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All Journal HAYATI Journal of Biosciences JURNAL KIMIA SAINS DAN APLIKASI Jurnal Penelitian Sains (JPS) Microbiology Indonesia SEMIRATA 2015 CHEMPUBLISH JOURNAL Jurnal Penelitian Sains (JPS) IJFAC (Indonesian Journal of Fundamental and Applied Chemistry) Science and Technology Indonesia Jurnal Penelitian Sains Martabe : Jurnal Pengabdian Kepada Masyarakat JSFK (Jurnal Sains Farmasi & Klinis) Sainmatika: Jurnal Ilmiah Matematika dan Ilmu Pengetahuan Alam Molekul: Jurnal Ilmiah Kimia JPPM (Jurnal Pengabdian dan Pemberdayaan Masyarakat) Jurnal Abdi Insani Jurnal Pengabdian Masyarakat: Humanity and Medicine Jurnal Pengabdian Masyarakat Indonesia SRIWIJAYA JOURNAL OF ENVIRONMENT BIOVALENTIA: Biological Research Journal Joong-Ki : Jurnal Pengabdian Masyarakat Jurnal Pepadu Karunia: Jurnal Hasil Pengabdian Masyarakat Indonesia Joong-Ki Joong-Ki Analit: Analytical And Environmental Chemistry
Heni Yohandini
Department Of Chemistry, Faculty Of Mathematics And Natural Sciences, University Of Sriwijaya, Inderalaya, Ogan Ilir, South Sumatra, 30662 Indonesia
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Journal : BIOVALENTIA: Biological Research Journal

 1 
CONDITION OPTIMIZATION PRODUCTION XYLANASE THERMOSTABLE BY Bacillus licheniformis TS10 USING SUBSTRATE OIL PALM EMPTY FRUIT BUNCHES Safitri, Ayu; Muharni, -; Kusumawati, Heni Yohandini
BIOVALENTIA: Biological Research Journal Vol. 3 No. 1 (2017)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (133.065 KB) | DOI: 10.24233/BIOV.3.1.2017.51

Abstract

Optimization conditions for thermostable xylanase production of Bacillus licheniformis TS10 by using substrate oil palm empty fruit bunches  has been conducted from November 2015 to January 2016 in the Laboratory of Genetics and Biotechnology, Department of Biology, Faculty of Mathematics and Natural Sciences, University of Sriwijaya, Indralaya. The aims of this study was to determine the potential of oil palm empty fruit bunches ( EFB ) as a substrate for the production of thermostable xylanase by Bacillus licheniformis TS10 and the optimum conditions of temperature, pH and substrate concentration in the fermentation process. The research method is to make the growth curve and the curve of Bacillus licheniformis TS10 enzyme production on EFB substrate. The number of bacterial cells was determined by using a UV-VIS spectrophotometer and total plate count (TPC) through a standard curve. Optimization performed at various pH (5, 6, 7, 8, 9), temperature (50 °C, 60 °C, 70 °C, 80 °C) and substrate (1%, 2%, 3%, 4%) , Test activity at each pH, temperature and substrate using methods DNS by measuring enzyme activity based on the reducing sugar released by the substrate by using dinitrosalicylic acid (DNS). Based on the results of this research showed that oil palm empty fruit bunches (EFB ) has potential as a substrate for the production of thermostable xylanase by Bacillus licheniformis TS10, thermostable xylanase production of Bacillus licheniformis TS10 on the substrate EFB has an optimum condition at pH 6, 80 °C and the substrate concentration of 4%.
CONDITION OPTIMIZATION PRODUCTION XYLANASE THERMOSTABLE BY Bacillus licheniformis TS10 USING SUBSTRATE OIL PALM EMPTY FRUIT BUNCHES Safitri, Ayu; Muharni, -; Kusumawati, Heni Yohandini
BIOVALENTIA: Biological Research Journal Vol. 3 No. 1 (2017)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (133.065 KB) | DOI: 10.24233/BIOV.3.1.2017.51

