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All Journal Rotasi J@TI (TEKNIK INDUSTRI) Jurnal Ilmu Lingkungan Industrial Engineering Online Journal Prosiding Seminar Nasional Sains Dan Teknologi Fakultas Teknik Sinergi Jurnal Ilmiah Teknik Industri MIX : Jurnal Ilmiah Manajemen Jurnal Sistem dan Manajemen Industri Jurnal Teknik Industri: Jurnal Keilmuan dan Aplikasi Teknik Industri Indonesian Food Science and TechnologyJournal ComTech: Computer, Mathematics and Engineering Applications STI Policy and Management Journal Jurnal Pasopati : Pengabdian Masyarakat dan Inovasi Pengembangan Teknologi Advance Sustainable Science, Engineering and Technology (ASSET) Food Scientia: Journal of Food Science and Technology Makara Journal of Technology Jurnal Agrifoodtech Journal Community Service Consortium Jurnal Profesi Insinyur Indonesia
Ratna Purwaningsih
Departemen Teknik Industri Fakultas Teknik - Universitas Diponegoro
Aerospace Engineering Agriculture, Biological Sciences & Forestry Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Electrical & Electronics Engineering Energy Engineering Environmental Science Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mathematics Mechanical Engineering Social Sciences Transportation

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Journal : Food Scientia: Journal of Food Science and Technology

 1 
Deteksi DNA Spesies pada Produk Makanan Olahan Daging Ayam Menggunakan Gen GH (Growth Hormone) sebagai Penanda Molekuler Sophian, Alfi; Utaminingsih, Sri; Yenita, Yenita; Purwaningsih, Ratna
Food Scientia : Journal of Food Science and Technology Vol 4 No 1 (2024): January - June
Publisher : LPPM Universitas Terbuka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33830/fsj.v4i1.6358.2024

Abstract

The species DNA detection test in processed chicken meat food products using genetic growth hormone (GH) gene markers is a novel approach for authenticity test regarding the presence of a particular species in a product. This research aimed to test the ability of the GH genetic marker to detect the presence of chicken species in processed chicken meat food products. The method used in this research is a molecular biology method using real-time PCR with the SYBR Green kit. The genetic marker primers used were designed independently from the NCBI website. Based on DNA isolation data, the DNA concentration value is 43.60 ng/µL – 45.20 ng/µL with an average of 44.11. The purity value of isolated DNA is 1.855 – 1.925 with an average of 1.906. Chicken species DNA was detected in all samples using real-time PCR analysis, with a Ct value of 24.50 and a Tm value of 78.10. However, the Ct and Tm values were not detected in the negative control (NTC) and specificity (internal) control samples. In the positive control LOD, the Ct value was detected at 27.20 with a Tm value of 79.40. In the positive control, a Ct value of 21.10 and a Tm value of 78.10 were detected. The results indicate that all samples contained the GH gene, and hence, chicken species DNA was detected in all samples. This suggests that the GH genetic marker can be potentially used for species DNA identification testing.
Co-Authors Adrian Hartanto, Adrian Afifah Ahmad Afif Aisyah Aisyah Aisyah Aisyah Alya Az Zahraa Aminatuzzuhra Aminatuzzuhra, Aminatuzzuhra Anam, Muhammad Syaiful Arief Sugiyanto Aries Susanty Arimi, Izzan Ary Arvianto Aulia, Yussy Ayu, Fadila Dyah Bambang Purwanggono Belan Adison Cahya Farradita Sulistya Cahyantari, Anggaina Elfandora Christine Nauli Ibrahim Della Dias Oktarianingrum Devi Amalia Ayuningtias Dien, Shane Atsya Amara Dinar Anggita Restuti Dyah Hesti Wardhani Dyah Priamsari Dzulfikar, Rifki Daris Ernan Rustiadi Erwin Ardiansyah Fadhlandi Naufan Zharfano Fadli, Dinda Anggraini Fahira, Ayu Leuditya Fauzi, Muhammad Yusufa Riyan Gita Mahdiah Gunawan, Mega Sri Handy Nugroho, Handy Hapsari, Chaterine Alvina Prima Hari Kuncara Haryo Santosa Hayyina, Hilma Heru Prastawa Hery Suliantoro Ikhsan Yenifi Khalis Riyangga Kharisma Panca Kurniawati, Kharisma Panca Kresnanggara, Dionisius Yudhit Kusumawaty, Rini Mahardhika Cakra Yudha Maulana Ihsan Wardhana Maypemi, Niari Prittu Meila Zulhiana Meriska Damayanti Muhammad Arif Widyoadi Nadira Apsari Naniek Utami H Naniek Utami Handayani Naniek Utami Handayani Novie Susanto NURUL MIRANDA, NURUL Oktaviani Hadiati Putri Oryzanti, Parwa P., Dyah Ayu Pajar Damar Kusuma Pangestu, Winda Widya Prayogi, M. Agam Prima Hazairin Pugara, Ade Purnawan Adi Wicaksono Putri Indah Ramadani Putri, Ade Aisyah Arifna Raharjo, Danu Raka Mahendra Sulistiyo Rani Rumita Restuti, Dinar Anggita Riko Agisdihan Afifi Riska Ayu Purnamasari Riza, Fhariq Aulia Rizki Ridha Illahi Setiawan, Marwan Shintyastuti, Annisa Rahma Silviana Silviana Simanjuntak, Christoper Frans Singgih Saptadi Sjarief Widjaja Sonia, Regita Sophian, Alfi Sri Gunani Partiwi Sri Hartini Sri Sumiyati Sri Utaminingsih Suci Permata Sari Sulardjaka Sulardjaka Susatyo Nugroho Susatyo Nugroho W.P. Susatyo Nugroho WP Syamsinuha, Nabiqa Theresia Sihombing Tri Listyorini Unggul Dewandana Erich Bagaskara, Unggul Dewandana Erich Utami, Ajeng Sri Vita Shany Nugrahaeni Wardah Wardah Wibowo, Wido Kusumo Widharto, Yusuf Wiguna, I Kadek Candra Parmana Wijanarko, Aji Wiwik Budiawan Yahya, Muhammad Dimas Yenifi, Ikhsan Yenita . Yudha, Mahardhika Cakra Yulfin, Muhammad Dhani Yulita Arni Priastiwi Yussy Aulia Zainal Fanani R Zainal Fanani Rosyada