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Test of Bamboo Leaf Bacterial Potential on the Growth and Yield of Shallot Plants (Allium ascalonicum L.) Sulle, Atrieda; Husain, Indriati; Irhamnawati Pulogu, Siska; Zakaria, Fauzan; Yamin, Mayasari
International Journal of Technology and Education Research Vol. 4 No. 01 (2026): January- March, International Journal of Technology and Education Research (IJ
Publisher : International journal of technology and education research

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.63922/ijeter.v4i01.2766

Abstract

This research was carried out at the Laboratory of the Department of Agrotechnology, Faculty of Agriculture, Gorontalo State University and at the Green House of the Gorontalo Agricultural Assembly and Modernization Agency from December 2024 to April 2025. This study aims to determine the effect of soaking onion bulbs with bamboo leaf bacterial isolate on the growth and yield of shallot plants and to obtain bamboo leaf bacterial isolate that has the potential to affect the growth and yield of shallot plants. The study was designed using a Random Design Group (RDG) with a suspension treatment of bamboo leaf bacterial isolates consisting of seven types of bacterial isolates that have not yet been identified, namely, X1, X2, X3, X4, X5, X6, X7, and X0 as controls (without bacterial suspension). The parameters measured were plant height, number of leaves, number of seedlings, number of bulbs, weight of wet bulbs, and weight of dry bulbs. The observation data was analyzed using Analysis of Variance (ANOVA α = 5%), and if the results were significantly different, DMRT was further tested at the level of 5%. The results showed that the X2 bacterial isolate suspension treatment had a real effect on the number of seedlings, and had a good influence on the parameters of the number of bulbs and the weight of dry bulbs.
Pembentukan Populasi Awal Mutan M0 Gandum (Triticum Aestivum L.) Menggunakan Mutagen Kolkisin Mayasari Yamin; Indriati Husain; Silviana Arsyad; Hasna Dama; Taufiq Hidayat RS; Andini Citra Amalia; Soraya Wartabone
Jurnal Galung Tropika Vol 15 No 1 (2026)
Publisher : Fakultas Pertanian, Peternakan dan Perikanan Universitas Muhammadiyah Parepare

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31850/jgt.v15i1.1458

Abstract

Wheat imports are increasing due to the demand for wheat consumption, especially processed wheat, but not balanced by the production of wheat seed raw materials. To overcome this problem, it is necessary to assemble New Superior Varieties of wheat that are adaptive to Indonesian environmental conditions. This VUB assembly begins with the formation of an initial population conventionally, one of which can be through the use of chemical mutagens, namely colchicine. This study aims to obtain an effective colchicine concentration in increasing genetic variation, increasing genetic variation in the initial population of M0 wheat mutants, obtaining morphophysiological characters that have high coefficients of diversity, and obtaining M1 wheat seeds as candidates for the next generation. This research method begins with the selection stage of wheat parent seeds from several wheat varieties based on the physical aspects of the seeds and the characteristics of the yield components, soaking the sprouts using a colchicine mutagen solution, and planting the sprouts from the selected wheat parent seeds using a randomized block design consisting of five colchicine mutagen soaking treatments, namely without soaking; 400 ppm colchicine soaking; 600 ppm colchicine soaking; 800 ppm colchicine soaking; and 1000 ppm colchicine soaking. Each treatment was repeated three times. The results showed that 400 ppm of colchicine mutagen was an effective and dominant concentration for increasing genetic variation based on growth percentage, plant height, leaf number, and leaf length. The use of colchicine mutagens increased genetic variation due to its polyploidy properties, which are determined by the number of stomata formed.
Evaluasi Kesesuaian Lahan untuk Tanaman Cabai Merah (Capsicum annuum L.) di Kecamatan Tabongo Kabupaten Gorontalo Arifka Mutmainah; Muhammad Arief Azis; Indriati Husain
Research Review: Jurnal Ilmiah Multidisiplin Vol. 4 No. 1 (2025): Research Review: Jurnal Ilmiah Multidisiplin (Februari 2025 - Juli 2025)
Publisher : Transbahasa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54923/researchreview.v4i1.127

