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Oom Komala
Universitas Pakuan
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IDENTIFIKASI BAKTERI DARI PUPUK ORGANIK CAIR ISI RUMEN SAPI Sri Suci Damayanti; Oom Komala; E. Mulyati Effendi
EKOLOGIA Vol 18, No 2 (2018): EKOLOGIA: JURNAL ILMIAH ILMU DASAR DAN LINGKUNGAN HIDUP
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (336.312 KB) | DOI: 10.33751/ekol.v18i2.1627

Abstract

Waste treatment technology is not less important for the business of Slaughterhouse (RPH). Various businesses and technologies have been done to handle this waste problem, especially for the rumen waste in RPH. One of them is utilizing the waste as an organic fertilizer. Bovine rumen bacteria are bacteria that originate from the contents of bovine rumen, with simple and inexpensive development techniques. This bacterium helps farmers in improving and developing an insightful agriculture on the preservation of soil fertility and natural resources. The study aims to identify bacteria and find out the diversity, properties, and characteristics of bacteria from the liquid organic content of rumen cows. Methods undertaken by observing colony morphology, colony selection and purification, Gram staining, spores staining, and biochemical testing. Of the four isolates identified there are two bacterial genus, three isolates RS1a, RS2a, RS2b including the genus Bacillus sp. aerobic and an isolates of RS1b including the genus Lactobacillus sp. the facultative of anaerobic. The four isolates of bacteria are able to grow optimum at pH 6.5 and the temperature of 370C is homofermentative.
UJI EFEKTIVITAS ANTIBAKTERI PERASAN SEGAR DAN SERBUK UMBI BAWANG PUTIH (Allium sativum L.) TERHADAP BAKTERI Salmonella typhi Oom Komala; Herra Indra Asmara; Ike Yulia Wiendarlina
EKOLOGIA Vol 14, No 2 (2014): JURNAL ILMIAH ILMU DASAR DAN LINGKUNGAN HIDUP
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (159.172 KB) | DOI: 10.33751/ekol.v14i2.216

Abstract

Herbs and spices are very important and useful as therapeutic agent against typhoid fever. Increasing multidrug resistance of pathogens forces to find alternative compounds for treatment of these infectious diseases. Garlic contained volatile oil and alisin which containing sulfur. The present study was to evaluate the antibacterial potential of fresh and powder of garlic (Allium sativum L.) extracts against the bacterial pathogens Salmonella typhi and describes the phytochemical analysis. Methods: In the present study the antimicrobial potency of fresh and powder of garlic was carried out by disc diffusion and dilution method against Salmonella typhi bacterial and qualitatively tested for the presence of chemical constituents for the identification of flavonoids, saponins, tannins, alkaloids by the method described previously. The results of the present study showed the fresh and powder of garlic (Allium sativum L.) same as susceptible for Salmonella typhi. The (minimum inhibitory concentration) MIC of fresh of garlic at 10% and the powder of garlic at 8%. The most effective concentration of fresh and powder of garlic for Salmonella typhi is 90 %. Phytochemical screening of ethanol extract of Allium sativum L revealed the presence of various bioactive components of flavonoids, saponins, tannins and alkaloids were the most prominent. Key words : Allium sativum, Antibacterial, Salmonella typhi
UJI ANTIBAKTERI DAN FORMULASI SEDIAAN MASKER ANTI JERAWAT YANG MENGANDUNG KAYU MANIS (Cinnamomum burmanni Nees &T. Nees ) Oom Komala; Ella Noorlaela; Andhika Dhiasmi
EKOLOGIA Vol 18, No 1 (2018): EKOLOGIA: JURNAL ILMIAH ILMU DASAR DAN LINGKUNGAN HIDUP
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (536.495 KB) | DOI: 10.33751/ekol.v18i1.836

Abstract

Cinnamon contains eugenol and cinnamaldehyde compounds and is widely used as an anti acne. The aim of this research make cinnamon maskers which has antibacterial power of Staphylococus aureus, to know the effect of cinnamon maskers storage to quality, and know Cinnamomum burmanii maskers prefered by panelist. Cinnamon extract is made by maceration method, Test the antibacterial activity of maskers by diffusion method. The evaluation of maskers is done stability test at room temperature (25oC-30oC) and temperature 40oC for 8 weeks, and test prefered by panelists. Cinnamon extract maskers is made with various concentrations of formula 1 with cinnamon extract 0%, formula 2 with 8% concentration, formula 3 with 10% concentration and formula 4 with 12% concentration, and tetracycline is control. The results of the study were known maskers with cinnamon 12% concentration most effective as an antibacterial with average of 16 mm diameter and most preferred, stable at room temperature 25oC-30oC and 40oC for 8 weeks. pH of Maskers ranges from 5.31 to 6.89 which is still the normal range for pH of the preparation.Key words: Pandan fragrance, Streptococcus mutans, mouthwash
UJI AKTIVITAS EKSTRAK Padina australis SEBAGAI ANTIBAKTERI Propionibacterium acnes PENYEBAB JERAWAT Vira Mourena Putri Widjaya; Oom Komala; . Ismanto
EKOLOGIA Vol 21, No 1 (2021): EKOLOGIA : JURNAL ILMIAH ILMU DASAR DAN LINGKUNGAN HIDUP
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/ekologia.v21i1.3147

