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All Journal Buletin Peternakan Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Indonesian Journal of Biotechnology Elkawnie Livestock and Animal Research Animal Production : Indonesian Journal of Animal Production Indonesian Journal of Chemistry Jurnal Riset Agribisnis dan Peternakan Agroindustrial Journal
Lies Mira Yusiati
Department Of Animal Nutrition And Feed Science, Faculty Of Animal Science, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Social Sciences Veterinary Other

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Journal : Agroindustrial Journal

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Molecular Identification of Lactic Acid Bacteria from Broiler Chicken Meat Mudawaroch, Roisu Eny; Setiyono, Setiyono; Yusiati, Lies Mira; Suryanto, Edi
Agroindustrial Journal Vol 10, No 2 (2023)
Publisher : Department of Agroindustrial Technology, Faculty of Agricultural Technology UGM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/aij.v10i2.89982

Abstract

Lactic acid bacteria are bacteria used to ferment food. Molecular identification of isolates of lactic acid bacteria has a high degree of accuracy compared to morphological identification. This study aimed to molecularly identify isolates of lactic acid bacteria from broiler chickens. Molecular identification is carried out in 3 stages, namely: rRNA gene amplification stage, squencing and phylogeny tree. PCR electrophoresis results obtained amplicone results measuring about 1,500 bp. The results of BLAST analysis showed that the gen 16S rRNA sequence of BR 17 lactic acid bacteria isolate had a 97-98% similarity with the genome sequence of the Lactobacillus fermentum strain.  Development of phylogenetic tree isolates of single clusters of lactic acid bacteria BR 17 with strains of Lactobacillus fermentum CAU2036, Lactobacillus fermentum JCM 7772, and Lactobacillus fermentum 10-18. The identification results of lactic acid bacteria isolate BR 17 showed Lactobacillus fermentum BR 17.
Co-Authors Abbas (Abbas) Achmad Dinoto Achmad Dinoto Adhe Humaera Adi Magna Patriadi Nuhriawangsa Agustinah Setyaningrum, Agustinah Ahmad Sofyan Ali Agus Ali Agus Ali Agus Ali Agus Ali Wibowo Ali Wibowo Ali Wibowo Ambar Pratiwiningrum Andriyani Astuti Asih Kurniawati Asih Kurniawati B. Suhartanto Bambang Suhartanto Budi Prasetyo Widyobroto Canadianti, Monica Catur Suci Purwati Chusnul Hanim Diah Tri Widayati Dianestu Putra Edi Suryanto Edi Suryanto Edi Suryanto Edy Suryanto Efka Aris Rimbawanto Ema Damayanti Ema Damayanti Endang Baliarti Erika Kusumawardani Fandi Widya Rachman Firman Nasiu Gardika Windar Prahara H. Hartadi Hari Hartadi Harwanto (Harwanto) I Gede Suparta Budisatria I Gede Suparta Budisatria Ika Sumantri Jamhari (Jamhari) Joko Riyanto Koestantinah Koestantinah, Koestantinah Kunty Novi Gamayanti Kustantinah Kustantinah Lilis Hartati Ludfia Widyasmara mudawaroh, roisu Mudawaroh, Roisu Eny Mugiono (Mugiono) Muhamad Nur Cahyanto Muhamad Nur Cahyanto, Muhamad Nur Muhammad Nur Cahyanto Muhsin Al Anas Nafiatul Umami Nafly C. Tiven Nafly C. Tiven Novita Kurniawati Puput Diah Sitoresmi Rahma Fitriastuti Ristanto Utomo Ristianto Utomo Ristianto Utomo Ristianto Utomo Roisu Eny Mudawaroch Roisu Eny Mudawaroch Roisu Eny Mudawaroch Roisu Eny Mudawaroch Rusman (Rusman) Rusman Rusman Rusman Rusman Sapta Chandra Marnadi Hidayat Setiyono (Setiyono) Setiyono Setiyono Setiyono Setiyono Setiyono Setiyono Setiyono Setiyono Setiyono, Etiyono Soemitro Padmowijoto Soeparno Soeparno Soeparno Soeparno Subur Priyono Sasmito Budhi Suharjono Triatmojo Sujadi (Sujadi) Sulvia Dwi Astuti Supadmo (Supadmo) Suryanto, Edi Syamsul Arifin Tety Hartatik Tri Yuwanta Umar Santoso Umar Santoso Umar Santoso Wahidin Teguh Sasongko Wayan Tunas Artama Wayan Tunas Artama Wibowo, Siti 'Athiya Widayati, Okti Widodo Widodo Widodo Widodo Yantyati Widyastuti Zaenal Bachruddin Zaenal Bachrudin