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All Journal VALENSI Jurnal Penelitian Saintek Jurnal Ilmu Lingkungan Jurnal Penelitian dan Pengembangan Pendidikan Jurnal Sains Dasar Journal of Degraded and Mining Lands Management Jurnal Penelitian Pendidikan IPA (JPPIPA) ALCHEMY Jurnal Penelitian Kimia Jurnal Pengabdian Masyarakat MIPA dan Pendidikan MIPA Molekul: Jurnal Ilmiah Kimia Journal of Innovation in Educational and Cultural Research JIPB (Jurnal Inovasi Pembelajaran Biologi) Proceeding Biology Education Conference Kaunia: Integration and Interconnection of Islam and Science Journal Research and Development in Education (RaDEn) Jurnal Natural Jurnal Pendidikan Sains Indonesia (Indonesian Journal of Science Education) Jurnal Edukasi Biologi Kingdom (The Journal of Biological Studies) Quagga: Jurnal Pendidikan dan Biologi IJOBI Didaktika Biologi: Jurnal Penelitian Pendidikan Biologi
Anna Rakhmawati
Biology Education Department, Faculty Of Mathematics And Natural Science, Yogyakarta State University, Indonesia
Religion Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Energy Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Physics Public Health Social Sciences Other

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ISOLASI DAN IDENTIFIKASI BAKTERI TANAH SEKITAR LIMBAH MASKER MEDIS DARI MANGROVE WANA TIRTA, KULON PROGO, YOGYAKARTA Ratnasari, Tantri Ayu; Rakhmawati, Anna
Jurnal Sains Dasar Vol 13, No 2 (2024): Oktober 2024
Publisher : Faculty of Mathematics and Natural Science, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jsd.v13i2.70229

Abstract

Tujuan penelitian ini adalah untuk mengetahui karakteristik dan genus bakteri yang mampu hidup menggunakan masker medis sebagai sumber karbonnya. Penelitian ini merupakan penelitian kualitatif dengan jenis penelitian eksploratif-deskriptif yang dilakukan dengan mengeksplorisasi bakteri tanah mangrove dari sekitar limbah masker medis yang ada di kawasan ekowisata Mangrove Wana Tirta, Dusun Pasir Mendit, Desa Jangkaran, Kecamatan Temon, Kabupaten Kulon Progo, Provinsi Daerah Istimewa Yogyakarta. Isolasi dilakukan dengan menggunakan media Luria Bertani (LB) yang mengandung banyak nutrisi yang diperlukan oleh bakteri. Isolat kemudian diseleksi menggunakan Minimum Salt Media (MSM) dan ditambahkan potongan masker medis biru sebagai satu-satunya sumber karbon serta dicek nilai OD-nya. Isolat hasil seleksi selanjutnya dikarakterisasi untuk didapatkan ciri-ciri fenotipenya berupa pengamatan mikroskopis, makroskopis, uji biokimia, dan uji fisiologis. Hasil karakter digunakan untuk profil matching dengan genus bakteri acuan dan didapatkan dendogram yang menunjukkan hubungan kekerabatan dengan nilai similaritas tertentu. Berdasarkan hasil penelitian, didapatkan 10 isolat yang menunjukkan kenaikan nilai OD. Isolat tersebut diduga memiliki hubungan kekerabatan dengan 8 genus bakteri dengan nilai similaritas 70%, yakni Arthrobacter (isolat 10), Azotobacter (isolat 21), Chelatococcus (isolat 23), Enterococcus (isolat 9 dan 17), Neisseria (isolat 26), Paracoccus (isolat 11), Pseudomonas (isolat 20), dan Serratia (isolat 2 dan 29).
Website-Based Learning Media in High School Biology Learning on Genetic Substance Material Ega Safitri; Anna Rakhmawati
Jurnal Penelitian dan Pengembangan Pendidikan Vol. 8 No. 3 (2024): October
Publisher : LPPM Undiksha

