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3. Lactobacillus casei Fermented Milk as a Treatment for Diabetes in Mice (Mus musculus) Pratiwi Purnama Sari; Nurliana Nurliana; M. Hasan; Arman Sayuti; Sugito Sugito; Amiruddin Amiruddin
Jurnal Medika Veterinaria Vol 11, No 1 (2017): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v11i1.4088

Abstract

The study aimed to find out the effect of Lactobacillus casei fermented milk as diabetic drug (Therapeutic). Mice were 3-4 month old, male and body weight ranged 20-30 g. This Research used Completely Randomized Design with factorial pattern, consisted of dose and  duration of  feeding. Treatments were divided into four treatment, P1 (no treatment), P2 (mice were induced with alloxan), P3 (mice were induced with alloxan and fed with fermented milk L. casei with 0.5 mL/mice) and P4 (mice were induced with alloxan and fed with Lactobacillus casei fermented milk 1 mL/mice). Provision of L. casei fermented milk provided at libitium and L. casei fermented milk given for 7 and 14 days. Blood  were collected from Vena lateralis and dropped on the strip test Easy Touch GGHb. The results showed that not only dose administration of L. casei fermented milk had significantly decrease (P0.01) of glucose levels in mice, but also duration of treatment. It can be concluded that the administration of fermented milk L. casei can be used as a complement therapy for diabetic mice
8. Histopathological of Fabricius Bursa in the Broiler Chicken after Vaccination with Newcastle Disease Virus Vaccine Ummu Balqis; Nurjannah Annah; Hamdani Budiman; Darmawi Darmawi; Cut Dahlia Iskandar; M. Hasan
Jurnal Medika Veterinaria Vol 13, No 1 (2019): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v13i1.4178

Abstract

The aim of this research was to determine an effect of Newcastle disease virus (NDV) vaccine to histopathologic on Fabricius bursa of broiler chicken at various age levels. Eighteen commercial DOC (Day Old Chick) were divided into 2 groups namely a control group that was given distilled water and a treatment group that was given NDV vaccine namely ND Lasota vaccine ocularly as much one drop per eye at day 3, then a booster dose in drinking water at day 13 was given. The bursa of Fabricius was collected from these chickens on the age of 14 days, 21 days, and 28 days after sacrificing the chickens through necropsied, then histopathological preparations are made using hematoxylin-eosin staining. The parameters observed were measured the number, diameter, and thickness of cortical of lymphoid follicles. Data were analyzed descriptively and quantitatively by unpaired t-test. The results of this research showed the NDV vaccine effects were not significantly different (P0.05) to the number and diameter of lymphoid follicles. In the treatment group, 14 days old broiler chickens had a decreased number of lymphoid follicles, while its diameter tends to grow and the thickness of cortex was also seemed unchanged compared with the control group. Then at the age of 21 days, the number, diameter, and thickness of cortex of lymphoid follicles tend to increase, while at 28 days diameter of lymphoid follicles tend to be slightly reduced, but the number and thickness tend to grow out of control. It was concluded that the NDV vaccine had a good effect on the Fabricius bursa of broiler chickens with increasing age, then histopathological preparations are made using hematoxylin-eosin staining.
PEMERIKSAAN BADAN KETON URINE PADA ORANGUTAN SUMATERA (Pongo abelli) MENGGUNAKAN STRIP TEST SEMI KUANTITATIF DI PUSAT REINTRODUKSI CAGAR ALAM PINUS JANTHO (Ketone Bodies Identification on Sumatran Orangutan’s (Pongo abelli) Urine Using Semiquantitative Strip Test at Reintroduction Centre of Pine Jantho Nature Reserve) Awaluddin Awaluddin; Nuzul Asmilia; Erdiansyah Rahmi; Triva Murtina Lubis; M. Hasan; Rusli Rusli
Jurnal Medika Veterinaria Vol 9, No 2 (2015): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v9i2.3809

