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All Journal VALENSI Jurnal Penelitian Saintek Jurnal Ilmu Lingkungan Jurnal Penelitian dan Pengembangan Pendidikan Jurnal Sains Dasar Journal of Degraded and Mining Lands Management Jurnal Penelitian Pendidikan IPA (JPPIPA) ALCHEMY Jurnal Penelitian Kimia Jurnal Pengabdian Masyarakat MIPA dan Pendidikan MIPA Molekul: Jurnal Ilmiah Kimia Journal of Innovation in Educational and Cultural Research JIPB (Jurnal Inovasi Pembelajaran Biologi) Proceeding Biology Education Conference Kaunia: Integration and Interconnection of Islam and Science Journal Research and Development in Education (RaDEn) Jurnal Natural Jurnal Pendidikan Sains Indonesia (Indonesian Journal of Science Education) Jurnal Edukasi Biologi Kingdom (The Journal of Biological Studies) Quagga: Jurnal Pendidikan dan Biologi IJOBI Didaktika Biologi: Jurnal Penelitian Pendidikan Biologi
Anna Rakhmawati
Biology Education Department, Faculty Of Mathematics And Natural Science, Yogyakarta State University, Indonesia
Religion Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Energy Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Physics Public Health Social Sciences Other

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Pelatihan Pembuatan Aneka Produk Olahan Cabai dan Bawang Merah sebagai Upaya Peningkatan Nilai Jual Hasil Pertanian dan Pemberdayaan Ibu Rumah Tangga Di Kalurahan Bugel, Kapanewon Panjatan, Kulonprogo, DIY Tien Aminatun; Fitri Rahmawati; Anna Rakhmawati; Rizka Apriani Putri; Budiwati Budiwati; Tutiek Rahayu
Jurnal Pengabdian Masyarakat MIPA dan Pendidikan MIPA Vol 7, No 2 (2023): Vol 7, No 2 (2023)
Publisher : Yogyakarta State University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jpmmp.v7i2.59846

Abstract

Bugel is one of districts in Kulon Progo Regency that uses Surjan Method in planting rice  as their local agricultural system. One of major problems faced by the farmer during harvest season is the drop of the yields’price which then lead to the financial loss for the farmers. In order to anticipate the same issue happens in the next harvest season, farmers in subdistrict Bugel, District of Panjaten, Kulon progo Regency want to have a training in regard to process the food such as Red Chilli and Shallot,   therefore rotten and damaged yields due to the longer time of storage can be reduced or even avoided. After being processed through the postharvest techniques, those yields are expected to increase in prices thus they will bring profit and more income for the citizens. This training aims to introduce postharvest techniques that can be used to store the yields longer and also to train citizens on food processing  which resulted in finer product and  ultimately can be sold in  higher price. Red Chilli and Shallots are the main yields harvested during the harvest season outside Rice. Two types of Sambal made from red Chilli and processed food of Shallots (Fried Shallot) are made during the practice. These products hopefully will last longer in storage and also can be sold in higher price compared to the fresh ones.Customer satisfactory surveys results showed that attendance were responded positively towards the training. Based on the evaluation, citizens wish for  similar training in the future with topic regarding the health and safety of the products as well as the marketing aspect of them.
Keanekaragaman Cacing Tanah Pada Lahan Sawah Tercemar Residu Pestisida Nur Rizki Putri Ramadhanti; Tien Aminatun; Anna Rakhmawati; Bernadetta Octavia; Suhartini Suhartini
Jurnal Sains Dasar Vol 12, No 1 (2023): April 2023
Publisher : Faculty of Mathematics and Natural Science, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jsd.v12i1.58953