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23887/jppp.v8i3.79445

Abstract

Genetic substance material requires learning media in its delivery because it cannot be seen directly, making it difficult for students to understand. This study aims to create a website-based learning media suitable for high school biology learning on genetic substance material. The research method used is research and development (R&D) with the ADDIE model. The study subjects included material and media experts, teachers, and 13 grade XII high school students. Data collection was carried out using interview, observation, and questionnaire methods. Data analysis techniques used qualitative and quantitative analysis. The results of the study showed that the validation of material experts obtained a score of 100% (very feasible), media experts obtained a score of 87.50% (very possible), individual trials obtained a score of 100% (very possible), and limited trials obtained a score of 85.79% (very feasible). It was concluded that website-based learning media is suitable for application in biology learning on genetic substance material. The implications of this study are to motivate teachers to use media in the learning process at school.
Role of plant growth promoting rhizobacteria on Pteris vittata L as a potential hyperaccumulator plant for gold phytomining agent Aminatun, Tien; Rakhmawati, Anna; Atun, Sri; Idrus, Arifudin; Sujangka, Arsyad
Journal of Degraded and Mining Lands Management Vol. 12 No. 2 (2025)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15243/jdmlm.2025.122.7217

Abstract

Gold phytomining is the extraction of gold from the soil by harvesting specially selected hyperaccumulator plants. One of the potential plant species as a gold hyperaccumulator at the Ratatotok site, North Sulawesi, Indonesia, is Pteris vitata L, possibly because of the presence of rhizobacteria colonies in the roots to help the plant's resistance to metal stress in the soil. The isolation and identification show that the most resistant rhizobacteria to the gold stress is Pseudomonas aeruginosa RTKP1. The study aimed to assess the Pseudomonas aeruginosa RTKP1 to assist the gold bioaccumulation in Pteris vittata L. The phytomining test was carried out with four series of treatments for Pteris vittata L growing media, i.e., (1) tailings without compost and bacterial isolates, (2) tailings with bacterial isolates, (3) tailings with compost, and (4) tailings with compost and bacterial isolate. A descriptive analysis was carried out to analyze the role of the Pseudomnas aeruginosa RTKP1 on gold reduction in the media and gold bioaccumulation in Pteris vittata L. A quantitative analysis was carried out to analyze the bioaccumulation ability of Pteris vittata L with and without the addition of bacterial isolate. The role of the Pseudomonas aeruginosa RTKP1 is to increase the Translocation Factor (TF) and Biological Concentration Factor (BCF), particularly in the roots. The addition of compost to tailings as growing media for Pteris vittata L inhibits the effectiveness of the work of the Pseudomonas aeruginosa RTKP1 in increasing BCF and TF. However, this effect needs to be tested further to obtain significant results.
The Development Electronic Magazine Based on Contextual Teaching And Learning to Increase Logical Thinking Skills and Curiosity of Students on Body Defense System Learning Material Ferella*, Elsa; Rakhmawati, Anna; Efendi, Adhan
Jurnal Pendidikan Sains Indonesia Vol 13, No 1 (2025): JANUARY 2025
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jpsi.v13i1.40832

Abstract

The learning material about body's defence system is considered difficult by students because it related to mechanisms internals organs and abstract concepts. The attempt that able to create is by increasing logical thinking and curiosity. Nevertheless, the lack of learning media used by teachers does not support the ability to think logically and curiosity of students. The purpose of this research is to create learning media based contextual teaching and learning (CTL) through electronic magazines that proper, practical, and effective to improving student's logical thinking and curiosity. The procedure used for the research are ADDIE development model consists of five steps, which are analysis, design, development, implementation, and evaluation. The trial test used a quasi experimental design of nonequivalent control group design. The control and experimental classes were selected using the random sampling method so that there were different numbers of students in each class selected. The trial subjects were divided into control class (35 students) and experimental class (36 students). Based on the results of data analysis revealed that: (1) CTL electronic magazines is proper to use as learning media with a very worthy category; (2) CTL electronic magazines is practical to use as learning media based on learner responses percentage of learning implementation 90% with very good category; (3) CTL electronic magazines are proven effective to increased the logical thinking ability and curiosity of students
Pelatihan Budidaya Maggot untuk Meningkatkan Ketrampilan Masyarakat dalam Pengolahan Limbah Organik Rumah Tangga dan Menciptakan Peluang Usaha di Kelurahan Umbulmartani, Ngemplak, Sleman Octavia, Bernadetta; Rakhmawati, Anna; Aminatun, Tien; Suhartini, Suhartini; Aulia, Fera
Jurnal Pengabdian Masyarakat MIPA dan Pendidikan MIPA Vol. 9 No. 1 (2025): Jurnal Pengabdian Masyarakat MIPA dan Pendidikan MIPA
Publisher : Yogyakarta State University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jpmmp.v9i1.82730

