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All Journal International Journal of Evaluation and Research in Education (IJERE) HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Ilmu Pertanian Indonesia Jurnal Akuakultur Indonesia Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences BIOTROPIA - The Southeast Asian Journal of Tropical Biology Journal of Tropical Soils Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati J-Simbol: Jurnal Magister Pendidikan Bahasa dan Sastra Indonesia Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Jurnal Sumberdaya Hayati BERITA BIOLOGI Jurnal Biodjati BIOEDUSCIENCE Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Menara Perkebunan Community Development Journal: Jurnal Pengabdian Masyarakat Jurnal Syntax Imperatif : Jurnal Ilmu Sosial dan Pendidikan Lugawiyyat Caraka : Indonesia Journal of Communication MADURANCH: JURNAL ILMU PETERNAKAN Jurnal Ilmiah Sains Jurnal Ventilator: Jurnal Riset Ilmu Kesehatan dan Keperawatan Jurnal Penelitian Pendidikan Indonesia Vitamin: Jurnal Ilmu Kesehatan Umum Retorika: Jurnal Komunikasi, Sosial dan Ilmu Politik
Hamim Hamim
Department Of Biology, Faculty Of Mathematics And Natural Sciences, IPB University, Kampus IPB Darmaga, 16680 Bogor, Indonesia
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mathematics Nursing Physics Public Health Social Sciences Veterinary Other

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The expression of OsPLA2-III and OsPPO genes in rice (Oryza sativa L.) under Fe toxicity stress Turhadi, .; Hamim, .; Ghulamahdi, Munif; Miftahudin, .
Journal of Tropical Life Science Vol 11, No 2 (2021)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Lipid is an important biomolecule in plant because of their structural and functional roles in plant cells. Moreover, lipid could be a signal molecule in defense system of plants under biotic and abiotic stresses. Detoxification process of toxic substances, which is one of the defense system and controlled by various genes, plays an important role in tolerance mechanism in plant under stress conditions, including iron (Fe) toxicity. Plants develop various tolerance strategies to cope the Fe toxicity. The objective of this research was to investigate the expression of OsPLA2-III and OsPPO genes under Fe stress condition. Two-weeks-old seedlings of two rice varieties, i.e., IR64 (Fe-sensitive toxicity) and Pokkali (Fe-tolerant toxicity) were used. Both varieties were treated with 1 (control) and 400 ppm of Fe in the form of FeSO4.7H2O in the nutrient culture solution. The leaf bronzing, chlorophyll content, and relative expression of OsPLA2-III and OsPPO genes were observed. An in-silico study was also performed to predict the interaction between OsPLA2-III and OsPPO proteins. The results showed that the Fe toxicity induced leaf bronzing, reduced leaf chlorophyll content, and increased the OsPLA2-III and OsPPO gene expression levels. Both genes are suggested to have a role in plant tolerance mechanism during Fe toxicity stress via lipid signaling pathway.
Expression of TAD1 (Tillering & Dwarf1) Gene in Hawara Bunar and IR64 Rice Cultivars: Expression of TAD1 (Tillering & Dwarf1) Gene in Hawara Bunar and IR64 Sindiya, Vita; Fendiyanto, Miftahul Huda; Hamim, Hamim; Miftahudin , Miftahudin
Journal of Tropical Life Science Vol. 14 No. 3 (2024): In Press
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.03.11

Abstract

Rice cv. Hawara Bunar is a local rice cultivar tolerant to aluminum (Al) and drought stress. However, the cultivar has inferior characteristics, such as a tall habitus and a small number of tillers, making the cultivar agronomically unattractive. Many genes control plant height and tiller number; one is the TAD1 gene. Analysis of gene expression in two contrasting rice cultivars for both characters is a prerequisite for selecting certain genes for gene editing targets. This study aimed to analyze the gene expression of the TAD1 in rice cv. IR64 and Hawara Bunar, and to construct a phylogenetic tree of genes that regulate rice plant height and tiller number. Gene expression analysis was conducted using the qRT-PCR technique, while the phylogenetic tree was constructed based on the Neighbor-Joining method using PAUP4 software. The results showed TAD1 gene expression in the tillering phase of rice cv. Hawara Bunar is higher than the cv. IR64. The gene expression level in both cultivars corresponds to the plant height and tiller number characters in both rice cultivars. Phylogenetic analysis showed that the TAD1 gene clustered with genes that cause the rice to have a tall habitus with few tillers. The results of a transcriptome meta-analysis reinforced the phylogenetic tree, which shows that the TAD1 gene was found in a group of downregulated genes based on the volcano plots. Therefore, the TAD1 gene can be selected as a target gene for editing in rice cv. Hawara Bunar to obtain superior characters.
Expression of TAD1 (Tillering & Dwarf1) Gene in Hawara Bunar and IR64 Rice Cultivars: Expression of TAD1 (Tillering & Dwarf1) Gene in Hawara Bunar and IR64 Sindiya, Vita; Fendiyanto, Miftahul Huda; Hamim, Hamim; Miftahudin , Miftahudin
Journal of Tropical Life Science Vol. 14 No. 3 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.03.11

