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Oom Komala
Universitas Pakuan
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UJI AKTIVITAS EKSTRAK Padina australis SEBAGAI ANTIBAKTERI Propionibacterium acnes PENYEBAB JERAWAT Vira Mourena Putri Widjaya; Oom Komala; . Ismanto
Ekologia: Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup Vol 21, No 1 (2021): Ekologia : Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/ekologia.v21i1.3147

Abstract

The apperance of acne is a problem for many suffers because can lower confidience and shame. Many people have attempted to treat or eliminate acne, that is by antibiotic. However of antibiotic clearly has side effects, and the ultilization of natural material is the solution. One of them is Padina australis which contains flavonoid, tanin dan saponin potentially as an antibacterial. The purpose of this research is to derermne the concentration of Padina australis extract with methanol 98% which has optimal activity in improving the growth of Propionibacterium acnes based on inhibition zone test and minimum inhibitory concetration (MIC). Qualitative phytochemical test were carried out on alkoloids, flavonoids, saponins, tannin and steroids. Testing these bacteria using disc dffusion methods. MIC of Padina australis extrct used dilution method with concentrtaion of 3.75%, 7.5%, 15% and 30%. Inhibition zone test used disc diffusion method with concentrtaion of 60%, 80% and 100%. The result of phytochemical screening testing Padina australis extrct test showed that there are secondary metabolite compounds alkoloids, flavonoids, saponins, tannin and steroids. MIC test of Padina australis extrct against Propionibacterium acnes occured at a concentration of 30% and the inhibition zone test was found at concentration of 100% which was the most optimal in inhibiting with an average of the inhibition zone width 6.20 mm.
UJI AKTIVITAS EKSTRAK ETANOL KOMBINASI DAUN PANDAN WANGI DAN DAUN JAMBU BIJI TERHADAP Shigella dysenteriae Oom Komala; Durrotun Nafisah Abd.Nf; Novi Fajar Utami
Ekologia: Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup Vol 21, No 2 (2021): Ekologia : Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/ekologia.v21i2.3666

Abstract

Dysentery can be treated using Pandanus amarylifolius leaves and Psidium guajava leaves. This study aimed to determine the activity of the combination of 96% ethanol extract of P. amarylifolius leaves and P. guajava leaves in inhibiting the growth of Shigella dysenteriae bacteria. Methods. Extraction of P. amarylifolius leaves and P. guajava leaves respectively by using the maceration method with 96% ethanol solvent. For the combination treatment, each of these extracts was combined. Each extract obtained was tested for minimum inhibitory concentration (MIC). The MIC value of P. amarylifolius leaf extract was obtained at a concentration of 30% and P. guajava leaf extract was obtained at a concentration of 40%. Inhibitory width test (IWT) by disc diffusion method was carried out on single extracts and combination extracts, with treatment concentrations of 30% single extract of P. amarylifolius leaf (K1), 40% single extract of P. guajava leaf (K2), combination 1 (P. amarylifolius leaf). 30%+ P. guajava leaf 40%) (K3), combination 2 (35% P. amarylifolius leaf + 40% P. guajava leaf) (K4), combination 3 (40% P. amarylifolius leaf + 40% P. guajava leaf) (K5). The width of the inhibition of the research results of the treatment against S. dysenteriae were analyzed using a completely randomized design and Duncan's test. The results showed that the combination of P. amarylifolius leaf extract and P. guajava leaf was more effective in inhibiting the growth of S. dysenteriae bacteria. The combination of P. amarylifolius leaf extract and P. guajava leaf extract that was most effective in inhibiting the growth of S. dysenteriae bacteria was combination 3 (40% P. amarylifolius leaf extract + 40% P. guajava leaf extract) with an average inhibitory width of 5.625 mm. Keywords: Combination, P. amarylifolius Leaves, P.guajava Leaves, S. dysenteriae
AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL BIJI KAPULAGA JAWA (Amomum compactum Soland. ex Maton) TERHADAP Streptococcus pyogenes Oom Komala; . Ismanto; Muhammad Alan Maulana
Ekologia: Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup Vol 20, No 1 (2020): Ekologia : Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/ekologia.v20i1.1985