Abstract

Optimization conditions for thermostable xylanase production of Bacillus licheniformis TS10 by using substrate oil palm empty fruit bunches  has been conducted from November 2015 to January 2016 in the Laboratory of Genetics and Biotechnology, Department of Biology, Faculty of Mathematics and Natural Sciences, University of Sriwijaya, Indralaya. The aims of this study was to determine the potential of oil palm empty fruit bunches ( EFB ) as a substrate for the production of thermostable xylanase by Bacillus licheniformis TS10 and the optimum conditions of temperature, pH and substrate concentration in the fermentation process. The research method is to make the growth curve and the curve of Bacillus licheniformis TS10 enzyme production on EFB substrate. The number of bacterial cells was determined by using a UV-VIS spectrophotometer and total plate count (TPC) through a standard curve. Optimization performed at various pH (5, 6, 7, 8, 9), temperature (50 °C, 60 °C, 70 °C, 80 °C) and substrate (1%, 2%, 3%, 4%) , Test activity at each pH, temperature and substrate using methods DNS by measuring enzyme activity based on the reducing sugar released by the substrate by using dinitrosalicylic acid (DNS). Based on the results of this research showed that oil palm empty fruit bunches (EFB ) has potential as a substrate for the production of thermostable xylanase by Bacillus licheniformis TS10, thermostable xylanase production of Bacillus licheniformis TS10 on the substrate EFB has an optimum condition at pH 6, 80 °C and the substrate concentration of 4%.
BIODECOLORIZATION OF TEXTILE INDUSTRIAL WASTE BY THERMOPHILIC BACTERIA Anoxybacillus rupiensis TS04 AND Anoxybacillus flavithermus TS15 Muharni, Muharni; Yohandini, Heni; Rivai, M Yunus
BIOVALENTIA: Biological Research Journal Vol. 4 No. 1 (2018)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (351.91 KB) | DOI: 10.24233/BIOV.4.1.2018.96

Abstract

Wastewater of textile industries contained a high content of synthetic dyes that could damage the aquatic ecosystem and environmental aesthetics. The use of microorganisms on the biodecolorization of textile industrial waste had advantages such as low cost and environmentally friendly. The purpose of the study was to determine biodecolorization capability of Anoxybacillus rupiensis TS04 and Anoxybacillus flavithermus TS15 for industrial waste of tie-dye fabrics. Completely randomized design with factorial pattern was used in the research; factor I and II were concentrations of wastewater and types of bacteria, respectively Anoxybacillus rupiensis TS04 showed the highest decolorization ability by 83.25% for wastewater concentration of 80% (v/v) and Anoxybacillus flavithermus TS15 by 69% at 40% (v/v) waste concentration.The highest cell number of Anoxybacillus rupiensis TS04 was obtained as 1.52 x 106 cfu/mL and biodecolorization, textile industrial waste, thermophilic bacteria,Anoxybacillus flavithermus TS15 3.70 x 105cfu/mL.
Co-Authors . AKHMALOKA Abd. Rasyid Syamsuri Ady Mara, Ady Afreni Hamidah Alfarado, Daniel Alfian Hasbi Almunady T. Panagan Almunady T. Panagan Ani Sarah Arini, Feggy Augesti Erisna Ayu Lestari Ayu Safitri Bella Safitri Bijak Riyandi Ahadito Budiono Budiono Carissa, Restri Diah Cenora, Cindy Cindy Cenora Desnelli Desnelli Dirli Fahmi Rizal Elfita Elfita Elfita Elfita Elfita Elfita Elfita Elfita Elfita Elfita Elfita Elfita Elfita Elisa Nurnawati Eliza Eliza Eliza Eliza Eliza Eliza Elvi Elvi Esthy Rahman Asih Evi Kaderani Barutu Fatma Feggy Arini Ferlinahayati Ferlinahayati FIDA MADAYANTI Fitrya . Fitrya Fitrya Fitrya Fitrya Fitrya Fitrya Fitrya Fitrya Ghaniya, Fahdelaa Hadir Kaban, Hadir Hary Widjajanti Hary Widjajanti Hermansyah Hermansyah Hermansyah Hermansyah Herwin Purnomo IHSAN IVANTRI Isrowiyatun Daiyah Jasmi Jasmi Jojor Uli Gultom Jorena Jorena, Jorena Julinar Julinar Julinar Julinar Julinar Julinar Julinar Juraida Roito Hrp Jurnal Pepadu Juswardi Juswardi Lily Restusari Maghfirahmah Amsyah Putri Marieska Verawaty Maryadi Maryadi Maryadi Maryadi Melinda Susanti S Melly Wardanis Melsa Nilmalasari Miksusanti Miksusanti Mila Wulandari Mila Wulandari Mita Puspitasari Mudinillah, Adam MUHAMMAD SAID Muharni Muharni Muharni Muharni Muharni Muharni Muharni Muharni Muharni Muharni Muharni Muharni Muharni Muharni Muharni, . Munawar Munawar Nirwan Syarif Nirwan Syarif, Nirwan Nita Aminasih Nova Yuliasari oktaviani, muthia PINGKAN ADITIAWATI Poedji Loekitowati Hariani Poedji Loekitowati Hariani Pramoe Wibowo Putra Setia Aji Candra Rahmi Junaida Rifdah Nabila Riska Adillah Rivai, M Yunus Rivai, M Yunus Rizkiani, Delisa Rr. Kusuma Nurin Husna Safitri, Bella Salni Salni Seni Metasari Seni Metasari Stephani Stephani Suheyanto Suheryanto Susilawati Susilawati Susilawati, Susilawati Syafrina Lamin, Syafrina Yeni Aryani Yulia Fitri Yundari, Yundari