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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Agrointek Jurnal Agroteknos Jurnal Sains dan Teknologi Pangan PROSIDING SEMINAR NASIONAL AGRIBISNIS Berkala Penelitian Agronomi Akta Agrosia PEDAMAS (Pengabdian Kepada Masyarakat)
ANDI KHAERUNI
Universitas Haluoleo, Kendari 93232
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Education Energy Engineering Health Professions Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Social Sciences

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ISOLASI Bacillus thuringiensis BERL. DARI TANAH DAN PATOGENISITASNYA TERHADAP LARVA Crocidolomia binotalis ZELL. PADA TANAMAN SAWI (Brassica juncea L.) Khaeruni, Andi; Rahayu, M; Purnamaningrum, Nenden Teja
Jurnal Agroteknos Vol 2, No 1 (2012)
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The aim of this research was to know the pathogenicity of B. thuringiensis against larvae of   C. binotalis Zell on Caisin Plant. The B. thuringiensis isolates were isolated using serial dilution technique, from soil samples that were taken from some locations in Southeast Sulawesi. Pathogenicity test on larvae III of C. binotalis was done using bioassay method. Mortality value was used to know the virulence level of  B. thuringiensis at 7 days after application. The result showed that there were 37 soil samples containing B. thuringiensis and the number of B. thuringiensis isolates were 65. isolates KU-ST7, MN-ST4, KNW-ST8, KLK- ST5,KLK-ST4 and KU-ST4 had the highest pathogenicity and virulence levels, because they had the highest efffect on C. binotalis larvae mortalities, i.e: 100%, with  LT50  value of 2-4  days after application. Key word : Bacillus thuringiensis Berl., bioassay test. Crocidolomia binotalis Zell.,
ISOLASI, KARAKTERISASI DAN UJI ANTAGONIS BAKTERI SELULOLITIK TERHADAP Phytophthora capsici ASAL TANAMAN LADA (Piper nigrum L.) SECARA IN-VITRO Khaeruni, Andi; Satrah, Vit Nehru; Mariadi, Mariadi
Jurnal Agroteknos Vol 1, No 3 (2011)
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ABSTRACT The Aim of this experiment was obtain cellulolitic bacteria that has antagonistic potential toward P. capsici of   pepper isolated under in-vitro test.   The experiment was begun in isolation and selection of cellulolitic bacteria from soil samples were taken from some location at Southeast Sulawesi. Selected 20 isolates of cellulolitic bacteria examined for its    morphological and physiological characteristics as  well as antagonistic potential toward   P. capsici of pepper isolated under in-vitro test on Potato Dextrose Agar (PDA) medium. The potential antagonistic test was carry out used randomized complete design with three replications to observes the ability of cellulolitic bacteria inhibited mycelium growth of P. capsici on PDA medium. Results showed the selected isolates very divers on morphological characters in  size, pigmentation, shape,  and  elevation of  surface. Physiological character showed  that  out  of  20  isolates,  12  isolates  have  negative  Gram reaction  and  8  isolates    positive  Gram  reaction.  Obtaining  5  isolates  very  strongly,  11 isolates strongly and 4 isolates weak activities on secreted extracellular cellulose enzyme, all of isolates were facultative anaerobe activity.  Further test on antagonistic ability showed that STS15c and STS12c2 isolates are the best isolates to inhibited mycelium growth of P. capsici on PDA medium in potential inhibited were 53.16% and 52.02% respectively. Key words: Cellulolitic bacteria, Phytophthora capsici, antagonistic
PENGENDALIAN HAYATI PENYAKIT LAYU FUSARIUM PADA TANAMAN TOMAT (Solanum lycopersicum L.) MENGGUNAKAN BAKTERI ENDOFIT ASAL TANAMAN LIAR NERU SATRAH, VIT; ASDAR, ASDAR; KHAERUNI, ANDI; SANTIAJI BANDE, LA ODE; RAHMAN, ABDUL; ISNAINI ULFA, NUR; ARIYANTI, EKA LESTARI
Jurnal Agroteknos Vol 13, No 3 (2023)
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This research aims to determine the effect of using endophytic bacteria from wild plants in the biological control of Fusarium Wilt disease on tomato plants and to find out which isolate method is best for controlling Fusarium Wilt disease on tomato plants. The research was carried out at the Plant Protection Laboratory, Phytopathology Unit, and Experimental Garden Greenhouse 1, Faculty of Agriculture, Halu Oleo University, Kendari, from September to November 2022. This research used a Randomized Block Design (RBD) with 5 treatments repeated 3 times for a total of 15 treatment units. The variables observed were divided into 2, namely vegetative plant growth (plant height, number of leaves, and number of productive branches) and the incidence of Fusarium Wilt disease on tomato plants. The data was analyzed using variance and if there was a real influence, it was continued with the Duncan Multiple Range Test (DMRT) at a confidence level of 95%. The results of the research showed that endophytic bacteria from wild plants were able to increase plant growth and control Fusarium Wilt disease in tomato plants and ASR-3(2) isolate was the best isolate in increasing plant growth and controlling Fusarium Wilt disease.
