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All Journal INDONESIAN JOURNAL OF LEGAL AND FORENSIC SCIENCES International Journal of Biosciences and Biotechnology METAMORFOSA Journal of Biological Sciences Buletin Udayana Mengabdi Jurnal Biologi Udayana Jurnal Pendidikan Biologi Undiksha SIMBIOSIS BERITA BIOLOGI Jurnal Biodjati Eksakta : Berkala Ilmiah Bidang MIPA Agro Bali: Agricultural Journal International Journal of Applied Science and Sustainable Development (IJASSD)

ENIEK KRISWIYANTI

Program Studi Magister Ilmu Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Udayana, Denpasar-Bali

Author-ID : 2925713

Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Dentistry Earth & Planetary Sciences Education Energy Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology

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ANALISA KERAGAMAN GENETIK KELAPA RANGDA (Cocos nucifera L.) DI BALI BERDASARKAN PENANDA DNA MIKROSATELIT Ni Luh Putu Rika Sugiantari; I Ketut Junitha; Eniek Kriswiyanti
SIMBIOSIS Vol 3 No 2 (2015)
Publisher : Jurusan Biologi Universitas Udayana

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The research aimed to analyze genetic variation of Rangda coconut in Bali based on microsatellite DNA markers. The young leaf (busung) were collected from Gunaksa village at Klungkung regency, Tulikup village at Gianyar regency, Kubu village at Bangli regency, Sanghyang village, Jembrana regency one sample each, and three samples are from Ngis village at Karangasem regency. Doyle and Doyle DNA extraction method was used to extract DNA from the samples. Three primer pairs of CnCirC3, CNZ05 and CNZ51 were used to amplify DNA samples. Amplicons were electrophoresed on PAGE 10% and stained with silver nitrate. The allele size was determined by plotting the amplicon migration distance on semilog paper. The results showed there were 11 alleles with an average of 13.33 alleles per primer. There were 3 alleles on primer CNZ05, 4 alleles on primer CnCirC3 and CNZ51 each. Heterozigosity expectation (He) value on primer CnCirC3, CNZ05, and CNZ51 were 0,49±0,04, 0,27±0,03 and 0,71±0,06 consecutively. The result showed that Genetic relationship among 7 coconut samples were divided into two big groups. Key word : Rangda coconut, Microsatellite marker, Number allele, Genetic relationship

PROFIL STRUKTUR SERAT IBU TANGKAI DAUN ANTARA INDUK DAN ANAKAN KELAPA (Cocos nucifera L “Rangda”) Ni Made Puspawati; Eniek Kriswiyanti; I Ketut Junitha
SIMBIOSIS Vol I, No 2, Tahun 2013
Publisher : Jurusan Biologi Universitas Udayana

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The research aims to determine the anatomical structure of the leaf stalk fiber Coconut(Cocos nucifera L. "Rangda") on the seedling and parent plant. Sampling was conducted atSanghyang village Melaya district, Regency of Jembrana. Preparations and microscopicobservations made in the laboratory of Plant Development Structure Biology Department,FMIPA Unud. The research was conducted in October 2012 - April 2013. Maceration methodused for preparations sklerenkim fibers and xylem elements. The results showed that theanatomical parent plant and seedling petiole were torque and mostly composed bysclerenchym fibers but in the parent plant more torque than seedling. In the parent plant about81% the sclerenchym cell was torque but in seedling only 13%, with an average number of17 ± 1 torsion of the parent plant and 6 ± 1 in seedling.Keywords: the fiber structure, torque

MANFAAT TANAMAN TERATAI (Nymphaea sp., Nymphaeaceae) di DESA ADAT SUMAMPAN, KECAMATAN SUKAWATI, KABUPATEN GIANYAR, BALI Gusti Ayu Nyoman Budiwati; Eniek Kriswiyanti
SIMBIOSIS Vol 2 No 1 (2014)
Publisher : Jurusan Biologi Universitas Udayana

