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All Journal e-Journal Pustaka Kesehatan STOMATOGNATIC- Jurnal Kedokteran Gigi ODONTO Dental Journal Insisiva Dental Journal : Majalah Kedokteran Gigi Insisiva Dental Journal (Majalah Kedokteran Gigi) Padjadjaran Journal of Dentistry Abdimas Universal Jurnal Karinov MDJ (Makassar Dental Journal) Majalah Biomorfologi (Biomorphology Journal) Denta: Jurnal Kedokteran Gigi Odonto dental journal Jurnal Kedokteran Meditek
Yani Corvianindya Rahayu
Departement Of Oral Biology Faculty Of Dentistry University Of Jember
Humanities Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Dentistry Economics, Econometrics & Finance Education Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Library & Information Science Medicine & Pharmacology Neuroscience Public Health Social Sciences

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THE CYTOTOXIC AND PROLIFERATIVE ACTIVITY OF COCOA POD HUSK EXTRACT (THEOBROMA CACAO L.) ON PERIODONTAL LIGAMENT FIBROBLASTS Rahayu, Yani Corvianindya; Triwahyuni, Iin Eliana; Kusumawardani, Banun; Sari, Dewi Yunita
Odonto : Dental Journal Vol 9, No 1 (2022): July 2022
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (394.479 KB) | DOI: 10.30659/odj.9.1.46-52

Abstract

Background: Theobroma cacao was considered agricultural wastes. Cocoa pod husk contain various bioactive compounds as natural antioxidants,antibacterial and anti-inflammatory. A good biomaterial must be non-toxic and have no detrimental effect on the biological environment. The aim of research was to determine the cytotoxicity and proliferative activity of cocoa pod husk extract on primary cells of periodontal ligament fibroblasts. Method: This experimental laboratory used primary cell culture of periodontal ligament fibroblasts. The cytotoxicity and proliferative activity were analyzed by MTT assay [2-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide]. Extraction of cacao pods were obtained using the ultrasonic homogenizer method with ethanol 70%. The cytotoxicity test was observed for 24 hours and the doubling time proliferative test was observed for 24, 48, and 72 hours. Result: Cocoa pod husk extract showed there were no cytotoxic effects with viability of primary cells of periodontal ligament fibroblasts at the concentration of 1.56 pg/ml, 3.125 pg/ml, 6.25 pg/ml, 12.5 pg/ml, 25 pg/ml, 50 pg/ml, and 100 pg/ml. The proliferation test showed increasing of fibroblasts at 24 and 48 hours. Kruskal Wallis analysis obtained a significance value (p<0.05) Conclusion: Cytotoxicity and proliferation assays of Cocoa pod husk extract increased cell viability and cell proliferation of periodontal ligament fibroblast.
The Effect of Proanthocyanidin Cocoa Pod Rind Extract (Theobroma cacao L.) on MMP-8 Expession in Gingival Tissue of Periodontitis Rats Model Yani Corvianindya Rahayu; Agustin Wulan Suci Dharmayanti; Anya Tania Larasati
Denta Journal Kedokteran Gigi Vol 14 No 2 (2020): Agustus
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/denta.v14i2.6

Abstract

Background: Periodontitis is periodontal tissue inflammation due to Porphyromonas gingivalis which invades the gingival tissue due to the activity of virulence factors. This causes the host immune system changes and increases MMP-8 production in periodontal tissue so resulting excessive tissue destruction. Cocoa pod rind containing proanthocyanidin compound as anti-inflammatory drugs is given to reduce MMP-8 and cure periodontitis. Objective: To examine the effect of proanthocyanidin extract of cocoa pod rind (Theobroma cacao L.) on decreasing MMP-8 expression in gingival tissue of periodontitis rats model. Materials and Methods: The samples used were 16 male Wistar rats divided into 2 groups, placebo gel for the control group and proanthocyanidins gel for the treatment group. ). Each group was induced by P.gingivalis bacteria every 3 days for 14 days and then smeared with placebo gel and proanthocyanidin gel for 7 and 14 days. Then all rats’ gingival tissue were taken and made preparations to be observed under a microscope and analyzed using immunoratio software. Result: There were differences in MMP-8 expression based on observation time (p<0.05). MMP-8 expression in the 7th day control group was lower than the 14th day and MMP-8 expression in the 7th day treatment group was higher than the 14th day. Conclusion: Proanthocyanidin of cocoa pod rind extract (Theobroma cacao L.) can reduce MMP-8 expression in gingival tissue of periodontitis rats model.
THE CYTOTOXIC AND PROLIFERATIVE ACTIVITY OF COCOA POD HUSK EXTRACT (THEOBROMA CACAO L.) ON PERIODONTAL LIGAMENT FIBROBLASTS Rahayu, Yani Corvianindya; Triwahyuni, Iin Eliana; Kusumawardani, Banun; Sari, Dewi Yunita
Odonto : Dental Journal Vol 9, No 1 (2022): July 2022
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.9.1.46-52

Abstract

Background: Theobroma cacao was considered agricultural wastes. Cocoa pod husk contain various bioactive compounds as natural antioxidants,antibacterial and anti-inflammatory. A good biomaterial must be non-toxic and have no detrimental effect on the biological environment. The aim of research was to determine the cytotoxicity and proliferative activity of cocoa pod husk extract on primary cells of periodontal ligament fibroblasts. Method: This experimental laboratory used primary cell culture of periodontal ligament fibroblasts. The cytotoxicity and proliferative activity were analyzed by MTT assay [2-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide]. Extraction of cacao pods were obtained using the ultrasonic homogenizer method with ethanol 70%. The cytotoxicity test was observed for 24 hours and the doubling time proliferative test was observed for 24, 48, and 72 hours. Result: Cocoa pod husk extract showed there were no cytotoxic effects with viability of primary cells of periodontal ligament fibroblasts at the concentration of 1.56 pg/ml, 3.125 pg/ml, 6.25 pg/ml, 12.5 pg/ml, 25 pg/ml, 50 pg/ml, and 100 pg/ml. The proliferation test showed increasing of fibroblasts at 24 and 48 hours. Kruskal Wallis analysis obtained a significance value (p<0.05) Conclusion: Cytotoxicity and proliferation assays of Cocoa pod husk extract increased cell viability and cell proliferation of periodontal ligament fibroblast.
Co-Authors Affian Hudatama Putra Agung Sosiawan Agustin Wulan Suci Dharmayanti Anis Irmawati Anya Tania Larasati Atik Kurniawati Atik Kurniawati, Atik Banun Kusumawardani Budi Yuwono Devi Komala Dewi Yunita Sari Didin Erma Indahyani Dwi Merry Christmarini Robin Dwi Merry Christmarini Robin Dyah Setyorini Ernie Maduratna Setiawatie Falah, Pilar Menara Fitriana Wadianur Fitriyana, Nurul Isnaini Iin Eliana Triwahyuni Izzata Barid Kristanti, Yuli Dwi Kumala, Efa Ayu Ratnaning Luh Putu Ratna Sundari Lusi Hidayati, Lusi Mei Syafriadi Muhammad Nurul Amin Parawita Dewanti Parawita Dewanti Rahmat, Naila Azifatur Ramadan, Doaa Elsayed Retno Pudji Rahayu Rudi Joelijanto Sheila Dian Pradipta Siti Komariyah TRI HANDOYO Vievien - Widyaningtyas Wachju Subchan Yudha Nurdian Zainul Cholid