JENIS DAN INTENSITAS KERUSAKAN HAMA UTAMA TANAMAN CABAI PADA SISTEM BUDIDAYA YANG BERBEDA SATRAH, VIT NERU; DARLAN, DARLAN; M., RAHAYU; SYAIR, SYAIR; KHAERUNI, ANDI; MARIADI, MARIADI; RAHMAN, ABDUL; PAKKI, TERRY; ANAS, ARSY AYSYAH
Jurnal Agroteknos Vol 12, No 3 (2022)
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The research aims to determine the main types of pests and the intensity of their damage to chili plants with different cultivation systems. This research was conducted in Konda Satu Village, Konda District, South Konawe Regency. This study consisted of 3 treatments with 6 replications, so there were 18 experimental units. Observational data were analyzed using analysis of variance (ANOVA) and if the results showed significant differences then it was continued with the Least Significant Difference Test (LSD) at the 95% confidence level to find out the best treatment by testing the differences between all treatments. Then test further LSD0.05. This study used three cultivation systems as treatments, namely the Conventional Cultivation System (Without Application of Biofresh Fertilizer + 100% Inorganic recommended dose + Use of Plastic Mulch), Semi-Organic Cultivation System (Application of Biofresh Fertilizer 50% recommended dose + 50% Inorganic of recommended dose + Application Phymar C Botanical Pesticides + Use of Plastic Mulch), and Organic Cultivation Systems (100% recommended dosage of Biofresh Fertilizer Application + Phymar C Botanical Pesticide Application + Organic Mulch). The results showed that the main pests that attack chili plants are armyworm pests (Spodoptera litura), fruit fly pests (Bactrocera sp.) and Aphids pests (Aphididae). The lowest damage intensity of armyworm pests was in the organic cultivation system of 18.08%, the lowest intensity of fruit fly pest attacks was in the semi-organic cultivation system of 31.81%, the lowest intensity of attack by aphids was in the semi-organic cultivation system of 12.59%.
EKSPLORASI DAN KARAKTERISASI AZOTOBACTER INDIGENOUS UNTUK PENGEMBANGAN PUPUK HAYATI TANAMAN PADI GOGO LOKAL DI LAHAN MARJINAL NURMAS, ANDI; -, NOFIANTI; RAHMAN, ABDUL; KHAERUNI, ANDI
Jurnal Agroteknos Vol 4, No 2 (2014)
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Azotobacter is bacterium that has the ability to fix nitrogen and produce hormone IAA, thus potentially acts as biofertilizer agent. There are differences in chemical, biological and other characters of Azotobacter strains. Some strains have higher ability to fix nitrogen than the others. Exploration and characterization are important to be done because the bacteria that provide nutrients and live free rhizosphere regions, possible have different abilities.  In addition to N2 fixing, growth hormone production, phosphate dissolving, they are also tolerant to a certain temperature and pH. The purpose of the research was to obtain information and the latest data on indigenous Azotobacter that potential as a source of biofertilizer, and triggering factor for upland rice growth on marginal lands in Southeast Sulawesi.  Exploration from several locations has selected 21 Azotobacter isolates. The test results indicated that the 21 Azotobacter isolates have the ability to produce IAA, dissolve phosphate and stable at pH 5.0-7.0. All 21 isolates tested had the ability to survive at 40oC, eight isolates i.e. LT2D1, LT2d2, LU2c, RG4c, MP1f, LT2d3, ML1j, and RR8awere able to survive at a temperature of 45o C, and LT2d1 isolate survived at temperatures 50oC. The results of the evaluation of the wet weight of upland rice seedlings selected 10 isolates that were: KU6e, MS3e, RG4c, RR8b1, LU2c, RB4b, MS3f, LU2c1, RJ5e, RR8b2 and evaluation of seedling dry weight selected 5 isolates that were: RB4b, LU2c, RJ5e, RR8b2, LT2d1. Keywords:      exploration, characterization, indigenous Azotobacter, local upland rice
PENGGUNAAN PUPUK KANDANG TERHADAP EFEKTIFITAS Trichoderma viride UNTUK MENGENDALIKAN PENYAKIT LAYU FUSARIUM PADA TANAMAN TOMAT Asniah, Asniah; Khaeruni, Andi; Anwar, Hasniar
Jurnal Agroteknos Vol 2, No 1 (2012)
