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All Journal HAYATI Journal of Biosciences International Journal of Biosciences and Biotechnology Buletin Udayana Mengabdi Bumi Lestari Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology JURNAL HUBUNGAN INTERNASIONAL Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) Bawal : Widya Riset Perikanan Tangkap Folia Medica Indonesiana SIMBIOSIS Jurnal Biologi Udayana
Ngurah Intan Wiratmini
Program Studi Biologi Fakultas MIPA Universitas Udayana, Bali
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Energy Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Library & Information Science Medicine & Pharmacology Neuroscience Public Health

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Diameter Lumen Trakea dan Bronkus Tikus (Rattus novergicus) yang Diberi Infusa Bunga Telang (Clitoria ternatea L.) dan Paparan Asap Rokok Elektrik Apriliani, Sinta; Wiratmini, Ngurah Intan; Sukmaningsih, A. A. S. A.
Buletin Anatomi dan Fisiologi Volume 10, Nomor 1, Tahun 2025
Publisher : Departemen Biologi Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/baf.10.1.2025.1-9

Abstract

Rokok elektrik dikembangkan untuk mengurangi kecanduan masyarakat terhadap rokok. Rokok elektrik mengandung bahan kimia yang bersifat karsinogen. Tanaman telang (Clitoria ternatea) memiliki aktivitas antioksidan. Penelitian ini bertujuan untuk mengetahui aktivitas antioksidan infusa bunga telang terhadap penyempitan diameter lumen trakea dan bronkus tikus diterpapar rokok elektrik. Penelitian ini menggunakan 30 ekor tikus jantan (Rattus novergicus) dengan umur sekitar 2-3 bulan dan berat badan sekitar 150-200 g. Penelitian ini bersifat eksperimental dengan Rancangan Acak Lengkap (RAL) yang dibedakan menjadi 5 kelompok, yaitu K+ (0,6 mL liquid vape), K- (normal), (0,6 ml liquid vapor + 9 mg/200 g BB infusa bunga telang), P2 (0,6 ml liquid vapor + 18 mg/200 g BB infusa bunga telang) dan P3 (0,6 ml liquid vapor + 36 mg/200 g BB infusa bunga telang). Perlakuan diberikan selama 35 hari. Hasil penelitian dianalisis dengan uji Oneway ANOVA dan dilanjutkan dengan uji Duncan. Hasil yang diperoleh menunjukkan bahwa infusa bunga telang terbukti memiliki aktivitas antioksidan dalam mencegah terjadinya penyempitan diameter lumen trakea dan bronkus, dibuktikan dengan hasil analisis statistik yang menunjukkan adanya perbedaan yang nyata (P<0,05). Infusa bunga telang dengan dosis 36/200 mg memberikan pengaruh yang optimal dalam mencegah penyempitan diameter lumen trakea dan bronkus tikus setelah diberi paparan asap rokok elektrik. E-cigarettes were developed to reduce people's addiction to cigarettes. E-cigarettes contain chemicals that are carcinogens. The telang plant (Clitoria ternatea) has antioxidant activity. This study aims to determine the antioxidant activity of telang flower infusa against the narrowing of the diameter of the lumen of the trachea and bronchi of rats exposed to e-cigarettes. This study used 30 male rats (Rattus novergicus) with an age of about 2-3 months and a body weight of about 150-200 g. This research was experimental with a Completely Randomized Design (CRD) which divided into five treatment groups, namely K+ (0.6 mL liquid vape), K- (normal), (0.6 ml liquid vapor + 9 mg/200 g BB infuse butterfly pea flower), P2 (0.6 ml liquid vapor + 18 mg/200 g BB of butterfly pea flower infusion) and P3 (0.6 ml liquid vapor + 36 mg/200 g BB of butterfly pea flower infusion). The treatment is given for 35 days. The result of the study was analyzed with Oneway ANOVA test and continued with Duncan test. The results showed that the lumen diameter in the K+ group was smaller than in the other groups. Butterfly flower infusion was proven to have antioxidant activity in preventing narrowing of the lumen diameter of the trachea and bronchi, as evidenced by the results of statistical analysis which showed a significant difference (P<0.05). Telang flower infusion given at a dose of 36/200 mg provided an optimal effect in preventing the narrowing of the lumen diameter of the trachea and bronchi of rats after being exposed to e-cigarette smoke.
COVID-19 Disease Severity and Blood Test Results in Unvaccinated and Vaccinated Patients at Bhayangkara Hospital, Denpasar, Indonesia Muyasaroh, Chanif Lutfiyati; Wiratmini, Ngurah Intan; Sukmaningsih, Anak Agung Sagung Alit
Folia Medica Indonesiana Vol. 59, No. 2
Publisher : Folia Medica Indonesiana

