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All Journal HAYATI Journal of Biosciences International Journal of Biosciences and Biotechnology METAMORFOSA Journal of Biological Sciences Buletin Udayana Mengabdi Jurnal Biologi Udayana Bumi Lestari Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology JURNAL HUBUNGAN INTERNASIONAL Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) Bawal : Widya Riset Perikanan Tangkap Folia Medica Indonesiana SIMBIOSIS Jurnal Biologi Udayana
Ngurah Intan Wiratmini
Program Studi Biologi Fakultas MIPA Universitas Udayana, Bali
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Energy Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Library & Information Science Medicine & Pharmacology Neuroscience Public Health

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Hepatoprotektor Rumput Laut Eucheuma cottonii Pada Tikus Putih (Rattus norvegicus L.) Jantan Yang Diinduksi Natrium Nitrit (Nano2) Ida Ayu Ratih Purnama Dewi; Ngurah Intan Wiratmini; Iriani Setyawati
Metamorfosa: Journal of Biological Sciences Vol 7 No 2 (2020)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2020.v07.i02.p12

Abstract

Sodium nitrite (NaNO2) is a food preservative that is often added to processed meat products. Excessive consumption of NaNO2 can be detrimental to health. One effort to minimize the negative effects caused by NaNO2 is by consuming antioxidants. This study aimed to determine the effectiveness of Eucheuma cotonii seaweed extract on improving blood profile, liver and kidney function in male white rats (Rattus norvegicus) induced by NaNO2. This research used a Completely Randomized Design (CRD) consisting of two control groups and three treatment groups. Each group consists of six replications. Negative control (K-) receive aquades and CMC NA 0,5%. Positive control (K +) was only received 22.5 mg/kgBW NaNO2 for 35 days then on the day 36 until the day 45 only given 0,5% CMC Na. The treatment groups P1, P2, and P3 respectively on day 1 to day 15, only received 22.5 mg/kgBW NaNO2 in the morning, and then day 16 to day 35 received 22.5 mg/kgBW NaNO2 in the morning and received 150 mg/kgBW (P1), 300 mg/kgBW (P2), 450 mg/kgBW (P3) Eucheuma cottoni seaweed extract in the evening. Day 30 to day 45, the treatment groups only received Eucheuma cottoni seaweed extracts at each dose. Blood and liver were collected on day 46. Data analysis of serum alanine transaminase (ALT) and aspartate aminotransferase (AST) enzyme levels and liver histology were performed with Anova statistical test and continued with Duncan post hoc test. The results showed Eucheuma cottonii extract dose of 150 mg / kgBB significantly reduced the level of liver damage seen from the levels of ALT and AST and liver histology (percentage inflammatory cell infiltration, congestion, fatty degeneration and necrosis) in white rats induced by sodium nitrite (NaNO2). Keywords: Preservative, AST, ALT, histopathology, liver
HISTOLOGI TESTIS TIKUS (Rattus norvegicus) JANTAN YANG DIBERI TEPUNG DAUN LAMTORO (Leucaena leucocephala Lamk. de Wit) HASIL PERENDAMAN A.A. Istri Mas Padmiswari; Ngurah Intan Wiratmini; I Wayan Kasa
Metamorfosa: Journal of Biological Sciences Vol 4 No 2 (2017)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2017.v04.i02.p07

Abstract

Lamtoro merupakan salah satu tanaman yang memiliki kandungan protein yang cukup tinggi yaitu berkisar antara 25-35%. Namun, pemanfaatan lamtoro menjadi terbatas karena mengandung zat antinutrisi seperti mimosin. Kandungan mimosin dapat diturunkan melalui beberapa metode salah satunya adalah melalui perendaman dalam air. Penelitian ini bertujuan untuk mengetahui pengaruh dari pemberian tepung daun lamoro hasil perendaman (TDLP) terhadap histologi testis tikus jantan. Rancangan percobaan yang digunakan adalah Rancangan Acak Lengkap (RAL) yang terdiri dari empat kelompok dengan masing-masing delapan ulangan. Perlakuan berupa pemberian tepung daun lamtoro hasil perendaman yang dicampur dengan pelet komersial dengan aras 100% pakan komersial (tanpa TDLP) sebagai kontrol (P0), 92,5% pakan komersial + 7,5% TDLP sebagai perlakuan 1 (P1), 85% pakan komersial + 15% TDLP sebagai perlakuan 2 (P2) dan 77,5% pakan komersial + 22,5% TDLP sebagai perlakuan 3 (P3). Perlakuan diberikan pada tikus jantan selama 30 hari. Variabel yang diamati pada penelitian ini adalah jumlah sel spermatogenik. Data hasil penelitian diolah menggunakan program statistik komputer (SPSS 16.0 for Windows) dengan menggunakan uji One Way Anova. Hasil penelitian menunjukkan bahwa penambahan TDLP dalam ransum tidak berpengaruh nyata (P>0,05) terhadap jumlah sel spermatogenik. Hal ini menunjukkan bahwa penambahan TDLP hingga aras 22,5% tidak menurunkan jumlah sel spermatogenik.
PELATIHAN PENGADAAN DAN PENGAWETAN BAHAN PRAKTIKUM BIOLOGI UNTUK GURU SMP BIDANG IPA DI KECAMATAN KUBUTAMBAHAN KABUPATEN BULELENG Intan Wiratmini
Buletin Udayana Mengabdi Vol 19 No 3 (2020): Buletin Udayana Mengabdi
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (291.641 KB)

