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All Journal Vegetalika Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Natur Indonesia Jurnal Perikanan dan Kelautan AL KAUNIYAH Jurnal Pembangunan dan Alam Lestari Jurnal Biologi Universitas Andalas SEMIRATA 2015 Buletin Kebun Raya Jurnal AGROSAINS dan TEKNOLOGI Jurnal Biologi Tropis Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) Eksakta : Berkala Ilmiah Bidang MIPA Menara Perkebunan Floribunda Jurnal Pembelajaran dan Biologi Nukleus Sainstek : Jurnal Sains dan Teknologi Jurnal Pengabdian kepada Masyarakat Nusantara Warta Pengabdian Andalas: Jurnal Ilmiah Pengembangan Dan Penerapan Ipteks Berkala Penelitian Hayati Makara Journal of Science

. Mansyurdin

Andalas University, Padang

Author-ID : 314434

Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Social Sciences Other

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Penggandaan Kromosom dan Pertumbuhan Somaklonal Andalas (Morus macroura Miq. var macroura) yang Diperlakukan dengan Kolkisin Anzharni Fajrina; M Idris; - Mansyurdin; Netty W Surya
Jurnal Biologi Universitas Andalas Vol 1, No 1 (2012)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.1.1.%p.2012

The aim of this research was to find the best concentration of colchicine and soaking time to induce tetraploid plants and to study the growth responses of Andalas somaclone. The colchicine concentrations consist of 0.05, 0.1; 0.15% with soaking time were 72 and 96 hours respectively. The results showed that the concentration of 0.05% colchicine with 96 hours soaking time able to induce highest percentage shoot of Andalas tetraploid (75%). The highest percentage of roots induction and the best timing of root formation were obtained at concentration of 0.05% colchicine with 96 hours soaking time.Keywords: Morus macroura Miq. var macroura,somaclone, growth, chromosomes, colchicine

Induksi PLB Anggrek Vanda sumatrana Schltr. Liar Pada Media MS dengan Penambahan BAP dan NAA serta Ploidisasi dengan Kolkisin Hanifah - Aini; Mansyurdin - Mansyurdin; Suwirmen - Suwirmen
Jurnal Biologi Universitas Andalas Vol 4, No 4 (2015)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.4.4.208-215.2015

The study about PLB induction of wild Vanda sumatrana Schltr. on MS media suplement with BAP and NAA and ploidisation by colchicine treatment was conducted from December 2014 until November 2015 at the Laboratory of Genetics and Cell Biology and Laboratory of Plant Physiology and Tissue Culture, Biology department, Faculty of Mathematic and Natural Science, Andalas University, Padang. The study aimed to 1) knowing the best concentration of 6-Benzyl amino purin (BAP) and α-Naphtalene acetic acid (NAA) for Protocorm Like Bodies (PLB) induction from shoot tip of V. sumatrana, 2) knowing the PLB response of V. sumatrana to concentrations and soak period of colchicine and 3) find the effective concentrations and soak period of colchicine to induce tetraploid on PLB of V. sumatrana. Shoot tips from in-vitro cultured of V. sumatrana were subcultured on Murashinge and Skoog (MS) medium supplement with 3 mg/l BAP + 0,5 mg/l NAA, 3 mg/l BAP and 1,5 mg/l BAP. PLB of diploid V. sumatrana from the best treatment were soaked in 0.05% and 0.1% colchicine for 24 and 48 hours respectively in MS liquid medium, as control were set PLB without colchicine treatment. The results showed that MS medium supplemented with 1.5 mg/l BAP was the best formula to induce PLB. The highest percentage of survival rate of PLB and percentage of survived PLB regenerated shoot was obtained from 0.05% colchicine with 24 hours soak period treatment. The effective treatment to induce tetraploid on PLB of V. sumatrana Schltr. was obtained from 0.05% colchicine solution for 24 hours soak period.

