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Pengaruh Fermentasi Tongkol Jagung dengan Ragi Tape Komersial terhadap Kandungan Serta Kecernaan Selulosa, Hemiselulosa dan Lignin Secara In Vitro: The Effect of Corn Cob Fermentation With Commercial Tape Yeast on Content And Digestability of Cellulose, Hemicellulose and Lignin In Vitro Ivony Enjelita Oktavia Lakapu; Twenfosel Ocsierly Dami Dato; Yelly Magdalena Mulik
Jurnal Peternakan Lahan Kering Vol. 4 No. 3 (2022): September
Publisher : Universitas Nusa Cendana

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Abstract

Tujuan dari penelitian ini adalah untuk mengetahui pengaruh fermentasi tongkol jagung dengan ragi tape komersial terhadap kandungan dan kecernaan in vitro selulosa, hemiselulosa, dan lignin. Metode yang digunakan dalam penelitian ini adalah metode eksperimen menggunakan Rancangan Acak Lengkap (RAL) dengan 5 perlakuan dan 3 ulangan. Perlakuan tersebut: R-25 (tongkol jagung + aditif + 25g ragi tape); R-50 (tongkol jagung + aditif + 50g ragi tape); R-75 (tongkol jagung + aditif + 75g ragi tape); R-100 (tongkol jagung + aditif + 100g ragi tape); dan R-125 (tongkol jagung + aditif + 125g ragi tape). Variabel yang diamati adalah kandungan serta kecernaan in vitro selulosa, hemiselulosa, dan lignin. Data dianalisis menggunakan sidik ragam dan uji lanjutan dengan uji berganda Duncan. Hasil penelitian  menunjukkan  perlakuan fermentasi tongkol jagung dengan ragi tape komersial tidak mempengaruhi (P>0,05) terhadap kandungan selulosa dan hemiselulosa, namun mempengaruhi (P<0,05) terhadap kandungan lignin serta kecernaan in vitro selulosa, hemiselulosa dan lignin dengan pilihan terbaik pada level penggunaan ragi tape komersil 125 gram. Disimpulkan bahwa semakin tinggi pemberian dosis ragi tape R-125 g/2 kg tongkol jagung akan menurunkan kandungan selulosa, hemiselulosa dan lignin dan meningkatkan kecernaan in vitro selulosa, hemiselulosa dan lignin pada tongkol jagung. The purpose of this study was to determine the effect of fermenting corncobs with commercial tape yeast on the content and digestibility of in vitro cellulose, hemicellulose, and lignin. The method used in this study is an experimental method using a completely randomized design (CRD) with 5 treatments and 3 replications. The treatment: R-25 (corn cobs + additives + 25g yeast tape); R-50 (corn cobs + additives + 50g yeast tape); R-75 (corn cobs + additives + 75g yeast tape); R-100 (corn cobs + additives + 100g yeast tape); and R-125 (corn cobs + additives + 125g yeast tape). The variables observed were the content and in vitro digestibility of cellulose, hemicellulose, and lignin. Data were analyzed using variance and further test with Duncan's multiple test. The results showed that the fermentation treatment of corn cobs with commercial tape yeast did not affect (P>0.05) on the cellulose and hemicellulose content, but did affect (P<0.05) on the lignin content and in vitro digestibility of cellulose, hemicellulose and lignin with the best choice. at the level of the use of commercial tape yeast 125 grams. It was concluded that the higher dose of tape yeast R-125 g/2 kg corn cobs would decrease the content of cellulose, hemicellulose and lignin and increase in vitro digestibility of cellulose, hemicellulose and lignin in corn cobs.
PENGARUH RASIO KARBON-NITROGEN DALAM ENSILAGE CAMPURAN MUKUNA LOKAL (MUCUNA SP) DAN RUMPUT KUME (SORGHUM PLUMOSUM VAR. TIMORENSE) SEGAR TERHADAP KANDUNGAN NUTRISI PRODUK (The effects of carbon-nitrogen ratio in ensilage of local mucuna (mucuna sp.) And... Vinensius Darmin; Twenfosel O. Dami Dato; Marthen Luther Mullik
JURNAL NUKLEUS PETERNAKAN Vol 9 No 2 (2022): Desember
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/nukleus.v9i2.7835