Abstract

Penelitian ini bertujuan mengetahui kondisi fisikokimia dan keanekaragaman cacing tanah pada lahan sawah tercemar residu pestisida di Wonokerto, Turi, Sleman, Yogyakarta. Penelitian ini merupakan jenis penelitian observasi yang mendeskripsikan keanekaragaman cacing tanah di lahan tercemar residu pestisida terutama golongan organofosfat. Lahan penelitian meliputi lahan A dengan umur penggunaan pestisida organofosfat lebih dari 1 tahun dan lahan B berumur kurang dari 1 tahun. Pengambilan sampel menggunakan teknik handsorting pada petak 25x25x25cm. Sampel diidentifikasi di Laboratorium Lingkungan FMIPA UNY. Analisis data secara deskriptif berdasarkan hasil indeks keanekaragaman Shannon–Wienner. Hasil uji kandungan pestisida golongan organofosfat lahan A dan lahan B adalah tidak terdeteksi. Namun berdasarkan paparan petani, lahan A dan lahan B menggunakan pestisida golongan organofosfat dengan bahan aktif profenofos selama lebih dari 1 tahun. Untuk itu, ke dua lahan diperkirakan tercemar pestisida golongan organofosfat. Hasil uji fisikokimia tanah lahan A bertekstur geluh pasiran dengan kandungan bahan organik dan C organik lebih rendah serta pH, KTK, N total, P tersedia, dan K tersedia lebih tinggi dibanding lahan B bertekstur pasir geluhan. Tingkat keanekaragaman cacing tanah adalah rendah pada ke dua lahan dengan indeks keanekaragaman tertinggi 0,617 pada lahan B berumur kurang dari 1 tahun penggunaan pestisida golongan organofosfat.Kata kunci: keanekaragaman, cacing tanah, cemaran, residu pestisida.This research aims to discover physicochemical conditions of soil and diversity of earthworms in fields contaminated pesticide residues in Wonokerto, Turi, Sleman, Yogyakarta. This research is observational research describes earthworms’ diversity in fields contaminated pesticide residues, especially organophosphate. Research area includes field A, with exposure to organophosphate pesticides for more than 1 year, and field B less than 1 year. The handsorting was employed as a sampling technique in plots of 25x25x25cm. Samples were identified at Environmental Laboratory FMIPA UNY. Data analysis was conducted descriptively based on results Shannon–Wienner diversity index. The test results show pesticide organophosphate content in two fields was undetected. However, based on the explanation from farmers in field A and B, used organophosphate pesticides with active ingredient profenophos more than 1 year. It is estimated, both fields will remain contaminated organophosphate pesticides. The soil physicochemical tests indicate field A, a sandy loam texture, had lower organic matter content and organic C with higher pH, CEC, total N, available P, and available K compared to field B with a loamy sand texture. Diversity level of earthworms is low in both fields with highest index being 0,617 in field B, less than 1 year using organophosphate pesticides.Keywords: diversity, earthworms, contamination, pesticide residue.
Assessing soil bacterial community response to organophosphate pesticides in agricultural field of Yogyakarta, Java, Indonesia ANNA RAKHMAWATI; BERNADETTA OCTAVIA; SUHARTINI SUHARTINI; TIEN AMINATUN
Jurnal Natural Volume 23 Number 3, October 2023
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v23i3.31263

