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All Journal Jurnal Hortikultura Indonesia (JHI) Jurnal Agroteknotropika Berkala Ilmiah Pertanian Jurnal Produksi Tanaman Jurnal Penelitian Pertanian Terapan JPT : JURNAL PROTEKSI TANAMAN (JOURNAL OF PLANT PROTECTION) Abditani : Jurnal Pengabdian Masyarakat Madaniya Bioscientist : Jurnal Ilmiah Biologi BIOVALENTIA: Biological Research Journal Jurnal Pengabdian Masyarakat Teknologi Pertanian Jurnal lahan Pertanian Tropis Al-Hayat: Natural Sciences, Health & Environment Journal International Journal of Technology and Education Research
Indriati Husain
Universitas Negeri Gorontalo
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences Other

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Genetic Variation of Sorghum (Sorghum bicolor L.) at Several Colchicine Concentrations Based on Agronomic Characteristics Adam, Marta; Husain, Indriati; Yamin, Mayasari; Nurdin; Dude, Suyono
International Journal of Technology and Education Research Vol. 3 No. 04 (2025): October - December, International Journal of Technology and Education Research
Publisher : International journal of technology and education research

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.63922/ijeter.v3i04.2523

Abstract

Sorghum (Sorghum bicolor L.) serves as an alternative food source to rice due to its rich nutritional content. The development of sorghum through breeding can be facilitated using colchicine. This study aimed to: (1) identify sorghum varieties exhibiting the best performance based on agronomic traits; (2) determine the effective colchicine treatment for increasing genetic variation in sorghum varieties; (3) identify agronomic traits with high genetic variability influenced by multiple genes; and (4) evaluate the best interaction between colchicine and variety based on sorghum agronomic traits. The study was conducted from January to April 2025 in the Greenhouse of the Agricultural Assembly and Modernization Agency, Gorontalo. The experiment employed a factorial Completely Randomized Design (CRD), where the first factor consisted of two varieties, Numbu and Kawali, and the second factor comprised four colchicine concentrations: 0 ppm, 750 ppm, 1000 ppm, and 1250 ppm. Each treatment was replicated three times, resulting in a total of 24 experimental units. The results indicated that the Numbu variety exhibited the highest genetic variability based on leaf area; a colchicine concentration of 750 ppm effectively enhanced sorghum plant variability; plant height, panicle length, and leaf area displayed high genetic variation and were controlled by multiple genes; and the interaction of the Numbu variety with 750 ppm colchicine showed the most favorable performance based on the selection traits of panicle length, plant height, and leaf area. Therefore, this combination holds potential for the colchicine-based development of sorghum
Induction Of In Plantain (Musa acuminata L.) Using The Growth Regulators 2,4-Dichlorophenoxyacetic & BAP (Benzyl Amino Purin) Nur Fitriani, Defy; Indriati Husain; Sutrisno Hadi Purnomo
International Journal of Technology and Education Research Vol. 3 No. 04 (2025): October - December, International Journal of Technology and Education Research
Publisher : International journal of technology and education research

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.63922/ijeter.v3i04.2608

Abstract

Plat propagation through is a method of prodycing new plants by growing unspecified clusters (callus) from small pieces of tissue (explants) such as Gapi banana flowers. This study seeks to determine the appropriate doses of 2,4-D and BAP for callus formation. The study was conducted from September to October 2025 at the Tissue Culture Laboratory of Gorontalo State University, using male Gapi banana flowers explants. The experiment used a randomized block design (RBD) involving two factors: 2,4-D (2ppm) and BAP (5, 10, 15 ppm). The percentage of live explants (%) was observed weekly for 30 days. Final observation included browning, The results showed that the addition of 2,4-D and BAP to the medium greatly affected the percentage of live explants. Without these substances, the explants turned brown. Fungal contamination was dominant at D0 (control) and D2 (2 ppm 2,4-D + 10 ppm BAP). The callus was white/cream to greenish in color, with a firm texture. The percentage of callus-forming explants (%) increased with the addition of BAP (5, 10 and 15ppm), significantly affecting callus formation.
Co-Authors Adam, Marta Adju, Fitria Y.Hs. Agus Purwito Ali Husni Angry Pratama Solihin Antuli, Zainuddin Dama, Hasna Devi Bunga Pagalla Devi Bunga Pagalla Dude, Suyono Eyato, Rika Fauzan Zakaria Fitriah S. Jamin Hasan, Erfina N Hasna Dama Hasna Dama Isima , Lindawaty Ismail, Fatmawati Jusna Ahmad Kadek Setiawati Kikin H Mutaqin Lamante, Nazwa S. Lindawaty Isima Marleni Limonu Mayasari Yamin, Mayasari Miftahul Fikriyah Adudu Muhammad Arief Azis Ngadi, Suleman Novri Youla Kandowangko Nur Fitriani, Defy Nurdin . Nurmi Rahman Yusuf, Abd Rully Tuiyo Saleh, Risky Yanto Silvana Apriliani Silviana Arsyad Silviana Arsyad Slamet Susanto Surdaya, Tedy Sutrisno Hadi Purnomo Sutrisno Hadi Purnomo Suwatan, Silvany Syamsul Bahri Wawan Pembengo Yunnita Rahim