Abstract

The main problem in preservation of protein source forages in silage making is spoilage due to high buffering characteristic of protein. This might relates to low carbon-nitrogen (C-N) ratio in these plants. This study aimed at determining the best level of C-N ratio in ensilage process of a grass-legume mixture. A 3x4 completely randomized design (CRD) was deployed to test three C-N ratio levels as treatments. The treatments were CN20 (Mucuna sp + kume grass + rice bran to reach C-N ratio of 20, or 30 (CN30) or 40 (CN40). Variables measured were dry matter (DM), organic matter (OM), crude protein (CP), ether extract (EE), and crude fiber (CF) content of the silage. Data were analyzed using General Linear Model for CRD with an alfa value of 0.05. Treatment differences were determined using Duncan test. The results showed that C-N ratio had high significant effects on DM content (ranged from 22.5 to 32.0%), and CP content (ranged from 7.5 to 9.6%). Significant treatment effects were detected in OM content (ranged from 90.5 to 91.8%), and EE content (ranged from 2.82 to 3.66%). Meanwhile, CF was not effected by C-N ratio (ranged from 39.0 to 3.0%. 44.2%). It could be concluded that C-N the ratio of 30 is the best treatment in silage making using mixture of lelehanak-kume grass as it shows the highest OM content and had no significant difference with CN20 in CP content.
Levels of Tape Yeast in Corn Cob Bioconversion Affects Crude Protein Content and In Vitro Digestibility of Crude Fiber Marthen L. Mullik; Sabneno Yuliana Sabneno; Marthen Yunus; Twen O. Dami Datto
Pastura : Jurnal Ilmu Tumbuhan Pakan Ternak Vol 12 No 1 (2022): Pastura Vol. 12 No. 1 Tahun 2022
Publisher : Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/Pastura.2022.v12.i01.p07

Abstract

Penelitian bertujuan untuk mengevaluasi pengaruh ragi tape dalam proses biokonversi terhadap kandungan dan kecernaan in vitro protein kasar (PK) dan serat kasar (SK) produk biokonversi tongkol jagung. Metode eksperimen ini menggunakan rancangan acak lengkap (RAL) dengan lima perlakukan dan tiga ulangan untuk menguji lima level pemberian ragi tape dalam biokonversi tongkol jagung yaitu 2 kg cacahan tongkol jagung ditambah dan ragi tape masing-masing 25 g (RT25), 50 g (RT50), 75 g (RT75), 100 g (RT100) dan 125 g (RT125). Hasil penelitian menunjukkan bahwa kandungan PK berkisar 10,16-11,74% yang dipengaruhi oleh level ragi tape (P=0.046). Sebaliknya, kandungan SK dan kecernaan PK tidak dipengaruhi oleh level ragi tape. Tingkat kecernaan total SK secara nyata menurun dari 26,35% ke 22,78% seiring peningkatan level ragi tapi. Dapat disimpulkan bahwa penggunaan ragi tape sebesar 75 g per 2 kg tongkol jagung merupakan level terbaik dalam biokonversi karena menghasilkan kandungan protein tertinggi, sedangkan kandungan serat kasar rendah. Kata kunci: protein, serat kasar, kecernaan in vitro, ragi tape, tongkol jagung
Kandungan dan Kecernaan Bahan Kering dan Bahan Organik Lamtoro Tarramba (Leucaena leucocephala cv. Tarramba) yang Diberi Pupuk Amazing Bio Growth Tablet dengan Level yang Berbeda Secara In Vitro: Content and Digestibility of Dry Matter and Organic Matter Lamtoro Tarramba (Leucaena leucocephala cv. Tarramba) Given Fertilizer Amazing Bio Growth Tablets With Different Level In Vitro Rio Anderias Sasongko Sakan; Twenfosel Ocsierly Dami Dato; Marthen Yunus
Jurnal Peternakan Lahan Kering Vol. 5 No. 1 (2023): Maret
Publisher : Universitas Nusa Cendana

