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All Journal AGRIVITA, Journal of Agricultural Science Journal of Tropical Biodiversity and Biotechnology Jurnal Ilmiah Biosaintropis (Bioscience-Tropic) Jurnal Kimia Riset Biosaintifika: Journal of Biology & Biology Education Jurnal Ilmiah Mandala Education (JIME) Darmabakti Cendekia: Journal of Community Service and Engagements Syntax Idea Sains dan Matematika Journal Pharmasci (Journal of Pharmacy and Science) Proceeding Biology Education Conference Prosiding Konferensi Nasional PKM-CSR Jurnal Kreativitas dan Inovasi (Jurnal Kreanova) Sewagati Prosiding Seminar Nasional Pengabdian kepada Masyarakat (SINAPMAS)
Junairiah Junairiah
Faculty Of Science And Technology, Universitas Airlangga
Aerospace Engineering Agriculture, Biological Sciences & Forestry Arts Humanities Astronomy Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Physics Public Health Social Sciences Other

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PENGARUH VARIASI ZAT PENGATUR TUMBUH IAA, BAP, KINETIN TERHADAP METABOLIT SEKUNDER KALUS SIRIH HITAM (Piper betle L. Var Nigra) Junairiah Junairiah; Nurul Sofi Amalia; Yosephine Sri Wulan Manuhara; Ni’matuzzahroh Ni’matuzahroh; Lilis Sulistyorini
Jurnal Kimia Riset Vol. 4 No. 2 (2019): Desember
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jkr.v4i2.16898

Abstract

AbstrakPiper betle L. var Nigra (sirih hitam)  merupakan salah satu jenis tanaman obat yang berpotensi untuk dikembangkan dengan metode kultur jaringan. Penelitian ini bertujuan untuk mengetahui pengaruh variasi zat pengatur tumbuh Indole Acetic Acid (IAA), Benzyl Amino Purine (BAP), Kinetin terhadap senyawa metabolit sekunder  yang terdapat kalus sirih hitam. Eksplan daun dari sirih hitam ditanam pada medium Murashige dan Skoog dengan 4 perlakuan kombinasi (I1,0K1,0; I1,0K1,5; I1B1,5; I0,5B0,5). Kalus dipelihara selama 8 minggu. Simplisia kalus sirih hitam di maserasi menggunakan pelarut metanol dan diidentifikasi menggunakan Gas Chromatography Mass Spectra (GCMS). Berdasarkan analisis GCMS menunjukkan bahwa senyawa metabolit sekunder dengan presentase tertinggi terdapat pada masing- masing perlakuan yaitu senyawa gamma sitosterol (14,88%) pada perlakuan I1,0K1,0 ; senyawa 14-Beta H Pregna (15,94%) pada perlakuan I1,0K1,5 serta senyawa beta-d-glucopyranoside (15,54%) pada perlakuan I0,5B0,5; dan senyawa beta-d-glucopyranoside (5,63%) pada perlakuan I1,0 B1,5. Katakunci: Kalus, Piper betle L. var Nigra, senyawa metabolit sekunder  AbstractPiper betle L. Var Nigra (black betel) is one type of medicinal plant that has the potential to be developed by tissue culture method. This study was aims to determine the effect of variations in growth regulator Indole Acetic Acid (IAA), Benzyl Amino Purine (BAP), and Kinetin on secondary metabolite compounds contained in black betel callus. The explant of black betel’s leaf was cultured on Murashige and Skoog medium with four combination treatments (I1.0K1.0; I1.0K1.5; I1B1.5; I0.5B0.5). The callus was maintained for eight weeks. Simplisia of black betel’s callus was macerated using methanol solvent and identified using Gas Chromatography Mass Spectra (GCMS). GCMS analysis showed that secondary metabolites with the highest percentage in each treatment were gamma sitosterol compound (14.88%) in treatment I1.0K1.0; 14-Beta H Pregna compound (15.94%) in I1.0K1.5 treatment and beta-d-glucopyranoside compound (15.54%) in I0.5B0.5 treatment; and beta-d-glucopyranoside compound (5.63%) in I1.0 B1.5 treatment.Keywords: Callus, Piper betle L. Var Nigra, secondary metabolite compounds
INNOVATION OF MEDICINE PLANTS PROPAGATION WITH VERTICULTURE TECHNIQUES IN EFFORTS TO OVERCOME LAND DYSFUNCTIONAL IN EAST SURABAYA AREA Junairiah Junairiah; Tri Nurhariyati; Listijani Suhargo
Darmabakti Cendekia: Journal of Community Service and Engagements Vol. 2 No. 2 (2020): DECEMBER 2020
Publisher : Faculty of Vocational Studies, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (751.964 KB) | DOI: 10.20473/dc.V2.I2.2020.58-61