Abstract

The accumulation of rubbish at temporary waste storage locations has become a common concern for us. The organic waste in the rubbish pile usually causes a very strong pungent odor. In fact, if organic waste is managed from every house/location that produces it, it will very significantly reduce both the volume of rubbish piles and the pungent stench. Therefore, it is necessary to instill awareness in every member of society about the importance of managing waste and specifically organic waste which can be degraded by various types of organisms. One of the organisms that can be used to process this organic waste is maggot, which is the larva of the black soldier fly (BSF). Apart from being an organic waste processing agent, maggots can also be used as a feed supplement for chickens and fish. In this way, maggot cultivation can have a dual function for community members who are motivated to manage organic waste independently. Through Lecturer Activities Off Campus (DLK) activities this year, the community in Umbulmartani Village, Ngemplak District, Sleman was given training on Maggot Cultivation to be used as an organic waste processor on a household scale and can also be used to improve the family economy if maggot cultivation is carried out managed seriously. The final activity is evaluation. The program results show the success of the process as indicated by: a. Attendance of participants reaches 100%, b. Increasing knowledge and skills in cultivating maggots for processing family organic waste, c. The results of the participant satisfaction questionnaire showed that participant satisfaction in all aspects was good and very good.
QUALITY OF CRITICAL THINKING, COMMUNICATION, COLLABORATION AND CREATIVITY SKILLS: SURVEY OF HIGH SCHOOL STUDENTS IN BIOLOGY LEARNING Sari, Tasya Novian Indah; Rakhmawati, Anna; Ratnawati, Dwi; Purwanti, Nunuk; Yulianti, Yulianti
Didaktika Biologi: Jurnal Penelitian Pendidikan Biologi Vol. 9 No. 1 (2025): DIDAKTIKA BIOLOGI
Publisher : Universitas Muhammadiyah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32502/didaktikabiologi.v9i1.185

Abstract

Students need critical thinking, creativity, communication and collaboration skills to face and adapt to social change, so that students can survive and compete well in social life. The aim of the research is to describe the critical thinking, collaboration, communication and creativity skills of high school students in biology learning. The research was carried out in August-September 2023. The research population was high school students in Magetan Regency. The research sampling technique was cluster sampling and the research sample was 539 high school class students. Data collection techniques used questionnaires on critical thinking skills, communication, collaboration and creativity. This research instrument is a questionnaire in the form of a questionnaire with 5 Likert scales. Quantitative descriptive research data analysis techniques. The research results show that high school students' critical thinking and creativity skills are in the medium and low categories compared to communication and collaboration skills. The results of the research can be used as a reflection for teachers or future researchers as a follow-up to empower students' critical thinking, communication, collaboration and creativity skills in implementing the Merdeka Curriculum.
Effectiveness of PjBL Worksheet Based on Local Potential to Improve Critical and Creative Thinking Skills of Phase E High School Students Tasya Novian Indah Sari; Anna Rakhmawati; Hanifa Rizky Rahmawati
Journal of Innovation in Educational and Cultural Research Vol 6, No 1 (2025)
Publisher : Yayasan Keluarga Guru Mandiri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46843/jiecr.v6i1.1836

