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Potensi Keterlibatan Multipihak dan Pendampingan Perguruan Tinggi berkelanjutan bagi Pengembangan Ekowisata Kapalo Banda dengan Daya tarik Utama Pembudidayaan Lebah Tanpa Sengat (Apidae: Meliponinae). Henny Herwina; Jasmi; Dahelmi; Mansyurdin; Syamsuardi; Jabang Nurdin; Nofrita; Muhammad Nazri Janra; Rita Maliza; Eli Ratni; Muhammad Idris; Putra Santoso; Miftahul Ilmi; Nabilah Rahmachila Azura; Syakira Tiara Rezvi; Elni Fatimah; Fajri Hidayat; Jefrial Santoso
Warta Pengabdian Andalas Vol 32 No 1 (2025)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat (LPPM) Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jwa.32.1.108-119.2025

Abstract

The development of ecotourism in Kapalo Banda, Limau Manis, Padang, has potential to be expanded into agro-ecotourism through multi-stakeholder involvement. Key attractions include the scenic of river flows, agricultural cultivation, and stingless bee keeping, which support environmental sustainability and the local economy. With high vegetation diversity, the potential for stingless bee (galo-galo) breeding as a creative economic initiative offers new opportunities. This program aligns with the West Sumatra Provincial Government’s efforts to establish the area as a honey production center. However, challenges such as low colony production due to limited knowledge of farming techniques and the availability of flowering feed hinder its development. Through community engagement, empowerment activities were conducted from June to September 2024, including socialization, demonstrations, and training in bee farming techniques, supported by expert teams. Education focused on the potential of galo-galo and marketing strategies for local products. The results of this program indicate improved community skills and knowledge, supporting local economic sustainability and biodiversity conservation. Kapalo Banda partners have the potential to serve as a model for community empowerment based on natural resources, effectively enhancing community welfare. Multi-stakeholder involvement in developing Kapalo Banda Ecotourism has resulted in a series of activities ranging from cultivation preparation, colony propagation techniques, harvest preparation, to downstream processing and product marketing.
Monitoring Ekspresi Gen Chalcone Synthase dan Respon Pertumbuhan Lobak Singgalang (Brassica oleracea L.) Akibat Paparan Ultraviolet-B Ekstrem Amar, Puti Khairunnajwa; Mansyurdin, Mansyurdin; Syafni, Nova; Syafia, Ersa Nur; Idris, Muhammad
Vegetalika Vol 14, No 2 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/veg.103190

Abstract

Singgalang cabbage (Brassica oleracea L.) is a local vegetable native to West Sumatra, cultivated in highlands around Mount Singgalang, Tanah Datar. Vegetables from Brassica genus are recognized for their high nutritional value and potential as functional foods. Key secondary metabolites in Brassica species, i.e., phenolic compounds and their derivatives, play a crucial role in antioxidant activity and are essential in promoting health. Light exposure, particularly ultraviolet-B (UV-B, 280-315 nm), can enhance biosynthesis of these compounds. UV-B intensity affects various process in plants including the phenylpropanoid pathway involved in secondary metabolite production. This study aimed to assess the expression of the CHALCONE SYNTHASE (CHS) gene under different UV-B intensities (0.3–3.0 µmol·m-2·s-1, 4 h) and examine the effects of two extreme UV-B intensities (0.3 and 3.0 µmol·m-2·s-1, 4h.d-1) in a controlled environment for 14 days. The results showed that increasing UV-B intensity enhanced CHS expression (1.0 and 3.0 µmol·m-2·s-1 showed thicker bands compared to 0.3 µmol·m-2·s-1, with a faint band in the control). Extreme UV-B exposure reduced chlorophyll content by 35–37% compared to the control, while carotenoids remained unaffected. Anthocyanin accumulation increased under low-intensity UV-B, whereas flavonoid levels were higher under high-intensity UV-B, suggesting different functional roles. UV-B exposure also influenced stomatal number and density in leaf. This preliminary study highlights the significant role of UV-B in enhancing specific metabolites in Singgalang cabbage, supporting its potential as a functional food.
Pengembangan Objek Esgp Dengan Ikan Koi Di Kelurahan Lambung Bukit Kecamatan Pauh Kota Padang Mansyurdin, Mansyurdin; Asful, Ferdhinal; Zaini, Zaini; Novarino, Wilson; Herwina, Henny; Efrizal, Efrizal; Nofrita, Nofrita; Syaifullah, Syaifullah; Santoso, Putra; Maliza, Rita; Ridho, Muhammad Syifa'ur; Aszareta, Muhammad Andoni; Pratama, Raihan Anugrah; Putri, Syntia Mai; Aziz, M. Abdul
Jurnal Pengabdian kepada Masyarakat Nusantara Vol. 6 No. 2 (2025): Jurnal Pengabdian kepada Masyarakat Nusantara Edisi April - Juni
Publisher : Lembaga Dongan Dosen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55338/jpkmn.v6i2.5872

