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All Journal Jurnal Pengolahan Hasil Perikanan Indonesia Jurnal Penelitian Saintek ILMU KELAUTAN: Indonesian Journal of Marine Sciences Bioma : Berkala Ilmiah Biologi BERKALA FISIKA JURNAL ANATOMI FISIOLOGI SAINS DAN MATEMATIKA Jurnal Natur Indonesia Jurnal Akademika Biologi Teknosains: Media Informasi Sains dan Teknologi Prosiding KPSDA Jurnal Bioteknologi & Biosains Indonesia (JBBI) Indonesian Food and Nutrition Progress Biosaintifika: Journal of Biology & Biology Education Berkala Bioteknologi Jurnal Biologi Tropika NICHE Journal of Tropical Biology Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) BIOEDUSAINS: Jurnal Pendidikan Biologi dan Sains Jurnal Energi Baru dan Terbarukan AGRIC
Endang Kusdiyantini
Departemen Biologi Fakultas Sains Dan Matematika, Universitas Diponegoro, Semarang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences

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Eksplorasi Mikroba Penghasil Enzim-enzim Hidrolitik Di Kawasan Taman Nasional Lore Lindu Sulawesi Tengah Rahmiati, Rahmiati; Pujianto, Sri; Kusdiyantini, Endang
Bioma : Berkala Ilmiah Biologi Vol. 18, No.1, Tahun 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (47.46 KB) | DOI: 10.14710/bioma.18.2.14-19

Abstract

Lore Lindu National Park (TNLL) is an area that  flora, fauna and microbes, the diversity of microbes producing hydrolytic enzyme. explore the hydrolytic enzyme producing microbes in Indonesia. This study was aimed to obtain bacterial isolates were able to produce hydrolytic enzyme and characteristics. Isolation in the microbiology laboratory. Isolation  by a spread plate. Isolates in the selection hydrolytic enzyme producing selective media. Measurement of the activity of the enzyme with hydrolytic index. The results  were thirteen isolates clearing zone test , 2 protease enzyme bacterial isolates, 1 lipase enzyme bacterial isolates, 6 amylase enzyme bacterial isolates, while 4  cellulase enzyme bacterial isolates. Examination of Amylase enzyme activity was done using DNS method. L10T3 showed that the bacterial isolate optimum activity at pH 7 and at a temperature of 300C with an activity of 0.040 U / mL and 0.029 U / mL. Key word: Lore Lindu National Park, hydrolytic enzyme. isolation of bacteria
Potensi Tepung Umbi Dahlia Dan Ekstrak Inulin Dahlia Sebagai Sumber Karbon Dalam Produksi Fruktooligosakarida (FOS) Oleh Khamir Kluyveromyces marxianus DUCC-Y-003 Yuliana, Rida; Kusdiyantini, Endang; Izzati, Munifatul
Bioma : Berkala Ilmiah Biologi Vol. 16, No.1, Tahun 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (409.155 KB) | DOI: 10.14710/bioma.16.1.39-49

Abstract

Penelitian ini bertujuan untuk mengeksplorasi manfaat umbi dahlia dalam bentuk tepung umbi dan ekstrak inulin sebagai substrat untuk memproduksi FOS. Produksi FOS berlangsung secara mikrobial enzimatis dengan bantuan khamir Kluyveromyces marxianus DUCC-Y-003. Pengkulturan khamir pada media kultur berlangsung selama 60 jam dan dilakukan pengukuran tiap 6 jam. Pengukuran tersebut meliputi pertumbuhan khamir, kadar fruktosa medium dan kadar total gula medium. Penentuan FOS dilakukan dengan cara mengukur nilai derajat polimerisasi (DP). DP merupakan hasil perbandingan antara kadar total gula dengan kadar fruktosa pada media kultur. DP FOS berkisar antara 2-10 dan FOS komersil dengan DP 3-5. Penelitian menggunakan Rancangan Acak Kelompok Faktorial (RAKF). Perlakuan terdiri atas S1D1 (substrat tepung, dosis 1 g), S1D2 (tepung, 3 g), S1D3 (tepung, 5 g), S2D1 (ekstrak inulin, 1 g), S2D2 (ekstrak inulin, 3 g), S2D3 (ekstrak inulin, 5 g). Hasil penelitian menunjukkan bahwa pertumbuhan khamir tertinggi pada perlakuan penggunaan ekstrak inulin dengan dosis 1 gram, dan pertumbuhan terendah pada perlakuan penggunaan tepung dengan dosis 5 gram. Pada masing-masing perlakuan, menghasilkan produk FOS dengan nilai DP yang beragam selama waktu inkubasi 60 jam, hanya perlakuan S2D1 (ekstrak inulin, 1g) yang sudah menghasilkan ketiga jenis FOS komersil dalam waktu inkubasi 60 jam. Pada perlakuan S2D1, produk 1-kestosa dihasilkan saat inkubasi 42 jam, produk nystosa saat 48 jam dan produk fruktofurasylnystosa saat 60 jam. Kesimpulan hasil penelitian adalah perlakuan S2D1 paling efektif dalam menghasilkan FOS selama waktu inkubasi 60 jam, sedangkan perlakuan lain dapat menghasilkan FOS yang sama jika lama waktu inkubasi diperpanjang.   Kata kunci : inulin, inulinase, derajat polimerisasi, Kluyveromyces marxianus DUCC-Y-0003
Penambahan Filtrat Tepung Umbi Dahlia (Dahlia variabilis Willd.) sebagai Prebiotik dalam Pembuatan Yoghurt Sinbiotik Alfaridhi, Khabib Khasan; Lunggani, Arina Tri; Kusdiyantini, Endang
Bioma : Berkala Ilmiah Biologi Vol. 15, No.2, Tahun 2013
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (548.421 KB) | DOI: 10.14710/bioma.15.2.64-72