Abstract

Soil contamination by pesticides is one of the world’s most pressing environmental issues. The widespread use of Organophosphate pesticides (OPPs) in agriculture has led to biological diversity changes. The indigenous bacterial community played significant roles in the remediation of soil contaminated with OPPs. This study examines the overall bacterial community composition of three agricultural fields in Yogyakarta, Java, Indonesia, that were exposed to OPPs. The agricultural field was divided into zones near the beach, residential, and mountainous. Sequencing 16S rRNA amplicon fragments used to analyze the soil bacterial community. It was discovered that Proteobacteria, Actinobacteria, and Firmicutes comprised the majority of the bacterial community. In addition, the samples contain a high relative abundance of Bacillus, Bradyrhizobium, Chryseobacterium, Cystobacter, Microvirga, and Burkholderia. The high alpha diversity indexes suggest that the agricultural soil microbiome provides important ecological services and may harbor a wide variety of bacteria and genes with biotechnological applications. The physicochemical soil characteristics are also correlated with the bacterial community structure. The findings can be used to develop bioremediation strategies that employ native microbes to clean and restore agricultural soil contaminated with OPPs.
Identifikasi Logam Berat Kromium di Tiga Sungai yang Melintasi Kota Yogyakarta dan Potensi Fitoremediasinya Tien Aminatun; Anna Rakhmawati; Kun Sri Budiasih; Marfuatun Marfuatun; Baso Samsu Rijal; Abdullah Nashih Amin; David Meilana Nur Arifin; Ajeng Septiana Putri
Jurnal Ilmu Lingkungan Vol 22, No 3 (2024): May 2024
Publisher : School of Postgraduate Studies, Diponegoro Univer

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jil.22.3.620-631

Abstract

Winongo, Gajah Wong, dan Code adalah tiga sungai yang melintasi Yogyakarta. Banyak industri yang membuang limbah ke ketiga sungai ini, sedangkan Cr adalah logam berat yang banyak terdapat pada limbah industri. Berbagai penelitian telah dilakukan untuk melihat potensi fitoremediasi Cr, tetapi umumnya pada limbah cair industri, bukan pada perairan langsung. Tujuan penelitian ini adalah mengidentifikasi logam berat Kromium (Cr) di ketiga sungai serta potensi fitoremediasinya. Sampel air dan sedimen diambil dari hulu, tengah dan hilir, kemudian kandungan Cr dianalisis dengan Spektrofotometri UV-Vis di Laboratorium Kimia Fakultas MIPA Universitas Negeri Yogyakarta (UNY). Untuk melihat potensi fitoremediasinya dilakukan eksperimen dengan eceng gondok (Eichornia crassipes) dan kayu apu (Pistia stratiotes) di greenhouse FMIPA UNY. Pengukuran kualitas air diujikan ke Laboratorium BBTKL-PP. Hasil penelitian menunjukkan ketiga sungai teridentifikasi cemaran Cr tetapi masih di bawah ambang baku mutu menurut Peraturan Gubernur DIY Nomor 20 Tahun 2008 tentang Baku Mutu Air di Provinsi DIY Kelas 1. Potensi eceng gondok dan kayu apu sebagai fitoremediator air sungai yang tercemar Cr tidak tampak jelas karena konsentrasi Cr pada ketiga sungai masih rendah di bawah ambang baku mutu (<0.05 mg/L), akan tetapi dilihat dari performa tanaman maka kedua gulma ini berpotensi untuk menjadi fitoremediator di sungai yang tercemar Cr.
Analysis the quality of critical thinking and creativity questions in high school biology subjects with the Rasch model Tasya Novian Indah Sari; Anna Rakhmawati
Research and Development in Education (RaDEn) Vol. 4 No. 1 (2024): July
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/raden.v4i1.32758

Abstract

A good instrument is an instrument that can measure students' abilities accurately. The research aims to analyze the quality of critical thinking and creativity items on water pollution material in terms of validity, reliability, and level of difficulty using the Rasch model. The research used a quantitative descriptive method involving biology learning experts and 173 class X high school students. The research was carried out in December 2023-January 2024. The data collection method used expert assessment sheets and critical thinking and creativity essay questions. Data were analyzed using the Rasch model assisted by Quest. The results showed that of the 10 critical thinking essay questions, 3 questions were invalid, while of the 8 creativity essay questions, 1 question was invalid based on the suitability of the INFIT MNSQ and OUTFIT T scores. The results of the reliability test with Cronbach’s alpha showed that the reliability value of the critical thinking instrument was 0.74 and creativity 0.79. Average difficulty level of questions with threshold values in the medium and difficult categories. Overall, the instrument was feasible and question items that did not fit the Rasch model were removed.
IDENTIFIKASI MATERI SULIT KOMPETENSI DASAR BAKTERI PADA SISWA KELAS X SEMESTER I DI SMA NEGERI 1 KOTA MUNGKID Rindiana, Danny Ria; Rakhmawati, Anna
Jurnal Edukasi Biologi Vol 8, No 2 (2022): Jurnal Edukasi Biologi
Publisher : Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/edubio.v8i2.18386

