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Oom Komala
Universitas Pakuan
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UJI EFEKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAN EKSTRAK AIR KELOPAK BUNGA ROSELLA (Hibiscus sabdariffa L.) TERHADAP BAKTERI Streptococcus pneumoniae Oom Komala; Reni Rosyanti; Muztabadihardja Muztabadihardja
BERITA BIOLOGI Vol 12, No 1 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v12i1.519

Abstract

The in vitro study of antibacterial effectiveness of Rosella (Hibiscus sabdariffa L.) sheath ethanol extract and water extract on Streptococcus pneumoniae was conducted by diffusion and dilution method. Basic solution amounting to 70g/100 ml of the extract was diluted into 10%, 30%, 50% and 70% for diffusion test with positive control ampicillin 10 IU, and 0,1%, 0,25%, 0,5%, 1%, and 2% ethanol extract for dilution test on S. pneumoniae. The results showed that the ethanol extract and water extract could delay of bacterium growth. Agar diffusion test (disk diffusion) was observed by diameter of bar region around the disk. At concentration 70 % among extract ethanol and water extract made bar zone wide the best, the diameter mean was estimated 25.6 mm for ethanol extract, and 24.3 mm for water extract on S. pneumoniae. In dilution test, the result showed Minimum Inhibitory Concentration (MIC) of the extract was 1% on S. pneumoniae. The result of phytochemicals test is known that ethanol extract and water extract of rosella contains of saponin, tanin, and flavonoid.
EFEK AROMATERAPI MINYAK ATSIRI MAWAR (Rosa damascena MILL.) DAN KULIT JERUK LIMAU (Citrus amblycarpa) TERHADAP JUMLAH MIKROBA UDARA RUANGAN BERPENDINGIN Oom Komala; Novi Fajar Utami; Siti Mariyam Rosdiana
BERITA BIOLOGI Vol 19, No 2 (2020)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v19i2.3697

Abstract

Energy, material, and or other components were entered to the air by activities of human could cause air quality down to a specific level that could affect human health. The bacteria in the human oral cavity such as Staphylococcus sp. were found in the air during common activities such as coughing, sneezing, and speaking. The research aimed to assess the influence of the combination of oils essential of Rosa damacena and leather oil of Citrus amblycarpa against a decline in the total of microbes on air-conditioned rooms. The method of the research  was used the evaporation of aromatherapy and plate count method. The results were showed that P3 with the concentration of oils essential of R. damacena 2% and leather oil of C. amblycarpa 5% most effective could decrease the total of microbes with an average of 72.5%. Organoleptic, relative density, refractive index, solubility in 90% ethanol, and acid number analysis of Rosa damacena and Citrus amblycarpa leather essential oils met the Indonesian National Standard requirements except for the acid number of leatherlime essential oil and the optical rotation of rose essential oil. It is concluded that the combination of oils essential R. damacena and leather oil of C. amblycarpa  could reduce the total of microbes on air-conditioned rooms.
UJI EFEKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAN EKSTRAK AIRKELOPAK BUNGA ROSELLA (Hibiscus sabdariffa L) TERHADAP BAKTERI Streptococcus pneumoniae. Oom Komala; Reni Rosyanti; Muztabadihardja Muztabadihardja
FITOFARMAKA: Jurnal Ilmiah Farmasi Vol 3, No 1 (2013): FITOFARMAKA
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (374.109 KB) | DOI: 10.33751/jf.v3i1.173

Abstract

Penelitian efektivitas antibakteri ekstrak etanol dan ekstrak air kelopak bunga rosella terhadap Streptococcus pneumoniae telah dilakukan menggunakan metode difusicakram dan dilusi secara invitro. Larutan induk ekstrak etanol dan ekstrak air rosella70g/100 ml diencerkan dengan konsentrasi 10%, 30%, 50% dan70% untuk metodedifusi cakram dengan kontrol positif ampisilin 10 IU, dan ekstrak etanol diencerkandengan konsentrasi 0,1%, 0,25%, 0,5%, 1%, dan 2% untuk metode dilusi terhadapS.pneumoniae. Hasil penelitian menunjukkan bahwa ekstrak etanol dan ekstrak airrosella dapat menghambat pertumbuhan bakteri S.pneumoniae diketahui dari zonahambat yang terbentuk. Konsentrasi 70% pada metode difusi ekstrak etanol danekstrak air rosella membentuk diameter zona hambat paling luas rata-rata 25,6 mmuntuk ekstrak etanol dan 24,3 mm untuk ekstrak air rosella. Hasil konsentrasi hambatminimum dari ekstrak etanol rosella yang paling efektif adalah konsentrasi 1%. Hasilpengujian fitokimia diketahui bahwa ekstrak etanol dan ekstrak air rosellamengandung saponin, tanin, dan flavonoid.Kata kunci : Kelopak Bunga Rosella (Hibiscus sabdariffa L), S.pneumoniae, antibakteri,ekstrak etanol, ekstrak air
UJI AKTIVITAS EKSTRAK ETANOL 96% DAN FRAKSI DAUN KIRINYUH (Chromolaena odorata L.) TERHADAP PROPIONIBACTERIUM ACNES Oom Komala; Yulianita .; Rita Rahmawati
FITOFARMAKA: Jurnal Ilmiah Farmasi Vol 11, No 1 (2021): FITOFARMAKA | Jurnal Ilmiah Farmasi
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/jf.v11i1.2657