Abstract

Yoghurt is a fermented milk product that can provide beneficial effects to the health of consumers. Increasing the quality of yoghurt can be made by combining the benefits of probiotic with prebiotic to produce sinbiotic yoghurt. Inulin is a prebiotic that found in many dahlia tuber (Dahlia variabilis Willd.). Pure inulin were expensive, so the alternative is using dahlia tuber flours filtrate as prebiotic ingredients. This study aimed to examine the addition of Dahlia Tuber Flours Filtrate (DTFF) as a prebiotic in sinbiotic yoghurt making using Lactic Acid Bacteria (LAB) Lactobacillus bulgaricus, Streptococcus termophilus dan Lactobacillus acidopilus as probiotic. This research using Completely Randomized Design (CRD) with 4 concentration treatments DTFF 0%, 3%, 6% and 9% with 3 replications. Observed variables consist of the number of total LAB, lactic acid level, pH and organoleptic attributes (aroma, flavor, consistency and impression). Data the number of total LAB, lactic acid level and pH values ​​were analyzed by ANOVA, continued by Duncan test, while the organoleptic data was analyzed by Mann-Whitney U test with 95% significant level. The results showed that the addition of DTFF significantly affect the increasing of total LAB, lactic acid level, organoleptic assessment and decreasing pH of sinbiotic yoghurt. Sinbiotic yoghurt with 3% of DTFF concentration is most preferred by panelists and required the criteria of Indonesian National Standard (SNI) for yoghurt with enough likely aroma, sour taste/specify and enough thick texture with total LAB 2,4 x 108 CFU/ml, 1.3% lactic acid levels and 4.2. pH. Keywords : prebiotic, probiotic, inulin, dahlia tuber, sinbiotic yoghurt, lactobacillus bulgaricus, streptococcus termophilus, lactobacillus acidopilus
Pengaruh Kandungan Ekstrak Daun Zodia (Evodia suaveolens) Terhadap Mortalitas Larva Nyamuk Aedes aegypti Basundari, Sinta Anas; Tarwotjo, Udi; Kusdiyantini, Endang
Bioma : Berkala Ilmiah Biologi Vol. 20, No. 1, Tahun 2018
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (61.693 KB) | DOI: 10.14710/bioma.20.1.51-58

Abstract

Aedes aegypti mosquito is a vector of dengue hemorrhagic fever (DHF). One place that is endemic by A.aegypti mosquitoes is the city of Semarang, specifically  in Tembalang District. Tembalang district is one of the areas with highest DHF incidence rate. A.aegypti mosquito vector control is still using artificial insecticide, where the insecticide has negative impact to health and environment. Zodia leaves (Evodia suaveolens) are known to have potential as a natural insecticide, because they have secondary metabolites such as tannins, alkaloids, flavonoids that can be used as biolarvasides. This study aims to determine the content of phytochemical compounds of zodia leaves extract and to know the mortality in each population to zodia leaves extract. The extract of zodia leaf is obtained using maceration method. Chemical compounds contained in zodia leaves were analyzed using FT-IR. This research method is bioassay test, where the larvae are soaked with various series of concentration of zodia leaf extract. Each series of concentrations required ten larvae. The larvae used came from five kelurahan in Tembalang Subdistrict. The data were processed using probit analysis to obtain LC50. The results of LC50 obtained from the smallest to largest is 39,94 (34,67-45,28); 41,98 (36,6-48,07); 46,18 (39,28- 53,97); 47,62 (39,92-54,32) and 49,44 (42,24-58,25). The result of LC50 shows that is not significantly different, because there is the value of the overlapping fiducial limits between each population
Kualitas Simplisia Tanaman Biofarmaka Curcuma domestica Setelah Proses Pemanasan Pada Suhu Dan Waktu Bervariasi Kusumaningrum, Hermin Pancasakti; Kusdiyantini, Endang; Pujiyanto, Sri
Bioma : Berkala Ilmiah Biologi Vol. 17, No.1, Tahun 2015
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2518.981 KB) | DOI: 10.14710/bioma.17.1.27-33

