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Role of plant growth promoting rhizobacteria on Pteris vittata L as a potential hyperaccumulator plant for gold phytomining agent Aminatun, Tien; Rakhmawati, Anna; Atun, Sri; Idrus, Arifudin; Sujangka, Arsyad
Journal of Degraded and Mining Lands Management Vol. 12 No. 2 (2025)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15243/jdmlm.2025.122.7217

Abstract

Gold phytomining is the extraction of gold from the soil by harvesting specially selected hyperaccumulator plants. One of the potential plant species as a gold hyperaccumulator at the Ratatotok site, North Sulawesi, Indonesia, is Pteris vitata L, possibly because of the presence of rhizobacteria colonies in the roots to help the plant's resistance to metal stress in the soil. The isolation and identification show that the most resistant rhizobacteria to the gold stress is Pseudomonas aeruginosa RTKP1. The study aimed to assess the Pseudomonas aeruginosa RTKP1 to assist the gold bioaccumulation in Pteris vittata L. The phytomining test was carried out with four series of treatments for Pteris vittata L growing media, i.e., (1) tailings without compost and bacterial isolates, (2) tailings with bacterial isolates, (3) tailings with compost, and (4) tailings with compost and bacterial isolate. A descriptive analysis was carried out to analyze the role of the Pseudomnas aeruginosa RTKP1 on gold reduction in the media and gold bioaccumulation in Pteris vittata L. A quantitative analysis was carried out to analyze the bioaccumulation ability of Pteris vittata L with and without the addition of bacterial isolate. The role of the Pseudomonas aeruginosa RTKP1 is to increase the Translocation Factor (TF) and Biological Concentration Factor (BCF), particularly in the roots. The addition of compost to tailings as growing media for Pteris vittata L inhibits the effectiveness of the work of the Pseudomonas aeruginosa RTKP1 in increasing BCF and TF. However, this effect needs to be tested further to obtain significant results.
The Development Electronic Magazine Based on Contextual Teaching And Learning to Increase Logical Thinking Skills and Curiosity of Students on Body Defense System Learning Material Ferella*, Elsa; Rakhmawati, Anna; Efendi, Adhan
Jurnal Pendidikan Sains Indonesia Vol 13, No 1 (2025): JANUARY 2025
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jpsi.v13i1.40832

Abstract

The learning material about body's defence system is considered difficult by students because it related to mechanisms internals organs and abstract concepts. The attempt that able to create is by increasing logical thinking and curiosity. Nevertheless, the lack of learning media used by teachers does not support the ability to think logically and curiosity of students. The purpose of this research is to create learning media based contextual teaching and learning (CTL) through electronic magazines that proper, practical, and effective to improving student's logical thinking and curiosity. The procedure used for the research are ADDIE development model consists of five steps, which are analysis, design, development, implementation, and evaluation. The trial test used a quasi experimental design of nonequivalent control group design. The control and experimental classes were selected using the random sampling method so that there were different numbers of students in each class selected. The trial subjects were divided into control class (35 students) and experimental class (36 students). Based on the results of data analysis revealed that: (1) CTL electronic magazines is proper to use as learning media with a very worthy category; (2) CTL electronic magazines is practical to use as learning media based on learner responses percentage of learning implementation 90% with very good category; (3) CTL electronic magazines are proven effective to increased the logical thinking ability and curiosity of students
Pelatihan Budidaya Maggot untuk Meningkatkan Ketrampilan Masyarakat dalam Pengolahan Limbah Organik Rumah Tangga dan Menciptakan Peluang Usaha di Kelurahan Umbulmartani, Ngemplak, Sleman Octavia, Bernadetta; Rakhmawati, Anna; Aminatun, Tien; Suhartini, Suhartini; Aulia, Fera
Jurnal Pengabdian Masyarakat MIPA dan Pendidikan MIPA Vol. 9 No. 1 (2025): Jurnal Pengabdian Masyarakat MIPA dan Pendidikan MIPA
Publisher : Yogyakarta State University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jpmmp.v9i1.82730