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Abstract

This study aims to determine the effect of the use of organic fertilizers Amazing Bio Growth tablet with different levels of content and digestibility in vitro forage of lamtoro tarramba. The experimental design used in this study was a completely randomized design, consisting of 5 treatments with 3 replications. Such treatment is: R₀= without fertilizer ABG tablet   (control), R₁= ABG tablets 10g, R₂= ABG tablet 20g, R₃ = ABG tablet 30g, and R₄ = ABG tablet 40g. The variables observed were: content and digestibility of dry matter and organic matter in vitro. The results showed the mean dry matter content(%) : R₀ (88.53±0.22); R₁ (88.77±0.60); R₂ (88.86±0.25); R₃ (88.91±1.47); R₄ (89.64±0.74); organic matter content: R₀ (80.51±0.10); R₁ (80.695±0.47); R₂ (81.60±0.33); R₃ (81.69±1.29); R₄ (81.98±0.46); digestibility in vitro of dry matter (%): R₀ (71.39±0.72); R₁ (72.24±0.09); R₂ (72.43±1.15); R₃ (73.91±2.26); R₄ (74.57±1.12); and digestibility in vitro of organic matter (%): R₀ (66.15±1.63); R₁ (66.18±1.88); R₂ (67.70±0.86); R₃ (67.91±2.70); R₄ (70.35±0.49). The results of statistical analysis showed that the treatment of ABG tablet organic fertilizer had no significant effect (P> 0.05) on the content and digestibility in vitro of dry matter and organic matter. It was concluded that the use of ABG tablet organic fertilizer was 40g significantly. can reduce the content of dry matter and organic matter so that it gives a real response to the digestibility in vitro of organic matter and dry matter in tarramba lamtoro forage. Penelitian ini bertujuan untuk mengetahui pengaruh penggunaan pupuk organik Amazing Bio Growth tablet dengan level yang berbeda terhadap kandungan dan kecernaan in vitro bahan kering dan bahan organic hijauan lamtoro tarramba. Rancangan percobaan yang digunakan adalah rancangan acak lengkap, terdiri dari 5 perlakuan dengan 3 ulangan, yakni: R₀ = tanpa pupuk ABG tablet (kontrol); R₁ = ABG tablet 10g ; R₂ = ABG tablet 20g ; R₃ = ABG tablet 30g ; dan R₄ = ABG tablet 40g. Variabel yang diamati adalah: kandungan dan kecernaan bahan kering dan bahan organik in vitro. Hasil penelitian menunjukkan bahwa rataan kandungan bahan kering (%): R₀ (88,53± 0,21); R₁ (88,77± 0,60); R₂ (88,86± 0,25); R₃ (88,91± 1,46); R₄ (89,64± 0,73); kandungan bahan organic (%): R₀ (80,51±0,11); R₁ (80,69± 0,47); R₂ (81,60± 0,33); R₃ (81,69±1,29); R₄ (81,98±0,46); kecernaan in vitro bahan kering (%): R₀ (71,39±0,72); R₁ (72,24±0,08); R₂ (72,43±1,14); R₃ (73,91±2,25); R₄ (74,57±1,11); dan kecernaan in vitro bahan organic (%): R₀ (66,15±1,63); R₁ (66,18±1,87); R₂ (67,70±0,85); R₃ (67,91±2,70); R₄ (70,35±0,49). Hasil analisis statistik menunjukkan bahwa perlakuan penggunaan pupuk organik ABG tablet berpengaruh tidak nyata (P>0,05) terhadap kandungan dan kecernaan in vitro bahan kering dan bahan organik, Disimpulkan bahwa penggunaan pupuk organik ABG tablet hingga level 40g belum memberikan respon yang nyata terhadap kandungan dan kecernaan in vitro bahan kering dan bahan organik pada hijauan lamtoro tarramba.  
Kualitas Bakar Briket Bioarang Campuran Arang Kotoran Kambing dan Tempurung Buah Lontar (Saboak) dengan Level Perekat Berbeda: Burning Quality of Biocharcoal Briquettes Mixed of Goat Dung and Lontar Shell (Saboak) with Different Levels of Adhesive Ervan Imanuel Taklal; Yakob Robert Noach; Twenfosel Ocsierly Dami Dato
Jurnal Peternakan Lahan Kering Vol. 5 No. 1 (2023): Maret
Publisher : Universitas Nusa Cendana