Abstract

Curcuma domestica is one of traditional medicinal plants that is found in Gunungpati Semarang. However the dried product do not achieve optimal standard quality for simplicia  in terms of microbial contaminant and in an industrial scale household. Knowledge on how to use  sterilization   to produce better simplicia and reducing contaminant has not been known by farmers, yet. The purpose of this activity was to obtain the best heating treatment on sterilization of Curcuma simplicia using several temperature under sunlight and oven device. It was also want to  show microbial growth after heating at several times and their  influence on the quality of simplicia after treatment. The method was conducted by  simplicia sterilization of C. domestica using sunlight sterilization for a week and using oven at a temperature of 300C, 400C, 500C and 600C for 1, 3, 6, 9, 12, 24, and 48 hours. The results showed that heating at temperature of 500C for 48 hours obtained the best simplicia, followed by heating at a temperature of 600C for 16-48 hours without contaminants after storing period for 3 months.   Key words :Curcuma, sterilization, heat, microbia
Optimasi Produksi Inulinase isolat P 12 pada Tepung Umbi Dahlia ( Dahlia variabilis Wild ) dengan Variasi Konsentrasi Nitrogen Organik dan Waktu Inkubasi Lunggani, Arina Tri; Wijanarka, Wijanarka; Kusdiyantini, Endang
Bioma : Berkala Ilmiah Biologi Vol. 12, No. 1, Tahun 2010
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (49.893 KB) | DOI: 10.14710/bioma.12.1.20-23

Abstract

Efforts to address health problems mainly related to the digestive tract is by consuming one prebiotic, eg fruktooligosakarida (FOS). FOS is a prebiotic that one species can be produced from the hydrolysis of inulin using inulinase enzyme. Isolate P12 is an isolate that has been proven to have high inulinase activity on standard medium inulin production. Inulinase production increase can be done by adding a source of organic nitrogen in the form of yeast extract in medium. The results indicate that the best on the concentration of nitrogen concentration P2 (Yeast extract 0.25%) with the activity of 0.7983 IU, while the best 12-hour incubation time with the activity of 0.7899 IU. Likewise for the best interaction P2 T2 treatment with inulinase activity of 0.9025 IU.
Identifikasi Jenis Pigmen Dan Uji Potensi Antioksidan Ekstrak Pigmen Bakteri Serratia marcescens Hasil Isolasi Dari Sedimen Sumber Air Panas Gedong Songo Naufal, Adhitya; Kusdiyantini, Endang; Raharjo, Budi
Bioma : Berkala Ilmiah Biologi Vol. 19, No. 2, Tahun 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (231.736 KB) | DOI: 10.14710/bioma.19.2.95-103

Abstract

Pigments can be produced by plants, animals, and microbes, including the bacteria Serratia marcescens. Pigments are believed to be used as antibacterial, anticancer, antibiotic and antioxidant. The quality of the pigment is greatly determined by its type. The aim of this study was to identify the type of pigment produced by S. marcescens resulting from the sediment of Gedong Songo hot spring, and testing its antioxidant activity. S. marcescens was grown on Nutrient Broth (NB) medium for 96 hours at room temperature and sampled once every 6 hours for growth measurement and pigment measurement. Cell dry weight was used to measure the growth of S. marcescens, while the measurement of pigment production was done using spectrophotometer with 535 nm wavelength for red pigment and identification of pigment with Thin Layer Chromatography and UV-Vis and testing of antioxidant activity using carotene bleaching method. The results showed the measurement of pigment production has optimal point at 48 hours at 1,319 mg/L. Identification of pigment type S. marcescens using TLC obtained value of  0,8 with spectrophotometer wavelength at 536 nm. The value of antioxidant activity Pigment S. marcescens obtained at 13%. Keywords: Serratia marcescens, prodigiosin, KLT, antioxidant
Isolasi dan Identifikasi Bakteri Pelarut Fosfat dari Tanah Gambut Larasati, Ella Dewani; Rukmi, MG Isworo; Kusdiyantini, Endang; Ginting, R Cinta Badia
Bioma : Berkala Ilmiah Biologi Vol. 20, No. 1, Tahun 2018
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (383.652 KB) | DOI: 10.14710/bioma.20.1.1-8