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his purpose of this research was to determine the benefits of the lotus plant inSumampan Village, District of Sukawati, Gianyar, Bali. The research was conductedfrom 4 to 16 February 2013. The method was used in this study is exploratory survey bydirect observation and interviews with one main informant and 15 KK from 3 banjar. Theresults showed there were 11 kinds of lotus : lotus sudamala ( Nymphoides indica ) (4.54%), yellow lotus (21.21 %), dark blue lotus ( Nymphaea stellata Wild ) (12.12 %), pinklotus (16.66 % ), violet lotus (9.09 %), purple lotus (9.09 %), white lotus (Nymphaeanouchali Burm f.) (18.18 %), light blue lotus (Nymphaea stellata Wild ) (3.03 %), lotustutur (1.51 %), lotus dedari (1.51 %) and lotus brumbun (3.03 %). The lotus plant is usedas a upakara / banten 77.41 % , 16.12 % as ornamental plants, while 6.45 % as amedicine for breast cancer drug, arthritis, headaches, stress, fear, and cleanser the liverand pancreas. As a medicine, lotus plant was used in it’s from as tempel, solutions, pupukand boreh.Key word: exploratory survey, benefits lotus, tempel, pupuk, boreh

FLORAL DIVERSITY IN MINCIDAN VILLAGE, KLUNGKUNG, BALI TO SUPPORT ECOTOURISM Made Ria Defiani; Eniek Kriswiyanti
SIMBIOSIS Vol 7 No 1 (2019)
Publisher : Jurusan Biologi Universitas Udayana

Flora diversity is one of natural resources for living of organism. The study aimed to explore type and usage of plants for medicinal at planned tracking area Mincidan Village to give benefit for education and ecotourism purposes. Method was descriptive analysis by surveying and giving some question to people around the village for plant conservation. Plants that were grown along the tracking site can be grouped as medicinal, ornamental, traditional uses on offerings. Based on the results, the plant can be identified 102 species, 71 genera in 38 families. The plants can be used for material on offering and traditional medicine.

HUBUNGAN KEKERABATAN 12 KULTIVAR BROKOLI (Brassica oleracea L.) BERDASARKAN KARAKTER ANATOMI STOMATA Ni Putu Sri Risa Dewi; Eniek Kriswiyanti; I Gusti Ayu Sugi Wahyuni
SIMBIOSIS Vol 3 No 1 (2015)
Publisher : Jurusan Biologi Universitas Udayana

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This research aims to know the relationship of kinship in 12 cultivars Broccoli grownin Horticultural Nursery at Kembang Merta village Bedugul Bali. Broccoli leaf samples weretaken from Horticultural Nursery at Kembang Merta village Bedugul Bali. The method usedin this research is by utilizing fresh preparations of upside/downside leaf epidermal incisionor Leaf Clearing method, the incision of the preparations and also the observations werecarried out at the Laboratory of Plant Structure and Development Department of Biology,Faculty of Science and Mathematics, Udayana University. The results showed the existenceof variations in epidermal cells, stomata structure, density and density of stomata on the 12cultivars of Broccoli (Brassica oleracea L.) with the range index of similarity between68,79% - 92,18% and formed the five groups that have different similarity indices in eachgroup.Keywords: anatomy, broccoli (Brassica oleracea L.), similarity index of stomata

ANALISIS KEKERABATAN BEBERAPA TANAMAN MANGGA (Mangifera spp.) BERDASARKAN KARAKTERISTIK MORFOLOGI DAN ANATOMI DAUN Luh Putu Melandani; Eniek Kriswiyanti; Made Ria Defiani
SIMBIOSIS Vol 5 No 1 (2017)
Publisher : Jurusan Biologi Universitas Udayana

ABSTRACT The study was aimed to explore the characteristics of morphological, anatomical, and phylogenetic relationship between some types of mango (Mangifera sp.). Research conducted in Structures Laboratory of Plant Development Department of Biological Science, University of Udayana from April 2015 - July 2015. Samples were taken from three districts in Bali, that Denpasar, Gianyar and Badung. Ten species of plants of mango (Mangifera indica : ‘poh arum manis’, ‘poh bukit’, ‘poh gedang’, ‘poh madu’, ‘poh apel’,‘poh golek’, ‘poh sambuk’, ‘poh bali’, ‘poh manalagi’) and Wani (Mangifera caesia.) shows the character of morphology and anatomy of leaves varies as well as the analysis of kinship showed ten types of mangoes grouped into two major groups based on the degree of similarity. Fenogram shows the level of similarity of the ten types of mangoes in Bali grouped (cluster) into two: group I ‘poh arum manis’, ‘poh bukit’, ‘poh gedang’, ‘poh bali’, ‘poh apel’, and group II, namely ‘poh madu’, ‘wani’, ‘poh golek’, ‘poh sambuk’, ‘pohmanalagi’ where two clusters have a degree of similarity ? 63.91%.

