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All Journal Jurnal Penelitian Saintek Agrotrop : Journal on Agriculture Science Indonesian Journal of Biotechnology Jurnal Pendidikan Biologi Indonesia Bioeksperimen: Jurnal Penelitian Biologi Jurnal Penelitian Pendidikan IPA (JPPIPA) Caraka Tani: Journal of Sustainable Agriculture Jurnal Bioedukatika Biosfer: Jurnal Pendidikan Biologi Jurnal Pengabdian Masyarakat MIPA dan Pendidikan MIPA Jurnal Pertanian Agros AL-HAYAT: Journal of Biology and Applied Biology Jurnal Pendidikan Sains Indonesia (Indonesian Journal of Science Education) Agroista: Jurnal Agroteknologi Kingdom (The Journal of Biological Studies) IJOBI
Ixora Sartika Mercuriani
Universitas Negeri Yogyakarta
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Neuroscience Physics Public Health

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Journal : Indonesian Journal of Biotechnology

 1 
Selection of Phalaenopsis amabilis L. Blume Orchid Resistance to Hygromycin Ixora Sartika Mercuriani; Aziz Purwantoro; Sukarti Moeljopawiro; Seonghoe Jang; Endang Semiarti
Indonesian Journal of Biotechnology Vol 17, No 2 (2012)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (258.811 KB) | DOI: 10.22146/ijbiotech.16000

Abstract

Examination of Phalaenopsis amabilis orchid resistance to hygromycin antibiotic is an important step to doprior to Agrobacterium-mediated genetic transformation in this orchids using Hygromycin phosphotransferase(HPT) gene as a selection marker in the T-DNA that harboring a desired gene to be transfered. We exposedthe plant on hygromycin containing medium. The experiment was conducted using 6 weeks old P. amabilisprotocorms. These protocorms were subcultured onto NP medium supplemented with various concentrationof Hygromycin (0, 5, 10, 20, 1nd 40 mg/l). The number of survival protocorms were examined every week for4 weeks after subcultured (WAS). The resistancy of hygromycin was calculated as ratio of death protocormsper total protocorms). The result showed that 10 mg/l hygromycin with 1 weeks of application caused deathclose to LD 50. This data indicate that P. amabilis resistance to hygromycin treatment on the appropriateconcentration 10 mg/l, and this concentration can be used for other purposes in orchid system.
Identification and genetic study of lactic acid bacteria from intestine of domestic chickens (Gallus gallus domesticus) Ixora Sartika Mercuriani; Heru Nurcahyo; Bernadetta Octavia; Astuti Astuti; Adinda Yuslia Rukmanandita; Fita Nilasari
Indonesian Journal of Biotechnology Vol 30, No 2 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.85963

Abstract

The gastrointestinal tract is one of the habitats of lactic acid bacteria (LAB). This research aims to identify the LAB isolated from the intestines of domestic chickens (Gallus gallus domesticus) based on phenotypic and genotypic characteristics. The LAB was cultivated on enrichment media MRSA + CaCO3 1% and a selection of isolates based on the halo zone. The phenotypic character identification using morphological, physiological and biochemical tests was analyzed by similarity simple matching (SSM) and a dendrogram (MVSP 3.1). Genotypic identification was made by 16S rRNA gene sequences with PCR methods, sequencing and analysis using BLAST at www.ncbi.nlm.nih.gov. The results show that 87 isolates were isolated based on the halo zone. Phenotypic tests were performed on eight isolates (J6, J15, J18, J28, B11, B24, B25 and B26). The results show that four isolates have similarities with the genus Bacillus, two isolates with the genus Lactobacillus, and two with the genus Streptococcus. The identification based on the 16S rRNA gene of four selected isolates (J15, J28, B24 and B26) showed that J15 was identified as Bacillus cereus (98.56% similarity); J28 as Lactobacillus johnsonii (99.67%); B24 as Bacillus cereus (98.30%), and B26 as Streptococcus pluranimalium (96.68%).
Co-Authors Adinda Yuslia Rukmanandita Afrizal Haris Agus Slamet Akram Akram Anisa Maulidiya* Annisa Ul Furqhoni Aries Bagus Sasongko Astuti Astuti Astuti Ayu Purnamasari Aziz Purwantoro Bekti Sulistya Utami Bernadetta Octavia Candra, Puspa Mega Desi Indriani Dwi Kurniasari Eli Rohaeti Endang Semiarti ENDANG SEMIARTI Endik Deni Nugroho Endik Deni Nugroho Evy Yulianti Fita Nilasari Heru Nurcahyo Heru Nurcahyo HESTIN YUSWANTI Huang, Tzou-Chi Isana Supiah Yosephine Louise Khoirunnisa Khoirunnisa Kun Sri Budiasih Lili Sugiarto Lili Sugiyarto Madhani, Anisa Tiara Miftakhul Khasanah Nur Fitriyana Nurfina Aznam Paramita Cahyaningrum Kuswandi Puspa Mega Candra Putra, Rinaldi Rizal Ratna Dewi Ratnawati Ratnawati Ratnawati Ratnawati Ratnawati Retno Arianingrum Rifqi, Daffa Muhammad Rinaldi Rizal Putra, Rinaldi Rizal Rindang Dwiyani Ririn Budiastuti Seonghoe Jang Septasari, Mistri Setiawan, Andri Dias Sidabutar, Nurhalis Sofiatul Milah Sukarti Moeljopawiro Sukarti Moeljopawiro Suyitno Aloysius Vlorensius Vlorensius Wiharyanti, Risma Wiharyanti, Risma Wika Rinawati Yuyun Fitriani