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Bacteriocin Activity of Lactic Acid Bacteria from Giant Prawn (Macrobrachium rosenbergii) Meysari, Della; Helmi, Henny; Lingga, Rahmad
Al-Kauniyah: Jurnal Biologi Vol. 18 No. 1 (2025): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v1i1.38124

Abstract

AbstractFood is one of the necessities of life. Food is often added with preservatives such as chemicals that harm human health. One of the safe natural preservatives is bacteriocin compounds. Bacteriocins can be produced by lactic acid bacteria (LAB). These bacteriocins have known as Generally Recognized as Safe (GRAS) status. This study aimed to isolate and identify BAL from the digestive tract of giant shrimp (Macrobrachium rosenbergii), as well as test the ability of the bacteriocin produced to the proteolytic enzyme, temperature, pH, and salt. The research methods used were bacterial isolation, bacterial characterization, hemolysis test, bacteriocin antibacterial activity tests, proteolytic enzyme influence tests on bacteriocin activity, temperature, pH, and salt content tests on bacteriocin activity, and antibiotic tests. The research results showed that there were 37 LAB isolates and there were 7 isolates that produced bacteriocins. The LAB isolated from the digestive tract of giant prawns is Gram-positive bacteria in the form of bacilli, catalase-negative, gamma hemolytic, methyl red positive, and homofermentative. The bacteriocins can inhibit the pathogenic bacteria Staphylococcus aureus and Escherichia coli and be degraded by the Protease-K enzyme. Moreover, the bacteriocins have the characteristics of being stable at acid to neutral pH (pH 2–7), stable at low and high temperatures (4–100 °C), and stable under conditions with a salt content of 2–6.5%. The results of the identification of LAB belonged to the Lactobacillus genus.AbstrakMakanan merupakan kebutuhan pokok dalam kehidupan sehari-hari manusia. Makanan sering kali ditambahkan bahan pengawet seperti bahan kimia yang berpengaruh buruk terhadap kesehatan manusia. Salah satu alternatif bahan pengawet alami yang aman bagi kesehatan manusia adalah senyawa bakteriosin. Bakteriosin dapat dihasilkan dari bakteri asam laktat (BAL). Bakteriosin yang diproduksi oleh BAL sudah berstatus Generally Recognized as Safe (GRAS). Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi BAL dari saluran pencernaan udang galah (Macrobrachium rosenbergii), serta menguji kemampuan bakteriosin yang dihasilkan terhadap enzim proteolitik, suhu, pH dan kadar garam. Metode penelitian yang dilakukan adalah isolasi bakteri, karakterisasi bakteri, uji hemolisis, uji aktivitas antibakteri bakteriosin, uji pengaruh enzim proteolitik, suhu, pH dan kadar garam terhadap aktivitas bakteriosin. Hasil isolasi terdapat 37 isolat BAL dan 7 isolat yang menghasilkan bakteriosin. BAL yang diisolasi dari saluran pencernaan udang galah merupakan bakteri Gram positif berbentuk basil, katalase negatif, gamma hemolisis, methyl red positif dan homofermentatif. Bakteriosin mampu menghambat bakteri patogen Staphylococcus aureus dan Escherichia coli, dapat didegradasi oleh enzim Protease-K, stabil pada pH asam hingga netral (pH 2–7), stabil pada suhu rendah maupun tinggi (4°–100 °C) dan stabil pada kondisi dengan kadar garam 2–6,5%.  Hasil identifikasi BAL dari usus udang galah yaitu bakteri termasuk dalam Genus Lactobacillus.
THE INFLUENCE OF THE EDUCATION OF THE TA'LIMUL QUR'AN ASSEMBLY IN THE FORMATION OF CHILDREN'S CHARACTER IN SENURO VILLAGE Jannah, Roudhotul; Helmi, Henny; Husain, Azizah
Al-Mubin Jurnal Ilmiah Islam
Publisher : Department of Research and Community Service at the Ummul Quro Al-Islami Bogor Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51192/almubin.v8i2.1757

Abstract

This research aims to examine the influence of education at Majelis Ta'limul Qur'an on the character building of children in Senuro Village, with a special focus on the learning materials delivered in instilling moral and religious values in children. This research uses a descriptive qualitative approach, using purvosiv random samlping techniques, with data collection techniques in the form of interviews, observation, and documentation. The results of the study show that the learning materials at Majelis Ta'limul Qur'an are delivered through interactive methods and in accordance with the development of children's age, such as dialog, storytelling, exemplary, targhib and tarhib and habituation. The material effectively instills character values in children. Children who participated in the assembly activities experienced an increase in positive attitudes, such as discipline, better morals, and increased responsibility. Parents also feel the positive impact of the learning materials provided, seen from changes in children's behavior at home and the surrounding environment. Thus, education at Majelis Ta'limul Qur'an not only improves children's religious abilities but also becomes a fortress in shaping character in accordance with Islamic values amid the challenges of the times
Identifkasi Bakteri dan Fungi Udara pada Pusat Perbelanjaan di Pangkal Pinang Helmi, Henny; Vebriani, Helly; Juliani, Intan; Karina, Karina; Junita, Junita
EKOTONIA: Jurnal Penelitian Biologi, Botani, Zoologi, dan Mikrobiologi Vol 8 No 1 (2023): Ekotonia: Jurnal Penelitian Biologi, Botani, Zoologi dan Mikrobiologi
Publisher : Department of Biology, Faculty of Agriculture, Fisheries and Biology, University of Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/ekotonia.v8i1.4110

Abstract

Shopping center is one of the densest areas of human activity, so that the gathering of humans can cause transmission of microorganisms that cause air borne disease. This research was conducted to find out the various types of air pollutant bacteria and fungi on the 2nd Floor of One of the Shopping Center Areas in Pangkal Pinang City. This research was conducted experimentally using Nutrien Agar and Potato Dextrose Agar media for enumeration of bacteria and fungi. Identification of bacteria and fungi was carried out macroscopically, microscopically and biochemically. The results of this study showed that there were 9 bacterial isolates and 4 fungal isolates. The genus of bacteria found is Bacillus sp. Staphylococcus sp. and Nitrobacter sp. As for the genus of fungi found, among others, namely Aspergillus sp. The total bacteria and fungi present in the air in the shopping center have not exceeded the quality standards that have been set, so that it can be said that the air in one of the Pangkal Pinang City Shopping Centers is still healthy