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All Journal Jurnal Penelitian Saintek Jurnal Ilmu Lingkungan Jurnal Penelitian dan Pengembangan Pendidikan Jurnal Sains Dasar Journal of Degraded and Mining Lands Management Jurnal Penelitian Pendidikan IPA (JPPIPA) ALCHEMY Jurnal Penelitian Kimia Jurnal Pengabdian Masyarakat MIPA dan Pendidikan MIPA Molekul: Jurnal Ilmiah Kimia Journal of Innovation in Educational and Cultural Research JIPB (Jurnal Inovasi Pembelajaran Biologi) Proceeding Biology Education Conference Kaunia: Integration and Interconnection of Islam and Science Journal Research and Development in Education (RaDEn) Jurnal Natural Jurnal Pendidikan Sains Indonesia (Indonesian Journal of Science Education) Jurnal Edukasi Biologi Kingdom (The Journal of Biological Studies) Quagga: Jurnal Pendidikan dan Biologi Didaktika Biologi: Jurnal Penelitian Pendidikan Biologi
Anna Rakhmawati
Universitas Negeri Yogyakarta
Religion Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Energy Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Physics Public Health Social Sciences Other

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Assessing soil bacterial community response to organophosphate pesticides in agricultural field of Yogyakarta, Java, Indonesia ANNA RAKHMAWATI; BERNADETTA OCTAVIA; SUHARTINI SUHARTINI; TIEN AMINATUN
Jurnal Natural Volume 23 Number 3, October 2023
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v23i3.31263

Abstract

Soil contamination by pesticides is one of the world’s most pressing environmental issues. The widespread use of Organophosphate pesticides (OPPs) in agriculture has led to biological diversity changes. The indigenous bacterial community played significant roles in the remediation of soil contaminated with OPPs. This study examines the overall bacterial community composition of three agricultural fields in Yogyakarta, Java, Indonesia, that were exposed to OPPs. The agricultural field was divided into zones near the beach, residential, and mountainous. Sequencing 16S rRNA amplicon fragments used to analyze the soil bacterial community. It was discovered that Proteobacteria, Actinobacteria, and Firmicutes comprised the majority of the bacterial community. In addition, the samples contain a high relative abundance of Bacillus, Bradyrhizobium, Chryseobacterium, Cystobacter, Microvirga, and Burkholderia. The high alpha diversity indexes suggest that the agricultural soil microbiome provides important ecological services and may harbor a wide variety of bacteria and genes with biotechnological applications. The physicochemical soil characteristics are also correlated with the bacterial community structure. The findings can be used to develop bioremediation strategies that employ native microbes to clean and restore agricultural soil contaminated with OPPs.
Identifikasi Logam Berat Kromium di Tiga Sungai yang Melintasi Kota Yogyakarta dan Potensi Fitoremediasinya Tien Aminatun; Anna Rakhmawati; Kun Sri Budiasih; Marfuatun Marfuatun; Baso Samsu Rijal; Abdullah Nashih Amin; David Meilana Nur Arifin; Ajeng Septiana Putri
Jurnal Ilmu Lingkungan Vol 22, No 3 (2024): May 2024
Publisher : School of Postgraduate Studies, Diponegoro Univer