PERKEMBANGAN AERENKIM AKAR KANGKUNG DARAT (Ipomoea reptans Poir) DAN KANGKUNG AIR (Ipomoea aquatic Forsk) Aliya Ningsih; Mansyurdin Mansyurdin; Tesri Maideliza
Al-Kauniyah: Jurnal Biologi Vol 9, No 1 (2016): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Abstrak Penelitian tentang perkembangan aerenkim pada kangkung darat dan kangkung air telah dilakukan pada bulan Oktober 2014 sampai Februari 2015 di Laboratorium Struktur Perkembangan Tumbuhan Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Andalas. Jaringan akar diproses dengan metode parafin, kemudian dideskripsikan secara kuantitatif. Rongga aerenkim pada kangkung air terbentuk pada minggu ke tiga dengan jumlah dua rongga sel sedangkan pada kangung darat terbentuk pada minggu ke empat. Proses pembentukan aerenkim terjadi melalui proses pelisisan sel korteks.Abstract Research of aerenchyma development on terrestrial kale Ipomea reptans poir and water kale Ipomoea aquatic Forsk was conducted from October, 2014 until February, 2015 at Laboratory of plant growth structure, the Faculty of Math and Science, Department of Biology, Andalas University. Kales were analyzed by using paraffin method then described by quantitive data. Results showed: (i) aerenchyma cavities were formed in the 1st week, (ii) aquatic Forsks with two cell cavities were formed in the 3rd week, (iii) terrestrial kales were formed in the 4th week. The formation process of aerenchyma occurred through lysis process of cortical cell.

Deskripsi ikan pantau janggut, Esomus metallicus Ahl 1924 (Cyprinidae) dari anak Sungai Siak dan kanal-kanal di Provinsi Riau [Description of pantau janggut fish, Esomus metallicus Ahl 1924 (Cyprinidae) from tributaries of Siak River and canals in Riau Province] Chaidir P. Pulungan; Indra Junaidi Zakaria; Sukendi Sukendi; Mansyurdin Mansyurdin
Jurnal Iktiologi Indonesia Vol 11 No 2 (2011): Desember 2011
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v11i2.136

Striped flying barb (Esomus metallicus) is a freshwater fish that is belonged to family Cyprinidae and genus Esomus. This fish commonly occur in the tributaries of the Siak River branches, namely Tenayan River (Tenayan Raya District, Pekanbaru Regency) and Tapung Mati River (Bencah Kelubi Village, Tapung District, Kampar Regency), and also inhabit canals along the H.R. Subrantas street in Pekanbaru. E. metallicus is small sized fish (maximum 7.3 cm TL) and characterized by oblong and compressed body; mouth opening small, oblique and directed upward; having two pair barbels, rostral barbell reach the area behind the eye, while the maxillary barbel reach anal fin. In the lateral side of the body, there is a black band that is elongated from the upper operculum to the base of caudal fin. Dorsal and anal fins are short, they are located in the opposite side of the body, but first ray of the dorsal fin is slightly frontal than the first ray of the anal fin. Linea lateralis is incomplete. AbstrakPantau janggut (Esomus metallicus) adalah ikan air tawar yang tergolong ke dalam famili Cyprinidae dan genus Esomus. Ikan ini dijumpai di anaksungai-anaksungai Siak, yaitu: Sungai Tenayan (berada di wilayah Kecamatan, Tenayan Raya, Kota Pekanbaru) dan Sungai Tapung Mati (berada di wilayah Desa Bencah Kelubi, Kecamatan Tapung, Kabupa-ten Kampar) serta di parit-parit Kota Pekanbaru sekitar Jalan H.R. Soebrantas. Ikan pantau merupakan ikan berukuran kecil (panjang tubuh maksimum 7,3 cm), tubuhnya pipih memanjang, bukaan mulut kecil dengan posisi mulut menga-rah ke atas, memiliki dua pasang sungut (sungut rostral mencapai belakang bola mata dan sungut rahang atas mencapai permulaan dasar sirip anal), pada sisi lateral tubuh terdapat pola warna hitam berbentuk seperti pita memanjang mulai dari bagian atas tutup insang hingga ke pangkal sirip ekor. Sirip dorsal dan analnya berukuran pendek dengan posisi ke-dua sirip pada tubuh saling bertolak belakang, akan tetapi permulaan sirip dorsal sedikit di depan awal dasar sirip anal. Ikan ini memiliki gurat sisi tetapi tidak lengkap.