Abstract

Keterbatasan objek dan prasarana penunjang wisata alam di ESGP belum memberikan dampak peningkatkan perekonomian bagi anggota P4S Sunkai Permai. Untuk mengatasi masalah ini telah dibuat kolam ikan koi yang alami untuk menambah daya tarik pengunjung. Rancangan kolam alami menggunakan material batu kali dengan tanaman bunga. Setelah persyaratan kualitas air terpenuhi, kolam diisi dengan 110 ikan koi dewasa dari 13 varietas. Pihak mitra P4S Sunkai Permai mampu merawat kolam dan menjaga kesehatan ikan, pemberian pakan dan menjinakan ikan koi sesuai dengan panduan yang diberikan. Pihak mitra juga memberikan pelayanan sesuai panduan edukasi yang mencakup sejarah dan asal usul, makna dan simbol memelihara ikan koi, biologi ikan koi, dan varietas ikan koi. Objek wisata ikan koi telah meningkatkan jumlah kunjungan ke ESGP dalam jangka waktu tiga bulan. Memperhatikan daya tarik objek ikan koi tersebut, direkomendasikan pengayaan objek dengan berbagai ikan hias lainnya di areal ESGP, yang pada akhirnya dapat lebih menigkatkan jumlah pengunjung.
Kloning dan karakterisasi gen penyandi inhibitor proteinase dari kulit buah kakao Cloning and characterization of gene encoding proteinase inhibitor of cacao pod wall Mayta Novaliza ISDA; Musliar KASIM; . MANSYURDIN; Tetty CHAIDAMSARI; Djoko SANTOSO
Menara Perkebunan Vol. 76 No. 2: 76 (2), 2008
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v76i2.84

Abstract

Summary Attempts to increase cocoa production in Indonesia have been hinderred by attack of CPB (Conopomorpha cramerella). There has been no effective measures to control this pest leading to development of cacao planting materials which resistant to the pod borer. One of genes functioning in plant defense system against insect pests such as catepilar is Proteinase Inhibitor (PIN). This research aimed to isolate and characterize TcPIN gene of cacao pod wall. A clone of TcPIN was isolated with RT-PCR technique using total RNA of cacao pod wall and DNA primer designed based on the sequence Trypsin Inhibitor of cocoa bean accessible online. BlastX analysis of the sequence of the cDNA clone demonstrated that the ± 600 bp gene cloned with pGEM-T was PIN gene as indicated by highly homologous to Trypsin Inhibitor of Theobroma microcarpum resulted in 248 Score bits and E value 1 e-64. Two sequence alligment with the putative 21 kDa PIN  of cacao seed indicated a moderately high homology. Contrasting these two sequences however found some non identical amino acids implying some variations. Ringkasan Usaha peningkatan produksi kakao di Indonesia terkendala antara lain oleh adanya serangan hama PBK (Conopomorpha cramerella). Untuk menanggulangi serangan PBK tersebut perlu adanya satu cara pengendalian yang efektif dan efisien, sehingga dapat mendorong usaha pengembangan bahan tanam yang tahan PBK. Salah satu gen  membawa sifat ketahanan tanaman terhadap hama ulat adalah Proteinase Inhibitor (PIN). Penelitian ini bertujuan untuk mengisolasi dan mengkarakterisasi gen TcPIN dari kulit buah kakao. Klon cDNA TcPIN diisolasi dari kulit buah kakao dengan teknik RT-PCR meng-gunakan RNA total kulit buah kakao dan primer DNA yang dirancang atas dasar sekuen Inhibitor Tripsin biji kakao yang diakses lewat internet.  Hasil analisis BlastX dari sekuen klon cDNA menunjukkan  bahwa gen berukuran  ± 600 pb yang telah diklon dengan pGEM-T tersebut adalah PIN karena memiliki homologi yang tinggi terhadap 21 kDa trypsin inhibitor dari Theobroma microcarpum yang meng-hasilkan Skor 248 bits dengan Nilai E 1e-64. Penjajaran dua sekuen dengan PIN putatif 21 kDa yang berasal dari biji kakao menunjuk-kan tingkat homologi yang tinggi, dengan perbedaan nyata sehingga dapat terlihat bahwa keduanya tidak identik.
Optimizing Soybean Chlorophyll Content Under Drought Stress: Unveiling the Potential of Biostimulants from Padina minor Yamada with Different Solvent Extraction De Yudanur, Parissa Anandita; Noli, Zozy Aneloi; Mansyurdin, Mansyurdin
Jurnal Biologi Tropis Vol. 24 No. 1 (2024): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i1.6568