Abstract

The accumulation of rubbish at temporary waste storage locations has become a common concern for us. The organic waste in the rubbish pile usually causes a very strong pungent odor. In fact, if organic waste is managed from every house/location that produces it, it will very significantly reduce both the volume of rubbish piles and the pungent stench. Therefore, it is necessary to instill awareness in every member of society about the importance of managing waste and specifically organic waste which can be degraded by various types of organisms. One of the organisms that can be used to process this organic waste is maggot, which is the larva of the black soldier fly (BSF). Apart from being an organic waste processing agent, maggots can also be used as a feed supplement for chickens and fish. In this way, maggot cultivation can have a dual function for community members who are motivated to manage organic waste independently. Through Lecturer Activities Off Campus (DLK) activities this year, the community in Umbulmartani Village, Ngemplak District, Sleman was given training on Maggot Cultivation to be used as an organic waste processor on a household scale and can also be used to improve the family economy if maggot cultivation is carried out managed seriously. The final activity is evaluation. The program results show the success of the process as indicated by: a. Attendance of participants reaches 100%, b. Increasing knowledge and skills in cultivating maggots for processing family organic waste, c. The results of the participant satisfaction questionnaire showed that participant satisfaction in all aspects was good and very good.
QUALITY OF CRITICAL THINKING, COMMUNICATION, COLLABORATION AND CREATIVITY SKILLS: SURVEY OF HIGH SCHOOL STUDENTS IN BIOLOGY LEARNING Sari, Tasya Novian Indah; Rakhmawati, Anna; Ratnawati, Dwi; Purwanti, Nunuk; Yulianti, Yulianti
Didaktika Biologi: Jurnal Penelitian Pendidikan Biologi Vol. 9 No. 1 (2025): DIDAKTIKA BIOLOGI
Publisher : Universitas Muhammadiyah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32502/didaktikabiologi.v9i1.185

Abstract

Students need critical thinking, creativity, communication and collaboration skills to face and adapt to social change, so that students can survive and compete well in social life. The aim of the research is to describe the critical thinking, collaboration, communication and creativity skills of high school students in biology learning. The research was carried out in August-September 2023. The research population was high school students in Magetan Regency. The research sampling technique was cluster sampling and the research sample was 539 high school class students. Data collection techniques used questionnaires on critical thinking skills, communication, collaboration and creativity. This research instrument is a questionnaire in the form of a questionnaire with 5 Likert scales. Quantitative descriptive research data analysis techniques. The research results show that high school students' critical thinking and creativity skills are in the medium and low categories compared to communication and collaboration skills. The results of the research can be used as a reflection for teachers or future researchers as a follow-up to empower students' critical thinking, communication, collaboration and creativity skills in implementing the Merdeka Curriculum.
Effectiveness of PjBL Worksheet Based on Local Potential to Improve Critical and Creative Thinking Skills of Phase E High School Students Tasya Novian Indah Sari; Anna Rakhmawati; Hanifa Rizky Rahmawati
Journal of Innovation in Educational and Cultural Research Vol 6, No 1 (2025)
Publisher : Yayasan Keluarga Guru Mandiri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46843/jiecr.v6i1.1836

Abstract

Involving local potential as teaching material content is an effort to create more contextual learning and introduce local potential to students. This study aimed to determine the effectiveness of the PjBL-Worksheet based on the regional potential of Gandong River in improving critical and creative thinking skills. The research method is a quasi-experimental nonrandomized control class pretest-posttest design. The study was conducted in January-March 2024. The research population was students of Phase E of SMA 1 Sukomoro, Indonesia. The research sampling technique was cluster random sampling, and the research sample was students of classes Phase E.1 and E.5. The method of collecting data on critical and creative thinking skills was using a test technique. The instrument used to collect data was valid and reliable essay questions. The data were analyzed descriptively and using one-way MANOVA tests. The result of 0.00 0.05 means that applying the PjBL Worksheet based on the local potential of Sungai Gandong was practical in simultaneously improving students' critical and creative thinking skills. The results of further tests showed that the treatment given had a simultaneous effect on critical thinking skills by 68.3% and creative thinking by 62.3%.
Feasibility and Practicality of Project Based Learning Teaching Modules Based on Local Potential to Support the Implementation of the Independent High School Curriculum Novian Indah Sari, Tasya; Rakhmawati, Anna
Jurnal Inovasi Pembelajaran Biologi Vol 6 No 1 (2025)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jipb.v6n1.p1-15