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Abstract

Highlights: This original research report on the differences in disease severity and blood test results between unvaccinated and vaccinated COVID-19 patients is quite novel, as the COVID-19 pandemic was still happening when this study was carried out. This original research report offers information on the advantages of the COVID-19 vaccines and provides data that the public may use as scientific evidence to counter hoaxes. Abstract The COVID-19 pandemic caused by SARS-CoV-2 is an unprecedented event in human history. Vaccines are a safe, long-term solution for addressing the COVID-19 pandemic. This study aimed to investigate the differences in disease severity and blood test results between unvaccinated and vaccinated COVID-19 patients. This study used an analytical observational method with purposive sampling. A total of 90 COVID-19 patients at Bhayangkara Hospital, Denpasar, Indonesia, were divided into three groups: unvaccinated group (V0), two-dose vaccinated group (Vp), and three-dose vaccinated group (Vb). Primary data were collected from July to December 2022, while secondary data were collected from January 2021 to June 2022. The data were analyzed using the Kruskal-Wallis test followed by the Mann-Whitney test, as well as one-way ANOVA test followed by Tukey's honestly significant difference (HSD) test with a confidence interval (CI) of 95% and α of 5%. The results revealed significant differences in disease severity (p<0.001). V0 had a higher percentage of severe (36.7%) and critical (6.7%) symptoms than Vp (severe=10.0%; critical, n=0) and Vb (severe and critical, n=0). The follow-up tests revealed significant differences in disease severity between V0 and Vp (p<0.001), V0 and Vb (p<0.001), as well as Vp and Vb (p=0.001). Blood test results revealed significant differences in lymphocytes (p=0.005), monocytes (p<0.001), monocyte-to-lymphocyte ratio (MLR) (p<0.001), and eosinophils (p=0.037). The follow-up tests revealed significant differences in these four indicators between V0 and Vb, in all parameters except for lymphocytes between V0 and Vp, and in lymphocytes only between Vp vs Vb. In conclusion, unvaccinated patients had a higher percentage of severe and critical symptoms than vaccinated patients. The blood test results revealed significant differences in lymphocytes, monocytes, MLR, and eosinophils. Unvaccinated patients had lower lymphocyte counts, higher MLR levels, and higher monocyte counts than vaccinated patients.
Histological Study of the Restorative Effect of Roselle (Hibiscus sabdariffa Linn.) Tea on the Digestive Organs of Monosodium Glutamate-Induced Mice (Mus musculus Linn.) Sistra, Kanigara Anupama; Wiratmini, Ngurah Intan; Sukmaningsih, A. A.S.A.
Folia Medica Indonesiana Vol. 59, No. 4
Publisher : Folia Medica Indonesiana

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Abstract

Highlights: • This original study examined the antioxidant compounds derived from naturally sourced Hibiscus sabdariffa calyx. • The findings demonstrated that roselle tea offers a viable and cost-effective solution for repairing histological damage to the digestive organs induced by monosodium glutamate. Abstract Monosodium glutamate (MSG) is a commonly used synthetic additive for enhancing food flavor. Excessive use of MSG can cause cytotoxic effects, which disrupt the balance of oxidative stress and free radicals in the body, particularly in the human digestive system. Roselle (Hibiscus sabdariffa) is a plant with red petals renowned for its abundance of beneficial compounds, including polyphenols, flavonoids, anthocyanins, and other antioxidants that function as free radical antidotes. This study aimed to investigate the effect of administering roselle tea and determine the optimal dose for restoring the digestive organs of MSG-induced mice (Mus musculus). The research employed a completely randomized design with a random sampling method. A total of 25 mice were divided into five groups: a negative control group (K-) that received 0.3 mL of distilled water, a positive control group (K+) given 4 mg/g bw of MSG, and three treatment groups (P1, P2, and P3) administered with 4 mg/g bw of MSG along with varying doses of roselle tea (2.6 mg/g bw, 3.9 mg/g bw, and 5.2 mg/g bw, respectively). The treatment was orally administered via gavage for 30 days. The stomach, duodenum, and liver underwent histopathological examination using the paraffin method and hematoxylin-eosin staining. The observed parameters in the stomach and duodenum included necrosis, inflammatory cell infiltration, villous erosion, and epithelial desquamation. Meanwhile, the parameters examined in the hepatic organs were necrosis, inflammatory cell infiltration, and cell degeneration. The Kolmogorov-Smirnov normality test and one-way analysis of variance (ANOVA) were employed to assess the normal distribution and homogeneity of the data. If the data exhibited a normal distribution, Duncan's post-hoc test was conducted. The results revealed significant differences among the groups (p<0.05). In conclusion, the administration of roselle tea effectively recovered the histological damage in the stomach, duodenum, and liver of MSG-induced mice.
Estrous cycle and uterine weight of ovariectomized menopause female rat models after treatment with Leucaena leucocephala (Lam.) de Wit leaf extract Wiratmini, Ngurah Intan; Sukmaningsih, Anak Agung Sagung Alit; Setyawati, Iriani
Folia Medica Indonesiana Vol. 60, No. 2
Publisher : Folia Medica Indonesiana