VARIASI MORFOLOGI POLEN GENUS GLOBBA (ZINGIBERACEAE) DI SUMATRA BARAT Syamsuardi; Mansyurdin; Nurainas; Tri Susanti
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 16 No 1 (2010): December 2010
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/286

Pollen morphology of nine taxa of Globba (Globba leucantha, G. patens, G. variabilis, G. hasseltii,, G. fecunda, G. atrosanguinea, G. aurantiaca, G. multifolia dan G. Paniculata) that collected from various fields in the regions of West Sumatra were examined. Four diagnostic characteristics (pollen types, shapes, apertures and ornamentations) were examined and photographed by scanning electron microscopes (SEM). There was variation of pollen characteristics between nine species of West Sumatran Globba. The pollen shape of five Globba species (G. leuchanta, G. fecunda, G. atrosanguinea) was oblate. The spheroidal shapes were detected at G. variabilis, G. hasseltii dan G. multifolia. The unique prolete shape only detected at Globba patens. For aperture characteristics, the existence aperture were detected at pollens of four species (G. leuchanta, G. patens, G. atrosanguinea and G. aurantiaca) but was not detected at pollens of G. variabilis, G. hasseltii G. fecunda, G. multifolia, and G. paniculata. Four types of exine ornamentation characters were detected namely: echinate-retikulate at G. leuchanta, G. fecunda, and G. atrosanguinea; echinate-granulate at G. patens, G. hasseltii, G. multifolia, G. aurantiaca, and unique echinate-perforate and echinate-psilate were detected at G. variabilis and G. paniculata, respectively. All nine West Sumatran Globba species have the spina with variation in length (1.17 to 2.10 μm). These palynological data appear to be informative and useful for distingusing among species of Globba and for elucidating among Globba species.

PERBANDINGAN KOMPONEN MINYAK ATSIRI ANTARA DAUN MUDA DAN DAUN DEWASA PADA HYPTIS SUAVEOLENS (L.)POIT Moralita Chatri; Mansyurdin Mansyurdin; Amri Bakhtiar; Perri Adnadi
EKSAKTA: Berkala Ilmiah Bidang MIPA Vol. 18 No. 02 (2017): Eksakta : Berkala Ilmiah Bidang MIPA (E-ISSN : 2549-7464)
Publisher : Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Negeri Padang, Indonesia

Hyptis suaveolens (L.) Poit is one of the species Lamiaceae family (Labiatae), is an aromatic plant and can produce essential oils. Essential oils of these plants can be utilized as antimicrobials. The chemical components contained in the essential oils in the same plant may vary as they are influenced by several factors, including the growth stage. Essential oils can be obtained by hydrodestilation and their components are analyzed using gas chromatography-mass spectrometry (GC-MS). The essential oil components are then compared between those found in young leaves and adult leaves. The results of analysis and identification showed that in young leaves there are 50 components while in adult leaves there are only 37 components. The main components of young leaves were ?-caryophyllene (34.65%), germacrene-D (10.32%), ?-bergamotene (6.56%), ?-copaene (5.94%) and rimuene (6.46%), Are ?-caryophyllene (23.53%), germacrene-D (9.52%), ?-elemente (6.53%) and rimuene (13.19%). The components of ?-bergamotene and ?-copaene are not found in adult leaves.

Analysis of Nepenthes Hybrids in Taratak Hill, Pesisir Selatan Sumatera Barat by RAPD Technique Weni Enjelina; Mansyurdin Mansyurdin; Tesri Meideliza
EKSAKTA: Berkala Ilmiah Bidang MIPA Vol. 19 No. 2 (2018): Eksakta : Berkala Ilmiah Bidang MIPA (E-ISSN : 2549-7464)
Publisher : Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Negeri Padang, Indonesia

The Study about Analysis of Nepenthes Hybrids in Taratak Hill, Pesisir Selatan Sumatera Barat by RAPD Technique had been conducted from May until November 2011. The study was done to prove natural hybrids of Nepenthes by RAPD technique and to determine the relationship of natural hybrids of Nepenthes with both parental. The research method is survey and observation. Isolation of DNA carried by CTAB method with modifications and amplification carried out by PCR. Hybrids and parental relationship was obtained from Jaccard similarity coefficient and displayed in a Dendogram (family tree) and PCO analysis to look genetic variation was processed with the program PAST 2.10. Proof of the natural hybrid N. ampullaria × N. mirabilis can be done by the RAPD technique using the primers OPA 15, OPK 16, OPK 19 and OPP 15. Natural hybrids between N. ampullaria × N. gracilis using the primers OPA 15, OPK 19 and OPP 15. The eighth of natural hybrids N. ampullaria × N. mirabilis were closer to N. mirabilis, while both natural hybrids N. ampullaria × N. gracilis closer to the N. Ampullaria.