Abstract

Chromolaena odorata L. leaves can be used as an antibacterial because of contain flavonoid compounds. The secondary metabolite content of Chromolaena odorata L leaves include flavonoids, tannins, saponins, and steroids which have antibacterial activity. The aim of this study was determine the antibacterial activity of  ethanolic 96% and fraction of Chromolaena odorata L leaves and determine the concentration of  Chromolaena odorata L fraction and ethanol 96% extract which was most effective as an antibacterial against Propionibacterium acnes. The method was used to determine the MIC was a solid dilusion method, while the determination of inhibitory zone by the paper disk diffusion method at concentrations of 10%, 15% and 20% in each sample. The MIC showed hexane fraction is 15%, ethylacetate fraction is 15% and water fraction is 10%. The best antibacterial activity in the ethylacetate fraction and ethanol 96% extract  at a concentration of 20% with the width of the zone of inhibition values of 4.375 mm and 4 mm.
UJI EFEKTIVITAS EKSTRAK ETANOL DAUN LIDAH MERTUA (Sansevieria trifasciata Prain) TERHADAP KHAMIR Candida albicans Oom Komala; Ike Yulia; Rita Pebrianti
FITOFARMAKA: Jurnal Ilmiah Farmasi Vol 2, No 2 (2012): FITOFARMAKA
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (604.399 KB) | DOI: 10.33751/jf.v2i2.169

Abstract

Lidah mertua (Sansevieria trifasciata Prain) merupakan tanaman yang berasal dariAfrika dan dikenal sebagai antimikroba, serta berkhasiat obat. Tujuan dari penelitian iniialah mengetahui kandungan antimikroba ekstrak daun lidah mertua dengan menentukanlebar daerah hambat (LDH) terhadap khamir Candida albicans menggunakan metode difusikertas cakram. Pengujian LDH dilakukan terhadap konsentrasi ekstrak daun lidah mertua60%, 70% , 80%, 90%, serta ketokonazol 14 ppm sebagai kontrol positif dan karboksi metilselulosa (CMC) 0,5% sebagai kontrol negatif. Hasil penelitian menunjukkan bahwa ekstrakdaun lidah mertua pada konsentrasi 90% membentuk zona hambat terhadap C. albicansyang paling luas tetapi tidak jernih. Hasil analisis mutu ekstrak diketahui bahwa kadar abu ekstrak daun lidah mertua yang tidak larut dalam asam ialah 0,23% dan yang larut dalamair ialah 5,04%. Sedangkan hasil penetapan kadar sari ekstrak daun lidah mertua yang larutdalam air ialah 38,76% dan yang larut dalam etanol ialah 12,53%. Hasil fitokimia diketahui ekstrak daun lidah mertua mengandung saponin, flavonoid, steroid, dan triterpenoid, yangberfungsi dapat menghambat C. albicans.Kata kunci : daun lidah mertua (Sansevieria trifasciata Prain), Candida albicans, efektivitas,antikhamir
UJI EFEKTIVITAS EKSTRAK ETANOL BUAH PARE (Momordica charantia L.) SEBAGAI ANTIBAKTERI Salmonella typhi Oom Komala; Bina Lohita Sari; Nina Sakinah
FITOFARMAKA: Jurnal Ilmiah Farmasi Vol 2, No 1 (2012): FITOFARMAKA
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (209.257 KB) | DOI: 10.33751/jf.v2i1.165