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jil.22.3.620-631

Abstract

Winongo, Gajah Wong, dan Code adalah tiga sungai yang melintasi Yogyakarta. Banyak industri yang membuang limbah ke ketiga sungai ini, sedangkan Cr adalah logam berat yang banyak terdapat pada limbah industri. Berbagai penelitian telah dilakukan untuk melihat potensi fitoremediasi Cr, tetapi umumnya pada limbah cair industri, bukan pada perairan langsung. Tujuan penelitian ini adalah mengidentifikasi logam berat Kromium (Cr) di ketiga sungai serta potensi fitoremediasinya. Sampel air dan sedimen diambil dari hulu, tengah dan hilir, kemudian kandungan Cr dianalisis dengan Spektrofotometri UV-Vis di Laboratorium Kimia Fakultas MIPA Universitas Negeri Yogyakarta (UNY). Untuk melihat potensi fitoremediasinya dilakukan eksperimen dengan eceng gondok (Eichornia crassipes) dan kayu apu (Pistia stratiotes) di greenhouse FMIPA UNY. Pengukuran kualitas air diujikan ke Laboratorium BBTKL-PP. Hasil penelitian menunjukkan ketiga sungai teridentifikasi cemaran Cr tetapi masih di bawah ambang baku mutu menurut Peraturan Gubernur DIY Nomor 20 Tahun 2008 tentang Baku Mutu Air di Provinsi DIY Kelas 1. Potensi eceng gondok dan kayu apu sebagai fitoremediator air sungai yang tercemar Cr tidak tampak jelas karena konsentrasi Cr pada ketiga sungai masih rendah di bawah ambang baku mutu (<0.05 mg/L), akan tetapi dilihat dari performa tanaman maka kedua gulma ini berpotensi untuk menjadi fitoremediator di sungai yang tercemar Cr.
Analysis the quality of critical thinking and creativity questions in high school biology subjects with the Rasch model Tasya Novian Indah Sari; Anna Rakhmawati
Research and Development in Education (RaDEn) Vol. 4 No. 1 (2024): July
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/raden.v4i1.32758

Abstract

A good instrument is an instrument that can measure students' abilities accurately. The research aims to analyze the quality of critical thinking and creativity items on water pollution material in terms of validity, reliability, and level of difficulty using the Rasch model. The research used a quantitative descriptive method involving biology learning experts and 173 class X high school students. The research was carried out in December 2023-January 2024. The data collection method used expert assessment sheets and critical thinking and creativity essay questions. Data were analyzed using the Rasch model assisted by Quest. The results showed that of the 10 critical thinking essay questions, 3 questions were invalid, while of the 8 creativity essay questions, 1 question was invalid based on the suitability of the INFIT MNSQ and OUTFIT T scores. The results of the reliability test with Cronbach’s alpha showed that the reliability value of the critical thinking instrument was 0.74 and creativity 0.79. Average difficulty level of questions with threshold values in the medium and difficult categories. Overall, the instrument was feasible and question items that did not fit the Rasch model were removed.
IDENTIFIKASI MATERI SULIT KOMPETENSI DASAR BAKTERI PADA SISWA KELAS X SEMESTER I DI SMA NEGERI 1 KOTA MUNGKID Rindiana, Danny Ria; Rakhmawati, Anna
Jurnal Edukasi Biologi Vol 8, No 2 (2022): Jurnal Edukasi Biologi
Publisher : Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/edubio.v8i2.18386

Abstract

Penelitian ini bertujuan untuk mengetahui letak materi sulit kompetensi dasar bakteri pada siswa kelas X Semester I di SMA Negeri 1 Kota Mungkid Tahun Ajaran 2021/2022 berdasarkan submateri. Penelitian ini merupakan penelitian deskriptif. Sampel pada penelitian ini ditentukan dengan teknik cluster sampling. Instrumen penelitian berupa soal tes kompetensi dasar bakteri. Analisis data dilakukan dengan menghitung ketercapaian belajar siswa kemudian dibandingkan dengan kriteria tingkat kesulitan materi menurut teori. Hasil Penelitian menunjukkan letak materi sulit kompetensi dasar bakteri berdasarkan submateri adalah pada submateri karakteristik bakteri, klasifikasi bakteri dan reproduksi bakteri dengan tingkat kesulitan sukar. Sub materi peran bakteri dan definisi bakteri memiliki tingkat kesulitan sedang. Urutan materi sulit dari tinggi ke rendah adalah submateri karakteristik bakteri, klasifikasi bakteri, reproduksi bakteri, peran bakteri dan definisi bakteri.
ISOLASI, KARAKTERISASI, DAN IDENTIFIKASI BAKTERI HALOFILIK PENGHASIL PROTEASE DARI IKAN ASIN LAYUR (Trichiurus lepturus) DI PASAR BERINGHARJO YOGYAKARTA Pramesty, Alfinda; Rakhmawati, Anna
Kingdom: The Journal of Biological Studies Vol 9, No 2 (2023): Kingdom: The Journal of Biological Studies
Publisher : Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/kingdom.v9i2.18363