Abstract

Penelitian ini bertujuan untuk mengetahui letak materi sulit kompetensi dasar bakteri pada siswa kelas X Semester I di SMA Negeri 1 Kota Mungkid Tahun Ajaran 2021/2022 berdasarkan submateri. Penelitian ini merupakan penelitian deskriptif. Sampel pada penelitian ini ditentukan dengan teknik cluster sampling. Instrumen penelitian berupa soal tes kompetensi dasar bakteri. Analisis data dilakukan dengan menghitung ketercapaian belajar siswa kemudian dibandingkan dengan kriteria tingkat kesulitan materi menurut teori. Hasil Penelitian menunjukkan letak materi sulit kompetensi dasar bakteri berdasarkan submateri adalah pada submateri karakteristik bakteri, klasifikasi bakteri dan reproduksi bakteri dengan tingkat kesulitan sukar. Sub materi peran bakteri dan definisi bakteri memiliki tingkat kesulitan sedang. Urutan materi sulit dari tinggi ke rendah adalah submateri karakteristik bakteri, klasifikasi bakteri, reproduksi bakteri, peran bakteri dan definisi bakteri.
ISOLASI, KARAKTERISASI, DAN IDENTIFIKASI BAKTERI HALOFILIK PENGHASIL PROTEASE DARI IKAN ASIN LAYUR (Trichiurus lepturus) DI PASAR BERINGHARJO YOGYAKARTA Pramesty, Alfinda; Rakhmawati, Anna
Kingdom: The Journal of Biological Studies Vol 9, No 2 (2023): Kingdom: The Journal of Biological Studies
Publisher : Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/kingdom.v9i2.18363

Abstract

Pembuatan ikan asin layur (Trichiurus lepturus) menggunakan ikan air laut dari genus Trichiurus yang berpotensi mengandung bakteri halolifik. Ikan asin memiliki potensi besar sebagai sumber biomassa penghasil enzim protease yang tahan terhadap kadar garam tinggi (halostabil). Tujuan dari penelitian ini untuk mengetahui untuk mengetahui karakteristik dan genus, serta hubungan kekerabatan antar isolat bakteri halofilik penghasil protease dari ikan asin layur (T. Lepturus) di Pasar Beringharjo Yogyakarta. Penelitian ini merupakan penelitian deskriptif eksploratif dengan metode observasi. Pengambilan sampel ikan asin layur (T. lepturus) dilakukan pada 3 kios pedagang berbeda berdasarkan daerah hasil produksi pengolahan ikan asin tersebut. Sampel selanjutnya diisolasi pada media selektif Skim Milk Agar + 3% NaCl untuk menumbuhkan bakteri halofilik penghasil protease. Isolat bakteri yang didapatkan kemudian dimurnikan dan dilukan karakterisasi fenetik meliputi morfologi koloni dan sel, fisiologis (biokimia), serta kemampuan tumbuh pada kadar NaCl 0%, 10%, dan 20%. Data yang diperoleh digunakan untuk identifikasi dengan metode matching profile berdasarkan Bergey’s Manual of Determinative Bacteriology. Hubungan kekerabatan antar isolat bakteri diklasifikasikan menggunakan software MVSP 3.1 dengan algoritma pengklasteran UPGMA dan dikonstruksikan dalam bentuk dendogram. Hasil penelitian menunjukkan terdapat 7 isolat bakteri halofilik penghasil protease dari ikan asin layur (T. lepturus) yang berhasil diisolasi dari kios pedagang berbeda yaitu 3 isolat berasal dari kios A, 3 isolat berasal dari kios B, dan 1 isolat berasal dari kios C. konstruksi dendogram menunjukan 9 klaster bakteri. Terdapat 2 isolat bakteri yang memiliki indeks similaritas ≥ 83% terhadap acuan Staphylococcus sp. yaitu isolat A5 dan C6. Sebanyak 4 isolat bakteri yang memiliki indeks similaritas ≥ 67% terhadap Salinococcus sp. yaitu A6, A8, B5, dan B6. Serta 1 isolat bakteri yang memiliki indeks similaritas 83% terhadap strain acuan Halomonas sp. yaitu isolat B6.Kata kunci : Isolasi;Bakteri Halofilik;Proteolitik;Karakter FenetikIsolasi, Bakteri Halofilik, Proteolitik, Karakter Fenetik.
Lithogeochemical characteristics and potential hyperaccumulator identification as phytomining agent at the Ratatotok gold mine, Indonesia Aminatun, Tien; Idrus, Arifudin; Simbolon, Doly; Rakhmawati, Anna; Atun, Sri
Journal of Degraded and Mining Lands Management Vol. 11 No. 2 (2024)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15243/jdmlm.2024.112.5251