JENIS - JENIS TUMBUHAN YANG DIGUNAKAN SEBAGAI BAHAN PERAWATAN KECANTIKAN DI PURI DAMAI DESA SINGAKERTA, KECAMATAN UBUD, KABUPATEN GIANYAR Anak Agung Istri Ratih Gayatri; Eniek Kriswiyanti; I Gusti Ayu Sugi Wahyuni
SIMBIOSIS Vol 3 No 1 (2015)
Publisher : Jurusan Biologi Universitas Udayana

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Indonesia has many variaties of plant which give a benefit for house, clath, meals,medicine even for beauty treatment. A beauty treatment made from chemical agent causing anegative effect, so searching for an alternative agent for beauty treatment without giving anegative side effect was needed. The goal of has research is to know find species of plants,methods of process also how to process the plants become a herbal beauty treatment agent atPuri Damai which located in Singakerta village, district of ubud, Gianyar region. They takedirect observation, description and identification methods. According to the result of theresearch were found 25 species, 19 suku of plants 3 euphorbiaceae, 2 asteraceae, 2araliaceae, 2 zingiberaceae, pandanaceae, asphodelaceae, poaceae, fabaceae, anonaceae,magnoliaceae, arecaceae, laminaceae, malvaceae, cactaceae, oleaceae, apocynaceae,agavaceae, melastomataceae, rutaceae. Hair vertilizer oil mostly was made from the leaf ofthe plant takes around 56% and for massage oil take the flowers around 53 %. The methodswas taken to produce hair vertilizer oil and ingredients was fried in 1 liter frying oil between6 – 8 hours long in little fire. Direction of use for hair vertilizer oil is rubbing the oil on thecalp and hair before is after washing the hair. The use of massage oil the oli to the body andgive a massage.Keywords : Etnobotany, Beauty treatment, Observation.

KARAKTERISASI DAN DISTRIBUSI KELAPA MADAN DI KECAMATAN MANGGIS KABUPATEN KARANGASEM, BALI I Wayan Sumerta Yasa; Eniek Kriswiyanti
SIMBIOSIS Vol 4, No 1 (2016)
Publisher : Jurusan Biologi Universitas Udayana

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The purpose of research to determine themorphological characteristics and distribution of coconut variety mine inManggis district. The research was conducted from February-March 2014 in the villageNgis, Selumbung and ApitYeh. Themethod used in this research is purposive sampling by observation stems ,leaves and fruit based manua lIBPGR. The resultshowed 18 individual coconut Madan are classified intwovarieties: a) Tall coconut (Cocos nucifera L. var. typica) 15individual: ‘Gadang’, ‘Bingin’, ‘Bojog’, ‘Bejulit’, ‘Sudamala’, ‘Bluluk’, ‘Rangda’, ‘Naga’, ‘Bulan’, ‘Udang’, ‘Padma’,‘Pudak Selumbung’, ‘Pudak Ngis’, ‘Mulung’, ‘Bunga’ and b) Dwarf coconut (Cocos nucifera L. var. nana) 3 individual:‘Gading’, ‘Bulan’ dan ‘Gadang’. Distribution: in the Ngis village 15 individuals, in the Selumbung village 7 individuals andin the Apit Yeh village 5 individuals.Keywords: characterization, coconut madan, Cocos nucifera L , distribution

PERKEMBANGAN MIKROGAMETOFIT DAN UJI VIABILITAS SERBUK SARI KELAPA (Cocos nucifera L. “Ancak”) Ni Luh Gede Cerli Tonika Sari; Eniek Kriswiyanti; Ni Nyoman Darsini
SIMBIOSIS Vol I, No 2, Tahun 2013
Publisher : Jurusan Biologi Universitas Udayana

