 7 No. 1 (2024)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v7i1.17799

The many benefits of medicinal plants require development techniques that aim to produce high and diverse secondary metabolite contents. The purpose of this study was to analyze the effect of the concentration of picloram and BAP and determine the optimal concentration in inducing binahong callus. The research design used was a completely randomized design with two factors, namely the combination of picloram and BAP (0, 1, 2, and 3 ppm). The incubation process of binahong callus was carried out for 40 days after planting (DAP). The observed callus growth indicators consisted of days to callus formation (days), percentage of callus explants (%), callus morphology (texture and color), and callus diameter. Data on days to callus formation and frequency of callus explants were analyzed using the Kruskal-Wallis test and further tested by Dunn if there was a significant effect. The results showed that picloram significantly affected the parameters of days to callus formation and the percentage of callus explants, while BAP significantly affected the parameters of days to callus formation and did not significantly affect the percentage of callus explants parameter. At concentrations of 1 ppm, 2 ppm, and 3 ppm, picloram had a significant effect on both the days to callus formation and the percentage of callus explants parameters. BAP at concentrations of 1 ppm, 2 ppm, and 3 ppm only had a significant effect on the parameter of days to callus formation appearance and was not significant in the percentage of callus explants parameter. The interaction between BAP and picloram had a significant effect on the parameters of days to callus formation and the percentage of callus explants. The resulting callus has a compact texture with callus colors ranging from white, and green, to brown. The most optimal concentration in all indicators is 0 ppm BAP + 1 ppm picloram with days to callus formation of 6 DAP, 100% explants form callus, overall callus is white with compact texture, and has a very high callus growth.

Pengaruh Konsentrasi Kinetin dan Pikloram Terhadap Induksi Tunas Vanili (Vanilla planifolia Andrews.) Secara In Vitro Fitrianti, Destri Firlidya; Habibah, Noor Aini
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 3 (2025): September
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i3.16593

This study aimed to analyze the effects of combining kinetin and picloram on the in vitro shoot development of vanilla. The research was conducted at the Plant Tissue Culture Laboratory, Faculty of Mathematics and Natural Sciences, Universitas Negeri Semarang, from July to November 2024, using a Completely Randomized Design one factorial with 16 treatment combinations and five replications. One nodal explants were taken from the 1st to 6th of Vania variety vanilla plantlets. Parameters observed included days to shoot emergence, shoot growth percentage, shoot length, root length, number of roots, and number of leaves. Data were analyzed using the Kruskal-Wallis test. The results showed that the combination of kinetin and picloram had a significant effect on all observed growth parameters. The treatment without the addition of plant growth regulators (kinetin 0 ppm + picloram 0 ppm) produced the best results across all parameters. Other treatments without picloram also exhibited good growth in at least three main parameters. Therefore, the combination of 0 ppm kinetin and 0 ppm picloram was the most optimal treatment for supporting the in vitro growth of vanilla explants.

Optimalisasi Induksi Kalus Tebu (Saccharum officinarum L.) pada Media Murashige and Skoog dengan Penambahan Pikloram dan Kinetin Nafia, Rosyida; Habibah, Noor Aini
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 3 (2025): September
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i3.17030

This study aimed to analyze the effect of combined picloram and kinetin treatments on callus induction from young leaf roll explants of sugarcane variety Bululawang. The experiment was conducted using a completely randomized design with one factor consisting of 16 treatment combinations of picloram (0, 2, 4, and 6 ppm) and kinetin (0, 0.5, 1, and 1.5 ppm). The observed parameters included the time of callus initiation, percentage of explants forming callus, fresh weight, dry weight, and callus morphology. The results showed that the addition of picloram and kinetin combinations did not significantly affect the time of callus initiation but significantly increased the percentage of explants forming callus (averaging 86.72%) compared to the control (12.50%). The highest fresh and dry weights of callus were recorded at the treatment of 4 ppm picloram + 1 ppm kinetin, with a fresh weight of 0.4412 g and a dry weight of 0.0650 g. Callus morphology was predominantly compact in texture with a yellow to yellow-reddish color. The combination of picloram and kinetin effectively increased the percentage of callus induction, fresh weight, and dry weight of callus in sugarcane, indicating its potential application in tissue culture and secondary metabolite production.