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Fusarium oxysporum is one of the important pathogens that causes disease in many crops, especially horticulture crops, one of which causes Fusarium wilt disease in tomato plants . The purpose of this study was to evaluate the use of manure on the effectiveness of Trichoderma viride to control fusarium wilt disease on tomato plants. Manure used was cow and chicken manure, applied before and after planting. The research design was completely randomized design (CRD) with 9 (nine) treatment and 3 replicates. The treatments were without the application of manure and T. viride (A) , without manure but with T . viride before planting (B), without manure but  with T. viride applied after planting (C), chicken manure without T. viride (D), chicken manure and T. viride application before planting (E), chicken manure and T. viride application after planting (F), cow manure without T. viride (G), cow manure and T. viride application before planting (H), cow manure and application of T. viride after planting (I). The results showed that chicken manure and T. viride application before planting were effective in reducing the incidence and severity of Fusarium wilt disease on tomato plants. The incidence and severity of disease was at 20 % and 26.67 % , respectively. Keyword: manure, tomato plants, Trichoderma viride 
RESPON KETAHANAN BERBAGAI VARIETAS TOMAT TERHADAP PENYAKIT LAYU BAKTERI (Ralstonia solanacearum) Adriani, Adriani; Rahman, Abdul; Gusnawaty, HS; Khaeruni, Andi
Jurnal Agroteknos Vol 2, No 2 (2012)
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The study was aimed to  know the resistance response of some tomato varities to bacterial wilt disease (Ralstonia solanacearum). The study was arranged in a completely randomized design (CRD) with six tomato variety treatmens, namely : Permata, Cosmonot, Idola, Gress, Ratna, and a local Muna variety. Each treatment had three replicates of six plants. The resistance response observed were : the latent period, disease incidence, disease severity,  and  plant  height.  The  response  was   observed  weekly  until  7   weeks  after inoculation.  The  results  showed  that  Cosmonot  variety  was  resistant  to  bacterial  wilt disease, without any disease incedence occured and the disease severity was only 6,66%. Whereas the Local Muna variety as well as Permata, Gress, and Idola were susceptible to bacterial wilt disease. On the other hand, Ratna variety showed slight tolerant response. Keywords : bacterial wilt, Ralstonia solanacearum, resistance reaction, tomato variety
POTENSI RIZOBAKTERI INDIGENOUS ULTISOL UNTUK MENGENDALIKAN PENYAKIT BUSUK BATANG PHYTOPHTHORA (Phytophthora capsici) PADA TANAMAN CABAI. Khaeruni, Andi; Sutariati, Gusti Ayu Kade; Rahman, Abdul
Jurnal Agroteknos Vol 1, No 1 (2011)
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The major problems to use the ultisol land in Southeast Sulawesi are low pH, poor nutrients  and  Fusarium  wilt  disease  suppression.      Plant  growth-promoting and bioprotecting rhizobacteria (PGPBR) is one of the few possibilities to overcome these problems. The objectives of this experiment were to evaluate the potency of ultisol indigenous rhizobacteria to control Phytophthora stem rot disease on chili pepper. Evaluation was conducted using five isolates of rhizobacteria (ST17c, ST26c, SS15b, SS16b & SS29a), as biological control agents treatments, compared to control treatment. The results indicated that application of ultisol indigenous rhizobacteria on seed and chili pepper rhizosfer significantly increased plant height and reduced disease incidence and disease severity of Phytophptora stem rot disease in chili pepper, compared to control. The best result was by the application of ST26c isolate, resulted in no disease symptoms developed during this experiment and significant increase in plant height to 32.28 centimeter at six week after planting. Key words: rizobacteria, Phytophthora capsici, Phytophthora stem rot disease
ISOLASI DAN KARAKTERISASI BAKTERI MANOLITIKASAL BONGGOL POHON SAGU WAHYUNI, SRI; -, LIANTO; KHAERUNI, ANDI
Jurnal Agroteknos Vol 4, No 3 (2014)
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Sago Processing in Southeast Sulawesi often generate waste in the form of pulp and tuberswhich contains lignin, cellulose, starch, minerals, and vitamins that can be used as a source of carbon and energy for growth of microorganisms, so it is likely to get microbes, including mannolitic bacteria that are useful for human life. The aim of this study was to isolate and characterize the biochemical properties of mannolitic bacteria originated fromwaste of sago hump in Konawe, Southeast Sulawesi Province. Isolation was done using serial dilution method and then spread over the surface of Nutrient agar medium and Mannan enrichment. Bacterial isolates showing high mannose activity were characterized mannose physiologically and biochemically. From this research, 6 mannolitic isolates originated from hump of sago waste samples from South Konawe were obtained. BLS.11-01 and BLS.11-02 mannolitic bacterial isolates had a strong mannolitic activity, with mannolitic index value of 2.3 and 2.0, respectively. Presumably, the two isolates were gram-positive bacteria, belonging to the same genus.