Abstract

Penelitian yang bertujuan untuk mengetahui efektivitas antibakteri dari beberapa konsentrasi ekstrak etanol buah pare terhadap bakteri Salmonella typhi telah dilakukan. Pengujian antibakteri ekstrak etanol buah pare dilakukan dengan mengukur Diameter Daerah Hambat (DDH) melalui metode difusi kertas cakram dan Konsentrasi Hambat Minimum (KHM) melalui metode dilusi agar padat. Pengujian DDH dilakukan terhadap konsentrasi ekstrak buah pare 12,5%, 25%, 50%, 75%, serta kloramfenikol 30 UI sebagai kontrol positif dan aquadest sebagai kontrol negatif. Sedangkan pengujian KHM dilakukan terhadap konsentrasi 10%, 20%, 30%, 40%, 50%, 60%, 70% dan 80%. Hasil penelitian menunjukkan bahwa ekstrak etanol buah pare kurang efektif sebagai antibakteri terhadap Salmonella typhi, karena pada pengujian DDH zona hambat yang terbentuk tidak absolut. KHM berada pada konsentrasi 60%, dimana tidak ada pertumbuhan bakteri.Kata kunci: Buah pare, Salmonella typhi, efektivitas antibakteri
UJI AKTIVITAS ANTIBAKTERI SABUN WAJAH EKSTRAK DAUN BELUNTAS (Pluchea indica L.) TERHADAP Propionibacterium acnes Oom Komala; Septia Andini; Fatimah Zahra
FITOFARMAKA: Jurnal Ilmiah Farmasi Vol 10, No 1 (2020): FITOFARMAKA | Jurnal Ilmiah Farmasi
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/jf.v10i1.1717

Abstract

Propionibacterium acnes merupakan bakteri gram-positif berbentuk batang dan merupakan flora normal kulit yang ikut berperan dalam pembentukan jerawat. Sabun wajah lebih sering digunakan sebagai alternatif antijerawat karena telah dikenal masyarakat luas dan lebih praktis penggunaannya dan ekonomis. Penelitian ini bertujuan untuk mengkaji dan mengetahui sabun pembersih wajah antijerawat yang mengandung ekstrak daun beluntas yang paling efektif dalam menghambat pertumbuhan bakteri P. acne. Pada penelitian ini dilakukan penentuan nilai Konsentrasi Hambat Minimum (KHM) ekstrak daun beluntas terhadap bakteri P. acne selanjutnya dan formulasi sediaan sabun wajah ekstrak daun beluntas. Sediaan sabun wajah ini dilakukan uji mutu dan uji Lebar Daya Hambat (LDH). Hasil penelitian menunjukkan bahwa KHM ekstrak daun beluntas terdapat pada konsentrasi 5%. Sediaan sabun wajah ekstrak daun beluntas memiliki aktivitas antibakteri terhadap P. acne danyang paling efektif menghambat bakteri P. acne adalah formula 3 dengan konsentrasi 15% dengan rata-rata LDH sebesar 13,5 mm. Hasil dari uji hedonik menunjukan formula 1 lebih unggul dibandingkan dengan formula lainnya.
UJI ANTIBAKTERI SEDIAAN OBAT KUMUR EKSTRAK DAUN PANDAN WANGI (Pandanus amaryllifolius Roxb) TERHADAP Streptococcus mutans Oom Komala; Putri; Dwi Indriati
EKOLOGIA Vol 17, No 1 (2017): JURNAL ILMIAH ILMU DASAR DAN LINGKUNGAN HIDUP
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (211.407 KB) | DOI: 10.33751/ekol.v17i1.832

Abstract

Pandanus amaryllifolius Roxb leaf has potential as an antibacterial. The research aims to know the ability of stocks to mouthwash scented pandan leaf extract inhibits Streptococcus mutans and in determining the fragrant pandanus phytochemicals compounds. Wide-Area Drag testing (LDH) mouthwash extracts was performed with a concentration of 15%, 17.5% and 20%. Test the phytochemical saponins, alkaloids, tannins and flavonoids qualitatively on 0.5 g powder. Testing stabilita include organoleptik observations of color, aroma, taste preparation, test the pH, specific gravity and viscosity. The results showed that the material of pandanus leaf extract mouthwash scented antibacterial nature against Streptococcus mutans with a concentration of extracts of 15%, 17.5% and 20% showed values of LDH consecutive 16.3 mm; 22.6 mm and 28.3 mm earlier in the week while in week 8 loss to the value of LDH is 12.5 mm; 15.5 mm; and 19.6 mm. The active compounds in the leaves of pandanus is alkaloids, saponins, tannins and flavonoids. Mouthwashes stored at 25-30 C for 8 weeks are relatively stable for color, flavor, taste, pH, specific gravity, and viscosity, compared to a temperature of 40oC.Key words: Pandan fragrance, Streptococcus mutans, mouthwash
AKTIVITAS DEGRADASI SAMPAH ORGANIK DALAM BIOPORI Sri Wiedarti; M. Akhmar Yusfi Lubis; Oom Komala
EKOLOGIA Vol 15, No 1 (2015): JURNAL ILMIAH ILMU DASAR DAN LINGKUNGAN HIDUP
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (164.111 KB) | DOI: 10.33751/ekol.v15i1.204