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The research observed the type of pollen shape, development of microgametophyte, andpollen viability of Cocos nucifera L. “Ancak”, that is the pollen structure Cocos nucifera L. usedpreparation with acetolysis method. Development of microgametophyte, and pollen viabilitywith germinated pollen in vitro used 0,8% of agar media in sugar solution 0%, 50%, and 70%.The result showed that the type of pollen shape Cocos nucifera L. “Ancak”: circular, sulkus,media, Subferoidal, P/E: (1.12-1.18). Development of microgametophyte Cocos nucifera L.“Ancak” the flowers are ripe generally at the level of uninucleate (> 60%) and binukleat (<40%).Male gametes (trinukleat) started on day 2,4. percentage of pollen viability in vitro low average(< 2.5%).Keywords: Pollen structure, microgametophyte, viability test, in vitro

UJI VIABILITAS SERBUK SARI SECARA In-Vitro KELAPA (Cocos nucifera L.“Rangda”) DENGAN WAKTU DAN SUHU PENYIMPANAN YANG BERBEDA Sista Nirmala; Eniek Kriswiyanti; AA. Ketut Darmadi
SIMBIOSIS Vol I, No 2, Tahun 2013
Publisher : Jurusan Biologi Universitas Udayana

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This aims of the research was to determine the structure and pollen viability of RangdaCoconut (Cocos nucifera L. “Rangda”) stored at different time and temperatures. Pollen sampleswere collected from Gianyar, Klungkung and Negara. The experiment was conducted at theLaboratorium of Forensic and the Laboratorium of Plant Development and Structure, UdayanaUniversity, from October 2012 to January 2013. Pollen structure was analyzed with acetolysemethod, while pollen viability was analyzed in-vitro. The results showed that the structure of thepollen of “Rangda” coconut was circular, media, monosulcate, Subferoidal-Prolat, Sferoidal.Pollen viability of “Rangda" coconut was low (less than 3%). Pollen stored in the freezer has thebest viability, which continually increased until the 2nd weeks, then declined after that, whiepollen viability stored at room temperature tend to decline up to the fourth weeks.Keywords: structure, viability, pollen, time, storage

Co-Authors A.A.S. A. Sukmaningsih Alprida Yanti Anak Agung Istri Ratih Gayatri Anak Agung Ketut Darmadi Desak Nyoman Budiningsih Dewa Ayu Sri Ratnani Eka Budi Mursafitri Gusti Ayu Nyoman Budiwati Gusti Ayu Nyoman Budiwati Hanas, Dicky Frengky I Gusti Ayu Sugi Wahyuni I Ketut Junitha I KETUT MUKSIN I Komang Alit Adi Sanjaya I Made Saka Wijaya I NYOMAN RAI I Putu Sudiartawan I Wayan Sumerta Yasa I.M.S. Wijaya IDA AYU ASTARINI Ida Bagus Made Bramasta Wira bumi Ida Bagus Made Bramasta Wirabumi Ida Bagus Wayan Gunam Irawati Irawati Junita Hardini Krisdayani, Putu Mita Luh Putu Melandani Made Ria Defiani Meitini Proborini Wahyuni Muhammad Ibadullah Ni Kadek Sari Dewi Handayani NI KADEK YUNITA SARI Ni Luh Gede Cerli Tonika Sari Ni Luh Putu Ariwathi Ni Luh Putu Rika Sugiantari Ni Luh Suriani Ni Luh Watiniasih NI MADE DENNI APRIANTY Ni Made Dwi Sri Wulandari Ni Made Puspawati Ni Made Suartini NI NYOMAN DARSINI Ni Putu Adriani Astiti Ni Putu Ariwathi Ni Putu Muni Raisani Ni Putu Sri Risa Dewi Ni Putu Yuni Astriani Dewi Ni Wayan Ayu Riantini Pande Ketut Sutara PetroneLa Deno Raja Pramesti, Ni Kadek Rika Putu Suwardike Putu Yayun Antari Budaya Rindang Dwiyani Sista Nirmala Wenni Setyo Lestari, Wenni Setyo Wijaya, I Made Saka Wira bumi, Ida Bagus Made Bramasta Zaenul Gafari

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