Abstract

Pembuatan ikan asin layur (Trichiurus lepturus) menggunakan ikan air laut dari genus Trichiurus yang berpotensi mengandung bakteri halolifik. Ikan asin memiliki potensi besar sebagai sumber biomassa penghasil enzim protease yang tahan terhadap kadar garam tinggi (halostabil). Tujuan dari penelitian ini untuk mengetahui untuk mengetahui karakteristik dan genus, serta hubungan kekerabatan antar isolat bakteri halofilik penghasil protease dari ikan asin layur (T. Lepturus) di Pasar Beringharjo Yogyakarta. Penelitian ini merupakan penelitian deskriptif eksploratif dengan metode observasi. Pengambilan sampel ikan asin layur (T. lepturus) dilakukan pada 3 kios pedagang berbeda berdasarkan daerah hasil produksi pengolahan ikan asin tersebut. Sampel selanjutnya diisolasi pada media selektif Skim Milk Agar + 3% NaCl untuk menumbuhkan bakteri halofilik penghasil protease. Isolat bakteri yang didapatkan kemudian dimurnikan dan dilukan karakterisasi fenetik meliputi morfologi koloni dan sel, fisiologis (biokimia), serta kemampuan tumbuh pada kadar NaCl 0%, 10%, dan 20%. Data yang diperoleh digunakan untuk identifikasi dengan metode matching profile berdasarkan Bergey’s Manual of Determinative Bacteriology. Hubungan kekerabatan antar isolat bakteri diklasifikasikan menggunakan software MVSP 3.1 dengan algoritma pengklasteran UPGMA dan dikonstruksikan dalam bentuk dendogram. Hasil penelitian menunjukkan terdapat 7 isolat bakteri halofilik penghasil protease dari ikan asin layur (T. lepturus) yang berhasil diisolasi dari kios pedagang berbeda yaitu 3 isolat berasal dari kios A, 3 isolat berasal dari kios B, dan 1 isolat berasal dari kios C. konstruksi dendogram menunjukan 9 klaster bakteri. Terdapat 2 isolat bakteri yang memiliki indeks similaritas ≥ 83% terhadap acuan Staphylococcus sp. yaitu isolat A5 dan C6. Sebanyak 4 isolat bakteri yang memiliki indeks similaritas ≥ 67% terhadap Salinococcus sp. yaitu A6, A8, B5, dan B6. Serta 1 isolat bakteri yang memiliki indeks similaritas 83% terhadap strain acuan Halomonas sp. yaitu isolat B6.Kata kunci : Isolasi;Bakteri Halofilik;Proteolitik;Karakter FenetikIsolasi, Bakteri Halofilik, Proteolitik, Karakter Fenetik.
Lithogeochemical characteristics and potential hyperaccumulator identification as phytomining agent at the Ratatotok gold mine, Indonesia Aminatun, Tien; Idrus, Arifudin; Simbolon, Doly; Rakhmawati, Anna; Atun, Sri
Journal of Degraded and Mining Lands Management Vol. 11 No. 2 (2024)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15243/jdmlm.2024.112.5251

Abstract

In the future, phytomining will be an environmentally friendly alternative mining technology. Therefore, the exploration of vegetation types having the potential as hyperaccumulators in gold phytomining needs to be carried out. This study aimed to (1) investigate the effect of rock/soil mineralogy characteristics and Au content on the diversity of vegetation types at gold mine sites, and (2) determine the type of potential hyperaccumulator vegetation as a phytomining agent based on the Biological Concentration Factor (BCF). This study was conducted at the Ratatotok gold mine in North Sulawesi Province, Indonesia. The sampling locations consisted of 3 sites, i.e., Bulex, Yance, and Leon, and each site consisted of 5 sampling plots. Soil samples were taken from each sampling plot and then tested for gold content using the ICP-MS method and mineral content using the XRD method. Mineralized bedrock samples were also taken for mineralogical analysis through petrography. Data analysis of soil geochemistry was carried out descriptively. Quantitative descriptive analysis was also carried out to determine the dominant type of vegetation, which was potential for hyperaccumulators at the mine site. The results showed that gold content in the soil affected the diversity of vegetation, which was possibly due to gold stress, which affected plant growth. Based on the BCF value, three local plant species having potential as gold hyperaccumulators with moderate bioaccumulation ability (BCF>0.1-1) were found, namely Pteris vittata, Syzygium aromaticum, and Swietenia mahagoni. However, the use of these plants as phytomining agents requires further research.
Development of PjBL-based e-worksheets utilizing the kombucha bioprocess in high school biology learning Dhiya Millatika Tohiri; Anna Rakhmawati
Research and Development in Education (RaDEn) Vol. 4 No. 2 (2024): December
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/raden.v4i2.36263