Abstract

Background: Keputih Village is located in eastern Surabaya. The boundary of this village is the river. Areas on the river banks are overgrown with wild plants and poorly maintained. One way to overcome the dysfunctional land around the river is by cultivating medicinal plants with verticulture techniques. Purpose: The implementation of this program is to overcome the dysfunctional land around the Keputih Village’s river by cultivating medicinal plants in verticulture techniques and provide training in the manufacture of herbal products from the cultivation of medicinal plants and provide financial management training to the local residents. Methods: This activity was carried out in 4 stages. Phase I was training in making pots and verticulture shelves as well as planting medicinal plants, Phase II was mentoring in the field, Phase III was training on making herbal products from plants and training on simple bookkeeping, Phase IV was product display. Results: Based on the results of this training showed that the knowledge of the participants related to the training material has increased which shown from the results of the posttest with average of 79,31 and product display. Conclusion: In this activity, beside gaining knowledge, participants can apply this knowledge to increase family income.
Pemberdayaan Pemuda Karang Taruna Dan Ibu-Ibu Pkk di Kelurahan Pacar Kembang, Surabaya Junairiah Junairiah; Tri Nurhariyati; Listijani Suhargo
Prosiding Konferensi Nasional Pengabdian Kepada Masyarakat dan Corporate Social Responsibility (PKM-CSR) Vol 3 (2020): Peran Perguruan Tinggi dan Dunia Usaha Dalam Pemberdayaan Masyarakat Untuk Menyongsong
Publisher : Asosiasi Sinergi Pengabdi dan Pemberdaya Indonesia (ASPPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (265.366 KB) | DOI: 10.37695/pkmcsr.v3i0.1047

Abstract

Kelurahan Pacar Kembang terletak di wilayah Surabaya Timur. Permasalahan yang dihadapi oleh mitra adalah terdapat kampung yang kumuh dan belum tertata, juga pemuda karang taruna dan ibu-ibu PKK yang belum mempunyai kegiatan edukasi untuk dapat mewujudkan kampung yang bersih dan asri. Kegiatan ini bertujuan untuk mewujudkan kampung yang bersih, asri dan aman. Berdasarkan permasalahan tersebut di atas, maka permasalahan mitra yang harus segera ditangani adalah aspek lingkungan dan budidaya dengan memberikan pelatihan mural dan budidaya lidah buaya dilanjutkan dengan kegiatan praktek terhadap materi yang diberikan. Berdasarkan hasil evaluasi terhadap materi yang telah disampaikan menunjukkan bahwa rerata nilai pelatihan mural adalah 76 dan rerata nilai pelatihan budidaya lidah buaya adalah 82. Hasil praktek pembuatan mural dan pelatihan budidaya berupa foto kegiatan
Effect of Plant Growth Regulators on Callus Induction in Piper betle L. var. Nigra: Pengaruh Berbagai Jenis ZPT terhadap Induksi Kalus Piper betle L. var. Nigra Junairiah Junairiah; Ni'matuzahroh Ni'matuzahroh; Nabilah Istighfari Zuraidassanaaz; Lilis Sulistyorini
Journal Pharmasci (Journal of Pharmacy and Science) Vol. 8 No. 1 (2023): Journal Pharmasci (Journal of Pharmacy and Science)
Publisher : Akademi Farmasi Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53342/pharmasci.v8i1.302

Abstract

Callus culture is one of the plant tissue culture techniques that used to study aspects of plant nutrition, somatic embryogenesis, cell suspension culture, secondary metabolite production, and genetic transformation. Callus induction was carried by adding growth regulators. Black betel (Piper betle L. var. nigra) is a medicinal plant that has the potential to produce secondary metabolites. The aim of this study was to obtain the best formula for callus induction of P. betle L. var. nigra. The single growth regulator used is 2,4-Dichlorophenoxyacetic acid (2,4-D), Benzyl Amino Purine (BAP), Indole Butyric Acid (IBA), Indole Acetic Acid (IAA), and Naphthalene Acetic Acid (NAA) with variations the concentration used is 0; 0.5; 1; 1.5; 2; 2.5 mg/L. Each treatment consisted of 6 replicates grown using Murashige and Skoog (MS) medium in vitro for eight weeks. The results showed that the 1.5 mg/L 2,4-D treatment produced the highest fresh weight (1389.5 mg) and dry weight (55.7 mg). Callus P. betle L. var. Nigra in various treatments showed various textures such as compact and friable with callus colors such as white, greenish-white, brownish-white, yellowish-green, greenish-yellow, brownish-yellow, greenish-brown, brown, and gray.
Effect of Abiotic and Biotic Elicitors on Callus and Suspension from Piper Betle L. Var. Nigra Junairiah Junairiah; Ni’matuzahroh Ni’matuzahroh; Lilis Sulistyorini; Nabilah Istighfari Zuraidassanaaz
Syntax Idea 434-443
Publisher : Ridwan Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46799/syntax-idea.v5i4.2170