Abstract

Involving local potential as teaching material content is an effort to create more contextual learning and introduce local potential to students. This study aimed to determine the effectiveness of the PjBL-Worksheet based on the regional potential of Gandong River in improving critical and creative thinking skills. The research method is a quasi-experimental nonrandomized control class pretest-posttest design. The study was conducted in January-March 2024. The research population was students of Phase E of SMA 1 Sukomoro, Indonesia. The research sampling technique was cluster random sampling, and the research sample was students of classes Phase E.1 and E.5. The method of collecting data on critical and creative thinking skills was using a test technique. The instrument used to collect data was valid and reliable essay questions. The data were analyzed descriptively and using one-way MANOVA tests. The result of 0.00 0.05 means that applying the PjBL Worksheet based on the local potential of Sungai Gandong was practical in simultaneously improving students' critical and creative thinking skills. The results of further tests showed that the treatment given had a simultaneous effect on critical thinking skills by 68.3% and creative thinking by 62.3%.
Feasibility and Practicality of Project Based Learning Teaching Modules Based on Local Potential to Support the Implementation of the Independent High School Curriculum Novian Indah Sari, Tasya; Rakhmawati, Anna
Jurnal Inovasi Pembelajaran Biologi Vol 6 No 1 (2025)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jipb.v6n1.p1-15

