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All Journal Jurnal Hortikultura Indonesia (JHI) Jurnal Agroteknotropika Berkala Ilmiah Pertanian Jurnal Produksi Tanaman Jurnal Penelitian Pertanian Terapan JPT : JURNAL PROTEKSI TANAMAN (JOURNAL OF PLANT PROTECTION) Abditani : Jurnal Pengabdian Masyarakat Madaniya Bioscientist : Jurnal Ilmiah Biologi Jurnal Sains dan Teknologi Pertanian BIOVALENTIA: Biological Research Journal Jurnal Pengabdian Masyarakat Teknologi Pertanian Jurnal lahan Pertanian Tropis Al-Hayat: Natural Sciences, Health & Environment Journal International Journal of Technology and Education Research Inventa: Journal of Science, Technology, and Innovation
Indriati Husain
Universitas Negeri Gorontalo
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Genetic Variation of Sorghum (Sorghum bicolor L.) at Several Colchicine Concentrations Based on Agronomic Characteristics Adam, Marta; Husain, Indriati; Yamin, Mayasari; Nurdin; Dude, Suyono
International Journal of Technology and Education Research Vol. 3 No. 04 (2025): October - December, International Journal of Technology and Education Research
Publisher : International journal of technology and education research

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.63922/ijeter.v3i04.2523

Abstract

Sorghum (Sorghum bicolor L.) serves as an alternative food source to rice due to its rich nutritional content. The development of sorghum through breeding can be facilitated using colchicine. This study aimed to: (1) identify sorghum varieties exhibiting the best performance based on agronomic traits; (2) determine the effective colchicine treatment for increasing genetic variation in sorghum varieties; (3) identify agronomic traits with high genetic variability influenced by multiple genes; and (4) evaluate the best interaction between colchicine and variety based on sorghum agronomic traits. The study was conducted from January to April 2025 in the Greenhouse of the Agricultural Assembly and Modernization Agency, Gorontalo. The experiment employed a factorial Completely Randomized Design (CRD), where the first factor consisted of two varieties, Numbu and Kawali, and the second factor comprised four colchicine concentrations: 0 ppm, 750 ppm, 1000 ppm, and 1250 ppm. Each treatment was replicated three times, resulting in a total of 24 experimental units. The results indicated that the Numbu variety exhibited the highest genetic variability based on leaf area; a colchicine concentration of 750 ppm effectively enhanced sorghum plant variability; plant height, panicle length, and leaf area displayed high genetic variation and were controlled by multiple genes; and the interaction of the Numbu variety with 750 ppm colchicine showed the most favorable performance based on the selection traits of panicle length, plant height, and leaf area. Therefore, this combination holds potential for the colchicine-based development of sorghum
Induction Of In Plantain (Musa acuminata L.) Using The Growth Regulators 2,4-Dichlorophenoxyacetic & BAP (Benzyl Amino Purin) Nur Fitriani, Defy; Indriati Husain; Sutrisno Hadi Purnomo
International Journal of Technology and Education Research Vol. 3 No. 04 (2025): October - December, International Journal of Technology and Education Research
Publisher : International journal of technology and education research

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.63922/ijeter.v3i04.2608

Abstract

Plat propagation through is a method of prodycing new plants by growing unspecified clusters (callus) from small pieces of tissue (explants) such as Gapi banana flowers. This study seeks to determine the appropriate doses of 2,4-D and BAP for callus formation. The study was conducted from September to October 2025 at the Tissue Culture Laboratory of Gorontalo State University, using male Gapi banana flowers explants. The experiment used a randomized block design (RBD) involving two factors: 2,4-D (2ppm) and BAP (5, 10, 15 ppm). The percentage of live explants (%) was observed weekly for 30 days. Final observation included browning, The results showed that the addition of 2,4-D and BAP to the medium greatly affected the percentage of live explants. Without these substances, the explants turned brown. Fungal contamination was dominant at D0 (control) and D2 (2 ppm 2,4-D + 10 ppm BAP). The callus was white/cream to greenish in color, with a firm texture. The percentage of callus-forming explants (%) increased with the addition of BAP (5, 10 and 15ppm), significantly affecting callus formation.
Test of Bamboo Leaf Bacterial Potential on the Growth and Yield of Shallot Plants (Allium ascalonicum L.) Sulle, Atrieda; Husain, Indriati; Irhamnawati Pulogu, Siska; Zakaria, Fauzan; Yamin, Mayasari
International Journal of Technology and Education Research Vol. 4 No. 01 (2026): January- March, International Journal of Technology and Education Research (IJ
Publisher : International journal of technology and education research