Abstract

Kendala yang dihadapi dalam mengajar IPA khususnya Biologi adalah tidak tersedianya bahan-bahan praktikum terutama untuk anatomi hewan. Selain karena membutuhkan biaya yang tidak sedikit, pengetahuan untuk menyiapkan bahan praktikum yang murah dan efisien belum dikuasai. Tujuan dari kegiatan pengabdian ini adalah memberikan pelatihan/praktikum langsung kepada guru-guru SMP bidang IPA di Kecamatan Kubutambahan Kabupaten Buleleng tentang cara membedah anatomi hewan coba (mamalia), pembuatan herbarium, insektarium dan pengawetannya sehingga bahan-bahan praktikum tersebut selalu tersedia di laboratorium. Metode pengabdian yang digunakan adalah PRA (Participatory Rural Apraisal), pelaksanaan pelatihan dilakukan dengan ceramah, praktikum dan evaluasi. Kegiatan pelatihan dilaksanakan di SMPN1 Kubutambahan dengan jumlah peserta 28 orang guru bidang IPA dari 7 SMPN di Kecamatan Kubutambahan. Hasil akhir dari kegiatan pengabdian menghasilkan beberapa produk, antara lain herbrium, insektarium dan awetan basah hewan mamalia (mencit jantan dan betina)
PENGARUH PEMBERIAN RHODAMIN B TERHADAP SIKLUS ESTRUS MENCIT (Mus musculus L.) BETINA G.A.A Rina Febrina; Ngurah Intan Wiratmini; Ni Wayan Sudatri
Jurnal Biologi Udayana Vol 17 No 1 (2013): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

The research was conducted to identify the effect of rhodamine B on estrous cycle on female mice (Mus musculus L.). Twenty-four female mice were used, and randomly divided into four groups.  Rhodamine B was given orally (gavage) with a dose of  0 ppm (control), 150 ppm, 300 ppm, and 600 ppm in two estrous cycles.  Treatments were started when mice in estrus phases. The time between first and the next estrus phase were observed from vaginal smear preparation. Data obtained were analysed with One Way Anova.  If the differences were appeared between treatments, then the LSD test was performed. The results showed that rhodamine B was significantly increased the time of estrous cycle (P<0.05).
MOTILITAS DAN VIABILITAS SPERMATOZOA MENCIT (MUS MUSCULUS L.) SETELAH PEMBERIAN EKSTRAK TEMU PUTIH (CURCUMA ZEDOARIA (BERG.) ROSCOE.) Elfira Dzikri Ashfahani; Ngurah Intan Wiratmini; A.A.S.A Sukm aningsih
Jurnal Biologi Udayana Vol 14 No 1 (2010): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

This research was aimed to study the effect of temu putih (Curcuma zedoaria (Berg.) Roscoe.) extract treatment on viability and motility of mice (Mus musculus L.) spermatozoa. The extract was given orally once a day in 35 days. The animals were divided into four groups; one control group and three treatment groups with six replicates. (M0 = control group; M1 = group was given 100 mg/Kg body weight/day; M2 = group was given 200 mg/Kg body weight/day; M3 = group was given 300 mg/Kg body weight/day). The result of the study showed that motility and viability of spermatozoa were decreased significantly (p< 0.05) after receiving temu putih (C. zedoaria (Berg.) Roscoe.) extract for 35 days.
PERTUMBUHAN, HISTOPATOLOGI OVARIUM DAN FEKUNDITAS IKAN NILA MERAH (OREOCHROMIS NILOTICUS) SETELAH PAPARAN PESTISIDA ORGANOFOSFAT IRIANI SETYAWATI; NGURAH INTAN WIRATMINI; JOKO WIRYATNO
Jurnal Biologi Udayana Vol 15 No 2 (2011): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