Kloning dan karakterisasi gen penyandi inhibitor proteinase dari kulit buah kakao Cloning and characterization of gene encoding proteinase inhibitor of cacao pod wall Mayta Novaliza ISDA; Musliar KASIM; . MANSYURDIN; Tetty CHAIDAMSARI; Djoko SANTOSO
E-Journal Menara Perkebunan Vol 76, No 2: Desember 2008
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Summary Attempts to increase cocoa production in Indonesia have been hinderred by attack of CPB (Conopomorpha cramerella). There has been no effective measures to control this pest leading to development of cacao planting materials which resistant to the pod borer. One of genes functioning in plant defense system against insect pests such as catepilar is Proteinase Inhibitor (PIN). This research aimed to isolate and characterize TcPIN gene of cacao pod wall. A clone of TcPIN was isolated with RT-PCR technique using total RNA of cacao pod wall and DNA primer designed based on the sequence Trypsin Inhibitor of cocoa bean accessible online. BlastX analysis of the sequence of the cDNA clone demonstrated that the ± 600 bp gene cloned with pGEM-T was PIN gene as indicated by highly homologous to Trypsin Inhibitor of Theobroma microcarpum resulted in 248 Score bits and E value 1 e-64. Two sequence alligment with the putative 21 kDa PIN of cacao seed indicated a moderately high homology. Contrasting these two sequences however found some non identical amino acids implying some variations. Ringkasan Usaha peningkatan produksi kakao di Indonesia terkendala antara lain oleh adanya serangan hama PBK (Conopomorpha cramerella). Untuk menanggulangi serangan PBK tersebut perlu adanya satu cara pengendalian yang efektif dan efisien, sehingga dapat mendorong usaha pengembangan bahan tanam yang tahan PBK. Salah satu gen membawa sifat ketahanan tanaman terhadap hama ulat adalah Proteinase Inhibitor (PIN). Penelitian ini bertujuan untuk mengisolasi dan mengkarakterisasi gen TcPIN dari kulit buah kakao. Klon cDNA TcPIN diisolasi dari kulit buah kakao dengan teknik RT-PCR meng-gunakan RNA total kulit buah kakao dan primer DNA yang dirancang atas dasar sekuen Inhibitor Tripsin biji kakao yang diakses lewat internet. Hasil analisis BlastX dari sekuen klon cDNA menunjukkan bahwa gen berukuran ± 600 pb yang telah diklon dengan pGEM-T tersebut adalah PIN karena memiliki homologi yang tinggi terhadap 21 kDa trypsin inhibitor dari Theobroma microcarpum yang meng-hasilkan Skor 248 bits dengan Nilai E 1e-64. Penjajaran dua sekuen dengan PIN putatif 21 kDa yang berasal dari biji kakao menunjuk-kan tingkat homologi yang tinggi, dengan perbedaan nyata sehingga dapat terlihat bahwa keduanya tidak identik.

Soasialisasi SBMPTN dan Praktek Pembahasan Soal-Soal SBMPTN Bidang Biologi pada SMA N 15 Padang Mildawati Mildawati; Mansyurdin Mansyurdin; Ardinis Arbain; Jabang Nurdin; Solfiyeni Solfiyeni
Warta Pengabdian Andalas Vol 23 No 1 (2016): Warta Pengabdian Andalas
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat (LPPM) Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar

Salah satu cara bagi para pelajar kelas XII untuk menduduki bangku di perguruan tinggi adalah dengan mengikuti ujian masuk bersama yang dikenal dengan SBMPTN (Seleksi Bersama Masuk Perguruan Tinggi Negeri) yang diadakan serentak se-Indonesia. Tingginya tingkat persaingan yang dihadapi pelajar dan tingkat kesulitan soal yang cukup tinggi membuat para siswa sangat membutuhkan peran dari universitas dalam mengarahkan dan memperkenalkan langsung para siswa kelas XII terhadap pola-pola penerimaan mahasiswa baru. Berdasarkan realita tersebut maka para pelajar sangat memerlukan bimbingan dalam mengenal dan mempelajari dengan cara seksama soal-soal yang ada di SBMPTN. Metode yang digunakan pada kegiatan ini yaitu ceramah langsung dan praktek di dalam kelas tentang analisa dan bahasan dari soal-soal SBMPTN tersebut. Dari hasil penemuan dilapangan maka didapatkan antusiasme siswa yang sangat tinggi tentang keinginannya untuk mengetahui pola-pola penerimaan mahasiswa baru di perguruan tinggi. Hal ini terlihat dari banyaknya pertanyaan-pertanyaan yang mereka lemparkan dan rasa ingin tahu yang cukup tinggi tentang pola dan pembahasan dari soal-soal SBMPTN khususnya bidang Biologi.

