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All Journal Vegetalika METAMORFOSA Journal of Biological Sciences Jurnal Natur Indonesia Jurnal Perikanan dan Kelautan AL KAUNIYAH Jurnal Pembangunan dan Alam Lestari Jurnal Biologi Universitas Andalas SEMIRATA 2015 Buletin Kebun Raya Jurnal AGROSAINS dan TEKNOLOGI Jurnal Biologi Tropis Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) Eksakta : Berkala Ilmiah Bidang MIPA Menara Perkebunan Floribunda Sainstek : Jurnal Sains dan Teknologi Jurnal Pengabdian kepada Masyarakat Nusantara Warta Pengabdian Andalas: Jurnal Ilmiah Pengembangan Dan Penerapan Ipteks Berkala Penelitian Hayati Makara Journal of Science

. Mansyurdin

Andalas University, Padang

Author-ID : 314434

Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Social Sciences Other

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PERBANDINGAN KOMPONEN MINYAK ATSIRI ANTARA DAUN MUDA DAN DAUN DEWASA PADA HYPTIS SUAVEOLENS (L.)POIT Moralita Chatri; Mansyurdin Mansyurdin; Amri Bakhtiar; Perri Adnadi
EKSAKTA: Berkala Ilmiah Bidang MIPA Vol. 18 No. 02 (2017): Eksakta : Berkala Ilmiah Bidang MIPA (E-ISSN : 2549-7464)
Publisher : Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Negeri Padang, Indonesia

Hyptis suaveolens (L.) Poit is one of the species Lamiaceae family (Labiatae), is an aromatic plant and can produce essential oils. Essential oils of these plants can be utilized as antimicrobials. The chemical components contained in the essential oils in the same plant may vary as they are influenced by several factors, including the growth stage. Essential oils can be obtained by hydrodestilation and their components are analyzed using gas chromatography-mass spectrometry (GC-MS). The essential oil components are then compared between those found in young leaves and adult leaves. The results of analysis and identification showed that in young leaves there are 50 components while in adult leaves there are only 37 components. The main components of young leaves were ?-caryophyllene (34.65%), germacrene-D (10.32%), ?-bergamotene (6.56%), ?-copaene (5.94%) and rimuene (6.46%), Are ?-caryophyllene (23.53%), germacrene-D (9.52%), ?-elemente (6.53%) and rimuene (13.19%). The components of ?-bergamotene and ?-copaene are not found in adult leaves.

Analysis of Nepenthes Hybrids in Taratak Hill, Pesisir Selatan Sumatera Barat by RAPD Technique Weni Enjelina; Mansyurdin Mansyurdin; Tesri Meideliza
EKSAKTA: Berkala Ilmiah Bidang MIPA Vol. 19 No. 2 (2018): Eksakta : Berkala Ilmiah Bidang MIPA (E-ISSN : 2549-7464)
Publisher : Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Negeri Padang, Indonesia

The Study about Analysis of Nepenthes Hybrids in Taratak Hill, Pesisir Selatan Sumatera Barat by RAPD Technique had been conducted from May until November 2011. The study was done to prove natural hybrids of Nepenthes by RAPD technique and to determine the relationship of natural hybrids of Nepenthes with both parental. The research method is survey and observation. Isolation of DNA carried by CTAB method with modifications and amplification carried out by PCR. Hybrids and parental relationship was obtained from Jaccard similarity coefficient and displayed in a Dendogram (family tree) and PCO analysis to look genetic variation was processed with the program PAST 2.10. Proof of the natural hybrid N. ampullaria × N. mirabilis can be done by the RAPD technique using the primers OPA 15, OPK 16, OPK 19 and OPP 15. Natural hybrids between N. ampullaria × N. gracilis using the primers OPA 15, OPK 19 and OPP 15. The eighth of natural hybrids N. ampullaria × N. mirabilis were closer to N. mirabilis, while both natural hybrids N. ampullaria × N. gracilis closer to the N. Ampullaria.

