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Journal : METAMORFOSA Journal of Biological Sciences

PENURUNAN FUNGSI HATI TIKUS BETINA (Rattus norvegivus L) YANG DIINJEKSI WHITE VITAMIN C DOSIS TINGGI DALAM JANGKA WAKTU LAMA DITINJAU DARI KADAR SGPT, SGOT SERTA GAMBARAN HISTOLOGI HATI Ni Wayan Sudatri; Iriani Setyawati; Ni Made Suartini; Dwi Ariani Yulihastuti
Metamorfosa: Journal of Biological Sciences Vol 3 No 1 (2016)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2016.v03.i01.p07

Abstract

This study aims to determine the side effects of high-dose injections of vitamin C in a long term against liver histology. This study used a completely randomized design (CRD) with different long treatments of vitamin C injection in various doses of P0 (control), P1 (30 days injection), P2 (50 days injection), P3 (70 days injection) and P4 (90 days injection). Parameters measured were plasma levels of SGPT and SGOT, the percentage of hydropic degeneration,  fatty degeneration, pyknotik nuclei, and necrosis of liver cells, and also inflammatory cell infiltration and sinusoid congestion. SGOT levels showed a significant differences (P=0,009) between the treatment and control group by ANOVA test. The Kruskal Wallis test showed that ALT levels were not significantly different (P=0,86), however, the levels of creatinine showed significant differences (P=0,002). The hydropic degeneration were not significantly different (P=0,054) by Kruskal Wallis test, whereas the fatty degeneration were significantly different (P=0,000) between the treatment and control groups by ANOVA test. Pyknotik nuclei and necrotic cells showed significant differences between the control and treatment with the P value of 0,016 and 0,01 respetively. The injected white vitamin C in high doses for long periods decreased the physiological functions of female mice liver.
DIVERSITAS SERANGGA PADA PERKEBUNAN PEPAYA (Carica papaya L.) DI DAERAH SANUR, DENPASAR, BALI Ni Made Suartini; Ni Wayan Sudatri; Ni Luh Watiniasih
Metamorfosa: Journal of Biological Sciences Vol 2 No 2 (2015)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The role of insects in papaya plants is as pollinators, pests, parasites and predators of other insects. Referring to the role of insects, this research was conducted to determine the insects on an papaya plantations. Traping method using methyl eugenol and ripe papaya fruit as well as the manual method with insect net used in this research. Each method was repeated three times with an interval of sampling is one week. Insect netting done on the same day with traping method. Samples were preserved and then taken to the laboratory for identification. Environmental factors were measured are: temperature, humidity, latitude and altitude. Insects were identified by reference: Borror et al. (1992), CSIRO I & II (1991) and also Peggie and Amir (2006). Five species belonging to the genus Bactrocera found on methyl eugenol traps and five species belonging to the genus Drosophila was found in ripe papaya fruit traps as well as 16 other species found using insect netting around the papaya plant. In addition, there is also the genus Paracoccus on a papaya plant. Bactrocera papayae and genus Paracoccus is an insect pest on papaya plants.Keywords: diversity, insect, papaya plantation, Sanur
Diversitas Gastropoda pada Habitat Persawahan dengan Ketinggian Berbeda Ni Made Suartini; Ni Wayan Sudatri
Metamorfosa: Journal of Biological Sciences Vol 6 No 2 (2019)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2019.v06.i02.p11

Abstract

Gastropods or often called snails, live in various habitats including in rice fields habitat. There are several species of rice field gastropods which are detrimental because they damage the plants but some can be utilized. The presence of rice field gastropods is influenced by environmental factors. Environmental factors are certainly different at different altitudes, so that it might affect the species of Gastropods that exist. This study aims to determine the diversity of Gastropods found on rice fields at different altitude. Gastropod sampling were carried out in several rice fields with different altitudes, that is under 100 m, 100 m to 300 m and above 300 m to 500 m above the sea level. Sampling were done by making a 1mx1m square placed in each corner of the rice field and in the middle of the rice field. In addition to the rice fields, samples were also taken from irrigation channels that irrigated the fields. Gastropods found were six species where Lymnaea rubiginosa and Digoniostoma truncatum were only found in rice fields in each location and Terebia granifera was only found in irrigation channels. Melanoides tuberculata, Melanoides maculata and Pomacea canaliculata were found in rice fields and irrigation channels. Locations I and II have a moderate diversity index (1,360 and 1,408) and location III is low (0,795).
Prevalensi Leucocytozoonosis Dan Plasmodiosis Pada Itik (Anas plathyrynchos) Yang Dipelihara Dalam Skala Rumah Tangga Kadek Indah Kartika Sari; Ni Wayan Sudatri; Ni Made Suartini
Metamorfosa: Journal of Biological Sciences Vol 8 No 1 (2021)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2021.v08.i01.p06