ANALISIS MORFOMETRI DAN BIOLOGI REPRODUKSI ANAPHALIS JAVANICA DAN A.LONGIFOLIA (ASTERACEAE) DI SUMATERA BARAT Ahmad Taufiq; Syamsuardi Syamsuardi; Ardinis Arbain; Tesri Maideliza; Mansyurdin Mansyurdin
Floribunda Vol. 4 No. 7 (2013)
Publisher : PTTI

Anaphalis javanica and A. longifolia, were collected during the expeditionto Mt. Merapi, Singgalang, Tandikat, and Talang (West Sumatra). Beside two of these species, several individuals of Anaphalis that differs in several characters from the two others species were also found. Based on it, morphometric study and biology reproduction analysis were carried out to clarify their taxonomic classification. Fifty three individuals of Anaphalis were collected and twenty four characters had been observed and measured to define species and morphology variation. Data analysis revealed that the alien species were defined as a natural hybrid of A. javanica and A. longifolia. There was no significant difference that number and morphology of pollens among populations as well as those two species studied. Based on the P/O ratio analysis indicated that breeding system of A. javanica and A. longifolia were facultative xenogamy. The same ornamentation (echinate) in both of two species suggested that the natural hybridization between two species was possible.

Co-Authors ', Mardinus . Chairul . Yastori A.A. Ketut Agung Cahyawan W Ahmad Taufiq Aliya Ningsih Amanda Nurhafitri Amar, Puti Khairunnajwa Amri Bakhtiar Anthoni Agustien Antonie Agustien Anzharni Fajrina Ardinis Arbain Arifa Setriani Asful, Ferdhinal Ashrifurrahman, Ashrifurrahman Aszareta, Muhammad Andoni Aziz, M. Abdul Catur Hermanto Chaidir P. Pulungan Chaidir P. Pulungan Dahelmi Dahelmi De Yudanur, Parissa Anandita Dilla, Arfa Iskhia Djoko Santoso Djong Hon Tjong Efrizal Efrizal Eli Ratni Elisa, Tasya Putri Pratama Elni Fatimah Elza Safitri Ema Susiana Enjelina, Weni Erizal Muchktar Fajri Hidayat Fitri Handayani Gusmardi Indra Hanifah - Aini Hendra Kusuma Henny Herwina Herwita Idris Indra Junaidi Zakaria Jabang Nurdin Janra, Muhammad Nazri Jasmi Jefrial Santoso Joko Ridho Witono Joko Ridho Witono Joko Ridho Witono M. Idris Maliza, Rita Mayta Novaliza Isda Miftahul Ilmi Mildawati Mildawati Moralita Chatri Muhammad Idris Muhammad Idris Musliar Kasim Nabila, Sylvia Nabilah Rahmachila Azura Netty W Surya Nofrita Nofrita Nofrita Nofrita Nova Syafni Nurainas Nurainas Nurainas Nurmansyah Nurmansyah Perri Adnadi Pratama, Raihan Anugrah Putra Santoso Putri, Syntia Mai Revis Asra Revis Asra Revis Asra Ridho, Muhammad Syifa'ur Riki Rinaldi Rikinovtian Burlis Rudi Febriamansyah Sjahridal Dahlan Solfiyeni Solfiyeni Suci Rahma Nita Sukendi Sukendi Suwirmen, Suwirmen Syabila, Hutri Dinda Syafia, Ersa Nur Syaifullah Syaifullah Syakira Tiara Rezvi Syamsuardi Syamsuardi Tesri Maideliza Tesri Meideliza Tetty CHAIDAMSARI Tri Susanti TRIMURTI HABAZAR Wilson Novarino Yulia Sandri Yulmira Yanti Zaini Zaini Zozy Aneloi Noli

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