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All Journal Jurnal Akuakultur Indonesia AQUATIC SCIENCE & MANAGEMENT e-Journal BUDIDAYA PERAIRAN JURNAL ILMIAH PLATAX JURNAL PERIKANAN DAN KELAUTAN TROPIS Insan Cita Jurnal Pengabdian Kepada Masyarakat
Henky Manoppo
Bagian Klinik Hewan, Fakultas Kedokteran Hewan, Universitas Udayana, Bali
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Environmental Science Public Health Social Sciences

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Journal : JURNAL ILMIAH PLATAX

 1 
Isolation and Antibacterial Activity Test of Seagrass Epiphytic Symbiont Bacteria Thalassia hemprichii from Bahowo Waters, North Sulawesi Maarisit, Ismariani; Angkouw, Esther D.; Mangindaan, Remy E. P.; Rumampuk, Natalie D. C.; Manoppo, Henky; Ginting, Elvy Like
Jurnal Ilmiah Platax Vol. 9 No. 1 (2021): ISSUE JANUARY - JUNE 2021
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.9.1.2021.34320

Abstract

Seagrass is a higher plant and has the ability to produce bioactive compounds such as antibacterial. Seagrass is also a host to a variety of bacteria. Bacteria that live in the host will produce the same compounds as the host's body. The utilization of symbiotic bacteria with seagrasses as producers of bioactive compounds such as antibacterial can be used as a solution to reduce excessive seagrass uptake in nature. On the other hand, bacteria have the advantage of being fast and easy to grow and can be mass-produced and more economical. This study aims to isolate and test the antibacterial activity of the epiphytic bacteria of seagrass symbionts. Epiphytic bacteria of seagrass symbionts were grown on Nutrient Agar media directly in the field and bacterial isolation was carried out based on the morphological characteristics of the bacterial isolates. The antibacterial activity test was carried out using the disc method with the test bacteria Stapylococcus aureus, Streptococcus mutans, Escherichia coli, Salmonella thypi, and antibiotics as positive controls. The ability of bacteria to produce antibacterial was indicated by the formation of an inhibition zone around the paper disc containing the epiphytic bacteria of the seagrass symbiont T. hemprichii. A total of 3 isolates of epiphytic bacteria were isolated from T. hemprichii seagrass from Bahowo Waters, Tongkaina Village, Bunaken District, these isolates are namely Epifit 1, Epiphyte 2, and Epiphyte 3. Epiphyte 2 isolate had antibacterial activity against S. mutans, S. aureus, and S. thypi test bacteria, Epiphyte 3 isolate had antibacterial activity against S. mutans, and S. thypi test bacteria.Key words: Bacteria; Antibacterial; T. hemprichii; symbionts; BahowoAbstrakLamun merupakan tumbuhan tingkat tinggi dan memiliki kemampuan menghasilkan senyawa  bioaktif seperti antibakteri. Lamun juga merupakan tempat hidup atau inang dari berbagai bakteri. Bakteri yang hidup pada inang akan menghasilkan senyawa yang sama dengan tubuh inangnya. Pemanfaatan bakteri yang bersimbiosis dengan lamun sebagai produsen senyawa bioaktif seperti antibakteri dapat dijadikan sebagai solusi dalam mengurangi pengambilan lamun yang berlebihan di alam. Dilain pihak, bakteri memiliki keunggulan karena pertumbuhan bakteri yang cepat dan mudah tumbuh, dapat diproduksi secara massal dan lebih ekonomis. Penelitian ini bertujuan untuk mengisolasi dan menguji aktivitas antibakteri dari bakteri epifit simbion lamun T. hemprichii dari Perairan Bahowo.  Bakteri epifit simbion lamun ditumbuhkan pada media NA secara langsung di lapangan dan isolasi bakteri dilaksanakan berdasarkan karateristik morfologi isolat bakteri. Uji aktivitas bakteri dilakukan menggunakan metode cakram dengan bakteri uji S. aureus, S. mutans, E. coli, dan S. thypi dan antibiotik sebagai kontrol positif. Kemampuan bakteri menghasilkan antibakteri ditandai dengan terbentuknya zona hambat disekitar kertas cakram yang mengandung bakkteri epifit simbion lamun T. hemprichii.  Sebanyak 3 isolat bakteri epifit berhasil diisolasi pada lamun T. hemprichii dari Perairan Bahowo, Kelurahan Tongkaina, Kecamatan Bunaken yaitu Epifit 1, Epifit 2, dan Epifit 3. Isolat epifit 3 memiliki aktivitas antibakteri terhadap bakteri uji S. thypi, isolat Epifit 2 terhadap bakteri uji S. mutans, S. aureus, dan S. thypi, isolat Epifit 3 terhadap bakteri uji S. mutans, dan S. thypi.Kata kunci: Bakteri; Antibakteri; T. hemprichii; Simbion; Bahowo
Analysis of Carotenoid Pigment Types in the Carapace of the Male Crab Grapsus albolinetaus Latreille in Milbert 1812 Mokoginta, Fatika Sari; Paransa, Darus Saadah Johanis; Kemer, Kurnati; Paulus, James J. H.; Kawung, Nickson J.; Manoppo, Henky
Jurnal Ilmiah Platax Vol. 9 No. 1 (2021): ISSUE JANUARY - JUNE 2021
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.9.1.2021.34421