Abstract

Biopori is pore space or in the ground formed by living creatures, such as microorganisms of the soil and plant roots. The shape resembles biopori hole in the ground and forked branches and very effective to channel water and air into the soil. In order to make the hole biopori still function optimally it should be routine coupled with organic material, so in the hole biopori absorption will still take place in aerobic composting process by soil microorganisms.The addition of microbes in accelerating the process of degradation of the garbage that is by adding a inoculum composite comprising four microorganism Saccharomyces, Lactobacillus, Acetobacter sp., and Bacillus. The addition of the best treatment with the addition of inoculum and soil, have physical characteristics that resemble the physical characteristics of the soil.Key words: Biopori, organic waste, microbes
POTENSI EKSTRAK CODIUM, HALIMEDA, DICTYOTA, CHONDRUS, GLACILLARIA SEBAGAI SUMBER PIGMEN DAN ANTIOKSIDAN ALAMI Tri Saptari Haryani; Uswatun Hasanah; Oom Komala; Intan Lestari; Dina Agustina
EKOLOGIA Vol 22, No 1 (2022): EKOLOGIA : JURNAL ILMIAH ILMU DASAR DAN LINGKUNGAN HIDUP
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/ekologia.v22i1.4739

Abstract

Macroalgae is one of the marine biological resources that has an important role in terms of economy, as a source of food and medicine that is beneficial to health. This study aims to determine the source of natural pigments and antioxidants extracts of algae Codium, Halimeda, Dictyota, Chondrus, and Glacillaria using acetone, Folin Ciocalteu, and 1,1-Diphenyl-2-Picrylhydrazil (DPPH) reagents. The method includes sampling, measuring water content by gravimetry, making extracts using maceration method, qualitative phytochemical testing, pigment source test for chlorophyll a, chlorophyll b, total chlorophyll, total phenol, and antioxidant activity. Phytochemical test results obtained that all samples contained flavonoid compounds, alkaloids, while Halimeda and Gracillaria extracts did not have terpenoids. The results of the test for the pigment source were the highest value obtained in Chondrus extract, which was 10.0038 mg/g. And from the results of antioxidant activity testing, all sample extracts were obtained including the group with moderate strength (IC50 value) ranging from 100-500).
Co-Authors . Ismanto . Yulianita, . Andhika Dhiasmi Anna Pradiningsih Asmara, Herra Indra Bina Lohita Sari Darda, Rouland Ibnu Dewi Sugiharti Dewi, Elfrieda Kusuma Dhiasmi, Andhika Dina Agustina Durrotun Nafisah Abd.Nf Dwi Indriati E Mulyati Effendi E. Mulyati Effendi Ella Noorlaela Faramitha, Ikhfina Dhea Fatah, Muhammad Fajar Saeful Fitria Dewi Sulistiyono Fuji Raka Siwi Hermawati, Ema Herra Indra Asmara Hery Widijanto Ike Yulia Ike Yulia Wiendarlina Indriati, Dwi Intan Lestari Joshie Ramadhan Lia Puspita Sari Lubis, M. Akhmar Yusfi M. Akhmar Yusfi Lubis Mahyuni, Siti Moerfiah Moerfiah Muhammad Alan Maulana Muztabadihardja Muztabadihardja Muztabadihardja Muztabadihardja Muztabadihardja, Muztabadihardja Muztabadihardja, Muztabadihardja Nina Sakinah Nina Sakinah Noorlaela, Ella Novi Fajar Utami Nurul Fadilah, Nurul Nur’aini, Putri Pebrianti, Rita Prasetyorini Prasetyorini Putri Putri Dwi Antini Putri Nur’aini Rahminiwati, Min Reni Rosyanti Reni Rosyanti Rikkit Rita Pebrianti Rita Rahmawati Rosyanti, Reni Rosyanti, Reni Salsabila, Nadia Muna Saraswati Darmawan Septia Andini Siti Mariyam Rosdiana Sri Suci Damayanti Sri Wardatun Sri Wiedarti Sri Wiedarti Sri Wiedarti Srinarayani, Kadek Sugiharti, Dewi Syamsidar, . Tri Retno Handayani, Marybet Tri Saptari Haryani Triastinurmiatiningsih, . Uswatun Hasanah Vira Mourena Putri Widjaya Yulia, Ike Yulianita Yulianita .