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.63922/ijeter.v4i01.2766

Abstract

This research was carried out at the Laboratory of the Department of Agrotechnology, Faculty of Agriculture, Gorontalo State University and at the Green House of the Gorontalo Agricultural Assembly and Modernization Agency from December 2024 to April 2025. This study aims to determine the effect of soaking onion bulbs with bamboo leaf bacterial isolate on the growth and yield of shallot plants and to obtain bamboo leaf bacterial isolate that has the potential to affect the growth and yield of shallot plants. The study was designed using a Random Design Group (RDG) with a suspension treatment of bamboo leaf bacterial isolates consisting of seven types of bacterial isolates that have not yet been identified, namely, X1, X2, X3, X4, X5, X6, X7, and X0 as controls (without bacterial suspension). The parameters measured were plant height, number of leaves, number of seedlings, number of bulbs, weight of wet bulbs, and weight of dry bulbs. The observation data was analyzed using Analysis of Variance (ANOVA α = 5%), and if the results were significantly different, DMRT was further tested at the level of 5%. The results showed that the X2 bacterial isolate suspension treatment had a real effect on the number of seedlings, and had a good influence on the parameters of the number of bulbs and the weight of dry bulbs.
Induksi Kalus dari Antera Pisang Goroho (Musa acuminata L.) dengan Menggunakan 2,4-D dan BAP Risna A. Onu; Jusna Ahmad; Febriyanti, Febriyanti; Novri Youla Kandowangko; Devi Bunga Pagalla; Indriati Husain
Journal of Science, Technology, and Innovation Vol 1 No 2 (2025): December: Inventa: Journal of Science, Technology, and Innovation
Publisher : CV SCRIPTA INTELEKTUAL MANDIRI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.65310/8t6ph238

Abstract

Callus induction is a critical stage in anther culture for supporting the propagation and genetic improvement of local banana cultivars. This study evaluated the effect of different combinations of 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzylaminopurine (BAP) on callus induction from goroho banana (Musa acuminata L.) anthers. Observations were conducted for 30 days after induction, focusing on callus emergence time and morphological characteristics, including color and texture. The results demonstrated distinct responses among treatments. The combination of 1.5 ml 2,4-D and 1.5 ml BAP produced the fastest callus initiation with an average of 13.17 days after induction and generated friable callus with a pale yellow coloration, indicating high cellular activity. Lower growth regulator concentrations delayed callus formation, while higher concentrations promoted color changes associated with early tissue differentiation. No callus formation was observed in the absence of exogenous growth regulators. These findings highlight the importance of balanced auxin and cytokinin concentrations in regulating anther-derived callus induction and provide a physiological basis for further development of goroho banana anther culture systems.
RESPON PERTUMBUHAN DAN PRODUKSI TANAMAN JAGUNG MANIS (Zea mays saccharata Sturt L .) PADA SISTEM OLAH TANAH DAN PESTISIDA NABATI Said, Sutra A; Jamin, Fitriah Suryani; Dama, Hasna; Husain, Indriati; Dude, Suyono
PLANTKLOPEDIA: Jurnal Sains dan Teknologi Pertanian Vol 6 No 1 (2026): Maret
Publisher : Program Studi Agroteknologi, Fakultas Sains dan Teknologi, Universitas Muhammadiyah Siddenreng Rappang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55678/plantklopedia.v6i1.2639

Abstract

Sweet corn (Zea mays saccharata Sturt L.) is a horticultural commodity with high economic value and broad development prospects in Indonesia. However, its production still faces several constraints, including declining productivity due to suboptimal cultivation practices and disturbances from plant pests. This study aimed to evaluate the effects of tillage systems and botanical pesticides, as well as their interaction, on the growth and yield of sweet corn. The research was conducted in Matinan Village, Gadung District, Buol Regency, Central Sulawesi Province. The experiment employed a factorial randomized block design with two factors. The first factor was the tillage system, consisting of two levels: no-tillage (NT) and minimum tillage (MT). The second factor was botanical pesticide application, consisting of three levels: no pesticide (control), 100 mL/L papaya leaf extract (P1), and 100 mL/L soursop leaf extract (P2). The results showed that both tillage systems, no-tillage and minimum tillage, had no significant effect on the growth and yield of sweet corn, including plant height, number of leaves, leaf area, flowering percentage, ear length, and fresh weight of 100 seeds. The application of botanical pesticides made from papaya and soursop leaf extracts also had no significant effect on all observed parameters. Keywords: botanical pesticide; growth; minimum tillage; no-tillage; sweet corn
Co-Authors Adam, Marta Adju, Fitria Y.Hs. Agus Purwito Ali Husni Angry Pratama Solihin Dama, Hasna Devi Bunga Pagalla Devi Bunga Pagalla Dude, Suyono Eyato, Rika Fauzan Zakaria febriyanti febriyanti Fitriah S. Jamin Hasan, Erfina N Hasna Dama Hasna Dama Irhamnawati Pulogu, Siska Isima , Lindawaty Ismail, Fatmawati Jusna Ahmad Kadek Setiawati Kikin H Mutaqin Lamante, Nazwa S. Lindawaty Isima Marleni Limonu Mayasari Yamin, Mayasari Miftahul Fikriyah Adudu Muhammad Arief Azis Ngadi, Suleman Novri Youla Kandowangko Nur Fitriani, Defy Nurdin . Nurmi Rahman Yusuf, Abd Risna A. Onu Rully Tuiyo Said, Sutra A Saleh, Risky Yanto Silvana Apriliani Silviana Arsyad Silviana Arsyad Slamet Susanto Sulle, Atrieda Surdaya, Tedy Sutrisno Hadi Purnomo Sutrisno Hadi Purnomo Suwatan, Silvany Syamsul Bahri Wawan Pembengo Yunnita Rahim Zainudin Antuli