Abstract

The purpose of this study was to determine the feasibility and practicality of the Project Learning Teaching Module based on the local potential of the Gandong River on water pollution material to support the implementation of the Merdeka Curriculum in Senior High Schools. This research method is a quantitative descriptive study. The research was conducted from December 2023 to January 2024. The subjects of the study were 1) Biology learning experts, 2) Material Experts, 3) Biology Teachers, and 4) Grade X High School Students. The data collection method in this study was non-test. Non-test data in the form of expert feasibility assessment data and the practicality of the teaching module. The instruments used in this study were 1) the learning expert feasibility assessment instrument, 2) the material expert feasibility assessment instrument, 3) the teacher practicality assessment instrument, and 4) the student practicality assessment instrument. The research data were analyzed using quantitative description to obtain the percentage of feasibility and practicality of the teaching module. The results of the study showed that the PjBL teaching module based on local river potential on water pollution material for grade X received a feasible and practical assessment by learning experts and biology learning practitioners.
Biodegradation of diisobutyl phthalate using fungi plastisphere isolated from plastic waste in mangrove Kadilangu area Baharudin, Jalu Bahtiar; Rakhmawati, Anna
Jurnal Natural Volume 25 Number 2, June 2025
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Diisobutyl phthalate, an additive in plastic production belonging to the group of xenobiotic substances, has a negative impact on human health, especially on the reproductive system. In Indonesia, the presence of plastisphere fungi is often used as a degrading agent for xenobiotic substances. Plastisphere fungi show enzymatic activity to degrade xenobiotic substances but have not been widely studied. Therefore, this research aims to assess the ability of several genera of plastisphere fungi to degrade diisobutyl phthalate and evaluate their enzyme activity and their tolerance under heavy metal stress. The method for determining degradation used an UV-VIS spectrophotometer at wavelength of 362 nm, fungal identification used profile matching method, the presence of oxidoreductase enzymes in fungi was assessed using qualitative method with selective media (ABTS 200 mg/L, phenol red 50 mg/L, and methylene blue 4%), and their tolerance to heavy metals at a concentration of 50 ppm was analyzed using tolerance index method. The identification results of fungi come from the genera Acremonium and Aspergillus, and tolerance index analysis revealed that these genera have high tolerance to very tolerant (0.91-1.23) to the metals (copper, chromium, and lead) induced stress. Evaluation of oxidoreductase enzyme activity showed that Aspergillus from isolate LT2(1) had laccase, lignin peroxidase, and manganese peroxidase enzymes. Analysis of diisobutyl phthalate degradation using an UV-VIS spectrophotometer with wavelength of 362 nm showed that all isolates were able to degrade diisobutyl phthalate within 14 days of incubation period at room temperature (20oC-23oC) under copper-induced stress, with the highest isolate degrading diisobutyl phthalate is Aspergillus LT2(1) (75.57%), followed by Acremonium PK2(2) (60.65%), and Aspergillus HT3(2) (36.79%). Based on these findings, it can be concluded that Aspergillus LT2(1) shows the best agent to degrade diisobutyl phthalate because it has the ability to regulate the flow of nutrients and energy through the structure of hyphae and mycelial tissues and produces oxidoreductase enzymes that are able to degrade recalcitrant compounds.
Isolation and Identification of Profenofos Pesticide-Tolerant Bacteria from Agriculture Land in Sleman Regency Pertiwi, Melania Citra; Rakhmawati, Anna; Octavia, Bernadetta; Aminatun, Tien; Suhartini
Jurnal Sains Dasar Vol. 14 No. 1 (2025): April 2025
Publisher : Faculty of Mathematics and Natural Science, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The intensive application of organophosphate pesticides containing profenofos as the active compound may lead to soil contamination and the emergence of pesticide-tolerant bacterial strains with potential for bioremediation. Comprehensive identification and characterization of these bacteria are crucial to support sustainable agricultural and environmental management practices. The aim of the study is to analyze the characteristics and identify indigenous bacteria tolerant to profenofos pesticide in Sleman agricultural land, DIY. This study is qualitative research using an exploratory-descriptive. Isolation was