Abstract

Leucocytozoon and Plasmodium are blood parasitic protozoa that can be found in poultry blood including ducks. These protozoa can cause Leucocytozoonosis and Plasmodiosis in poultry. Spread of the disease through different biological vectors. Similium sp. and Culicoides arakawae as vectors of Leucocytozoonosis and vectors of Plasmodiosis are Culex sp., Culiseta sp., and Aedes sp. This study aims to determine the prevalence of Leucocytozoonosis and Plasmodiosis in ducks that are maintained on a household scale. Ducks are taken from four locations (breeders on a household scale) in Besan Village. In each location, 5 ducks were taken so that the total number of ducks used was 20. This research used purposive sampling method. Samples of duck blood were made as blood smear preparations and stained with 10% Giemsa stain solution then observed under a microscope. The parameters observed were the percentage of blood samples infected with Leucocytozoon and Plasmodium and hematological parameters (total leukocyte count, total erythrocyte count, hemoglobin level, and PCV value). The results showed that Leucocytozoon and Plasmodium were not found in duck blood cells. The prevalence of Leucocytozoonosis and Plasmodiosis in ducks was 0%. The average value of duck blood profile through One Way Anova statistical analysis showed no significant difference between ducks from each sampling location (p> 0.05) and the value of duck blood profile for all samples was within the normal range.
Penurunan Kualitas Sperma Tikus (Rattus novergivus) yang Diinjeksi Vitamin C Dosis Tinggi dalam Jangka Waktu Lama Ni Wayan Sudatri; Dwi Ariani Yulihastuti; Ni Made Suartini
Metamorfosa: Journal of Biological Sciences Vol 6 No 1 (2019)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2019.v06.i01.p02

Abstract

Benefits of white vitamin C to increace the immune system is already exis. However, the use of high doses of vitamin C and in the long term can cause side effects.. The objective of this study was to determine the side effects of high-dose injections of vitamin C in the long term on sperm quality of rats. The design used in this study is completely randomized design (CRD) with long treatment injections of high doses of vitamin C are different. ie: P0 (control), P1 (injected 30 days), P2 (injected 50 days ), P3 (injected 70 days) and P4 (injected 90 days). Parameters measured were sperm quality (motility, viability, morphology, number) ,. The results showed that the sperm quality of rats (Rattus novergivus). Sperm quality of male rats (Rattus norvegivus L.) were injected vitamin C in high doses for long periods of time between the control and treatment groups showed significant differences (P = 0.000). The quality of sperm of mice injected high doses of white vitamin C tend to decrease sperm quality indicated by percentage of motility, viability, and morphology of spermatozoa although the concentration of spermatozoa tends to increase. Keywords: white vitamin C, rat, sperm quality
Biodiversitas Capung Di Desa Petulu, Kecamatan Ubud, Kabupaten Gianyar, Bali Anak Agung Gde Raka Dalem; Job Nico Subagyo; Ni Made Suartini
Metamorfosa: Journal of Biological Sciences Vol 9 No 2 (2022)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2022.v09.i02.p13

Abstract

ABSTRACT Rearch on “Biodiversity of Dragonflies in Petulu Village, Ubud District, Gianyar Regency, Bali” was undertaken between May and October, 2020. Objectives of this research were to find out species and biodiversity of dragonflies of Petulu Village. Sample were collected from some research locations representing different habitats, such as rice fields, settlements, and plantations. Dragonflies which were caught or sighted on research area whould be caught by insect nests when possible and then were brought to the laboratorium at Biology Department-Fmipa Udayana University, Bali for species identification. Identification was conducted refering to some books and manuals, such as Wahyu Sigit Rhd (2013), Lilies S. (1992), etc. Data of dragonflies that were recorded including name of species and their relative abundances, and then the results were presented on a table. The status of each species (protected or not), was also provided. Shannon-Wiener diversity indexes were calculated. Results of this research showed that there were 12 species of dragonflies found, namely: Crocothemis servilia, Pantala flavescens, Orthetrum Sabina, Neurothemis terminata, Diplacodes trivialis, Potamarcha congener, Neurothemis ramburii, Orthetrum chrysis, Ischnura senegalensis, Agriocnemis femina, Agrionoptera insignis, dan Agriocnemis pygmaea. All dragonflies species found here were not protected by law. Biodiversity index (H’) of dragonflies in Petulu Village was 1.63, which indicated condition of community structure of dragonflies in Petulu Village was stable within the category of moderate. Keywords: Bali, biodiversity, dragonfly, identification, Petulu