Abstract

In Tabongo District, the harvested area for red chili plants remained constant at 1 hectare from 2019 to 2020. However, there was a significant decline in production, as evidenced by the red chili yield, which reached 27,200 kg in 2019 but dropped drastically to only 700 kg in 2020. This study aims to (1) analyze the land characteristics and quality in Tabongo District, Gorontalo Regency, and (2) determine the land suitability class for red chili cultivation in the area. The research was conducted using a survey method and land suitability matching criteria for red chili plants. The results indicate that the land characteristics and quality in the study area generally contain adequate soil nutrients. The actual land suitability for red chili cultivation in Tabongo District is predominantly classified as S3 (marginally suitable) with an area of 1,003.44 hectares. Meanwhile, the potential land suitability is mainly classified as S2 (moderately suitable) with a total area of 954.67 hectares.
Respon Pemberian Pupuk Kandang Kambing dan Arang Sekam Terhadap Pertumbuhan dan Hasil Tanaman Bawang Merah (Allium ascalonicum L.) Varietas Tajuk Fery Firmansyah; Indriati Husain; Fauzan Zakaria
Research Review: Jurnal Ilmiah Multidisiplin Vol. 4 No. 1 (2025): Research Review: Jurnal Ilmiah Multidisiplin (Februari 2025 - Juli 2025)
Publisher : Transbahasa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54923/researchreview.v4i1.130

Abstract

Shallots (Allium ascalonicumK.) are vegetable commodities that hold significant importance for society, both in terms of their high economic value and nutritional content. This research aimed to determine the appropriate combination of goat manure and rice husk charcoal doses for the growth and yield of shallot plants. The study was conducted in Bunggalo Village, Telaga Jaya Subdistrict, Gorontalo Regency, from July to September 2024. The research employed a Completely Randomized Design (CRD) with 4 treatmens, each repeated 4 times, resulting in 16 experimental plots. Each plot contained 3 plants, resulting in total of 48 plants. P0 consisted of soil only, P1 consisted of soil, goat manure, and rice husk charcoral in a ratio of (1:2:3), P2 consisted of soil, goat manure and rice husk charcoral in a ratio of (2:1:1) and P3 consisted of soil, goat manure and rice husk charcoral in a ratio of (3:2:1). Each pot contained a total of 6kg of planting media. The observational data ware analyzed using F-test (ANOVA) at a 5% significance level. If significant effect were observed, further analysis was conducted using DMRT at a 5% level. The result indicated that P3 was the best treatment with a (3:2:1) combination for plant height and the nuraber of leaves, while P2 was the best treatment with a (2:1:1) combination for the number of bulbs, fresh weight, and dry weight of shallot plants.
Kandungan Klorofil dan Pertumbuhan Bawang Merah pada Pemberian Pupuk Organik Cair dan Benzyl Amino Purine Silvana Apriliani; Indriati Husain; Rival Rahman
AGROSCRIPT: Journal of Applied Agricultural Sciences Vol 8 No 1 (2026): June
Publisher : Universitas Perjuangan Tasikmalaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36423/agroscript.v8i1.2785

Abstract

Bawang merah merupakan komoditas hortikultura penting yang pertumbuhan dan produksinya dipengaruhi oleh ketersediaan unsur hara serta keseimbangan fisiologis tanaman. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian pupuk organik cair dan BAP terhadap kandungan klorofil dan pertumbuhan tanaman bawang merah serta untuk mengetahui interaksi keduanya dalam meningkatkan efisiensi fotosintesis dan pertumbuhan tanaman. Penelitian dilakukan di Desa Huntu Utara Kecamatan Bulango Selatan. Penelitian menggunakan Rancangan Acak Kelompok Lengkap (RAKL) dengan tiga kali ulangan. Perlakuan pertama terdiri dari empat dosis POC kulit pisang kepok (0 mL L-1, 50 ml L-1, 100 ml L-1, 150 ml L-1) dan konsentrasi BAP (0 ppm, 100 ppm dan 200 ppm). Variabel pengamatan meliputi jumlah daun, klorofil a, klorofil b, rasio klorofil a/b, total klorofil, jumlah umbi, bobot basah umbi dan bobot kering umbi. Hasil penelitian menunjukkan bahwa pemberian POC kulit pisang kepok berpengaruh nyata pada variabel bobot kering umbi, tetapi tidak berpengaruh nyata pada jumlah daun, klorofil a, klorofil b, rasio klorofil a/b, total klorofil, jumlah umbi dan bobot basah umbi. Adapun konsentrasi BAP berpengaruh nyata pada variabel jumlah umbi. Interaksi antara POC dan BAP berpengaruh tidak nyata terhadap seluruh variabel pengamatan
High Parasitism of Telenomus and Trichogramma Species on Spodoptera frugiperda Eggs During Maize Vegetative Stage in Gorontalo, Indonesia Angry Pratama Solihin; Silvany Suwatan; Indriati Husain
Jurnal Proteksi Tanaman (Journal of Plant Protection) Vol. 9 No. 1 (2025): June 2025
Publisher : Plant Protection Department, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.9.1.38-47.2025