Abstract

This research was aimed to study the presence of ketone bodies in urine of sumatran orangutan as a medical record of orangutan at new reintroduction centre of pine nature reserve Jantho, Aceh Besar. Urine was collected from 8 individual sumatran orangutan (Kiskis, Amin, Mawasudin, Marconi, Ruben, Ayu, Yusniar, and Coty) which placed in individual cage and semiwild orangutan. Sampling was carried out in the morning after the sample going out of nest or before given the first feed for individual inside the cage. Sample was collected 4 times with three repetitions in 4 weeks from June to July 2013. The examination of urine was conducted subsequently after urine collection by dipped the striptest in 5 mL of urine for 60’second. Data were analyzed using descriptive qualitative method. The semiquantitative results of ketone body was obtained by striptest with 5 scale of colors change: negative (0 mg/dL), trace (5 mg/dL), small (15 mg/dL), moderate (40 mg/dL), and large (60 mg/dL-180 mg/dL). The results showed that three individuals placed in the cage (Kiskis, Amin, and Mawasudin) were negative for ketone bodies. In contrast for 5 semiwild orangutans namely Marconi was negative for ketone bodies, Ruben was positively detected for ketone bodies found in the third week (M3) with levels of 5 mg/dL, Ayu was positively detected for ketone bodies in the first week (M1) with levels of 15 mg/dL and in the second week (M2) with a concentration of 5 mg/dL, Yusniar was positively detected for ketone bodies with levels of 5 mg/dL in the first week (M1) and fourth week (M4), and Coty was positively detected for ketone bodies with levels of 5 mg/dL in the second week (M2) and fourth week (M4).Key words: orangutan, ketone bodies, reintoduction, urinalysis
IDENTIFIKASI PARASIT NEMATODA SALURAN PENCERNAAN ANJING PEMBURU (Canis familiaris) DI KECAMATAN LAREH SAGO HALABAN PROVINSI SUMATERA BARAT Desi Akhira; Yudha Fahrimal; M. Hasan
Jurnal Medika Veterinaria Vol 7, No 1 (2013): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v7i1.2919

Abstract

Penelitian ini bertujuan mengetahui jenis-jenis parasit cacing nematoda yang menginfestasi saluran pencernaan serta derajat infestasinya pada anjing pemburu (Canis familiaris) di Kecamatan Lareh Sago Halaban, Sumatera Barat. Penelitian ini menggunakan sampel feses dari 70 ekor anjing pemburu jantan. Penelitian ini mengunakan metode sentrifus untuk mengidentifikasi telur cacing nematoda serta metode Mc Master untuk penghitungan jumlah telur cacing nematoda. Hasil penelitian menunjukkan bahwa dari 70 ekor anjing pemburu di Kecamatan Lareh Sago Halaban, Sumatera Barat ditemukan sebanyak 40 ekor anjing pemburu (57,14%) positif mempunyai telur cacing nematoda gastrointestinal dengan jenis infeksi tunggal oleh Ancylostoma spp. Uncinaria spp. dan Toxocara spp., infeksi ganda oleh Ancylostoma spp. dan Uncinaria spp. serta Ancylostoma spp. dan Toxocara spp.dan multi infeksi oleh ketiga jenis cacing tersebut serta 30 ekor anjing pemburu (42,85%) tidakterinfeksi cacing. Dari 40 anjing yang terinfestasi tidak terdapat yang bersifat berat, dan 3 ekor menderita infestasi sedang dan selebihnya bersifat ringan.
16. The Number Of Leukocyte And Leukocyte Differential In Broilers That Infected With Eimeria tenella And Given Neem Leaf Extract And Jaloh Extract Rizki Aulia; Sugito Sugito; M. Hasan; T. Fadrial Karmil; Gholib Gholib; Rinidar Rinidar
Jurnal Medika Veterinaria Vol 11, No 2 (2017): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v11i2.4667

Abstract

The aim of this research was to determine the effect of giving neem leaf extract combined with jaloh towards the total of leukocytes and differential leukocyte in broilers that was infected with Eimeria tenella. This research samples were 20 Cobb stain broilers 14 days old. Design the research was complete randomized design with five treatments and each treatment consists of four repetitions. First treatment (P1) as negative control which was only given mineral water; second treatment (P2) as positive control was is given 5 mg/L anti-stress commercial in drinking water; Third treatment (P3) was given 1000 mg/L Jaloh extract; Fourth treatment (P4)was given 250mg/L neem extract; and fifth treatment (P5)was given 1000mg/L jaloh extract combined with 250mg/L neem extract. Anti-stress commercial and extract treatment dissolved in drinking water. The treatment start from 08.00 until 18.00 for ten days. Next in the 11th day (chicken 25 days old)was inoculated Eimeria tanella sporulatif as much as 1 x 104 ookista/ml orally. Then the blood was taken sampling when the chicken 30 days old (five days after infected). Blood sampling was done in the brachial vein. Furthermore, the number of leukocytes and differential leukocyte.Was calculated data were analyzed using complete randomized design with the help of SPSS for Windows 1.8 program. The result of this research showed that giving jaloh and neem leaf extract were not giving significant effect (P0,05) towards the number of leukocyte as well as differential leukocyte of broilers that was infected with Eimeria tenella. The conclusion of this research were giving jaloh leaf extract and neem for 10 days was not giving significant effect towards number of leukocytes and differential leukocyte broilers that infected with Eimeria tenella