Abstract

Worksheets are still often made in the format of questions that are done individually. Kombucha, when viewed from the characteristics of the manufacturing process, have the potential to be innovations in conventional biology technology innovation learning. This study aimed to assess the feasibility and practicality of using project-based learning electronic worksheets with kombucha bioprocess for teaching biology. The research method employed was development research using the ADDIE model, focusing on the analysis, design, and development stages. The study involved one material expert, one media expert, two biology teachers, and 33 students. Data was collected using interviews and questionnaires. The electronic worksheet was evaluated by media and material expert lecturers for feasibility and by teachers and students for practicality The results indicated that this worksheet is both feasible and practical for biology learning activities with a material validity level of 72.6% (feasible), media validity of 98% (very feasible), biology teacher response 92% (very practical) and student response 85% (very practical). The findings of this study are expected to be an inspiration for educators in compiling media for learning activities, becoming the latest and most interesting learning content innovations in project creation.
PENGEMBANGAN LKPD BERBASIS PROJECT BASED LEARNING UNTUK MENINGKATKAN KETERAMPILAN KOLABORASI SISWA MATERI BIOTEKNOLOGI Lestari, Intan; Rakhmawati, Anna
Jurnal Edukasi Biologi Vol 10, No 2 (2024): Jurnal Edukasi Biologi
Publisher : Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/edubio.v10i2.19644

Abstract

Abstrak. Tujuan penelitian ini adalah mengetahui kelayakan dan kepraktisan LKPD berbasis project based learning (PjBl) untuk meningkatkan keterampilan kolaborasi siswa pada materi bioteknologi siswa. Jenis penelitian ini adalah RD (Research and Development) dengan model ADDIE (Analysis, Design, Development, Implementation, Evaluation), namun penelitian ini hanya sampai pada tahap develop. Subjek uji coba dalam penelitian ini adalah 1 guru pengampu biologi, dan 7 orang perwakilan kelompok siswa. Metode pengumpulan data dengan wawancara dan angket. Data hasil penelitian dianalisis menggunakan teknik analisis kualitatif dan kuantitif. Hasil penelitian berupa: 1) dihasilkan LKPD berbasis project based learning materi bioteknologi dengan dilakukan 3 tahapan yaitu Analysis, Design, Development; 2). Penilaian dari ahli materi diperoleh skor 49 kategori “sangat layak”. Penilaian dari ahli media diperoleh skor 59 kategori “layak”. Penilaian dari guru biologi diperoleh skor 64 kategori “praktis”. Penilaian oleh 7 siswa diperoleh skor 65,14 kategori “sangat praktis”. Berdasarkan data yang didapatkan, maka LKPD project based learning pada materi bioteknologi layak digunakan untuk meningkatkan keterampilan kolaborasi siswa pada materi bioteknologi kelas X.
ISOLASI DAN IDENTIFIKASI BAKTERI TANAH SEKITAR LIMBAH MASKER MEDIS DARI MANGROVE WANA TIRTA, KULON PROGO, YOGYAKARTA Ratnasari, Tantri Ayu; Rakhmawati, Anna
Jurnal Sains Dasar Vol 13, No 2 (2024): Oktober 2024
Publisher : Faculty of Mathematics and Natural Science, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jsd.v13i2.70229