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Abstract

The experiment goals was to determine burning quality of biocharcoal briquette mixed of goat dung and saboak shell with different levels of adhesive. Materials used consist of 8 kg of goat dung charcoal, 8 kg of saboak shell, 1.5 kg tapioca. The completely randomized design (CRD) consist of 4 treatments and 4 replications was applied. Those treatments were level of adhesive were P1: 6%, P2: 8%, P3: 10% and P4: 12%. The proporsion of both goad dung and saboak shell is 500 gram Variables observed were burning temperature, combustion rate, burning resistance, color and smoke and water boiling ability. Data were analyzed by analysis of variance (ANOVA). The statistical results showed that treatment have significant effect (P<0.05) on burning temperature but no significant (P>0.05) on combustion rate, burning resistance, color and smoke and boiling water ability. Burning temperature ranged 154.17 to 214.09oC; combustion rated ranged 2.14 to 2.44 g/minute; burning resistance ranged 164 to 188 minute; score of color 2.8 to 4 (tend to red until bluish) and score of smoke 3.85 to 4 (without smoke) and boiling water ability 19.5 to 27.75 minute. It can be concluded the increasing of adhesive levels decrease burning temperature, but no change on combustion rate, burning resistance, color and smoke and water boiling ability. The best burning quality of biocharcoal briquettes was founded at P1 that is 6% adhesive (P1). Penelitian bertujuan mengetahui kualitas bakar briket bioarang campuran arang kotoran kambing dan tempurung saboak dengan level perekat berbeda. Materi yang digunakan adalah kotoran  kambing 8 kg, tempurung saboak 8 kg, tapioka 1,5 kg dan air. Metode yang digunakan yaitu Rancangan Acak Lengkap (RAL) dengan 4 perlakuan dan 4 ulangan. Perlakuan level perekat dari bahan bioarang yaitu P1: 6%, P2: 8%, P3: 10%, dan P4: 12%; proporsi kotoran kambing dan tempurung  saboak masing-masing 500 gram. Variabel yang diteliti: temperatur bakar, laju pembakaran, ketahanan bakar, warna dan asap serta kemampuan mendidihkan air. Data dianalisis dengan analisis keragaman (ANOVA). Hasil analisis menunjukkan perlakuan berpengaruh nyata (P<0,05) terhadap temperatur bakar, berpengaruh tidak nyata (P>0,05) terhadap laju pembakaran, ketahanan bakar, warna dan asap serta kemampuan mendidihkan air. Temperatur bakar briket bioarang yang didapatkan berkisar 154,17-214,09oC; laju pembakaran 2,14-2,44g/menit; ketahanan bakar 164-188 menit; skor warna pembakaran 2,8-4 (cenderung merah - merah kebiruan) dan skor asap 3,85-4 (cenderung tidak ada asap - tidak ada asap) dan kemampuan mendidihkan air 19,5-27,75 menit. Disimpulkan bahwa naiknya level perekat menurunkan temperatur bakar tapi tidak menyebabkan perubahan laju pembakaran, ketahanan bakar, warna dan asap pembakaran serta kemampuan mendidihkan air. Kualitas bakar terbaik didapatkan pada P1 yaitu penggunaan level perekat 6%.  
Pengaruh Lama Waktu Biofermentasi Chromolaena odorata dengan Sumber Karbon Gula Lontar Cair Terhadap Konsentrasi pH, VFA Total, NH3 dan Produksi Gas Metan Secara in vitro Tamara Petan; Gustaf Oematan; Twen O Dami Dato; Gusti Ayu Y. Lestari
Animal Agricultura Vol 1 No 2 (2023): Animal Agricultura
Publisher : Yayasan Sumber Daya Manusia Cerdas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59891/animacultura.v1i2.19