Abstract

Piper betle L. var. Nigra (black betel) contains secondary metabolites and has biological activity as antibacterial, antifungal, antioxidant, etc. To increase the production of secondary metabolites, an alternative method is needed, namely cell suspension culture. This study aims to determine the effect of abiotic and biotic elicitor on callus biomass produced from cell suspension culture. Leaf explants were grown on Murasige and Skoog (MS) medium with the addition of growth regulator 2.4-D 0.5 mg/L and BAP 2.0 mg/L with abiotic elicitors CuSO4, ZnSO4, HgCl2 and CoCl2 with a concentration of 0.5; 1.0 and 2.5 mg/L. The biotic elicitor used was Aspergillus niger with a concentration of 0.025%; 0.050% and 0.1%. The cultures were incubated for 8 weeks. 0.5 g callus was subcultured on 25 mL cell suspension medium. The suspension culture was shaken at 110 rpm. In this suspension culture, the callus was incubated for 3 weeks. After 3 weeks of age callus on suspension medium was harvested and weighed the fresh and dry weight. The results showed that the highest average dry weight was found in the treatment with abiotic elicitor CuSO4 0.5 mg/L at 0.088 g.
Callus Induction of Piper betle L. Var Nigra Using 2,4-Dichlorofenoxyacetic Acidand 6-Benzil Aminopurin Junairiah Junairiah; Purnomo Purnomo; Edy Setiti Wida Utami; Ni’matuzahroh Ni’matuzahroh; Lilis Sulistyorini
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 3 (2018): December 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i3.15962

Abstract

Piper betle L var Nigra (black betel) is a member of Piperaceae family which has potential as medicinal plant due to its secondary metabolites. Callus culture is one of the alternative methods to elevate production of secondary metabolites. This study was aimed to determine the effect of 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzil aminopurine (BAP) towards callus induction and growth, also to determine the most optimal variation of 2,4-D and BAP concentration for callus induction of black betel leaf explant. This study was a laboratory experimental study with complete randomized design. Black betel leaf explant was planted in Murashige and Skoog (MS) medium supplemented with 2,4-D and BAP growth regulators at concentration of 0.0 mg/L, 0.5 mg/L, 1.0 mg/L, 1.5 mg/L, 2.0 mg/L respectively. Parameter recorded for callus induction and growth including callus induction time (days), percentage of explant forming callus, fresh weight, dry weight, color and texture. After callus planted for 8 weeks, analysis was performed statistically. Result showed that 2,4-D and BAP supplementation to medium affected the growth of black bete l leaf explants. Additional concentration of 0.5 mg/L 2,4-D and 1.0 mg/L BAP growth regulators showed the fastest response in callus formation, at 7.25 days. Growth regulators of 2,4-D 0.5 mg/L and BAP 2.0 mg/L concentration produced the highest fresh and dry weight, at 0.6802 g and 0.0670 g respectively. The best treatment was used as a basis to produce secondary metabolites.
Pembuatan Hand Sanitizer, Minuman Herbal dan Budidaya Tanaman Obat untuk Pencegahan Covid-19 Junairiah Junairiah; Listijani Suhargo; Anjar Tri Wibowo
Prosiding Seminar Nasional Pengabdian kepada Masyarakat (SINAPMAS) Perguruan Tinggi Mengabdi: Berkarya dan Berinovasi Untuk Membangun Masyarakat Semakin Tangguh di Mas
Publisher : Prosiding Seminar Nasional Pengabdian kepada Masyarakat (SINAPMAS)