Abstract

The apperance of acne is a problem for many suffers because can lower confidience and shame. Many people have attempted to treat or eliminate acne, that is by antibiotic. However of antibiotic clearly has side effects, and the ultilization of natural material is the solution. One of them is Padina australis which contains flavonoid, tanin dan saponin potentially as an antibacterial. The purpose of this research is to derermne the concentration of Padina australis extract with methanol 98% which has optimal activity in improving the growth of Propionibacterium acnes based on inhibition zone test and minimum inhibitory concetration (MIC). Qualitative phytochemical test were carried out on alkoloids, flavonoids, saponins, tannin and steroids. Testing these bacteria using disc dffusion methods. MIC of Padina australis extrct used dilution method with concentrtaion of 3.75%, 7.5%, 15% and 30%. Inhibition zone test used disc diffusion method with concentrtaion of 60%, 80% and 100%. The result of phytochemical screening testing Padina australis extrct test showed that there are secondary metabolite compounds alkoloids, flavonoids, saponins, tannin and steroids. MIC test of Padina australis extrct against Propionibacterium acnes occured at a concentration of 30% and the inhibition zone test was found at concentration of 100% which was the most optimal in inhibiting with an average of the inhibition zone width 6.20 mm.
UJI AKTIVITAS EKSTRAK ETANOL KOMBINASI DAUN PANDAN WANGI DAN DAUN JAMBU BIJI TERHADAP Shigella dysenteriae Oom Komala; Durrotun Nafisah Abd.Nf; Novi Fajar Utami
EKOLOGIA Vol 21, No 2 (2021): EKOLOGIA : Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/ekologia.v21i2.3666

Abstract

Dysentery can be treated using Pandanus amarylifolius leaves and Psidium guajava leaves. This study aimed to determine the activity of the combination of 96% ethanol extract of P. amarylifolius leaves and P. guajava leaves in inhibiting the growth of Shigella dysenteriae bacteria. Methods. Extraction of P. amarylifolius leaves and P. guajava leaves respectively by using the maceration method with 96% ethanol solvent. For the combination treatment, each of these extracts was combined. Each extract obtained was tested for minimum inhibitory concentration (MIC). The MIC value of P. amarylifolius leaf extract was obtained at a concentration of 30% and P. guajava leaf extract was obtained at a concentration of 40%. Inhibitory width test (IWT) by disc diffusion method was carried out on single extracts and combination extracts, with treatment concentrations of 30% single extract of P. amarylifolius leaf (K1), 40% single extract of P. guajava leaf (K2), combination 1 (P. amarylifolius leaf). 30%+ P. guajava leaf 40%) (K3), combination 2 (35% P. amarylifolius leaf + 40% P. guajava leaf) (K4), combination 3 (40% P. amarylifolius leaf + 40% P. guajava leaf) (K5). The width of the inhibition of the research results of the treatment against S. dysenteriae were analyzed using a completely randomized design and Duncan's test. The results showed that the combination of P. amarylifolius leaf extract and P. guajava leaf was more effective in inhibiting the growth of S. dysenteriae bacteria. The combination of P. amarylifolius leaf extract and P. guajava leaf extract that was most effective in inhibiting the growth of S. dysenteriae bacteria was combination 3 (40% P. amarylifolius leaf extract + 40% P. guajava leaf extract) with an average inhibitory width of 5.625 mm. Keywords: Combination, P. amarylifolius Leaves, P.guajava Leaves, S. dysenteriae
AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL BIJI KAPULAGA JAWA (Amomum compactum Soland. ex Maton) TERHADAP Streptococcus pyogenes Oom Komala; . Ismanto; Muhammad Alan Maulana
EKOLOGIA Vol 20, No 1 (2020): EKOLOGIA : JURNAL ILMIAH ILMU DASAR DAN LINGKUNGAN HIDUP
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (155.499 KB) | DOI: 10.33751/ekologia.v20i1.1985