Abstract

Rice cv. Hawara Bunar is a local rice cultivar tolerant to aluminum (Al) and drought stress. However, the cultivar has inferior characteristics, such as a tall habitus and a small number of tillers, making the cultivar agronomically unattractive. Many genes control plant height and tiller number; one is the TAD1 gene. Analysis of gene expression in two contrasting rice cultivars for both characters is a prerequisite for selecting certain genes for gene editing targets. This study aimed to analyze the gene expression of the TAD1 in rice cv. IR64 and Hawara Bunar, and to construct a phylogenetic tree of genes that regulate rice plant height and tiller number. Gene expression analysis was conducted using the qRT-PCR technique, while the phylogenetic tree was constructed based on the Neighbor-Joining method using PAUP4 software. The results showed TAD1 gene expression in the tillering phase of rice cv. Hawara Bunar is higher than the cv. IR64. The gene expression level in both cultivars corresponds to the plant height and tiller number characters in both rice cultivars. Phylogenetic analysis showed that the TAD1 gene clustered with genes that cause the rice to have a tall habitus with few tillers. The results of a transcriptome meta-analysis reinforced the phylogenetic tree, which shows that the TAD1 gene was found in a group of downregulated genes based on the volcano plots. Therefore, the TAD1 gene can be selected as a target gene for editing in rice cv. Hawara Bunar to obtain superior characters.
The expression of OsPLA2-III and OsPPO genes in rice (Oryza sativa L.) under Fe toxicity stress Turhadi, .; Hamim, .; Ghulamahdi, Munif; Miftahudin, .
Journal of Tropical Life Science Vol. 11 No. 2 (2021)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.11.02.10

Abstract

Lipid is an important biomolecule in plant because of their structural and functional roles in plant cells. Moreover, lipid could be a signal molecule in defense system of plants under biotic and abiotic stresses. Detoxification process of toxic substances, which is one of the defense system and controlled by various genes, plays an important role in tolerance mechanism in plant under stress conditions, including iron (Fe) toxicity. Plants develop various tolerance strategies to cope the Fe toxicity. The objective of this research was to investigate the expression of OsPLA2-III and OsPPO genes under Fe stress condition. Two-weeks-old seedlings of two rice varieties, i.e., IR64 (Fe-sensitive toxicity) and Pokkali (Fe-tolerant toxicity) were used. Both varieties were treated with 1 (control) and 400 ppm of Fe in the form of FeSO4.7H2O in the nutrient culture solution. The leaf bronzing, chlorophyll content, and relative expression of OsPLA2-III and OsPPO genes were observed. An in-silico study was also performed to predict the interaction between OsPLA2-III and OsPPO proteins. The results showed that the Fe toxicity induced leaf bronzing, reduced leaf chlorophyll content, and increased the OsPLA2-III and OsPPO gene expression levels. Both genes are suggested to have a role in plant tolerance mechanism during Fe toxicity stress via lipid signaling pathway.
Co-Authors , Triadiati Abdul Aziz Ahmad Barizi Alex Hartana ALEX HARTANA Alex Hartana Anhikmah, Anhikmah Anja Meryandini Aris Tjahjoleksono Aris Tri Wahyudi Auliya, Fatikhatur Rizki BEKTI KURNIAWATI Cholili, Abd Hamid Deden Saprudin Devi Media Siska Dian Hendrayanti Didy Sopandie DWI ANDREAS SANTOSA Eddy Supriyono Ekoputro, Widiyatmo Faidah Faidah Fendiyanto, Miftahul Huda Hadisunarso Hadisunarso Hirmas Fuady Putra HIRMAS FUADY PUTRA, HIRMAS FUADY Iman Rusmana Inayatur Rosyidah Indah Puspita SARI khayati, Eliya Nur Kukuh Nirmala Laelasari Laksmita Prima SANTI Luluk Setyaningsih Lutfiani Lutfiani Ma’arif, A. Samsul Ma’arif Miftahuddin Miftahudin , Miftahudin Miftahudin . Miftahudin, M. MISHBAHUDDIN, MOHAMAD Mubasyiroh, Mubasyiroh Muhammad Jusuf Munif Ghulamahdi Musawira N, Salwa Zahra Nampiah Sukarno Nani Heryani Naufal, Ahmad Himi NISA RACHMANIA MUBARIK Novalita Novalita Nugroho, Mulyanto Nugroho, Robertus Nur, Hasrul Satria Nurul Hidayati Palupi, Merry Fridha Tri Prihadi, Kususanto Ditto Priyo Sadono , Teguh Putri Nur Azizah Hutagalung Qurrota A`yun, Hajar Rani Apriyani Raharja Ratnadewi, Yuliana Maria Diah Rizka Rizka Robertus Nugroho Rosyidah, Risvi Uly Sadono , Teguh Priyo Sigit Tri Wibowo Sindiya, Vita Sri Wilarso Budi Suharsono Suharsono Sukmarayu P Gedoan Sukmarayu P. Gedoan Sulistyaningsih, Yohana C Syaiful Mustofa Syuhadak, Syuhadak Tri Muji Ermayanti Tri Muji Ermayanti Turhadi Turhadi Utut Widyastuti UTUT WIDYASTUTI UTUT WIDYASTUTI W, Vika Ramadhina Wirantari, Ayu Puspa Yadi Setiadi Yanuar, Ai Ika Ayu Yohana Caecilia Sulistyaningsih Yuli Siti Fatma YULIN LESTARI Zeid B. Smeer