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All Journal HAYATI Journal of Biosciences Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology BIOTROPIA - The Southeast Asian Journal of Tropical Biology The Journal of Experimental Life Sciences (JELS) Jurnal Kelautan : Indonesian Journal of Marine Science and Technology Jurnal EECCIS Journal of Innovation and Applied Technology Research Journal of Life Science Journal of Environmental Engineering and Sustainable Technology Indonesian Green Technology Journal Jurnal Pembangunan dan Alam Lestari REKAYASA JFMR (Journal of Fisheries and Marine Research) Omni-Akuatika Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Riset Akuakultur Samakia: Jurnal Ilmu Perikanan PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Journal of Aquaculture and Fish Health Jurnal Akuakultura Universitas Teuku Umar JATI EMAS (Jurnal Aplikasi Teknik dan Pengabdian Masyarakat) Jurnal Pengabdian pada Masyarakat Ilmu Pengetahuan dan Teknologi Terintegrasi Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Journal of Fisheries & Marine
Maftuch Maftuch
Faculty Of Fisheries And Marine Science, Brawijaya University, Malang, Indonesia
Agriculture, Biological Sciences & Forestry Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Neuroscience Physics Public Health Social Sciences Veterinary Other

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Journal : Research Journal of Life Science

 1 
Phytochemical Analysis and in vitro Antibacterial Activities of Seagrass Enhalus acoroides against Staphylococcus aureus Desy Setyoningrum; Ade Yamindago; Syarifah Hikmah J. S; Maftuch Maftuch
Research Journal of Life Science Vol 7, No 2 (2020)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2020.007.02.2

Abstract

Several studies of marine bioactive compounds have been carried out using seagrass. Enhalus acoroides is a type of seagrass that has bioactive compounds including alkaloids, flavonoids, tannins, steroids, and saponins that have potential as an antibacteria. The aim of study is to investigate the phytochemical compound contained in the crude extract of Enhalus acoroides and its antibacterial activities of Enhalus acoroides against Staphylococcus aureus. The seagrass was collected, washed, dried, grind and exposed to extraction by vacum rotary evaporator at temperature of 40°C and was analyzed for their phytoconstituents. Further, the crude extract was tested against pathogenic bacterial at different concentrations using disc diffusion method to determine the effect of bioactive compounds in E. acoroides to bacteria. In the present study, qualitative test of phytochemical from ethanol extract of Enhalus acoroides leaves contained phytochemical compound of alkaloids, flavonoids, tannins, steroids and saponins, while the extract of roots contained all of compounds except saponins. Based on research can be concluded, that Enhalus acoroides also has antibacterial activity against Staphylococcus aureus that showed by the presence of inhibition zone. The present finding suggests that the extract of seagrass Enhalus acoroides can be used an antibacterial agent from marine
Antibacterial Activity of Dayak Onion Bulbs (Eleutherine palmifolia (L) Merr) Ethanol Fraction against Pseudomonas fluorescens and Its Secondary Metabolite Analysis Immaria Fransira; Annisa Fitri Anggreini; Uun Yanuhar; Maftuch Maftuch
Research Journal of Life Science Vol 6, No 2 (2019)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (487.206 KB) | DOI: 10.21776/ub.rjls.2019.006.02.3

Abstract

Pseudomonas fluorescens is a gram-negative pathogenic bacteria which is one of the problems in fish farming that can causes death in fish. The alternative to overcome this bacterial attack is by using of natural material such as dayak onion bulbs (Eleutherine palmifolia (L) Merr), which are known to have secondary metabolites. The purpose of this research was to determine the antibacterial activity against P. flourescens that contained in the dayak onion bulbs (E. palmifolia (L) Merr) ethanol fraction and the content of antibacterial compounds from the best dayak onion bulbs (E. palmifolia (L) Merr) ethanol fraction. The method used in this research was antibacterial test with MIC (Minimum Inhibitory Concentration) followed by disc test and FTIR (Fourier Transform Infrared Spectroscopy) to analysis secondary metabolite. Fractionation was conducted using Chloroform: Methanol (9:1 v/v). MIC test result showed that 100 ppm was the minimum concentration that can inhibit the growth of P. fluorescens. Disc test reported that fraction 4 was the best fraction, with the dominant compounds that contained in the bulbs was phenolic compound based on FTIR test. So, the fraction of dayak onion bulbs (E. palmifolia (L) Merr) can be used as potential leads to discover new drugs.
Survey of Hawksbill Turtle (Eretmochelys imbricate) Health Condition in Terms of Parasites and Microbes in Alas Purwo National Park, Indonesia Qurrota A'yunin; Happy Nursyam; Sri Andayani; Maftuch Maftuch; Lukluk Intan Maftuchah
Research Journal of Life Science Vol 4, No 1 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (721.704 KB) | DOI: 10.21776/ub.rjls.2017.004.01.5