Abstract

The purpose of this study was to determine the effect of Chromolaena odorata biofermentation time with liquid palm sugar carbon source on pH concentration, total VFA, NH3 and methane gas production in vitro. The method used in this research is an experimental method using a completely randomized design (CRD) with 4 treatments and 4 replicates. The treatments were LB21: 21 days of biofermentation as control, LB14: 14 days of biofermentation, LB7: 7 days of biofermentation, LB0: 0 days of biofermentation. The observed variables were pH, total VFA, NH3, and methane gas production. The results of statistical analysis showed that Chromolaena odorata biofermentation with liquid palm sugar carbon source had a significant effect (P<0.05) on pH and NH3 concentrations while total VFA and methane gas production had no significant effect (P>0.05). It is concluded that the length of biofermentation time affects pH and NH3 while not affecting total VFA and methane gas production. Biofermentation time of 14 days gave the best pH value while 7 days gave the best NH3.
Pengaruh Lama Fermentasi Dedak Gandum Terhadap Kandungan Serat Kasar, NDF dan ADF Mesang, Sarintan Novite I.; Oematan, Gustaf; Mullik, Marthen L.; Dami Dato, Twen O
Animal Agricultura Vol 2 No 2 (2024): Animal Agricultura
Publisher : Yayasan Sumber Daya Manusia Cerdas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59891/animacultura.v2i2.84

Abstract

This study aims to determine the effect of the duration of wheat bran fermentation on the content of crude fiber, neutral detergent fiber (NDF), and acid detergent fiber (ADF). The design used was the Completely Randomized Design (CRD) method with 4 treatments and 4 replications, resulting in 16 experimental units. The treatments consisted of fermentation durations of 0 days (LF0), 2 days (LF2), 4 days (LF4), and 6 days (LF6). Prior to fermentation, wheat bran was mixed with an inoculum solution at a 1:1 ratio. The inoculum was made from a mixture of 16 liters of clean water, 30 ml of EM4, 100 ml of liquid sugar, and 100 ml of fresh cow feces liquid taken from cows fed with lamtoro and fresh grass. The variables studied in this research were the content of crude fiber, neutral detergent fiber (NDF), and acid detergent fiber (ADF). The data were analyzed using analysis of variance (ANOVA) for CRD where the alpha value was set at 0.5%, and differences between treatments were followed by Duncan's test using SPSS version 25. The results of the statistical analysis showed that the duration of wheat bran fermentation had a very significant effect (P<0.01) in reducing all fiber variables. The total value of crude fiber content decreased from 51.95% (LF0) to 42.86% (LF6), likewise NDF decreased from 36.55% (LF0) to 29.90% (LF6), and ADF content decreased from 13.62% (LF0) to 9.12% (LF6). Based on the results of this study, it can be concluded that the longer fermentation of wheat bran using fresh cow feces liquid as an inoculum is capable of reducing the content of crude fiber, NDF, and ADF with a fermentation duration of 6 days (LF6), obtaining total values of crude fiber content (42.86%), NDF (29.90%), and ADF (9.12%).
Pengaruh Level Campuran Tepung Daun Kelor-Lamtoro dalam Media Dasar Terhadap Pemanfaatan dan Konversi Nutrisi Maggot Lalat Tentara Hitam (Hermetia illucens) Rendemai, Isidorus; Dami Dato, Twen O.; Benu, Imanuel; Oematan, Gustaf
Animal Agricultura Vol 2 No 2 (2024): Animal Agricultura
Publisher : Yayasan Sumber Daya Manusia Cerdas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59891/animacultura.v2i2.90

