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Uji Toksisitas Ekstrak n-Heksana, Etil Asetat, dan Metanol Daun Bintaro (Cerbera odollam G) terhadap Artemia salina Leach Tanti Nurani; Asep Supriadin; Adisty Virakawugi Darniwa
Gunung Djati Conference Series Vol. 44 (2024): Seminar Nasional (SEMNAS) Kimia Tahun 2024
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Kanker merupakan ancaman serius bagi manusia karena sel-sel tubuh yang tumbuh secara abnormal menyerang organ tertentu dan berkembang biak dengan cepat, merusak sel-sel tubuh. Tanaman bintaro (Cerbera Odollam G) diketahui mengandung senyawa metabolit sekunder seperti alkaloid, terpenoid, dan tanin, yang dilaporkan memiliki sifat antimikroba, analgesik dan menunjukkan aktivitas sitotoksik terhadap berbagai jenis kanker. Penelitian ini bertujuan untuk mengidentifikasi senyawa mrtabolit sekunder yang terkandung pada ekstrak n-heksana, etil asetat, dan metanol daun bintaro (Cerbera Odollam G) serta mengetahui tingkat toksisitas ekstrak ekstrak n-heksana, etil asetat, dan metanol daun bintaro (Cerbera Odollam G) terhadap larva Artemia salina L dengan metode Brine Shrimp Lethality Test (BSLT) dengan nilai LC50. Penelitian ini menggunakan 450 ekor larva udang (Artemia salina L) yang terbagi menjadi tiga kelompok ekstrak, lima konsentrasi yaitu 10, 15, 20, 25, dan 30 ppm serta tiga kali pengulangan dengan perlakuan masing-masing 10 ekor larva udang. Hasil penelitian menunjukan bahwa ekstrak n-heksana, etil asetat dan metanol daun bintaro menunjukan positif mengandung senyawa alkaloid, flavonoid, saponin, tanin, dan steroid. Hasil mortalitas pada larva didapatkan nilai probit untuk mengetahui nilai LC50 yang ditunjukan pada ekstrak n-heksana sebesar 1,00033 ppm sedangkan pada ekstrak etil asetat dan metanol adalah sebesar 40,66053 ppm dan 74,15075 ppm yang menunjukan ekstrak daun bintaro berpotensi sebagai antikanker.
Analysis of Essential Oil Compounds from Eucalyptus (Eucalyptus pellita) Leaves and Their Bioactivity Against Staphylococcus aureus Alam, Rizki Nur; Supriadin, Asep; Kurniasih, Nunung
Walisongo Journal of Chemistry Vol. 7 No. 2 (2024): Walisongo Journal of Chemistry
Publisher : Department of Chemistry Faculty of Science and Technology Walisongo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/wjc.v7i2.21603

Abstract

Eucalyptus (Eucalyptus pellita) is a plant commonly found in Garut Regency, Indonesia, with its leaves showing potential for essential oil extraction. Hence, this study aimed to determine the yield, analyze the chemical composition, and evaluate the bioactivity of essential oils derived from Eucalyptus pellita leaves against Staphylococcus aureus. The essential oil was extracted utilizing Stahl distillation from leaves collected in Cisarua Village, Samarang District, Garut Regency. The chemical composition was identified through Gas Chromatography-Mass Spectrometry (GC-MS), and its antibacterial activity against S. aureus was assessed using the disc diffusion method. As a result, the average essential oil yield was 0.34%, with compounds identified as α-phellandrene (3.20%), α-terpineol (5.52%), γ-terpinene (7.07%), 1,8-cineole (7.26%), α-terpinyl acetate (13.40%), and limonene (50.47%). The bioactivity test results indicated that the inhibition zone diameter at a 25% concentration of essential oil was categorized as moderate, while concentrations of 50% and 100% demonstrated strong antibacterial activity.
Analysis of Essential Oil Compounds from Eucalyptus (Eucalyptus pellita) Leaves and Their Bioactivity Against Staphylococcus aureus Alam, Rizki Nur; Supriadin, Asep; Kurniasih, Nunung
Walisongo Journal of Chemistry Vol. 7 No. 2 (2024): Walisongo Journal of Chemistry
Publisher : Department of Chemistry Faculty of Science and Technology UIN Walisongo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/wjc.v7i2.21603

Abstract

Eucalyptus (Eucalyptus pellita) is a plant commonly found in Garut Regency, Indonesia, with its leaves showing potential for essential oil extraction. Hence, this study aimed to determine the yield, analyze the chemical composition, and evaluate the bioactivity of essential oils derived from Eucalyptus pellita leaves against Staphylococcus aureus. The essential oil was extracted utilizing Stahl distillation from leaves collected in Cisarua Village, Samarang District, Garut Regency. The chemical composition was identified through Gas Chromatography-Mass Spectrometry (GC-MS), and its antibacterial activity against S. aureus was assessed using the disc diffusion method. As a result, the average essential oil yield was 0.34%, with compounds identified as α-phellandrene (3.20%), α-terpineol (5.52%), γ-terpinene (7.07%), 1,8-cineole (7.26%), α-terpinyl acetate (13.40%), and limonene (50.47%). The bioactivity test results indicated that the inhibition zone diameter at a 25% concentration of essential oil was categorized as moderate, while concentrations of 50% and 100% demonstrated strong antibacterial activity.
Product awareness and learning motivation on plantae topics through project-based learning with halal literacy Firdaus, Muhammad Taufiq; Wulandari, Ismi Ayu; Windayani, Neneng; Supriadin, Asep
JPBIO (Jurnal Pendidikan Biologi) Vol 9, No 2 (2024): November 2024
Publisher : STKIP Persada Khatulistiwa Sintang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31932/jpbio.v9i2.4008

Abstract

In the ever-evolving world of education, an in-depth understanding of the learning process is essential. Lecture methods that tend to be monotonous often reduce students' motivation to learn, which in turn has an impact on decreasing learning outcomes, especially in plant material. Project-based learning has emerged as an alternative to increase students' interest in learning. This study aims to instill halal literacy awareness while influencing interest in purchasing halal products through a project-based learning approach on plantae material. A quantitative approach with a targeted survey design was used in this study. Thus, we used the purposive sampling technique to define 28 students as respondents. Data were collected through a questionnaire with a Likert scale (1-4). The analysis results show that the student awareness variable (X1) has a negative correlation with halal literacy project-based learning. Through multiple linear regression analysis, no symptoms of multicollinearity or heteroscedasticity were found. Halal literacy project-based learning contributed 30.8% to increasing students' awareness and interest in purchasing halal products. Thus, project-based learning is proven effective in increasing students' interest in learning, especially in plantae material.