Abstract

Carotenoid pigments have various colors such as yellow, orange, or red-orange. One of the carotenoid pigment sources is Crab G. albolineatus Latreille in Milbert 1812. Column chromatography separation technique was used to determine the metabolism of carotenoid pigments in the crab G. albolineatus latreille in Milbert 1812. This CC separation used hexane and acetone as the developer solution (70:30). The developer solution as known as the mobile phase is semipolar, while the stationary phase is silica powder G60. Therefore, it formed two metabolic pathways. The male G. albolineatus crab used in this study was on the D3 molting stage which had a concentration of 36.37 g/g dry residue and 4.72 g content. The types of pigments identified are: β – karoten, Zeaxanthin, lutein, β – kriptoxanthin dan Astaxanthin.Keywords: Carotenoid Pigments; G. albolineatus; Column Chromatography; MoltingAbstrakPigmen karotenoid memiliki berbagai warna seperti kuning, oranye, atau merah oranye. Salah satu sumber pigmen karotenoid adalah pada karapas kepiting G. albolineatus Latreille in Milbert 1812. Untuk mengetahui metabolisme jenis pigmen karotenoid pada kepiting Grapsus albolineatus latreille in Milbert 1812 yaitu menggunakan pemisahan kromatografi Kolom. Pemisahan KK ini menggunakan larutan pengembang heksan dan aseton (70:30). Larutan pengembang merupakan fase gerak yang bersifat semipolar dan fase diamnya menggunakan bubuk silika G60. Terbentuk dua  jalur metabolisme. Kepiting G. albolineatus jantan yang digunakan pada penelitian berada di stadium molting D3 dengan konsentrasi sebesar 36,37 µg/g berat residu kering dan kandungan 4,72 µg. Jenis pigmen yang teridentifikasi yaitu : β – karoten, Zeaxanthin, lutein, β – kriptoxanthin dan Astaxanthin.Kata kunci: Pigmen; Karotenoid; G. albolineatus; Kromatofrafi Kolom; Molting
Co-Authors Achmad A Bugis Adnan Wantasen Angkouw, Esther D. Ayu Maleko Bella, Sintia S. Clara Nunia Payung Cyska Lumenta Daniel Djokosetiyanto Darus S. Paransa Edwin D Ngangi Enang Harris Erly Y. Kaligis, Erly Y. Gercyana Marentek Ginting, Elvi L. Ginting, Elvie L. Ginting, Elvy Like Grace O. Tambani Hengky J Sinjal Hengky J. Sinjal, Hengky J. Henneke Pangkey Hutagaol, Anggun F. Jetti Treslah Saselah Joppy Mudeng Karisoh, Yuriani S. Kedis Lengka Kemer, Kurnati Kevin Octavian Kusen Kreckhoff, Reni L. Lukas Belseran Maarisit, Ismariani Magdalena E.F Kolopita Magdalena E.F. Kolopita Mantiri, Desy M. H Maweikere, Fabiola C. Meilina E Rawung Mochamad Fatuchri Sukadi Mokoginta, Fatika Sari Mokolensang, Jeffrie F. Monijung, Revol D. Mudeng, Cindy C. Natalie D Rumampuk Nickson J. Kawung, Nickson J. Novitarizky, Intan A. Pangalila, Novelia M.A. Pangemanan, Novie P.L. Pangkey, Hanneke Paulus, James J. H. Ratusmanga, Jihan Reiny A Tumbol Reiny A. Tumbol Remy E. P Mangindaan Sambuaga, Mutiara E. Sammy N.J Longdong Sammy N.J. Longdong Sartje Lantu Simanjuntak, Erika E. L. Sinubu, Wulan V. Sukenda . Suria Darwisito, Suria Suzanne L Undap Takou, Putri D. Umasugi, Aisin Undi, Chintia S. Usy N Manurung Veibe Warouw Wantah, Monika M. Yanuar, Aditya P.