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Abstract

Highlights: 1. This study found that Leucaena leucocephala leaf ethanol extract has the ability to stimulate the growth of vaginal epithelial cells in ovariectomised rats. 2. Leucaena leucocephala leaf extract can be developed as a safe and cost-effective natural alternative for hormone replacement therapy in premenopausal women and livestock. Highlights: 1. This study found that Leucaena leucocephala leaf ethanol extract has the ability to stimulate the growth of vaginal epithelial cells in ovariectomised rats. 2. Leucaena leucocephala leaf extract can be developed as a safe and cost-effective natural alternative for hormone replacement therapy in premenopausal women and livestock. Abstract Hormone replacement therapy has adverse effects that may cause additional health problems, such as hot flashes, cancer, ischemic stroke, and death. Phytoestrogen is an alternative ingredient used for hormone replacement therapy with less risk. The Leucaena leucocephala (Lam.) de Wit plant is widely used as a medicinal ingredient and animal feed. This study aimed to determine the effect of Leucaena leucocephala leaf extract on the uterine weight and estrous cycle of ovariectomized rats. This research used a completely randomized control design, which included two treatment groups (P1 and P2) and two control groups (negative (K-) and positive (K+)) with six replications in each group (n=24). The negative control (K-) was given 2 mL of corn oil, while K+ received 0.1 mg/kg bw of 17β-estradiol. P1 and P2 were administered 250 and 300 mg/kg bw of Leucaena leucocephala leaf extract, respectively. The extract was administered daily by gavage for 30 days. Estrous cycle data were obtained on day 31 by previously collecting vaginal swabs twice a day for 15 days. After the rats were sacrificed under anesthesia using chloroform, they underwent surgery to remove the uterine organs for weight measurement. The data were analyzed for normality and homogeneity using the Shapiro-Wilk and Levene tests. The data distribution were not normal, so the Kruskal Wallis test was used to test the hypothesis. If there was a difference between controls and treatments, the analysis continued with the Mann-Whitney test (p<0.05). The controls and treatments exhibited a significant difference in the length of each phase and overall estrous cycle. The post hoc Duncan test revealed that the highest uterine weight was found in 17β-estradiol treated rat. It was significantly different with both extracts treated rats. Both extract effect on uterine weight were not significantly difference. In conclusion, administering Leucaena leucocephala leaf extract results in an increased uterine weight and a normal estrous cycle in ovariectomized rats.
Eksplorasi metode kultur tiga-dimensi sel punca mesenkim: suatu kajian pustaka Widia Meliawati; Ngurah Intan Wiratmini; Elrade Rofaani
Jurnal Biologi Udayana Vol. 29 No. 1 (2025): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2025.v29.i01.p02