Abstract

Streptococcus pyogenes is one of the pathogenic bacteria that causes pharyngitis. Natural treatment to overcome these problems is to use cardamom seeds. The purpose of this study was to test the antibacterial activity, determine the concentration of inhibitory zone and phytochemical compounds from  ethanol 96% extract of Java cardamom seeds (Amomum compactum Soland. Ex Maton) against Streptococcus pyogenes. The method is used   solid dilution and paper disc diffusion method. The solid dilution method is used for the Minimum Inhibitory Concentration (MIC) test with a concentration of 1.25%, 2.5%, 5% and 7.5% while the paper diffusion method is used for the Inhibition zone Diameter (IZD)  using five treatments namely three concentrations of ethanol 96% extract of Java cardamom seeds (7.5%, 10% and 12%), one positive control of amoxicillin 0.01 mg/mL and one negative control of sterile distilled water. IZD data were  analyzed using ANOVA with a confidence level of 95% and α = 0.05 and Duncan's further tests to determine differences between treatments. The results showed that the MIC  was at a concentration of 7.5% while for the IZD test which had the highest activity there was a concentration of 12% with an average inhibition diameter of 12.03 ± 0.14 mm. In addition, ethanol 96% extract of Java cardamom seeds contain alkaloids, flavonoids, terpenoids and tannins which function in antibacterial activity.
PERBANDINGAN DAYA HAMBAT DARI EKSTRAK DAN HASIL FERMENTASI BAWANG PUTIH (Allium sativum) TERHADAP BAKTERI Escherichia coli Oom Komala; Putri Dwi Antini; Novi Fajar Utami
Ekologia: Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup Vol 22, No 2 (2022): Ekologia : Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/ekologia.v22i2.6259

Abstract

Allium sativum is a plant that generally grows in the highlands and is used to treat digestion, anti-inflammatory, hypertension. Meanwhile, fermented onions are fermented garlic for 40 days and are used as medicine for diabetes, hypertension, hypercholesterolemia. These fermented garlic and fresh garlic contain alkaloids, flavonoids, saponins, and tannins. The purpose of this study was to compare the antibacterial activity of fermented garlic and garlic by determining the minimum inhibitory concentration (MIC) and Inhibitory Width (LDH) against E. coli bacteria. Method: Garlic fermentation is done by wrapping fresh garlic in aluminum foil and putting it in a rice cooker for 40 days. MIC test at concentrations of 20%, 25%, 50% and 75% with agar dilution method and LDH test with disc diffusion method at concentrations of 25%, 50% and 75%. The results showed that fermented garlic and garlic extracts were able to inhibit E. coli bacteria with the Minimum Inhibitory Concentration (MIC) at a concentration of 25%. Garlic extract is the most optimal extract at a concentration of 75% having antibacterial activity compared to fermented onion extract with an average Inhibitory Width (LDH) of 7.43 mm in the medium category. Conclusion: fresh garlic extract had better antibacterial activity than fermented garlic extract against E. coli.
PENGELOLAAN SAMPAH ORGANIK MENGGUNAKAN MIKROORGANISME Oom Komala; Dewi Sugiharti; Rouland Ibnu Darda
Ekologia: Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup Vol 12, No 2 (2012): Ekologia : Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/ekol.v12i2.239