PENGELOMPOKAN PATOTIPE Xanthomonas oryzae pv. oryzae ASAL SULAWESI TENGGARA MENGGUNAKAN PADI GALUR ISOGENIK IRRI Syair, H; Samirin, Samirin; Wijayanto, Teguh; Khaeruni, Andi
Jurnal Agroteknos Vol 2, No 1 (2012)
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This Research aimed at grouping of Xanthomonas oryzae pv. oryzae pathotypes in rice cropping center of Southeast Sulawesi using isogenic lines of IRRI, as well as to know the Xanthomonas oryzae pv. oryzae pathotypes dominating and spreading in rice cropping center of Southeast Sulawesi. The pathotype grouping was determined by leaf innoculated cutting method using 30 isolates of Xanthomonas oryzae pv. oryzae from Southeast Sulawesi in 10 isogenic lines, namely IRBB1, IRBB2, IRBB3, IRBB4, IRBB5, IRBB7, IRBB10, IRBB11, IRBB13, and IRBB21. Each isolate was inoculated on the 10 isogenic lines and repeated twice. Observation  of disease intensity was conducted randomly on five leaves at four weeks after inoculation. The disease intensity was measured by the ratio of the length (cm) of leaf blight symptom to total the length of leaf sample (cm). The research results indicated there were seven pathotypes of Xanthomonas oryzae pv. oryzae in Southeast Sulawesi, i.e : pathotype X, XI, XII and four new pathotypes. Pathotype XII was the dominant pathotype, abort 79.31% of the total isolates and spreading in three regencies, Kolaka, Konawe, and South Konawe. Keywords: bacterial leaf blight, Xanthomonas oryzae pv. oryzae, pathotype
Co-Authors Abdul Madiki Abdul Rahman Adnan, Nur Sakinah ADRIANI ADRIANI Afa, Musadia Afrianti, Neli Anak Agung Istri Sri Wiadnyani andi Dahlan ANDI NURMAS, ANDI Ansharullah ansharullah Anwar Anwar Anwar, Hasniar ARIYANTI, EKA LESTARI Arsy Aysyah Anas Asdar Asdar Asmar Hasan Asniah Asniah Asrianti, Asrianti Basoka, Sri Wahyuni Botek, Muhammad Botek, Muhammad DARLAN, DARLAN Elvira, Ilian Fahmiyasin, Muhammad Fatahu, Fatahu Gusnawaty Gusnawaty GUSNAWATY HS, GUSNAWATY GUSNAWATY, HS Gusti Ayu Kade Sutariati Guyasa, I Made Hariani, Ni Kadek Dwi Hariyani HEMI, WA Hisein, Waode Siti Anima ISNAINI ULFA, NUR LA MUDI, LA La Ode Santiaji Bande LIANTO -, LIANTO M., RAHAYU Mariadi Mariadi, Mariadi Maulidah, Hikmah Muchtar, Febriana MUHAMMAD TAUFIK NERU SATRAH, VIT NOFIANTI -, NOFIANTI NOOR AIDAWATI Nur Afni PURNAMA HIDAYAT Purnamaningrum, Nenden Teja Rachmawati Hasid Radhiansyah, Muhammad Rahayu Mallarangeng, Rahayu Rahayu, M RAHIMI, ANDI SITTI Rahmahwati, Rizka Ratu, Serli Sainul, Ahmad Samirin, Samirin Saputra, Anang Andrianto SARAWA MAMMA, SARAWA Satrah, Vit Nehru Satrah, Vit Neru Satrah, Vit Neru SEDYO HARTONO Sidupa, Heron Elsa Solly Aryza Sri Wahyuni SRI WANGADI, SRI Syair Syair, H Syair, Syair Tanza Nirmala Teguh Wijayanto TERRY PAKKI, TERRY Usnul F, Maysya Sarwa Waode Siti Anima Hisein Wati, Dina Wibawa, Gusti Ngurah Adi