Abstract

Phosphate (P) are macronutrients that necessary for growth and development of plants, But the availability of phosphate dissolved in the ground are very limited because trend is bound to minerals ground form phosphate complexs. One of the alternative to improve the efficiency of phosphate is by utilize phosphate solubilizing bacteria. Phosphate solubilizing bacteria is bacteria capable of dissolving phosphate that is not available become available, so that it can be absorbed by plants. Peat soils can be used as a source of phosphate solubilizing bacteria because , peat soils formed from litter and organic matter so, many microorganisms which live in peat soils one of them is a phosphate solubilizing bacteria. The aim of this research is to isolat, identify bacteria to species level using Biolog system GenIII Mircoplate, and test the ability of phosphate dissolution. The isolation is done with the methods spread plate in a media pikovkaya solid and testing the ability to isolat qualitatively glimpsed clear zone around colonies, measuring phosphate solubilizing index. testing the ability isolats quantitatively by measuring solubility phosphate using spektrofotometer, and identification isolats using Biolog System. Eighteen isolates phosphate solubilizing bacteria successfully obtained from of peat soils, two isolates of them potential in solubilizing phosphate, According to Biolog system the second isolates identified as Pseudomonas tolaasii (isolate PG2T.5) and a Bacillus pumilus (isolate PG3TT.2), each is about phosphate dissolved successive of 24,81 mg/L and 22,62 mg/L Key words: Peat Soil, Isolation, Phosphate Solubilizing  Bacteria, Biolog System Identification
Penggunaan Substrat Glukosa Berbagai Konsentrasi sebagaiSumber Karbon Microbial Fuel Cell Saccharomyces cerevisiae untuk Menghasilkan Energi Listrik Nurhakim, Muhammad Amal; Kusdiyantini, Endang; Raharjo, Budi
Bioma : Berkala Ilmiah Biologi Vol. 18, No.2, Tahun 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (81.988 KB) | DOI: 10.14710/bioma.18.2.131-136

Abstract

The increases of human growth causes electrical energy demand’s expantion while the supply decreases drastically. Energy crisis had triggeredalternative renewable energy sourcesdevelopmentto substitutethe use ofoil that had beenmain energy resources for the people. Microorganisms utilization is used to produce electrical by researchers these years as an effort to actualize the goals. The system used is microbial fuel cell (MFC) technology which utilize metabolism activity from microorganisms to produce electrical energy. Microorganismswill perform metabolism bybreaking down glucose into hydrogen (H2) and oxygen (O2).Hydrogen has a role as raw material that used in reduction reaction with oxygen until it releases electron in anoda as electrical flows source. Saccharomyces cerevisiae is an example microorganisms that can utilize for produce electrical energy. This research aims to  find optimal concentration for glucose as a carbon source in microbial fuel cell Saccharomyces cerevisiaeto form electrical energy. This research use S. cerevisiae as microorganisms and variation of glucose concentration as a carbon source. Parameters measured in this study is the voltage (mV) and current (mA). Research’s result shows that glucose in 10 % (w/v) concentrate forms higher results in voltage (mV) and current (mA) compare to glucose with 20% (w/v) concentrate and in the concentrate of 30% (w/v) which values each 561,833 mV and 105,133 mA. Analysis of variance with level of confidence 95% shows glucose concentrates don’t react significantly voltage but react significantly on current. Tukey HSD’s test show significant different between current that was formed by glucose in the concentrate of 10% (w/v) compared to glucose in the concentrate of 20% (w/v) and 30% (w/v).Keywords : Saccharomyces cerevisiae, microbial fuel cell (MFC), glucose, electrical energy
Kajian Efisiensi dan Karakterisasi Produksi Ozon dengan Lucutan Plasma Berpenghalang Dielektrik (DBDP) untuk Pengendalian Jamur dalam Beras Rachman, Dian Arif; Nur, Muhammad; Kusdiyantini, Endang
BERKALA FISIKA 2014: Berkala Fisika Vol. 17 No. 1 Tahun 2014
Publisher : BERKALA FISIKA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (98.163 KB)