Fish cultures in rice fields or areas that use water from a pesticide-polluted irrigation or river, are particularly vulnerable to the negative impact of pesticides. This study aims to assess the effect of organophosphates on growth, ovarian histopathology and fecundity of red tilapia (Oreochromis niloticus). After acclimatization, 160 fishes (fingerlink stage) were kept in four ponds sized 2x1,5x0,8 m each. This study used a complete randomized block design and organophosphate administration through the feed. The study used four groups: control, and variations in dose of 2 ml (D1), 6 ml (D2), and 10 ml (D3) of pesticide per 100 grams of pellet, which is given in the morning (treatment) and in the evening (regular feed). Data were taken at week 3, 6, and 9, then the organophosphate treatments were stopped, and the last data were taken at week-18. Statistical analysis was performed by Anova and Duncan's Multiple Range Test. The results showed that the organophosphate pesticide inhibits the growth by reducing fish length and weight, reducing ovarian weight, increasing the number of oocyte atresia, damaging structure of the ovarian histology, and decreasing fecundity by reducing the number of egg production in the ovarian of red tilapia.
GANGGUAN SPERMATOGENESIS SETELAH PEMBERIAN MONOSODIUM GLUTAMAT PADA MENCIT (MUS MUSCULUS L.) A.A.SG.A SUKMANINGSIH; I GUSTI AYU MANIK ERMAYANTI; NGURAH INTAN WIRATMINI; NI WAYAN SUDATRI
Jurnal Biologi Udayana Vol 15 No 2 (2011): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

This study was conducted to investigate the effect of monosodium glutamate (MSG) on spermatogenesis of male experimental mice.using 32 twelve-week old mice. They were divided randomly into 4 groups, each group consisted of 8 mice. One group was used as control, three groups were used as treatment, which received 1.5 mg/g body weight/day, 3 mg/g body weight/day and 4.5 mg/g body weight/day of MSG solution in destilled water for 35 days. The result showed that MSG treatment led to disturbance on spermatogenesis by reduction in some of the parameter studied. There was a significant (p < 0.05) decrease in the number of pachytene spermatocyte and spermatid 15, where as the decrease in spermatogonia was insignificant (p>0.05).
PANJANG SIKLUS ESTRUS MENCIT (Mus musculus L.) YANG DIBERI PEMANIS BUATAN ASPARTAM SECARA ORAL Sri Sulastri; Ngurah Intan Wiratmini; Ni Luh Suriani
Jurnal Biologi Udayana Vol 18 No 2 (2014): JURNAL BIOLOGI
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh pemberian aspartam terhadap panjang siklus estrus mencit betina dewasa. Rancangan yang digunakan dalam penelitian ini adalah Rancangan Acak Lengkap (RAL) dengan 4 perlakuan dan 6 ulangan. Perlakuan P0 sebagai kontrol diberi aquades dan perlakuan P1, P2 dan P3 diberi aspartam dosis10 mg/kg bb; 15 mg/kg bb dan20 mg/kg bb. Aspartam diberikan setiap hari secara oral (gavage) selama 14 hari sebanyak 0,3 ml. Setelah 14 hari, apusan vagina dibuat setiap 8 jam dalam sehari selama dua kali siklus estrus. Variabel yang diamati adalah panjang waktu tiap fase dalam siklus estrus. Hasil analisis menggunakan Uji One Way Anova dan Uji Kruskal Wallis menunjukkan bahwa aspartam secara nyata (P<0,05) memperpanjang siklus estrus dengan peningkatan dosis yang diberikan.
PENGARUH PEMBERIAN EKSTRAK DAUN EKOR NAGA (Rhaphidophora pinnata, Schott) TERHADAP PERKEMBANGAN UTERUS MENCIT (Mus musculus) BETINA YANG TELAH DIOVARIEKTOMI Maria Antonia Margaretha Ferandez; Ngurah Intan Wiratmini; Ni Gusti Ayu Manik Ermayanti
Jurnal Biologi Udayana Vol 19 No 2 (2015): JURNAL BIOLOGI
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