Abstract

Streptococcus pyogenes is one of the pathogenic bacteria that causes pharyngitis. Natural treatment to overcome these problems is to use cardamom seeds. The purpose of this study was to test the antibacterial activity, determine the concentration of inhibitory zone and phytochemical compounds from  ethanol 96% extract of Java cardamom seeds (Amomum compactum Soland. Ex Maton) against Streptococcus pyogenes. The method is used   solid dilution and paper disc diffusion method. The solid dilution method is used for the Minimum Inhibitory Concentration (MIC) test with a concentration of 1.25%, 2.5%, 5% and 7.5% while the paper diffusion method is used for the Inhibition zone Diameter (IZD)  using five treatments namely three concentrations of ethanol 96% extract of Java cardamom seeds (7.5%, 10% and 12%), one positive control of amoxicillin 0.01 mg/mL and one negative control of sterile distilled water. IZD data were  analyzed using ANOVA with a confidence level of 95% and α = 0.05 and Duncan's further tests to determine differences between treatments. The results showed that the MIC  was at a concentration of 7.5% while for the IZD test which had the highest activity there was a concentration of 12% with an average inhibition diameter of 12.03 ± 0.14 mm. In addition, ethanol 96% extract of Java cardamom seeds contain alkaloids, flavonoids, terpenoids and tannins which function in antibacterial activity.
PENGELOLAAN SAMPAH ORGANIK MENGGUNAKAN MIKROORGANISME Oom Komala; Dewi Sugiharti; Rouland Ibnu Darda
EKOLOGIA Vol 12, No 2 (2012): JURNAL ILMIAH ILMU DASAR DAN LINGKUNGAN HIDUP
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (350.405 KB) | DOI: 10.33751/ekol.v12i2.239

Abstract

The study of organic waste using microorganisms has been carried out, aiming to find the concentration of microorganisms that can deodorize and can degrade organic waste into compost and fertilize the plants. The method used for waste is experimental with 4 treatment is not given microbe, 25 ml microbe concentrations of 106, 50 ml microbe concentrations of 106 and 75 ml microbe concentration of 106 under anaerobic conditions. After 20 days of fermenting compost is given at 1 month age chilli crop at a dose of 15 g per week for 5 weeks. Waste parameter includes aroma, pH, texture and temperature. In chilli crop plant height, number of leaves, fresh weight and dry weight, were analyzed using factorial completely randomized design 4x4 to see the influence of crops. The results can be seen that organic waste can be managed using a mix of microbes into compost that can improve plant growth chillies with a dose of 25 ml concentrations of 106 of the best.
AKTIVITAS ANTIJAMUR EKSTRAK ETANOL 50% DAN ETANOL 96% DAUN PACAR KUKU Lawsonia inermis L TERHADAP Trichophyton mentagrophytes Oom Komala; Yulianita .; Fuji Raka Siwi
EKOLOGIA Vol 19, No 1 (2019): EKOLOGIA: JURNAL ILMIAH ILMU DASAR DAN LINGKUNGAN HIDUP
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (279.672 KB) | DOI: 10.33751/ekol.v19i1.1657

Abstract

Trichopyton mentagrophytes is a type of fungus that belongs to a group of dermatophytes, and a disease that causes dermatophytosis (ringworm), this fungus likes parts that contain keratin substances such as skin, hair / hair, nails or horns. This study aims to determine the antifungal activity of 50% and 96% ethanol extract. KHM testing was made with a concentration of 2.5%, 5%, 7.5% and 10% with the dilution method to determine KHM. Whereas in testing the width of the inhibitory area (LDH) was carried out after getting the MIC value at concentrations of 10%, 25% and 50% with disc diffusion method to determine the effective LDH and qualitative phytochemical testing. The results showed that 96% ethanol solvent at a concentration of 50% could inhibit the fungus Trichopyton mentagrophytes compared to 50% ethanol as seen from the inhibition zone formed in the width of the inhibitory area. Secondary metabolite compounds found in 50% ethanol extract and 96% ethanol are flavonoids, alkaloids, saponins, tannins, quinones and terpenoids.
Effectiveness of Leaves Extract Fractions of Archidendron jiringa (Jack) I.C Nielsen Against Microbes Oom Komala; Sri Wardatun; Lia Puspita Sari
Molekul Vol 14, No 2 (2019)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (296.568 KB) | DOI: 10.20884/1.jm.2019.14.2.538