Abstract

Biostimulants are natural compounds that can stimulate plant growth. Biostimulants from Padina minor contain secondary metabolites and growth-regulating substances needed in various plant growth metabolisms, including soybeans (Glycine max L.). Biostimulants also play a role in enhancing drought stress tolerance in plants. Several solvents are used to extract natural compounds found in P. minor. This study aims to investigate the effect of P. minor biostimulant extracted using various solvents on soybeans' chlorophyll content under different drought stress levels. The study used a completely randomized design (CRD) with two factors: a. Solvent (control, aquadest, methanol, and ethanol) and b. Soil field capacity (100%, 75%, 50%, and 25%). Applying P.minor as biostimulant extracted with methanol solvent showed higher average chlorophyll a, b, and total chlorophyll than other solvent types. Imposing stress up to 25% did not significantly affect soybean chlorophyll levels. However, the interaction between soil field capacity and P. minor extract from methanol solvent can trigger resilience response to drought conditions up to 25% soil field capacity and provide the highest average chlorophyll content compared to other treatments during the soybean vegetative period. Methanol is the best solvent for extracting P. minor as biostimulant and can provide the highest average chlorophyll content at 25% soil field capacity.
Effect of Crude Extracts Fern Leaves as Biostimulants on Biomass and Nodulation of Soybean (Glycine max L.) Dilla, Arfa Iskhia; Noli, Zozy Aneloi; Mansyurdin, Mansyurdin
Jurnal Biologi Tropis Vol. 24 No. 1b (2024): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i1b.8122

Abstract

Soybean (Glycine max) is a primary food commodity for the people in Indonesia, with most of its needs still dependent on imports. Efforts to increase local production require a sustainable strategy, including by use biostimulants. Natural biostimulants, such as fern leaf extract, is proven to be a solution in increasing the growth of plants. This study aims to evaluate the potential use of fern leaf extract as a biostimulant on biomass and the number of root nodules in soybean plants. The method used in this study was a factorial complete randomized design with 2 factors and 3 replications. Factor A is the source of crude extract of fern leaves, control, Gleichenia linearis, Diplazium esculentum, Nephrolepis exaltata, and Blechnum orientale. Factor B is the frequency of application extracts, namely 1 time and 2 times.The results showed that the application of Diplazium esculentum leaf extract with one application significantly increased the total number of root nodules, the number of effective root nodules and vegetative phase biomass. While in Nephrolepis exaltata with one time application can significantly increase the number of total root nodules and the number of effective root nodules. This increase is indicated by the content of bioactive compounds contained in the extract, so that it can increase the growth of soybean plants by stimulating the activity of soil microorganisms.