carried out by dilution to a level of 10-7 followed by a spread plate on Mineral Salt Medium (MSM) which was given with 100 ppm profenofos pesticide. Bacterial isolates were characterized and then identified using profile matching with reference genera. Based on the results of the study, it is found that 10 bacterial isolates tolerant to organophosphate pesticide with the active ingredient profenofos that have a similarity index of ≥70%. Based on the reference bacteria, 5 different genera are obtained, namely Paracoccus (D3, D8), Micrococcus (D5, D6, D9), Enterococcus (D2, D10), Arthrobacter (D1, D4), and Neisseria (D7). The bacterial isolates obtained have different characteristics. The characteristics of these bacteria are gram positive and negative bacteria with round, irregular, and rhizoid shapes. Their margins are lobate, curled, entire, undulate, and ciliate. Their elevations are umbonate, raised, and flat. The color of the bacteria is white to light orange, with various physiological test results.
Reduction of the Heavy Metal Mercury (Hg) from Paningkaban Gold Mining Tailings, Bayumas, with Ageratum Conyzoides Althaaf, Nabila Tsabita; Aminatun, Tien; Rakhmawati, Anna; Atun, Sri
Jurnal Sains Dasar Vol. 14 No. 1 (2025): April 2025
Publisher : Faculty of Mathematics and Natural Science, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Gold is a fairly large contributor to Indonesia's foreign exchange. The area that has the potential to have minerals is Banyumas Regency. This area has been indicated to have gold mineral reserves of around 16,000 hectares. Until now, the gold processing process still uses mercury, which produces negative impacts such as post-mining waste containing heavy metal mercury (Hg), which can cause environmental damage. Environmental damage that arises, such as pollution of the soil and water environment, due to the gold bioleaching process that is disposed of without any treatment. One alternative that can be used to overcome this problem is by using hyperaccumulator plants. One plant that falls into the hyperaccumulator category is the bandotan plant (Ageratum conyzoides), because it is often found in gold mining areas contaminated with mercury. Mercury contamination will be absorbed from the environment into the body of Ageratum conyzoides through membranes and converted into less toxic substances. The aim of this research is to determine the ability of the Ageratum conyzoides plant as a phytoremediation agent in reducing mercury metal (Hg) in tailings from Paningkaban Village, Banyumas. There are two treatments, namely the planting media used with 2 kg of tailings soil and 2 kg of planting media mixed with tailings and compost. The mixture ratio between tailings and compost is 1:1. Each treatment, consisting of three replications, was observed using plant growth parameters (plant height and number of leaves), as well as control parameters (edaphic and microclimatic). The results of the research show that the Ageratum conyzoides plant is a phytoremediation agent that can reduce levels of the heavy metal mercury (Hg) in the planting medium of mining tailings soil from Paningkaban Village, because it gives test results of BCF values > 1 and TF < 1, which indicates that the Ageratum conyzoides plant has potential as a phytostabilizing agent.
Integrating Neem’s Medicinal Properties into Microbiology Education A Systematic Review Muhammad Anees; Anna Rakhmawati; Kartika Ratna Pertiwi; Sajid Iqbal
Quagga: Jurnal Pendidikan dan Biologi Vol 17 No 2 (2025): QUAGGA : Jurnal Pendidikan dan Biologi
Publisher : Program Studi Pendidikan Biologi, Fakultas Keguruan dan Ilmu Pendidikan, Universitas Kuningan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25134/quagga.v17i2.385

Abstract

This systematic review investigates the integration of Azadirachta indica (Neem) as potential learning resources into Microbiology topics in Biology learning, focusing on its bioactive compounds and medicinal properties such as antimicrobial agent. Neem’s bioactive compounds, such as azadirachtin, nimbolide, and gedunin, exhibit potent antimicrobial activity against a broad spectrum of pathogens, including E. coli, S. aureus, and Candida albicans. These properties make Neem a compelling natural alternative in the fight against antimicrobial resistance (AMR), a growing global health concern. Drawing on 20 studies published between 2014 and 2024, this review highlights Neem’s values in microbiology curricula by offering students hands-on opportunities to study natural antimicrobial agents while fostering critical thinking and practical skills in microbiology. Additionally, incorporating Neem into education promotes environmental sustainability whilst acts as an appreciation for traditional local wisdom