Abstract

Indonesian waters have six types of turtles that can live, spawn and breed. Sea turtle conservation becomes an important and urgent program to be done in order to protect and save sea turtle population in Indonesia. One of the factors that most affect the turtle population is the cause of degradation of pathogenic factors. Alas Purwo National Park, East Java, there is some communities that have activities turtle conservation. Conservation is done by securing and protecting turtle eggs. Turtle eggs that have hatched are released into the sea once it is ready. This study aims was to determine the type of bacteria and fungi that infect hatchlings and environmental factors that influence. This research is descriptive method to Hawksbill turtle (Eretmochelys imbricate) is by way of random sampling. Sampling of microbes in sea turtle was conducted using cotton swab method and then microbes was cultured and indentified in laboratory. The results showed The kind of parasites and microbes which were indentified in hatching and adult Hawksbill sea turtles were fungus with genus Aspergillus sp., Geotrichum sp., Fusarium sp., and Gliocladium sp. ; bacteria are Pseudomonas aeruginosa and Enterobacter cloaceae; and parasite is Chelonibia testudinaria barnacles.  The parameter average value of water in pond indicated 28.1 – 29.2°C for temperature, 32 - 34 ‰ for salinity, 7.78 – 8.2 for pH, and 3.86 – 4.21 mg/L for DO. 
Survey of Hawksbill Turtle (Eretmochelys imbricate) Health Condition in Terms of Parasites and Microbes in Alas Purwo National Park, Indonesia Qurrota A'yunin; Happy Nursyam; Sri Andayani; Maftuch Maftuch; Lukluk Intan Maftuchah
Research Journal of Life Science Vol 4, No 1 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (721.704 KB) | DOI: 10.21776/ub.rjls.2017.004.01.5

Abstract

Indonesian waters have six types of turtles that can live, spawn and breed. Sea turtle conservation becomes an important and urgent program to be done in order to protect and save sea turtle population in Indonesia. One of the factors that most affect the turtle population is the cause of degradation of pathogenic factors. Alas Purwo National Park, East Java, there is some communities that have activities turtle conservation. Conservation is done by securing and protecting turtle eggs. Turtle eggs that have hatched are released into the sea once it is ready. This study aims was to determine the type of bacteria and fungi that infect hatchlings and environmental factors that influence. This research is descriptive method to Hawksbill turtle (Eretmochelys imbricate) is by way of random sampling. Sampling of microbes in sea turtle was conducted using cotton swab method and then microbes was cultured and indentified in laboratory. The results showed The kind of parasites and microbes which were indentified in hatching and adult Hawksbill sea turtles were fungus with genus Aspergillus sp., Geotrichum sp., Fusarium sp., and Gliocladium sp. ; bacteria are Pseudomonas aeruginosa and Enterobacter cloaceae; and parasite is Chelonibia testudinaria barnacles.  The parameter average value of water in pond indicated 28.1 – 29.2°C for temperature, 32 - 34 ‰ for salinity, 7.78 – 8.2 for pH, and 3.86 – 4.21 mg/L for DO. 
Hematological Analysis of Nile Tilapia (Oreochromis niloticus) and Striped Catfish (Pangasius hypophthalmus) using Hematology Analyzer Tool Software at Fish Breeding Center Jojogan, Tuban, East Java Maftuch Maftuch
Research Journal of Life Science Vol 5, No 2 (2018)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (439.18 KB) | DOI: 10.21776/ub.rjls.2018.005.02.4

Abstract

Nile tilapia and Striped Catfish can be classified as freshwater fish species that have great cultivation potential. Inadequate cultivation management leads to the occurrence of disease attacks on fish. Hematological examination was applied to determine the level of fish health. This research was aimed to know the difference between erythrocyte cell and leucocyte cell count by using Hematology Analyzer Tool Software (computer based) and hand tally counter (human based), and to know the description of hematocrit component, hemoglobin and leucocytes differential on tilapia fish and Striped Catfish. The methods employed in this research were experimental method and descriptive method. The results showed that the calculation of erythrocyte cells and leucocyte cells by using Hematology Analyzer Tool Software was similar to the calculation by using hand tally counter. It was because the result of t value was smaller than t of the 5% table. Based on the description of the hematocrit component, hemoglobin and leucocyte differentials, it could be said that nile tilapia and Striped Catfish were within the range of abnormalities.
The Effect of Hermetia illucens larvae Formulation as Immunostimulant on Non-Specific Immune Response of Cyprinus carpio Challenged by Aeromonas salmonicida Mauludia, Farida; Maftuch, Maftuch; Nurin, Febi Nadhila; Sebastian, Andhang
Research Journal of Life Science Vol 8, No 3 (2021)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2021.008.03.9