Tanaman ekor naga (Rhaphidophora pinnata, Schott.) mengandung zat aktif dari golongan senyawa alkaloid, flavonoid, saponin, tanin dan triterpenoid/steroid. Zat aktif yang terkandung dalam tanaman ekor naga bersifat fitoestrogen. Tujuan penelitian ini adalah untuk mengetahui pengaruh pemberian ekstrak daun ekor naga terhadap perkembangan uterus mencit (Mus musculus) betina yang telah diovariektomi. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) yang terdiri dari empat kelompok perlakuan dengan masing-masing enam ulangan. PerlakuanP0 sebagai kontrol diberi NaCl 0,9% dan perlakuan P1, P2 dan P3 diberi ekstrak daun ekor naga dengan dosis 50, 100  dan 150 mg/KgBB. Ekstrak daun ekor naga diberikan setiap hari secara oral selama 14 hari sebanyak 0,2 ml dengan metode gavage. Variabel yang diamati adalah bobot uterus, diameter uterus dan ketebalan endometrium. Data yang diperoleh dianalisis dengan One Way Anova dilanjutkan dengan uji Duncan Multiple Range Test (DMRT). Hasil penelitian menunjukkan bahwa pemberian ekstrak daun ekor naga (R. pinnata, Schott) menyebabkan peningkatan ketebalan endometrium sebesar 38,7% dan peningkatan diameter uterus sebesar 30,3% dibanding dengan kelompok kontrol. Dosis optimal ekstrak daun ekor naga (R. pinnata, Schott) yang berpengaruh terhadap perkembangan uterus mencit adalah 100 mg/KgBB.
MAKROZOOBENTHOS PANTAI PERERENAN (KABUPATEN BADUNG): JENIS, STATUS, DAN MANFAATNYA BAGI MASYARAKAT Ngurah Intan Wiratmini; Joko Wiryatno; A.A.G. Raka Dalem
Bumi Lestari Journal of Environment Vol 8 No 2 (2008)
Publisher : Environmental Research Center (PPLH) of Udayana University

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Abstract

The research was undertaken on Pererenan beach in the Regency of Badung, Bali Province, between September and December, 2006. The study sites occur close to the downstream of Bausan creek, the location of which were part of ”Subak Kedungu” irrigation system (east of the creek) and ”Subak Munggu Tegal Lantang” irrigation system (west of the creek). Samples were taken on low tides periods of the intertidal zones of the beach. Samples were stratified randomly taken on three kinds of substrates: on sand and coral substrates on the west, sand substrates in the middle and hard sandstone on the east, each of which consist of five quadrates of 40 cm x 40 cm. Results of this research showed that on the site have been successfully identified 17 species of macrozoobenthic fauna. None of them were classified as protected species. The macrozoobenthic fauna on Parerenan beach had a medium level of index of diversity (IK = 2,598). This meant that, the beach ecosystem in Pererenan was in a stable condition with a medium level of diversity. From the benefit and problems related to their occurrence, it can be described that macrozoobenthic organisms on this study sites, some can be consumed. Besides, there were also some species which dangerous to human because they can cause bleeding, or dangerous hurt caused by sharp needles.
Co-Authors A.A.S. A. Sukmaningsih AA Istri Mas Padmiswari Anak Agung Gde Raka Dalem Apriliani, Sinta Arnenda, Gussasta Levi Bram Setyadji Dessy Sukma Wirastuti Elfira Dzikri Ashfahani Elrade Rofaani G.A.A Rina Febrina Gussasta Levi Arnenda I Gede Wiranatha I GUSTI AYU MANIK ERMAYANTI I Made Sara Wijana I Made Sara Wijana I Putu Ari Wijana Dipa I Wayan Andi Yoga Kurniawan I Wayan Kasa I.M.A.S. Wijaya Ida Ayu Nopiari Ida Ayu Ratih Purnama Dewi Inna Narayani Iriani Setyawati IRIANI SETYAWATI Jaqueline Sudiman Job Nico Subagio Joko Raharjo, Sentot Joko Wiryatno Luh Putu Wrasiati MADE PHARMAWATI Maria Antonia Margaretha Ferandez Maria Antonia Margaretha Fernandez Mery Wintari Muyasaroh, Chanif Lutfiyati Ni Desak Made Aprilia Dewi Ni Komang Tia Pramesti Ni Luh Eka Setiasih Ni Luh Suriani Ni Made Rai Suarni Ni Made Suartini Ni Putu Adriani Astiti Ni Putu Ayu Dewi Wijayanti Ni Putu Sri Risa Dewi Ni Wayan Adya Puttry Purna Yogini Ni Wayan Sudatri Putu Titah Kawitri Resen Revael Sihombing Salsabillah Rahma SANG KETUT SUDIRGA shanon lestari Sistra, Kanigara Anupama Siti Masnunah Sri Sulastri Sukma Sushanti Sukmaningsih, A. A.S.A. Wahyu Puji Lestari WAHYU WIDOWATI Widia Meliawati