Abstract

The fall armyworm (Spodoptera frugiperda, Lepidoptera: Noctuidae) is an invasive pest that threatens maize production in Indonesia, particularly during the vegetative stage. This study aimed to identify egg parasitoid species and assess their parasitism levels on S. frugiperda during this critical growth phase in Gorontalo, Indonesia. Field sampling was conducted from February to April 2021 using random sampling of egg masses at three maize fields in Suwawa District. The collected eggs were incubated in the laboratory to observe emergence and identify parasitoids. This study revealed the significant contribution of egg parasitoids—particularly Telenomus sp.—in suppressing S. frugiperda populations during the vegetative stage of maize in Gorontalo, Indonesia. Parasitism rates were remarkably high across all observation periods, ranging from 83.82% to 92.54%, and the emergence of Telenomus sp. consistently exceeded that of Trichogramma sp. These findings demonstrate that native egg parasitoids are already actively controlling S. frugiperda in the field and should be preserved through ecologically sensitive pest management. Avoiding insecticide use during early maize growth stages is essential to maintaining the activity of beneficial parasitoids.
Identifikasi Tahap Perkembangan Mikrospora melalui Ukuran Floret pada Jantung Pisang Tanduk Devi Bunga Pagalla; Jusna Ahmad; Indriati Husain; Nazwa S. Lamante; Rika Eyato
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 4 (2025): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i4.17681

Abstract

This study aimed to identify the relationship between floret length and the developmental stages of microspores in banana male inflorescence (Musa spp. cv. Tanduk), and to determine the optimal floret size range as an explant source for initiating microspore culture. Ten bract layers were analyzed, with five florets randomly selected from each layer and measured for their length. Anthers were extracted from each floret, crushed in sterile water, and observed microscopically using a wet mount preparation. The results revealed a strong correlation between floret length and microspore developmental stages. Microspores at the late uninucleate (vacuolated) to early bicellular stages were consistently found in florets ranging from 4.68 to 4.34 cm in length. Based on these findings, florets within the 4.60 to 4.00 cm range are recommended as the optimal size for microspore culture initiation, as they contain a higher proportion of developmentally competent microspores for embryogenesis induction. In conclusion, this study provides essential morphological and cytological criteria to support efficient explant selection for in vitro regeneration of banana through the microspore culture approach.
Respon Eksplan Tangkai Daun Dan Batang Muda Mangrove (Bruguiera gymnorrhiza L.) pada Media dengan Penambahan IAA dan Kinetin Tri Vinesa Moha; Novri Youla Kandowango; Jusna Ahmad; Febriyanti Febriyanti; Devi Bunga Pagalla; Indriati Husain
Jambura Edu Biosfer Journal Vol 7, No 1 (2025): Jambura Edu Biosfer Journal
Publisher : Fakultas MIPA Universitas Negeri Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34312/jebj.v7i1.27840