Abstract

In the future, phytomining will be an environmentally friendly alternative mining technology. Therefore, the exploration of vegetation types having the potential as hyperaccumulators in gold phytomining needs to be carried out. This study aimed to (1) investigate the effect of rock/soil mineralogy characteristics and Au content on the diversity of vegetation types at gold mine sites, and (2) determine the type of potential hyperaccumulator vegetation as a phytomining agent based on the Biological Concentration Factor (BCF). This study was conducted at the Ratatotok gold mine in North Sulawesi Province, Indonesia. The sampling locations consisted of 3 sites, i.e., Bulex, Yance, and Leon, and each site consisted of 5 sampling plots. Soil samples were taken from each sampling plot and then tested for gold content using the ICP-MS method and mineral content using the XRD method. Mineralized bedrock samples were also taken for mineralogical analysis through petrography. Data analysis of soil geochemistry was carried out descriptively. Quantitative descriptive analysis was also carried out to determine the dominant type of vegetation, which was potential for hyperaccumulators at the mine site. The results showed that gold content in the soil affected the diversity of vegetation, which was possibly due to gold stress, which affected plant growth. Based on the BCF value, three local plant species having potential as gold hyperaccumulators with moderate bioaccumulation ability (BCF>0.1-1) were found, namely Pteris vittata, Syzygium aromaticum, and Swietenia mahagoni. However, the use of these plants as phytomining agents requires further research.
Development of PjBL-based e-worksheets utilizing the kombucha bioprocess in high school biology learning Dhiya Millatika Tohiri; Anna Rakhmawati
Research and Development in Education (RaDEn) Vol. 4 No. 2 (2024): December
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/raden.v4i2.36263

Abstract

Worksheets are still often made in the format of questions that are done individually. Kombucha, when viewed from the characteristics of the manufacturing process, have the potential to be innovations in conventional biology technology innovation learning. This study aimed to assess the feasibility and practicality of using project-based learning electronic worksheets with kombucha bioprocess for teaching biology. The research method employed was development research using the ADDIE model, focusing on the analysis, design, and development stages. The study involved one material expert, one media expert, two biology teachers, and 33 students. Data was collected using interviews and questionnaires. The electronic worksheet was evaluated by media and material expert lecturers for feasibility and by teachers and students for practicality The results indicated that this worksheet is both feasible and practical for biology learning activities with a material validity level of 72.6% (feasible), media validity of 98% (very feasible), biology teacher response 92% (very practical) and student response 85% (very practical). The findings of this study are expected to be an inspiration for educators in compiling media for learning activities, becoming the latest and most interesting learning content innovations in project creation.
PENGEMBANGAN LKPD BERBASIS PROJECT BASED LEARNING UNTUK MENINGKATKAN KETERAMPILAN KOLABORASI SISWA MATERI BIOTEKNOLOGI Lestari, Intan; Rakhmawati, Anna
Jurnal Edukasi Biologi Vol 10, No 2 (2024): Jurnal Edukasi Biologi
Publisher : Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/edubio.v10i2.19644

