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All Journal Jurnal Ilmu Pertanian Bionatura Jurnal Natur Indonesia Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter Peronema Forestry Science Journal AGROEKOTEKNOLOGI Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Ilmu-Ilmu Pertanian Indonesia Dental Journal (Majalah Kedokteran Gigi) Caraka Tani: Journal of Sustainable Agriculture Abdimas Talenta : Jurnal Pengabdian Kepada Masyarakat Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Jurnal Agrotek Tropika jurnal Ilmu Pertanian (Agricultural Science) JCRS (Journal of Community Research and Service) Jurnal Agrotek Lestari PERTANIAN TROPIK CONSEN: Indonesian Journal of Community Services and Engagement Jurnal Natural
Diana Sofia Hanafiah
Department Of Agrotechnology, Faculty Of Agriculture, Universitas Sumatera Utara Jl. Dr. A. Sofian No.3, Padang Bulan, Kec. Medan Baru, Kota Medan, North Sumatera 20155, Indonesia
Religion Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Dentistry Earth & Planetary Sciences Education Energy Engineering Environmental Science Immunology & microbiology Law, Crime, Criminology & Criminal Justice Neuroscience Physics Social Sciences Other

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Journal : Jurnal Agrotek Lestari

 1 
UJI POLIMORPIK DAN HETEROZIGOSITAS PADA PROGENI F4 KEDELAI (Glycine max (L.) Merril) TAHAN SALIN DENGAN MENGGUNAKAN MARKA SSR (Simple Sequence Repeats) Evi Julianita Harahap; Rosmayanti Rosmayanti; Diana Sofia Hanafiah
Jurnal Agrotek Lestari Vol 5, No 2 (2019): Oktober
Publisher : Universitas Teuku Umar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (324.409 KB) | DOI: 10.35308/jal.v5i2.2229

Abstract

SSR marker has some merits such as quickness, simplicity, rich polymorphism and stability, thus being widely applied in genetic diversity analysis, molecular map construction and gene mapping. the purpose of this study was to determine polymorpic test and heterozygosity in F4 soybean (Glycine max (L.) Merril) progeni saline resistant characters using SSR (Simple Sequence Repeats) markers. This research was conducted in Biomolecular Laboratory, Socfindo Seed Production Laboratory (SSPL), Kebun Bangun Bandar Village Martebing District Dolok Masihul Regency Serdang Bedagai on December-May 2017. The number of samples were used 44. The five SSR primers (QS080465, QS1101, QS1112, QS100011, and Sat_091) used were specific primers, with a band pattern that was clearly visible around one or two bands. The percentage of polymorphic primers (PLP) of these three populations is high, all populations with a PLP of 100% of the saline resistant character. The effective allele number (Ne) of  7,160 for the progeny population is lower than the number of observed alleles (Na) of 10,000 which means that many progeny individuals are homozygous. The expected heterozygosity (He) value of 0.854 in the progeny population was higher than the observed heterozygosity (Ho) value of 0.027. The overall average observed heterozygosity (Ho) was 0.009 lower than the overall expected heterozygosity (He) of 0.61. This means that each character has a low heterozygosity.Keywords: Progeny F4, soybean, SSR, saline resistant, polymorphic, heterozygosity
Co-Authors Agus Pernando Simanjuntak Amaluddin Syahputra Ameilia Zuliyanti Siregar Andre Leo H Saragih Andreas Hutasoit Anna Eka ardy rahmadhani Arrasyid, Nurfida Khairina ASMARLAILI SAHAR Aznawi, Andi Aznan Chairani Hanum Chairani Hanum Christy, Julieta Dede Suhendra Dedy S Silaban Deliana Delvian Delvian Denny Satria, Denny Desta Wirnas DIAH PUSPITA ANWAR Dini Rizkita Pulungan Dohude, Gostry Aldica Dunan Naibaho E. Harso Kardhinata Edison Purba Edison Purba Elfiati, Deni Elfiati Ella Yula Ella Wati Simamora Emmy Harso Kardhinata Emmy Harso Kardhinata Emmy Harso Kardhinata etna adriana silaban etti sartina siregar Eva Sartini Bayu Evan Satria Saragih Evi Julianita Harahap Frans R Sipayung Gali Rakasiwi gredo oktavianus mario purba Guswinda N Sitanggang hani maura tiarli Harahap, Nurul Izzatunna Jhirah Hasanuddin Hasanuddin Heri Arifianto Hot Setiado Irda Safni Irfansyah Irfan irvan maulana amry Irwan Bonar Sibarani Isman Nuriadi Iwan RISNASARI Josua Simanjuntak Jumilawaty, Erni Jumilawaty Kiking Ritarwan Koko Tampubolon Lahay, Ratna Rosanty Larosa Harahap Lidya Sundari Lollie Agustina P Putri Lollie Agustina P. Putri Lollie Agustina P. Putri lubis, khairunnisa Luta, Devi Andriani Luthfi A. M. Siregar Luthfi A.M Siregar Luthfi Aziz Mahmud Siregar Mariati Sinuraya Mbue Kata Bangun Meylin Kristina Saragih Mohammad Basyuni Mubaraq, Alfian Mubaraq Muhdi Muhdi Muhdi Muhdi Muhdi Muhdi Muhdi Mutia Rahmah Nauly, Meutia Nauly Nazriah pratiwi Nisma Khairani Harahap Nurul Ain Lubis Nurul Hidayah Sinaga Olivia Avriyanti Hanafiah Onrizal Onrizal Pohan, Riko Andika Putri, Maharani Syahnia Rahmi Ami Rangkuti, Ahmad Baiquni Revandy Iskandar Muda Damanik Ria Arta Junistia Ridwanti, Batubara Risma Damayanti Risnasari, Iwan Risnasari Rohani Ganie, Rohani Ganie Rosmayanti Rosmayanti Rosmayati Rosmayati Rosmayati Rosmayati Rosmayati Rosmayati Salman Alfarisi Simangunsong Samosir, Zurni Zahara Samsuri Sartika EC Siallagan Siti Rachma Aulia Damanik Sivaipram, Itchika Sivaipram Slamet, Bejo Slamet Sri Yunita Simanjuntak Sudirman Yahya Sudirman Yahya Sudirman Yahya - Susilowati, Arida Susilowati Syaflida, Rahmi T Sabrina T. Chairun Nisa T. Sabrina Trikoesoemaningtyas ungu, retno kencana Y Husni Yoke Blandina Larasati Sihombing