Abstract

Archidendron jiringa(Jack) I.C Nielsen) contains a lot of essential oils, saponins, alkaloids, terpenoids, steroids, tannins, glycosides, and flavonoids. Flavonoid is a secondary metabolite compound in a plant as antimicrobial. This study aims to determine the antimicrobial activity of fractions of ethanol 96%, n-hexan, ethyl acetate, and ethanol-water leaves extractand determine levels of favonoids.  A. jiringaleaves extract were tested against Streptococus mutans, Pseudomonas aeruginosa, and Candida albicans.  The research using agar disc diffusion technique for antimicrobial  activity and colorimetric method to know the total of flavonoid. Chloramphenicol and ketoconazole were used as a reference standard. The result of this study shows fractions of ethanol 96%,  n-hexane,  ethyl acetate, and  ethanol-water A. jiringaleaves extract  can inhibit microbial growth of S. mutans, P. aeruginosa, and C. albicans.Total of flavonoids on the extract respectively are 1.13%, 0.494%, 2.337%, and 0.549%. determined with complementary colorimetry to each leaf extract fractionA. jiringawith aluminum chloride method. Absorption spectrum measurement using a spectrophotometer.  The higher  percent of flavonoids would cause greater    bacteriainhibitory  zone but  only  0.090 for  pearson correlation value. Conclusions ethyl acetate leaves extract most effective inhibit microbial growth from the other fractions.
Pelatihan Pembuatan Yoghurt berbahan Dasar Susu Kedelai (Glycine soja) dalam Meningkatkan Motivasi Berwirausaha Oom Komala
Charity : Jurnal Pengabdian Masyarakat Vol 4 No 2 (2021): Charity-Jurnal Pengabdian Masyarakat
Publisher : PPM Universitas Telkom

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25124/charity.v4i2.2856

Abstract

Masalah mitra adalah kelompok ibu-ibu PKK dengan pendapatan rendah, tidak mengetahui pembuatan produk yogurt dan tidak mengetahui pemasaran produk. Dari masalah ini para mitra sepakat untuk belajar bagaimana membuat yogurt yang terbuat dari susu kedelai (Glycine soja) yang dapat diproduksi dengan nilai ekonomis dan membuat keluarga sehat.Tujuan dari kegiatan pelatihan dan pendampingan yogurt adalah bentuk kegiatan pelayanan masyarakat dan bermitra dengan ibu-ibu PKK di Desa Loji, Kecamatan Bogor Barat, Kota Bogor. Metode pelaksanaan yang dilakukan adalah 1. memberikan pelatihan tentang pembuatan yogurt yang terbuat dari susu kedelai, 2. pelatihan dan membantu dalam pembuatan kemasan dan pemasaran. Hasil dari kegiatan ini adalah meningkatkan pengetahuan, keterampilan mitra, dan produk yogurt berbasis susu kedelai berlabel.
Co-Authors . Ismanto . Yulianita, . Andhika Dhiasmi Anna Pradiningsih Asmara, Herra Indra Bina Lohita Sari Darda, Rouland Ibnu Dewi Sugiharti Dewi, Elfrieda Kusuma Dhiasmi, Andhika Dina Agustina Durrotun Nafisah Abd.Nf Dwi Indriati E Mulyati Effendi E. Mulyati Effendi Ella Noorlaela Faramitha, Ikhfina Dhea Fatah, Muhammad Fajar Saeful Fitria Dewi Sulistiyono Fuji Raka Siwi Hermawati, Ema Herra Indra Asmara Hery Widijanto Ike Yulia Ike Yulia Wiendarlina Indriati, Dwi Intan Lestari Joshie Ramadhan Lia Puspita Sari Lubis, M. Akhmar Yusfi M. Akhmar Yusfi Lubis Mahyuni, Siti Moerfiah Moerfiah Muhammad Alan Maulana Muztabadihardja Muztabadihardja Muztabadihardja Muztabadihardja Muztabadihardja, Muztabadihardja Muztabadihardja, Muztabadihardja Nina Sakinah Nina Sakinah Noorlaela, Ella Novi Fajar Utami Nurul Fadilah, Nurul Nur’aini, Putri Pebrianti, Rita Prasetyorini Prasetyorini Putri Putri Dwi Antini Putri Nur’aini Rahminiwati, Min Reni Rosyanti Reni Rosyanti Rikkit Rita Pebrianti Rita Rahmawati Rosyanti, Reni Rosyanti, Reni Salsabila, Nadia Muna Saraswati Darmawan Septia Andini Siti Mariyam Rosdiana Sri Suci Damayanti Sri Wardatun Sri Wiedarti Sri Wiedarti Sri Wiedarti Srinarayani, Kadek Sugiharti, Dewi Syamsidar, . Tri Retno Handayani, Marybet Tri Saptari Haryani Triastinurmiatiningsih, . Uswatun Hasanah Vira Mourena Putri Widjaya Yulia, Ike Yulianita Yulianita .