Abstract

The purpose of this study was to determine the feasibility and practicality of the Project Learning Teaching Module based on the local potential of the Gandong River on water pollution material to support the implementation of the Merdeka Curriculum in Senior High Schools. This research method is a quantitative descriptive study. The research was conducted from December 2023 to January 2024. The subjects of the study were 1) Biology learning experts, 2) Material Experts, 3) Biology Teachers, and 4) Grade X High School Students. The data collection method in this study was non-test. Non-test data in the form of expert feasibility assessment data and the practicality of the teaching module. The instruments used in this study were 1) the learning expert feasibility assessment instrument, 2) the material expert feasibility assessment instrument, 3) the teacher practicality assessment instrument, and 4) the student practicality assessment instrument. The research data were analyzed using quantitative description to obtain the percentage of feasibility and practicality of the teaching module. The results of the study showed that the PjBL teaching module based on local river potential on water pollution material for grade X received a feasible and practical assessment by learning experts and biology learning practitioners.
Biodegradation of diisobutyl phthalate using fungi plastisphere isolated from plastic waste in mangrove Kadilangu area Baharudin, Jalu Bahtiar; Rakhmawati, Anna
Jurnal Natural Volume 25 Number 2, June 2025
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Diisobutyl phthalate, an additive in plastic production belonging to the group of xenobiotic substances, has a negative impact on human health, especially on the reproductive system. In Indonesia, the presence of plastisphere fungi is often used as a degrading agent for xenobiotic substances. Plastisphere fungi show enzymatic activity to degrade xenobiotic substances but have not been widely studied. Therefore, this research aims to assess the ability of several genera of plastisphere fungi to degrade diisobutyl phthalate and evaluate their enzyme activity and their tolerance under heavy metal stress. The method for determining degradation used an UV-VIS spectrophotometer at wavelength of 362 nm, fungal identification used profile matching method, the presence of oxidoreductase enzymes in fungi was assessed using qualitative method with selective media (ABTS 200 mg/L, phenol red 50 mg/L, and methylene blue 4%), and their tolerance to heavy metals at a concentration of 50 ppm was analyzed using tolerance index method. The identification results of fungi come from the genera Acremonium and Aspergillus, and tolerance index analysis revealed that these genera have high tolerance to very tolerant (0.91-1.23) to the metals (copper, chromium, and lead) induced stress. Evaluation of oxidoreductase enzyme activity showed that Aspergillus from isolate LT2(1) had laccase, lignin peroxidase, and manganese peroxidase enzymes. Analysis of diisobutyl phthalate degradation using an UV-VIS spectrophotometer with wavelength of 362 nm showed that all isolates were able to degrade diisobutyl phthalate within 14 days of incubation period at room temperature (20oC-23oC) under copper-induced stress, with the highest isolate degrading diisobutyl phthalate is Aspergillus LT2(1) (75.57%), followed by Acremonium PK2(2) (60.65%), and Aspergillus HT3(2) (36.79%). Based on these findings, it can be concluded that Aspergillus LT2(1) shows the best agent to degrade diisobutyl phthalate because it has the ability to regulate the flow of nutrients and energy through the structure of hyphae and mycelial tissues and produces oxidoreductase enzymes that are able to degrade recalcitrant compounds.
Isolation and Identification of Profenofos Pesticide-Tolerant Bacteria from Agriculture Land in Sleman Regency Pertiwi, Melania Citra; Rakhmawati, Anna; Octavia, Bernadetta; Aminatun, Tien; Suhartini
Jurnal Sains Dasar Vol. 14 No. 1 (2025): April 2025
Publisher : Faculty of Mathematics and Natural Science, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The intensive application of organophosphate pesticides containing profenofos as the active compound may lead to soil contamination and the emergence of pesticide-tolerant bacterial strains with potential for bioremediation. Comprehensive identification and characterization of these bacteria are crucial to support sustainable agricultural and environmental management practices. The aim of the study is to analyze the characteristics and identify indigenous bacteria tolerant to profenofos pesticide in Sleman agricultural land, DIY. This study is qualitative research using an exploratory-descriptive. Isolation was carried out by dilution to a level of 10-7 followed by a spread plate on Mineral Salt Medium (MSM) which was given with 100 ppm profenofos pesticide. Bacterial isolates were characterized and then identified using profile matching with reference genera. Based on the results of the study, it is found that 10 bacterial isolates tolerant to organophosphate pesticide with the active ingredient profenofos that have a similarity index of ≥70%. Based on the reference bacteria, 5 different genera are obtained, namely Paracoccus (D3, D8), Micrococcus (D5, D6, D9), Enterococcus (D2, D10), Arthrobacter (D1, D4), and Neisseria (D7). The bacterial isolates obtained have different characteristics. The characteristics of these bacteria are gram positive and negative bacteria with round, irregular, and rhizoid shapes. Their margins are lobate, curled, entire, undulate, and ciliate. Their elevations are umbonate, raised, and flat. The color of the bacteria is white to light orange, with various physiological test results.
Co-Authors Abdullah Nashih Amin Ajeng Septiana Putri Aji Suhandy Althaaf, Nabila Tsabita Aminatun, Tien Arifudin Idrus Astuti Astuti Aulia, Fera Ayu Natasya FR BAHARUDIN, JALU BAHTIAR Baso Samsu Rijal Bernadetta Octavia Bidhari Pidhatika Budiwati Budiwati David Meilana Nur Arifin Dhiya Millatika Tohiri Drajat Pramiadi Dwi Ratnawati, Dwi Efendi, Adhan Ega Safitri Eli Rohaeti Endang W Laksono Endang Widjajanti Laksono FX Endang WLFX Eny Purwanti Evi Linda Evy Yulianti Ferella*, Elsa Fitri Rahmawati Hafizh Adyatma Hajidah Musyayyadah Hanifa Rizky Rahmawati Intan Lestari Isroni, Muhson Istiana Prihatini Kartika Ratna Pertiwi Kun Sri Budiasih Kurnia Cahyani Lili Sugiyarto Marfuatun . Muhammad Anees Novian Indah Sari, Tasya Nrangwesthi Widyaningrum Nunuk Purwanti Nur Fathurahman Ridwan Nur Rizki Putri Ramadhanti Paidi Paskariani, Angela Enggar Pertiwi, Melania Citra Pramesty, Alfinda Rania Nur Farida Ratnasari, Tantri Ayu Ria Dewi Dili Hastuti Ria Dewi Dili Hastuti Rindiana, Danny Ria Rizka Apriani Putri Sajid Iqbal Sari, Tasya Novian Indah Setiawan, Fauzi Fandy Simbolon, Doly Siti Umniyati Siti Umniyatie Siti Umniyatie Siti Umniyatie SRI ATUN Suhartini Suhartini Suhartini Suhartini Suhartini Sujangka, Arsyad Tasya Novian Indah Sari Tutiek Rahayu Wahyu Nuryadi H Yulianti Yulianti Yuliati Yuliati Yuni Wibowo