Kloning dan karakterisasi gen penyandi inhibitor proteinase dari kulit buah kakao Cloning and characterization of gene encoding proteinase inhibitor of cacao pod wall Mayta Novaliza ISDA; Musliar KASIM; . MANSYURDIN; Tetty CHAIDAMSARI; Djoko SANTOSO
E-Journal Menara Perkebunan Vol 76, No 2: Desember 2008
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Summary Attempts to increase cocoa production in Indonesia have been hinderred by attack of CPB (Conopomorpha cramerella). There has been no effective measures to control this pest leading to development of cacao planting materials which resistant to the pod borer. One of genes functioning in plant defense system against insect pests such as catepilar is Proteinase Inhibitor (PIN). This research aimed to isolate and characterize TcPIN gene of cacao pod wall. A clone of TcPIN was isolated with RT-PCR technique using total RNA of cacao pod wall and DNA primer designed based on the sequence Trypsin Inhibitor of cocoa bean accessible online. BlastX analysis of the sequence of the cDNA clone demonstrated that the ± 600 bp gene cloned with pGEM-T was PIN gene as indicated by highly homologous to Trypsin Inhibitor of Theobroma microcarpum resulted in 248 Score bits and E value 1 e-64. Two sequence alligment with the putative 21 kDa PIN of cacao seed indicated a moderately high homology. Contrasting these two sequences however found some non identical amino acids implying some variations. Ringkasan Usaha peningkatan produksi kakao di Indonesia terkendala antara lain oleh adanya serangan hama PBK (Conopomorpha cramerella). Untuk menanggulangi serangan PBK tersebut perlu adanya satu cara pengendalian yang efektif dan efisien, sehingga dapat mendorong usaha pengembangan bahan tanam yang tahan PBK. Salah satu gen membawa sifat ketahanan tanaman terhadap hama ulat adalah Proteinase Inhibitor (PIN). Penelitian ini bertujuan untuk mengisolasi dan mengkarakterisasi gen TcPIN dari kulit buah kakao. Klon cDNA TcPIN diisolasi dari kulit buah kakao dengan teknik RT-PCR meng-gunakan RNA total kulit buah kakao dan primer DNA yang dirancang atas dasar sekuen Inhibitor Tripsin biji kakao yang diakses lewat internet. Hasil analisis BlastX dari sekuen klon cDNA menunjukkan bahwa gen berukuran ± 600 pb yang telah diklon dengan pGEM-T tersebut adalah PIN karena memiliki homologi yang tinggi terhadap 21 kDa trypsin inhibitor dari Theobroma microcarpum yang meng-hasilkan Skor 248 bits dengan Nilai E 1e-64. Penjajaran dua sekuen dengan PIN putatif 21 kDa yang berasal dari biji kakao menunjuk-kan tingkat homologi yang tinggi, dengan perbedaan nyata sehingga dapat terlihat bahwa keduanya tidak identik.

Soasialisasi SBMPTN dan Praktek Pembahasan Soal-Soal SBMPTN Bidang Biologi pada SMA N 15 Padang Mildawati Mildawati; Mansyurdin Mansyurdin; Ardinis Arbain; Jabang Nurdin; Solfiyeni Solfiyeni
Warta Pengabdian Andalas Vol 23 No 1 (2016): Warta Pengabdian Andalas
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat (LPPM) Universitas Andalas

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Salah satu cara bagi para pelajar kelas XII untuk menduduki bangku di perguruan tinggi adalah dengan mengikuti ujian masuk bersama yang dikenal dengan SBMPTN (Seleksi Bersama Masuk Perguruan Tinggi Negeri) yang diadakan serentak se-Indonesia. Tingginya tingkat persaingan yang dihadapi pelajar dan tingkat kesulitan soal yang cukup tinggi membuat para siswa sangat membutuhkan peran dari universitas dalam mengarahkan dan memperkenalkan langsung para siswa kelas XII terhadap pola-pola penerimaan mahasiswa baru. Berdasarkan realita tersebut maka para pelajar sangat memerlukan bimbingan dalam mengenal dan mempelajari dengan cara seksama soal-soal yang ada di SBMPTN. Metode yang digunakan pada kegiatan ini yaitu ceramah langsung dan praktek di dalam kelas tentang analisa dan bahasan dari soal-soal SBMPTN tersebut. Dari hasil penemuan dilapangan maka didapatkan antusiasme siswa yang sangat tinggi tentang keinginannya untuk mengetahui pola-pola penerimaan mahasiswa baru di perguruan tinggi. Hal ini terlihat dari banyaknya pertanyaan-pertanyaan yang mereka lemparkan dan rasa ingin tahu yang cukup tinggi tentang pola dan pembahasan dari soal-soal SBMPTN khususnya bidang Biologi.

ANALISIS MORFOMETRI DAN BIOLOGI REPRODUKSI ANAPHALIS JAVANICA DAN A.LONGIFOLIA (ASTERACEAE) DI SUMATERA BARAT Ahmad Taufiq; Syamsuardi Syamsuardi; Ardinis Arbain; Tesri Maideliza; Mansyurdin Mansyurdin
Floribunda Vol. 4 No. 7 (2013)
Publisher : PTTI

Anaphalis javanica and A. longifolia, were collected during the expeditionto Mt. Merapi, Singgalang, Tandikat, and Talang (West Sumatra). Beside two of these species, several individuals of Anaphalis that differs in several characters from the two others species were also found. Based on it, morphometric study and biology reproduction analysis were carried out to clarify their taxonomic classification. Fifty three individuals of Anaphalis were collected and twenty four characters had been observed and measured to define species and morphology variation. Data analysis revealed that the alien species were defined as a natural hybrid of A. javanica and A. longifolia. There was no significant difference that number and morphology of pollens among populations as well as those two species studied. Based on the P/O ratio analysis indicated that breeding system of A. javanica and A. longifolia were facultative xenogamy. The same ornamentation (echinate) in both of two species suggested that the natural hybridization between two species was possible.