Abstract

Ekosistem mangrove memiliki peran penting dalam menjaga stabilitas lingkungan pesisir, namun keberadaannya terus mengalami tekanan akibat aktivitas manusia. Salah satu spesies mangrove yang penting untuk dikembangkan dalam upaya rehabilitasi kawasan pesisir adalah Bruguiera gymnorrhiza L. Ketersediaan bibit secara konvensional masih menjadi kendala karena membutuhkan waktu relatif lama dan sangat bergantung pada ketersediaan bahan tanam di alam. Oleh karena itu, kultur jaringan in vitro menjadi salah satu alternatif potensial untuk mendukung perbanyakan tanaman mangrove secara lebih cepat dan terkontrol. Penelitian ini bertujuan untuk mengetahui pengaruh kombinasi IAA dan kinetin terhadap induksi kalus pada eksplan tangkai daun dan batang muda mangrove Bruguiera gymnorrhiza L. secara in vitro. Penelitian ini menggunakan metode eksperimen dengan rancangan acak lengkap (RAL). Perlakuan yang digunakan terdiri atas P1, yaitu 0,25 ppm IAA + 0,25 ppm kinetin; P2, yaitu 0,5 ppm IAA + 0,5 ppm kinetin; P3, yaitu 0,75 ppm IAA + 0,75 ppm kinetin; P4, yaitu 1 ppm IAA + 1 ppm kinetin; dan P5 sebagai kontrol tanpa penambahan IAA dan kinetin. Setiap perlakuan diulang sebanyak tiga kali. Parameter yang diamati meliputi waktu muncul kalus, morfologi kalus, dan warna kalus. Data dianalisis secara deskriptif kualitatif. Hasil penelitian menunjukkan bahwa perlakuan P1 memberikan respons terbaik terhadap induksi kalus dengan waktu muncul kalus tercepat, yaitu pada 19 hari setelah tanam (HST). Kalus yang terbentuk pada perlakuan tersebut memiliki karakteristik berwarna kuning. Hasil ini menunjukkan bahwa kombinasi IAA dan kinetin pada konsentrasi rendah berpotensi mendukung induksi kalus pada eksplan tangkai daun dan batang muda Bruguiera gymnorrhiza L. secara in vitro.
Induksi Kalus dari Antera Pisang Goroho (Musa acuminata L.) dengan Menggunakan 2,4-D dan BAP Risna A. Onu; Jusna Ahmad; Febriyanti Febriyanti; Novri Youla Kandowangko; Devi Bunga Pagalla; Indriati Husain
Journal of Science, Technology, and Innovation Vol 1 No 2 (2025): December: Inventa: Journal of Science, Technology, and Innovation
Publisher : CV SCRIPTA INTELEKTUAL MANDIRI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.65310/8t6ph238

Abstract

Callus induction is a critical stage in anther culture for supporting the propagation and genetic improvement of local banana cultivars. This study evaluated the effect of different combinations of 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzylaminopurine (BAP) on callus induction from goroho banana (Musa acuminata L.) anthers. Observations were conducted for 30 days after induction, focusing on callus emergence time and morphological characteristics, including color and texture. The results demonstrated distinct responses among treatments. The combination of 1.5 ml 2,4-D and 1.5 ml BAP produced the fastest callus initiation with an average of 13.17 days after induction and generated friable callus with a pale yellow coloration, indicating high cellular activity. Lower growth regulator concentrations delayed callus formation, while higher concentrations promoted color changes associated with early tissue differentiation. No callus formation was observed in the absence of exogenous growth regulators. These findings highlight the importance of balanced auxin and cytokinin concentrations in regulating anther-derived callus induction and provide a physiological basis for further development of goroho banana anther culture systems.
PEMBERDAYAAN MASYARAKAT DESA TUNGGULO MELALUI INOVASI PUPUK CAIR FERMENTASI BERBASIS LIMBAH IKAN DAN KULIT PISANG KEPOK Muh. Alsere Bardian Sahaba; Rieny Sulistijowati S; Wila Rumina Nento; Hamidah Manteu; Indriati Husain
Jurnal Abdi Insani Vol 13 No 4 (2026): Jurnal Abdi Insani
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/abdiinsani.v13i4.3375

Abstract

Indonesian farmers still largely depend on chemical fertilizers, which in the long term can reduce soil quality and increase agricultural production costs. In Tunggulo Village, Tilongkabila District, Bone Bolango Regency, the use of organic waste such as fish waste and banana peels is still very limited and is generally only discarded. However, this waste contains nutrients that can be used as raw materials for making liquid organic fertilizer through a fermentation process. Therefore, community empowerment activities are needed to increase knowledge and skills in processing organic waste into environmentally friendly and economically valuable liquid fertilizer. This community service activity used a pre-test and post-test design to measure changes in participants' knowledge before and after training. The activity was carried out in three main stages, namely preparation, counseling, and training in the production of fermented liquid fertilizer based on fish waste and kepok banana peels. A total of 20 participants who were members of farmer groups were actively involved in the activities. The results of the activities showed an increase in participants' understanding after attending the counseling and training. The average pre-test score of 7.70% shows that participants' initial knowledge of fermented liquid fertilizer was still very low, while the post-test score increased to 81.83% after the activity was carried out. This increase shows that demonstration-based extension and training methods are effective in improving community knowledge and skills. Participants were also able to practice the process of making liquid fertilizer independently and showed interest in developing production on a household scale. This activity not only increased community awareness of organic waste management but also opened up opportunities for the development of sustainable agriculture-based village businesses.