Abstract

The study of organic waste using microorganisms has been carried out, aiming to find the concentration of microorganisms that can deodorize and can degrade organic waste into compost and fertilize the plants. The method used for waste is experimental with 4 treatment is not given microbe, 25 ml microbe concentrations of 106, 50 ml microbe concentrations of 106 and 75 ml microbe concentration of 106 under anaerobic conditions. After 20 days of fermenting compost is given at 1 month age chilli crop at a dose of 15 g per week for 5 weeks. Waste parameter includes aroma, pH, texture and temperature. In chilli crop plant height, number of leaves, fresh weight and dry weight, were analyzed using factorial completely randomized design 4x4 to see the influence of  crops. The results can be seen that organic waste can be managed using a mix of microbes into compost that can improve plant growth chillies with a dose of 25 ml concentrations of 106 of the best.
Meningkatkan Motivasi Bisnis Melalui Pembuatan Jamu Temulawak (Curcuma Zanthorrhiza) Dalam Bentuk Sediaan Minuman Kesehatan Prasetyorini Prasetyorini; Oom Komala; E Mulyati Effendi; Moerfiah Moerfiah
KENDURI : Jurnal Pengabdian dan Pemberdayaan Masyarakat Vol. 2 No. 3 (2022): Desember
Publisher : Yayasan Darussalam Bengkulu

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Abstract

Salah satu kebijakan pembangunan Desa Cibadak, Kecamatan Ciampea, Kabupaten Bogor yang masih menemui kendala adalah bidang ekonomi tepatnya pada aspek industri. Antara lain jumlah usaha ekonomi produktif yang masih sedikit. Hal ini disebabkan masih kurangnya kemampuan warga dalam menghasilkan produk rumah tangga yang bisa menjadi produk unggulan. Temulawak adalah tanaman asli Indonesia. Rimpang temulawak mengandung kurkuminoid, minyak atsiri, pati, protein, lemak, selulosa dan mineral, serta sudah dikenal banyak khasiatnya. Umumnya Ibu Rumah Tangga Desa Cibadak sudah mengenal temulawak. Tujuan kegiatan pengabdian pada masyarakat adalah memberikan penyuluhan dan pendampingan pembuatan jamu seduhan dan serbuk, serta memberi motivasi untuk penanaman temulawak bagi Kelompok Pemberdayaan Ibu Rumah Tangga Desa Cibadak, kecamatan Ciampea Kabupaten Bogor. Metode pelaksanaan meliputi pelatihan, pembuatan, pengemasan sediaan jamu seduhan dan serbuk temulawak. Hasil iptek untuk kegiatan pengabdian kepada masyarakat ini disimpulkan bahwa pelatihan pembuatan jamu temulawak dapat meningkatkan motivasi untuk mengembangkan jamu temulawak 77,8%, meningkatakan motivasi untuk menjual produk temulawak 88,9%, baik jamu seduhan segar (44,4%) maupun serbuk (55,6%).
Peningkatan Bisnis Melalui Pelatihan Pembuatan Sediaan Bumbu Dapur Dalam Bentuk Serbuk Prasetyorini Prasetyorini; Oom Komala; E Mulyati Effendi; Moerfiah Moerfiah
KENDURI : Jurnal Pengabdian dan Pemberdayaan Masyarakat Vol. 2 No. 3 (2022): Desember
Publisher : Yayasan Darussalam Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Desa Cibadak adalah sebuah desa mitra binaan yang terletak di Kecamatan Ciampea Kabupaten Bogor. Salah satu kebijakan pembangunan Desa Cibadak yang masih menemui kendala adalah di bidang ekonomi tepatnya pada aspek industri. Antara lain jumlah usaha ekonomi produktif yang masih sedikit. Hal ini disebabkan masih kurangnya kemampuan warga dalam menghasilkan produk rumah tangga yang bisa menjadi produk unggulan. Tujuan kegiatan pengabdian adalah meningkatnya pengetahuan dan keterampilan ibu-ibu rumah tangga yang tergabung pada Kelompok Pemberdayaan Ibu Rumah Tangga Desa Cibadak melalui pendampingan pembuatan bumbu dapur dalam bentuk serbuk sehingga bisa membuat produk industri rumah tangga yang diharapkan dapat meningkatan perekonomian keluarga. Metode pelaksanaan meliputi pelatihan, pembuatan, pengemasan sediaan serbuk aneka bumbu dapur (jahe, sereh, bawang putih, ketumbar, merica, dan kunyit). Hasil iptek untuk kegiatan pengabdian kepada masyarakat ini disimpulkan bahwa pelatihan pembuatan serbuk bumbu dapur pada ibu-ibu kelompok masyarakat industri rumah tangga Perum Dramaga Pratama RT06, Desa Cibadak kecamatan Ciampea Kabupaten Bogor dapat meningkatkan pengetahuan ibu-ibu rumah tangga 80%. Dari hasil evaluasi diketahui bahwa pengetahuan membuat serbuk bumbu dapur ini sudah dimulai menjadi bisnis 50%
UJI AKTIVITAS EKSTRAK ETANOL DAUN TAPAK DARA (Catharanthus roseus L.) SEBAGAI ANTIBAKTERI Streptococcus mutans Triastinurmiatiningsih, .; Komala, Oom; Salsabila, Nadia Muna
Ekologia: Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup Vol 23, No 2 (2023): Ekologia : Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/ekologia.v23i2.9605