Abstract

Kultur tiga dimensi sel punca mesenkim mempunyai lingkungan physicochemical yang relevan secara in vivo. Metode ini mampu mengatasi keterbatasan pada kultur sel 2D dalam meniru morfologi dan fisiologi sel. Penelitian kajian pustaka ini mengeksplorasi berbagai metode kultur 3D, seperti scaffolds berbasis biomaterial, scaffolds berbasis polimer sintetis, metode tanpa berbasis scaffolds, dan kultur berbasis hidrogel yang dianalisa untuk mengevaluasi keunggulan, kekurangan, dan potensinya. Hasil kajian menunjukkan bahwa berbagai macam kultur tiga dimensi menghasilkan spheroid dengan potensi yang berbeda, namun mampu mendukung interaksi antar sel dan interaksi sel-matriks ekstraseluler, meningkatkan viabilitas, dan derivat metabolik sel punca mesenkim untuk digunakan dalam metode pengobatan terkini. Kultur tiga dimensi juga memberikan lingkungan yang stabil untuk pemeliharaan sel karena distribusi oksigen dan nutrisi yang tersebar secara merata sehingga tidak menyebabkan manfaat dari sel punca mesenkim hilang. Dapat disimpulkan bahwa metode kultur tiga dimensi pada sel punca mesenkim mampu mengatasi keterbatasan pada kultur secara dua dimensi karena memiliki lingkungan fisiologis yang mirip jaringan asli. Teknik kultur tiga dimensi dapat meningkatkan interaksi antar sel dan sel dengan matriks ekstraseluler untuk meningkatkan viabilitas, proliferasi, diferensiasi, dan derivat metabolik pada sel punca mesenkim.
KARAKTERISASI DAN ANALISIS POLA PERTUMBUHAN IKAN KEMBUNG (Rastrelliger sp.) YANG DIDARATKAN DI TEMPAT PELELANGAN IKAN KEDONGANAN, BALI: CHARACTERIZATION AND GROWTH PATTERN ANALYSIS OF IKAN KEMBUNG (Rastrelliger sp.) LANDED AT KEDONGANAN FISH AUCTION SITE, BALI Revael Sihombing; Ni Made Suartini; Ngurah Intan Wiratmini
SIMBIOSIS Simbiosis Volume 13 No. 2 2025
Publisher : Program Studi Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JSIMBIOSIS.2025.v13.i02.p04

Abstract

ABSTRACT One of Bali's largest fish potential areas is Kedonganan, a coastal region where the majority of residents are fishermen. Kedonganan waters represent a highly promising fish landing site due to its direct proximity to the Indian Ocean. Based on preliminary surveys and interviews with local fishermen, the dominant small pelagic fish species frequently landed in large quantities at the Kedonganan Fish Auction (TPI) are mackerel fish (Rastrelliger sp.). This study aims to investigate the morphology, morphometrics, and growth patterns of these species landed at TPI Kedonganan. This research employed survey and interview methods. The variables examined include fish morphology, fish morphometrics consisting of 15 characters, and the growth patterns of mackerel fish (Rastrelliger sp.). The findings reveal that mackerel fish (Rastrelliger sp.) exhibit a fusiform body shape, terminal mouth, forked caudal fin, and cycloid scales. Morphometric measurements showed an average total length of 217.8 cm. The growth pattern of mackerel fish includes negative allometric (b<3).
Co-Authors A.A.S. A. Sukmaningsih AA Istri Mas Padmiswari Anak Agung Gde Raka Dalem Apriliani, Sinta Arnenda, Gussasta Levi Bram Setyadji Dessy Sukma Wirastuti Elfira Dzikri Ashfahani Elrade Rofaani G.A.A Rina Febrina Gussasta Levi Arnenda I Gede Wiranatha I GUSTI AYU MANIK ERMAYANTI I Made Sara Wijana I Made Sara Wijana I Putu Ari Wijana Dipa I Wayan Andi Yoga Kurniawan I Wayan Kasa I.M.A.S. Wijaya Ida Ayu Nopiari Ida Ayu Ratih Purnama Dewi Inna Narayani IRIANI SETYAWATI Iriani Setyawati Jaqueline Sudiman Job Nico Subagio Joko Raharjo, Sentot Joko Wiryatno Luh Putu Wrasiati MADE PHARMAWATI Maria Antonia Margaretha Ferandez Maria Antonia Margaretha Fernandez Mery Wintari Muyasaroh, Chanif Lutfiyati Ni Desak Made Aprilia Dewi Ni Komang Tia Pramesti Ni Luh Eka Setiasih Ni Luh Suriani Ni Made Rai Suarni Ni Made Suartini Ni Putu Adriani Astiti Ni Putu Ayu Dewi Wijayanti Ni Putu Sri Risa Dewi Ni Wayan Adya Puttry Purna Yogini Ni Wayan Sudatri Putu Titah Kawitri Resen Revael Sihombing Salsabillah Rahma SANG KETUT SUDIRGA shanon lestari Sistra, Kanigara Anupama Siti Masnunah Sri Sulastri Sukma Sushanti Sukmaningsih, A. A.S.A. Wahyu Puji Lestari WAHYU WIDOWATI Widia Meliawati