Abstract

A research about the influence of ozone on fungi  which living on rice type mentik that used natrium agar (TPC methode) media has done.  The research was done at normal temperature and the outside air pressure with free air as a source of ozone. The ozone concentration of 2.91 up to 3.19 ppm was radiated on 7 differnet samples of rice, each sample 50 gram used ozonized  time variation 0 minutes, 30 minutes, 60 minutes, 90 minutes, 120 minutes, 150 minutes and 180 minutes. It was obtained results the number of fungi that died of 97,3 % after ozone irradiated for 30 minutes and 99,2% after 60 minutes. Total number of fungi which has dead during 150 minutes as much 99,9 %. Characterization of ozone conducted using ozone reactor (Dielectric Barrier Discharge Plasma). variation in the voltage and the rate of air flow was performed to obtain the optimum concentration of ozone. Voltage variation started from 6 kV, 6 kV, 8 kV and 6 kV whereas variation of oxygen flow rate of 0.5 L/min, 1 L/min, 2 L/min and 3 L/minute. The results obtained with the same tendency which is that at the same voltage, the higher the air flow rate and declining concentrations of ozone. On the study for flow rate of 0.5 L/min the ozone concentrations was obtained 4.6 up to 6.8 ppm. While for flow rate of 3 L/minute ozone concentrations was obtained 2.9 up to  4.8 ppm. Keywords: fungi, natrium agar, irradiate, reactor
Co-Authors Adde Lolita Putri Adhitya Naufal Pribadhi Afrazak Johansyah Agni Rizqy Berliyanti Agung Suprihadi Ahmad Thontowi Anggraini, Ika Anggraini, Ika Anggraini, Ika Anggraini Anggrayeni, Yesti Tri Anto Budiharjo Arina Lunggani Arina Tri Lunggani Arina Tri Lunggani Arina Tri Lunggani Atit Kanti Ayuningtyas, Annisa Nur Azzahra, Meilidya Falkhiya Basundari, Sinta Anas Bintoro Rudi Saputro, Bintoro Rudi Budi Raharjo Daniel Pasaribu Devia Kusmawati Arfina Dian Arif Rachman E Hugeng Wandono Eka, Prastya Muhammad Elawati, Nunung Eni Elawati, Nunung Eni Enny Yusuf Wachidah Yuniwarti Erma Prihastanti Euis HERMIATI Evi F Simanjuntak Faradila Ayu, Near Putri Fransenda, Auxensius Rexer Ginting, Ledy Ginting, R Cinta Badia H Hadiyanto Hadi Endrawati Hapzi Ali Herida, Azalia Puspa Hermin P Kusumaningrum Hermin P. Kusumaningrum Hermin Pancasakti Kusumaningrum Hilmi Fadhli Ihdina Isfara Suteja Ika Anggraini Ika Anggraini Indah Sulistyarini Joedoro Sudarsono Khabib Khasan Alfaridhi, Khabib Khasan Kumudasmoro, Bagas Mukti Kurniawati, Laily Laily Kurniawati Larasati, Ella Dewani Maria Sarah Fadillah MG Isworo Rukmi Moi, Maria Yasinta Muhammad Amal Nurhakim, Muhammad Amal Muhammad Nur Muhammad Nur Muhammad Z ainuri Muhammad Zainuri Munifatul Izzati N Nurhayati Naufal, Adhitya Nia Fadlilatul Laily Novi Alvita Pratama Nugrahaini, Dian Laila Nunung Eni Elawati Nunung Eni Elawati Nurhayati Nurhayati Nurhayati Nurhayati Nurhayati Nurhayati Nurmila, Ika Oktavia Pardede, Lasria Pratama, Novi Alvita Prayitno, S Feta Avila Prianto, A. Heru Puteri Aryani Putri, Adde Lolita Octavia Rejeki Siti Ferniah Rida Yuliana, Rida Safitri, Linda Saniha Adini, Saniha Sarjana Parman Setiawan Wicaksono, Setiawan Siregar, Mangasa Tua Pandapotan Soni Nugraha Sri Pujianto, Sri Sri Pujiyanto Suprihadi Suryaningsih, Vivi Sutaryo TATI NURHAYATI Tri Winarni Agustini Triwibowo Yuwono Tyas Rini Saraswati Udi Tarwotjo, Udi Wijanarka Wijanarka Wijanarka, W Yeti Darmayati