Abstract

leaf meal mixture in the basal medium on the utilization of dry matter, crude protein, crude fat, and nutrient conversion in black soldier fly larvae (BSFL). The experiment was designed using a completely randomized design (CRD) with five treatments (0%, 10%, 20%, 30%, and 40% of the Moringa-Leucaena leaf meal mixture) and four replications. The basal medium consisted of 80% rice bran and 20% cornmeal, fermented for seven days before use. A total of 1000 BSFL were introduced into the fermented medium in each experimental unit, and harvesting was conducted on day 14.The results showed that the addition of the Moringa-Leucaena leaf meal mixture significantly increased dry matter utilization (DMU: 0.17–0.33%), crude protein utilization (CPU: 1.21–1.62%), and crude fat utilization (CFU: 4.00–7.00%), while reducing the feed conversion ratio (FCR: 6.23–3.27). The optimal level for all variables was found in the treatment with 40% Moringa-Leucaena leaf meal mixture. In conclusion, the addition of Moringa-Leucaena leaf meal as a protein source in the basal medium can improve nutrient utilization and feed efficiency in black soldier fly larvae.
Pengaruh Lama Waktu Biofermentasi Chromolaena Odorata dengan Sumber Karbon Tepung Putak terhadap Kandungan Serta Kecernaan Protein Kasar dan Lemak Kasar In vitro Nomleni, Meri; Dami Dato, Twen O.; Lestari, Gusti A. Y.; Mullik, Marthen L.
Animal Agricultura Vol 2 No 3 (2025): Animal Agricultura
Publisher : Yayasan Sumber Daya Manusia Cerdas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59891/animacultura.v2i3.108

Abstract

The purpose of this study was to determine the effect of the length of biofermentation time of chromolena odorata with a carbon source of putak flour on the content and digestibility of crude protein and crude fat in vitro. The research method used is an experimental method (experimental) with a Complete Random Design pattern consisting of 4 treatments and 4 repeats so that there are 16 experimental units. The treatment used is LB21: biofermentation duration 21 days (As control), LB14: biofermentation duration 14 days, LB7: biofermentation duration 7 days, LB0: biofermentation duration 0 days. The data were obtained and analyzed by variety analysis (ANOVA), and continued with the Duncan multiple distance test to determine the effect between treatments. The results of statistical analysis showed that the length of biofermentation of Chromolaena odorata with the carbon source of putak  flour had a real effect (P<0,01) On crude protein content, crude fat content and crude protein digestibility in vitro, while on crude fat digestibility in vitro the effect is not real (P<0,05). It can be concluded that the length of biofermnetation of Chromolaena odorata with putak flour source affects crude protein content, crude fat and gauze protein digestibility in vitro but does not affect crude fat digestibility in vitro.  
Pengaruh Lama Waktu Biofermentasi Chromolaena Odorata dengan Sumber Karbon Gula Lontar Cair terhadap Kandungan serta Kecernaan In Vitro Bahan Kering dan Bahan Organik Kurnia; Dami Dato, Twenfosel O.; Benu, Imanuel
Animal Agricultura Vol 2 No 3 (2025): Animal Agricultura
Publisher : Yayasan Sumber Daya Manusia Cerdas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59891/animacultura.v2i3.112

Abstract

This study aimed to analyze the effect of biofermentation duration of Chromolaena odorata using liquid palm sugar as a carbon source on the content and in vitro digestibility of dry matter (DM) and organic matter (OM). A Completely Randomized Design (CRD) with four treatments (LB-0 = 0 days, LB-7 = 7 days, LB-14 = 14 days, LB-21 = 21 days) and four replications was applied. Each treatment included liquid palm sugar (C/N ratio of 30) and 5% rumen fluid as a starter inoculum. Observed variables included DM content, OM content, in vitro DM digestibility (IVDMD), and in vitro OM digestibility (IVOMD). Data were analyzed using ANOVA and Duncan’s test (P<0.05). Results showed the highest DM content in LB-14 (91.64%), OM content in LB-7 (90.47%), IVDMD in LB-21 (72.55%), and IVOMD in LB-21 (71.13%). Statistical analysis confirmed that biofermentation duration significantly affected all parameters (P<0.05). The study concluded that the optimal durations were 14 days for DM content, 7 days for OM content, and 21 days for in vitro DM and OM digestibility. This research recommends using fermented Chromolaena odorata as ruminant feed, with fermentation duration adjusted to specific nutritional goals.