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Pandemi COVID-19, mengharuskan masyarakat untuk membiasakan hidup sehat. Selain dengan memakai masker, masyarakat juga dianjurkan untuk menggunakan hand sanitizer dan mengkonsumsi minuman suplemen secara rutin untuk menjaga dan meningkatkan imunitas tubuh. Saat ini telah beredar berbagai jenis hand sanitizer dan minuman suplemen di pasaran, namun dengan harga yang tidak murah dan kadang langka. Hal ini menyebabkan masyarakat enggan untuk membeli serta menggunakan hand sanitizer dan minuman suplemen. Kegiatan pengabdian masyarakat ini bertujuan untuk melatih mitra supaya dapat membuat hand sanitizer, minuman herbal dan budidaya tanaman obat. Metode yang digunakan terdiri atas empat tahap yaitu pretest, materi tentang pembuatan hand sanitizer, minuman herbal, budidaya jahe dan sereh serta manajemen keuangan. evaluasi berupa postest dan tahap terakhir adalah praktek pembuatan hand sanitizer dari bahan herbal, membuat minuman herbal serta budidaya jahe dan sereh. Hasil dari kegiatan ini menunjukkan bahwa terdapat peningkatan pengetahuan terhadap materi yang telah diberikan, hal ini dapat dilihat dari hasil post test yang menunjukkan peningkatan nilai menjadi 91,11, dari nilai awal pretest 75,33. Ada kenaikan nilai sebesar 20,94%. Kegiatan ini diharapkan dapat membantu pencegahan penyebaran COVID-19.Kata kunci—COVID-19, hand sanitizer, herbal 
Amorphophallus muelleri Blume Shoot Induction on Different Media Types and Plant Growth Regulator Combinations Oktavia, Gebby Agnessya Esa; Junairiah, Junairiah; Manuhara, Yosephine Sri Wulan
AGRIVITA Journal of Agricultural Science Vol 46, No 3 (2024)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v46i3.4477

Abstract

Conventional propagation experiences problems due to long period for seed production, disease contamination, low reproductive rate and season dependent. In vitro propagation offers several advantages. This research aims to assess the impact of different media types and the concentrations of BAP and IBA on the in vitro growth and development of A. muelleri shoots. Bulbil explants were cultivated using MS media with different media types (solid media, agitated liquid media, and static liquid media) with various BAP and IBA concentration treatments (1 mg/l, 2 mg/l, and 3 mg/l). The results demonstrated that the media type and combinations of BAP and IBA treatments significantly influenced period of callus formation and the number of shoots. The development of explant on both solid and static liquid media involves initial explant swelling, formation of nodular callus, greenish buds, and ultimately shoots. Anatomical observation showed that explants experienced indirect organogenesis, characterized by the presence of pre meristem nodular callus, meristematic tissue, apical meristem and leaf primordia. The agitated liquid media didn’t promote growth and development of the explants. The most effective combination was found to be the static liquid media supplemented with 3 mg/l BAP and 1 mg/l IBA.
Pelatihan Pembuatan dan Pemberian Probiotik dan Single Cell Protein pada Sapi Perah di Komunitas Peternak Junrejo, Kota Batu Amin, Muhammad Hilman Fu'adil; Wibowo, Anjar Tri; Supriyanto, Agus; Pratiwi, Intan Ayu; Wahyuningsih, Sri Puji Astuti; Pramudya, Manikya; Wahyuni, Dwi Kusuma; Junairiah, Junairiah; Dewi, Firli Rahmah Primula; Geraldi, Almando
Sewagati Vol 8 No 6 (2024)
Publisher : Pusat Publikasi ITS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j26139960.v8i6.2340

Abstract

Komunitas peternak sapi perah di Junrejo, Batu menghadapi tantangan dalam meningkatkan produktivitas dan kualitas susu akibat rendahnya kesehatan ternak dan keterbatasan akses terhadap teknologi pakan yang efisien. Program pengabdian masyarakat ini bertujuan untuk meningkatkan pemahaman dan keterampilan peternak dalam memanfaatkan probiotik dan Single Cell Protein (SCP) sebagai pakan tambahan, yang diharapkan dapat meningkatkan kualitas kesehatan dan produktivitas ternak. Program ini menggunakan pendekatan partisipatif yang melibatkan pelatihan langsung dan sesi diskusi untuk memastikan penerapan yang efektif dan berkelanjutan. Probiotik dan SCP berperan dalam memperbaiki mikrobiota usus ternak, meningkatkan efisiensi pencernaan, dan memberikan sumber protein berkualitas tinggi. Hasil pelatihan menunjukkan bahwa setelah mengikuti kegiatan ini, seluruh peserta (100%) memahami dasar-dasar probiotik dan SCP, serta mampu menerapkannya secara mandiri di peternakan mereka. Di samping itu, penggunaan molase sebagai media perbanyakan probiotik mendukung prinsip ekonomi sirkuler, mengurangi limbah produksi gula, dan menciptakan solusi pakan yang ramah lingkungan. Program ini berhasil meningkatkan kapasitas komunitas peternak lokal, sehingga dapat berkontribusi terhadap kesejahteraan ekonomi mereka melalui peningkatan hasil susu dan biaya operasional yang lebih efisien.
Karakterisasi Senyawa Bioaktif, Uji Aktivitas Antimikroba dan Antioksidan Ekstrak Metanol Pepino (Solanum muricatum Aiton) Junairiah, Junairiah; Shabrina, Afrah; Salamun, Salamun; Wanti, Yulia Tri Nurindah; Ulinnuha, Risanda
Jurnal Ilmiah Biosaintropis (Bioscience-Tropic) Vol 10 No 2 (2025): Januari 2025
Publisher : Fakultas Matematika & Ilmu Pengetahuan Alam - Universitas Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/ejbst.v10i2.616