Pemberdayaan Umkm 'NOLi' Melalui Strategi Diversifikasi Produk dan Transformasi Digital Untuk Penguatan Ekonomi Lokal Anggraito, Yustinus Ulung; Noor Aini Habibah; Ardiansari, Anidya; Irsadi, Andin; Arumsasi, Fitri
Abdimas Toddopuli: Jurnal Pengabdian Pada Masyarakat Vol. 7 No. 1 (2025): Volume 7 No 1, Desember 2025
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/atjpm.v7i1.6857

Usaha keripik pisang “Noli” di Desa Kebondalem, Kec. Jambu, Kab. Semarang, Jawa Tengah memproduksi keripik pisang asin dan manis, belum memiliki varian baru. Kemasan yang digunakan sering bocor dan keripik melempem. Daya simpan keripik pisang terbatas dan mengalami ketengikan. Manajemen usaha masih dilakukan secara konvensional dan lantai tempat pencucian masih berlantai semen, terkesan kurang higienis. Tujuan kegiatan ini adalah menambah varian produk keripik pisang, memperbaiki manajemen keuangan, teknik pengemasan, dan tempat pencucian agar lebih bersih. Sekitar 75% kegiatan menggunakan metode praktik, sedangkan 25% berupa kegiatan informatif, diskusi, dan tanya jawab. Hasil kegiatan ini adalah pelaku UMKM berhasil membuat keripik pisang coklat bubuk, coklat lumer, matcha, red velvet, dan tiramisu, serta mengemasnya menjadi layak jual di toko oleh-oleh. Pelaku UMKM telah mampu membuat manajemen keuangan digital menggunakan aplikasi “Akutansiku” dan tempat usaha yang lebih bersih. Pelaku UMKM juga sudah mampu mengoperasikan mesin peniris minyak. Kesimpulan dari kegiatan ini adalah pelaku UMKM “Noli” berhasil memdiversifikasi produk keripik pisang, lebih melek manajemen keuangan digital, terampil mengemas produk, dan meningkatkan kebersihan tempat kerja, yang diharapkan dapat menguatkan ekonomi lokal, sehingga mendukung pencapaian Tujuan Pembangunan Berkelanjutan ke-8, 9, dan 12. Kata kunci: diversifikasi; keripik pisang; manajemen keuangan digital; UMKM

Effect of Chitosan Elicitor Addition and Elicitation Duration on the Growth and Antioxidant Activity of Shallot (Allium ascalonicum L.) Callus Muhyidin, Faricha Aulia; Habibah, Noor Aini
El-Hayah:Jurnal Biologi Vol 10, No 3 (2025): EL-HAYAH (VOL 10, NO 3 September 2025)
Publisher : Program Study of Biology, Science and Technology Faculty, UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v10i3.35387

Shallot (Allium ascalonicum L.) contains various active secondary metabolites such as phenolics, flavonoids, quercetin, saponins, and anthocyanins, which function as natural antioxidants to protect the body from free radicals. Callus culture with the addition of elicitors is an effective method to produce shallot callus with enhanced secondary metabolite content compared to the original plant through manipulation of biosynthetic pathways. This study aimed to determine the most optimal combination of chitosan concentration and elicitation duration to enhance the growth and antioxidant activity of shallot bulb callus in vitro. A Completely Randomized Design (CRD) was used with 12 treatment combinations (chitosan at 50, 100, 150, and 200 mg/L for 2, 4, and 6 days) and 1 control treatment. The results showed that the elicitation combination had no significant effect on fresh weight but significantly affected dry weight and antioxidant activity of the callus. The control treatment was the most optimal for callus growth, yielding the highest average biomass (0.07 grams). Meanwhile, the 2 days + 200 mg/L combination treatment was the most optimal for increasing the antioxidant activity of the callus, producing the highest inhibition value (35.65%). Callus morphology in all treatments exhibited compact texture and varied shades of yellow. This study demonstrates that chitosan elicitor is effective in enhancing the antioxidant activity of A. ascalonicum callus and is therefore recommended for further phytochemical and biotechnological development.

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