Abstract

Abstrak. Tujuan penelitian ini adalah mengetahui kelayakan dan kepraktisan LKPD berbasis project based learning (PjBl) untuk meningkatkan keterampilan kolaborasi siswa pada materi bioteknologi siswa. Jenis penelitian ini adalah RD (Research and Development) dengan model ADDIE (Analysis, Design, Development, Implementation, Evaluation), namun penelitian ini hanya sampai pada tahap develop. Subjek uji coba dalam penelitian ini adalah 1 guru pengampu biologi, dan 7 orang perwakilan kelompok siswa. Metode pengumpulan data dengan wawancara dan angket. Data hasil penelitian dianalisis menggunakan teknik analisis kualitatif dan kuantitif. Hasil penelitian berupa: 1) dihasilkan LKPD berbasis project based learning materi bioteknologi dengan dilakukan 3 tahapan yaitu Analysis, Design, Development; 2). Penilaian dari ahli materi diperoleh skor 49 kategori “sangat layak”. Penilaian dari ahli media diperoleh skor 59 kategori “layak”. Penilaian dari guru biologi diperoleh skor 64 kategori “praktis”. Penilaian oleh 7 siswa diperoleh skor 65,14 kategori “sangat praktis”. Berdasarkan data yang didapatkan, maka LKPD project based learning pada materi bioteknologi layak digunakan untuk meningkatkan keterampilan kolaborasi siswa pada materi bioteknologi kelas X.
Co-Authors Abdullah Nashih Amin Ajeng Septiana Putri Aji Suhandy Althaaf, Nabila Tsabita Aminatun, Tien Arifudin Idrus Astuti Astuti Aulia, Fera Ayu Natasya FR BAHARUDIN, JALU BAHTIAR Baso Samsu Rijal Bernadetta Octavia Bidhari Pidhatika Budiwati Budiwati David Meilana Nur Arifin Dhiya Millatika Tohiri Drajat Pramiadi Dwi Ratnawati, Dwi Efendi, Adhan Ega Safitri Eli Rohaeti Endang W Laksono Endang Widjajanti Laksono FX Endang WLFX Eny Purwanti Evi Linda Evy Yulianti Ferella*, Elsa Fitri Rahmawati Hafizh Adyatma Hajidah Musyayyadah Hanifa Rizky Rahmawati Intan Lestari Isroni, Muhson Istiana Prihatini Kartika Ratna Pertiwi Kun Sri Budiasih Kurnia Cahyani Lili Sugiyarto Marfuatun . Muhammad Anees Novian Indah Sari, Tasya Nrangwesthi Widyaningrum Nunuk Purwanti Nur Fathurahman Ridwan Nur Rizki Putri Ramadhanti Paidi Paskariani, Angela Enggar Pertiwi, Melania Citra Pramesty, Alfinda Rania Nur Farida Ratnasari, Tantri Ayu Ria Dewi Dili Hastuti Ria Dewi Dili Hastuti Rindiana, Danny Ria Rizka Apriani Putri Sajid Iqbal Sari, Tasya Novian Indah Setiawan, Fauzi Fandy Simbolon, Doly Siti Umniyati Siti Umniyatie Siti Umniyatie Siti Umniyatie SRI ATUN Suhartini Suhartini Suhartini Suhartini Suhartini Sujangka, Arsyad Tasya Novian Indah Sari Tutiek Rahayu Wahyu Nuryadi H Yulianti Yulianti Yuliati Yuliati Yuni Wibowo