Abstract

Cyprinus carpio is one type of freshwater fish. In Indonesia, carp outbreaks in carp since June 2002 have raised concerns, especially for fish farmers. One of the factors causing the outbreak of this disease is the bacteria Aeromonas salmonicida, which can cause fish to get sick and even die. Immunostimulants with natural ingredients that are environmentally friendly and can be used to increase the immune response of fish, one of which is maggot. The data analysis used in this study was a simple completely randomized design (CRD) with 6 treatments difference in the dose of maggot as an immunostimulant (0%, 25%, 50%, 75%, 100%) and three replications. The independent variable is the difference in the dose of maggot as an immunostimulant. The dependent variables include survival rate, total leucocytes, differential leukocytes, and water quality (pH, temperature, and DO). The FT-IR test results obtained that the best compound content in maggot flour is flavonoid compounds where these compounds can increase immune response. So, it is proven by phytochemical tests with positive maggot flour containing flavonoids, tannins, and alkaloids. In total erythrocytes, hematocrit, hemoglobin, total leukocytes, and differential leukocytes showed the best immune response in treatment C with a dose of 75% maggot.
Co-Authors A'yunin, Qurrota Abdul Rahem Faqih Achmad Noercholis Ade Yamindago Agus Budiarto Ahmad Fahrul Syarif Amira Baihani Andhang Sebastian Andriyanto, Septyan Annisa Fitri Anggreini Ari Wahjudi Arief Prajitno Arif Hoetoro Arning Wilujeng Ekawati Asep A. Prihanto Ash Shufi, Abdillah Hanan Astuti, Retno Tri Asus Maizar Suryanto H Bramantiyo Satriyo Wicaksosno D. Ardiansyah Daniar Kusumawati Desy Setyoningrum DIAH AYU SATYARI UTAMI Dwi R. Fatmawati E. Sanoesi Ellana Sanoesi Endang Dewi Masithah Fadjar, Mohammad Farikhah, Reghita Dwi Febby Hadi Setyawan Febi Kesid Haryo Wibisono Febi Nadhila Nurin Friyuanita, Friyuanita Gede Angga Krishna Fariestha H. Suprastyani Happy Nursyam Hariati, Anik Martinah Heny Suprastyani Heny Suprastyani Heny Suprastyani, Heny Herlina, Cucun Hermawan Meidy Kurnianto Ihsan Sanggar Rahman Ika K. Nisyak Immaria Fransira Ismaningdyah Kurniawati Juanda, Eki Karimah Karimah Lila Gardenia M. Aziz Muslim Marsoedi Marsoedi Mauludia, Farida Mochamad Awaludin Adam Moh. Awaludin Adam MOHAMAD FADJAR Mohammad Bisri Mohammad Holil Lulloh Muhammad Arif Nasution Muhammad Arif Nasution Muhammad Awaluddin, Muhammad Muhammad Dailami, Muhammad Muhammad Musa Muhammad Musa Muhammad Nursid Mu’amar Abdan N. Farida N. Habibah Nabilla Anggi Juliyanto Nafiqoh, Nunak Novita, Hessy Nuranti Anarkhis Nurin, Febi Nadhila Pratama, Vian Dedi Prihanto, Asep A. Putra, Gede A. A. K. Putra, Mirsa F. R. Rinaldi Redi Bintarto, Redi Rudianto Raharjo Runi Asmaranto Samuki, Khairul Sanoesi, Ellana Sebastian, Andhang Setyawan, Fahreza Okta Soelistyoadi, Rachmat Noer Sofian, Sofian Sri Andayani Sri Andayani Sri Rahayu Sri Widyarti Sugiarto S Suhermanto, Achmad Sukarni Sukarni Supriatin, Febriyani Eka Syarifah Hikmah J. S Teguh Dwi Widodo Tirani, Elisabeth Vendrita Damayanti Putri Veryl Hasan W., Denny Widya Yahya Yahya Yanuhar, Uun Yenny Risjani Yuni Kilawati Yusuf Arif Wahyudi Zulfadhli Zulfadhli Zulfadhli Zulfadhli