Abstract

Pepino (Solanum muricatum Aiton) merupakan tanaman yang biasa tumbuh pada dataran tinggi dan memiliki berbagai manfaat untuk kesehatan. Penelitian ini bertujuan untuk mengetahui senyawa aktif yang terkandung serta aktivitas antimikroba dan antioksidan dari daun dan buah pepino. Sampel daun dan buah pepino diekstraksi menggunakan pelarut metanol. Ekstrak kental yang didapat diuji dengan uji GC-MS untuk mengetahui senyawa aktif yang ada pada ekstrak. Ekstrak kemudian diuji aktivitas antimikrobanya menggunakan metode difusi cakram pada mikroba patogen Escherichia coli dan Candida albicans, serta aktivitas antioksidan menggunakan metode DPPH. Hasil menunjukkan senyawa aktif terbanyak pada ekstrak metanol daun pepino adalah 2,4-Dimethylbenzo[h]quinoline dengan luas area 23,73% dan ekstrak metanol buah pepino adalah Dianhydroglucitol, TBS 2X dengan luas area 27,19%. Hasil aktivitas antimikroba menunjukkan bahwa ekstrak metanol daun dan buah pepino memiliki aktivitas antimikroba terhadap E. coli dan C. albicans yang dilihat dari zona hambat yang terbentuk. Hasil aktivitas antioksidan ekstrak metanol daun pepino memiliki nilai IC50 sebesar 107,041 µg/mL atau memiliki aktivitas antioksidan sedang dan ekstrak metanol buah pepino memiliki nilai IC50 sebesar 381,904 µg/mL atau memiliki aktivitas antioksidan sangat lemah. Berdasarkan dari banyaknya senyawa yang terkandung pada ekstrak metanol daun dan buah pepino tersebut memiliki aktivitas sebagai antimikroba dan antioksidan.
Co-Authors ., Surahmaida A.A. Ketut Agung Cahyawan W Agus Supriyanto Almando Geraldi Anjar Tri Wibowo Diah Ayu Wulandari Dwi Kusuma Wahyuni Edy Setiti Wida Utami Firli Rahmah Primula Dewi Hamidah . Hery Purnobasuki Hery Suwito Iftitah, Daulah Intan Ayu Pratiwi, Intan Ayu Lilis Sulistyorini Listijani Suhargo, Listijani Manikya Pramudya Muhammad Hilman Fu'adil Amin Nabilah Istighfari Zuraidassanaaz Nabilah Istighfari Zuraidassanaaz Nabilah Istighfari Zuraidassanaaz Ni'matuzahroh Ni'matuzahroh Ni'matuzahroh Ni'matuzahroh Ni'matuzahroh Ni'matuzahroh Ni’matuzahroh Ni’matuzahroh Ni’matuzahroh Ni’matuzahroh Ni’matuzahroh, Ni’matuzahroh Nimatuzahroh, Nimatuzahroh Ni’matuzahroh Ni’matuzahroh Ni’matuzahroh, Ni’matuzahroh Ni’matuzzahroh Ni’matuzahroh Nurul Sofi Amalia Oktavia, Gebby Agnessya Esa Purnomo Purnomo Purnomo Purnomo Rachmah, Artifa Sa'diyah, Muhimmatus Salamun Salamun Shabrina, Afrah Sofiana, Dewi Amelia Sri Puji Astuti Wahyuningsih, Sri Puji Astuti Surahmaida Surahmaida Tri Nurhariyati Tri Puji Lestari Sudarwati Ulinnuha, Risanda Umarudin Umarudin Wanti, Yulia Tri Nurindah Yosephine Sri Wulan Manuhara Zuraidassanaaz, Nabilah Istighfari