Abstract

Dental caries is a localized dental disease that damages the hard tissues of the teeth formed from the accumulation of plaque on the tooth surface.  Tapak dara leaf (Catharanthus roseus L.) is known to have antibacterial activity derived from secondary metabolites, namely Triterpenoid Indole Alkaloid (TIA), flavonoids, saponins, steroids, and tannins. The purpose of the study was to analyze the activity of 96% ethanol extract of tapak dara leaves with the highest inhibitory power against Streptococcus mutans bacteria and determine chemical compounds through phytochemical tests. Antibacterial testing of 96% ethanol extract of virgin tread leaves using disc diffusion method with LDH (Width Inhibitory Power) measurement. Antibacterial tests of ethanol extract of tapak dara leaf were carried out on concentrations of 60%, 65%, 70%, and 75%. DMSO solvent 10% as a negative control and amoxicillin antibiotic 500 ppm as a positive control. The results showed that antibacterial test results at a concentration of 75% showed antibacterial activity with the highest inhibitory power averaging 6.27 mm. Phytochemical test results show that 96% ethanol extract of tapak dara leaf contains alkaloids, flavonoids, tannins, saponins, and steroids
UJI AKTIVITAS TITER ANTIBODI AYAM PETELUR DAN PASCA VAKSINASI NEWCASTLE DISEASE INAKTIF Faramitha, Ikhfina Dhea; Komala, Oom; Syamsidar, .
Ekologia: Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup Vol 24, No 1 (2024): Ekologia : Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/ekologia.v24i1.9634

Abstract

Laying hens in Indonesia are outstanding and potential commodities. However, Newcastle Disease (ND) is one of the risks that must be faced. Now vaccination is chosen as one of the strategies to prevent ND. This paper aims to examine the effect of inactivated ND vaccine from Lentogenic and Velogenic strains on the increase in antibody titers of laying hens. To find the antibody titer value, the Hemagglutination Inhibition (HI) test method is used with serum sampling time before vaccination, 4th week, 6th week, 8th week, and 10th week post-vaccination. Meanwhile, to see the significance of the difference in titers at each time, a statistical analysis was carried out using the Paired Sample T-test. From this analysis, it is known that the Lentogenic strain has the highest significance at week 8 post-vaccination with a value of 1779.20 compared to 380.80 before vaccination. Meanwhile, the Velogenic strain had the highest significance at week 4 post-vaccination with a value of 1753.60 compared to 143.60 before vaccination. So it can be concluded that the Inactivated ND vaccine is effective in stimulating the formation of protective antibodies to fight against the ND virus attack.
Effectiveness of Citronella Extract (Cymbopogon nardus) Mouthwash From Microwave-Assisted Extraction Method Against Streptococcus mutans Komala, Oom; Mahyuni, Siti; Pradiningsih, Anna; Dewi, Elfrieda Kusuma
FITOFARMAKA: JURNAL ILMIAH FARMASI Vol 14, No 1 (2024): FITOFARMAKA: Jurnal Ilmiah Farmasi
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/jf.v14i1.9621