Abstract

Tujuan penelitian ini adalah untuk mengetahui karakteristik dan genus bakteri yang mampu hidup menggunakan masker medis sebagai sumber karbonnya. Penelitian ini merupakan penelitian kualitatif dengan jenis penelitian eksploratif-deskriptif yang dilakukan dengan mengeksplorisasi bakteri tanah mangrove dari sekitar limbah masker medis yang ada di kawasan ekowisata Mangrove Wana Tirta, Dusun Pasir Mendit, Desa Jangkaran, Kecamatan Temon, Kabupaten Kulon Progo, Provinsi Daerah Istimewa Yogyakarta. Isolasi dilakukan dengan menggunakan media Luria Bertani (LB) yang mengandung banyak nutrisi yang diperlukan oleh bakteri. Isolat kemudian diseleksi menggunakan Minimum Salt Media (MSM) dan ditambahkan potongan masker medis biru sebagai satu-satunya sumber karbon serta dicek nilai OD-nya. Isolat hasil seleksi selanjutnya dikarakterisasi untuk didapatkan ciri-ciri fenotipenya berupa pengamatan mikroskopis, makroskopis, uji biokimia, dan uji fisiologis. Hasil karakter digunakan untuk profil matching dengan genus bakteri acuan dan didapatkan dendogram yang menunjukkan hubungan kekerabatan dengan nilai similaritas tertentu. Berdasarkan hasil penelitian, didapatkan 10 isolat yang menunjukkan kenaikan nilai OD. Isolat tersebut diduga memiliki hubungan kekerabatan dengan 8 genus bakteri dengan nilai similaritas 70%, yakni Arthrobacter (isolat 10), Azotobacter (isolat 21), Chelatococcus (isolat 23), Enterococcus (isolat 9 dan 17), Neisseria (isolat 26), Paracoccus (isolat 11), Pseudomonas (isolat 20), dan Serratia (isolat 2 dan 29).
Website-Based Learning Media in High School Biology Learning on Genetic Substance Material Ega Safitri; Anna Rakhmawati
Jurnal Penelitian dan Pengembangan Pendidikan Vol. 8 No. 3 (2024): October
Publisher : LPPM Undiksha

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23887/jppp.v8i3.79445

Abstract

Genetic substance material requires learning media in its delivery because it cannot be seen directly, making it difficult for students to understand. This study aims to create a website-based learning media suitable for high school biology learning on genetic substance material. The research method used is research and development (R&D) with the ADDIE model. The study subjects included material and media experts, teachers, and 13 grade XII high school students. Data collection was carried out using interview, observation, and questionnaire methods. Data analysis techniques used qualitative and quantitative analysis. The results of the study showed that the validation of material experts obtained a score of 100% (very feasible), media experts obtained a score of 87.50% (very possible), individual trials obtained a score of 100% (very possible), and limited trials obtained a score of 85.79% (very feasible). It was concluded that website-based learning media is suitable for application in biology learning on genetic substance material. The implications of this study are to motivate teachers to use media in the learning process at school.
Co-Authors Abdullah Nashih Amin Ajeng Septiana Putri Aji Suhandy Althaaf, Nabila Tsabita Arifudin Idrus Astuti Astuti Aulia, Fera Ayu Natasya FR BAHARUDIN, JALU BAHTIAR Baso Samsu Rijal Bernadetta Octavia Bidhari Pidhatika Budiwati Budiwati David Meilana Nur Arifin Dhiya Millatika Tohiri Drajat Pramiadi Dwi Ratnawati, Dwi Efendi, Adhan Ega Safitri Eli Rohaeti Endang W Laksono Endang WLFX Eny Purwanti Evy Yulianti Ferella*, Elsa Fitri Rahmawati Hajidah Musyayyadah Hanifa Rizky Rahmawati Intan Lestari Isroni, Muhson Istiana Prihatini Kartika Ratna Pertiwi Kun Sri Budiasih Kurnia Cahyani Lili Sugiyarto Lili Sugiyarto Linda, Evi Marfuatun, Marfuatun Muhammad Anees Novian Indah Sari, Tasya Nrangwesthi Widyaningrum Nunuk Purwanti Nur Fathurahman Ridwan Nur Rizki Putri Ramadhanti Paidi Paskariani, Angela Enggar Pertiwi, Melania Citra Pramesty, Alfinda Ratnasari, Tantri Ayu Ria Dewi Dili Hastuti Ria Dewi Dili Hastuti Rindiana, Danny Ria Rizka Apriani Putri Sajid Iqbal Sari, Tasya Novian Indah Setiawan, Fauzi Fandy Simbolon, Doly Siti Umniyati Siti Umniyatie Siti Umniyatie Siti Umniyatie SRI ATUN Suhartini Suhartini Suhartini Suhartini Suhartini Sujangka, Arsyad Tasya Novian Indah Sari Tien Aminatun Tutiek Rahayu Wahyu Nuryadi H Yulianti Yulianti Yuliati Yuliati Yuni Wibowo