Abstract

Cymbopogon nardus, or citronella has a distinctive aroma is known as a source of essential oils. Extract of citronella can be use as mouthwash to reduce bad breath and protect teeth from bacteria that cause dental caries. C. nardus contains antibacterial compounds such as flavonoid, essential oils, polyphenols, and saponin which are also act as antioxidant compounds. This research was carried out to formulate mouthwash using citronella extract and determine the antibacterial activity of the citronella mouthwash against S. mutans bacteria using disc diffusion method. The microwave  extraction method (MAE) was used to obtain extract using  70 % and  96 % ethanol as  a solvents. The mouthwash were made in 3 formulas i.e.  formula 1, formula 2, and formula 3 each contain 25 % w/v, 30 % w/v, and 35 % w/v) respectively. The quality of mouthwash includes organoleptic, pH, viscosity and hedonic was evaluated and antibacterial activity was determined by measuring the value of minimum inhibitory concentration (MIC) and diameter of inhibition zone. The MIC value of citronella extract was achieved at concentration 20 %. All mouthwash formula met the requirements for a good mouthwash. Formula 1 was the most preferred formula by the panelists based on the hedonic test.  Formula 3 had the strongest antibacterial activity with diameter inhibitory of 17.6 ± 0.52 mm. It was concluded that citronella mothwash has the potential to be developed into an antibacterial mouthwash.
Co-Authors . Ismanto . Yulianita, . Andhika Dhiasmi Anna Pradiningsih Asmara, Herra Indra Bina Lohita Sari Darda, Rouland Ibnu Dewi Sugiharti Dewi, Elfrieda Kusuma Dhiasmi, Andhika Dina Agustina Durrotun Nafisah Abd.Nf Dwi Indriati E Mulyati Effendi E. Mulyati Effendi Ella Noorlaela Faramitha, Ikhfina Dhea Fatah, Muhammad Fajar Saeful Fitria Dewi Sulistiyono Fuji Raka Siwi Hermawati, Ema Herra Indra Asmara Hery Widijanto Ike Yulia Ike Yulia Wiendarlina Indriati, Dwi Intan Lestari Joshie Ramadhan Lia Puspita Sari Lubis, M. Akhmar Yusfi M. Akhmar Yusfi Lubis Mahyuni, Siti Moerfiah Moerfiah Muhammad Alan Maulana Muztabadihardja Muztabadihardja Muztabadihardja Muztabadihardja Muztabadihardja, Muztabadihardja Muztabadihardja, Muztabadihardja Nina Sakinah Nina Sakinah Noorlaela, Ella Novi Fajar Utami Nurul Fadilah, Nurul Nur’aini, Putri Pebrianti, Rita Prasetyorini Prasetyorini Putri Putri Dwi Antini Putri Nur’aini Rahminiwati, Min Reni Rosyanti Reni Rosyanti Rikkit Rita Pebrianti Rita Rahmawati Rosyanti, Reni Rosyanti, Reni Salsabila, Nadia Muna Saraswati Darmawan Septia Andini Siti Mariyam Rosdiana Sri Suci Damayanti Sri Wardatun Sri Wiedarti Sri Wiedarti Sri Wiedarti Srinarayani, Kadek Sugiharti, Dewi Syamsidar, . Tri Retno Handayani, Marybet Tri Saptari Haryani Triastinurmiatiningsih, . Uswatun Hasanah Vira Mourena Putri Widjaya Yulia, Ike Yulianita Yulianita .