KAJIAN SISTEM POLINASI DAEMONOROPS DRACO (WILLD.) BLUME Revis Asra; Syamsuardi Syamsuardi; Mansyurdin Mansyurdin; Joko Ridho Witono
Floribunda Vol. 4 No. 7 (2013)
Publisher : PTTI

The pollination study on Daemonorops draco (Wildd.) Blume has been conducted based on structure of inflorescence, pollen and ovul ration, and apomixis. The result of study present that inflorescence structure of D. draco was androdioecious. Based on the color of the inflorescence on anther and crown indicated that D. draco pollination systems supported by insects (Trigona spp.). Pollination systems of D. draco based on ratio pollen ovule is xenogami (outcrossing). Apomixis process proved that fruit of D. draco can be formed without convergence of gametes/males and females which have the character of apomixis.

Analysis of Putative Hybrid between Anaphalis longifolia and A.javanica (Asteraceae) in Mount Talang, West Sumatra based on Anatomical Characteristics Fajrina, Anzharni; Mansyurdin,; Syamsuardi,
Makara Journal of Science Vol. 21, No. 1
Publisher : UI Scholars Hub

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heputative natural hybrid between Anaphalis longifolia and Anaphalis javanica from Talang Mountain in West Sumatra has been discovered. The aim of this research is to analyze the anatomical characteristics of the leaf and stemto clarify the status of this putative natural hybrid. The anatomical characteristics of leaves and stem were analyzed by making cross sectionsand paradermal sectionsusing Johansen’s method. The results show seven characteristics of this putative natural hybrid (the stomata density, length, width, andlength/width ratio; the number of cortical cell layers, the range of cortex length, and the range of cortex width) that were close to A. longifolia, one characteristic that was close to A. javanica (the structure of palisade), one characteristic that was intermediate (the structure of spongy layer), and one characteristic that was a combination of both of them (the trichome morphotype). All of the foregoing characteristics can be used to support the identification of a natural hybrid of A. longifolia and A. javanica.

KERAGAMAN ALEL GADUNG LIAR (Dioscorea bulbifera L.) DI SUMATERA BARAT Maideliza, Tesri; Mansyurdin, Mansyurdin
Makara Journal of Science Vol. 11, No. 1
Publisher : UI Scholars Hub

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The variety of six enzymes locus on three populations of Dioscorea bulbifera was revealed using both starch and polyacrilamide gel electrophoresis. Present study showed three polymorphic loci has each 2 alleles with nine total numbers of alleles for all populations examined. The higher alleles variety detected within population (Hs=0,08) than among population (Dst=0,04). The high levels of gene flow (0.53) were due to low differentiation among population. This result supported low levels genetic variation among population examined. Alozime data revealed moderate differentiation genetically between Western and Eastern part population from Bukit Barisan edge.

Pengendalian Athelia rolfsii Penyebab Busuk Pangkal Batang Pada Kacang Tanah Arachis hypogea. L Dengan Fungisida Nabati Dan Agensia Hayati (Review) Herwita Idris; Anthoni Agustien; Mansyurdin Mansyurdin
Jurnal AGROSAINS dan TEKNOLOGI Vol 8, No 2 (2023)
Publisher : Fakultas Pertanian - UMJ

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24853/jat.8.2.87-93

Athelia rolfsii, Curzi adalah bentuk seksualitas dari spesies Sclerotium rolfsii, Sacc, salah satu spesies jamur patogen yang dapat menyebabkan beberapa penyakit mematikan pada tanaman seperti busuk batang, layu dan rebah kecambah. Jamur ini merupakan jamur tular tanah yang dapat bertahan lama dan membentuk sklerotia di dalam tanah. Biasanya serangan dari patogen A. rolfsii, Curzi, ini masyarakat petani cenderung mengendalikannya dengan pemakaian pestisida yang berbahan aktif kimia. Hasil monitoring pengendalian yang memakai fungisida sintetis dalam mengendalikan patogen menimbulkan dampak negatif, akibat dari residu pestisida pada produk pertanian, serta resistensi dan resurgensi penyakit. Oleh sebab itu perlu dicari pestisida sebagai pengendali yang tidak mempunyai dampak negatif. Diantaranya adalah pemakaian pestisida yang bahan aktif tanaman ataupun hayati. Dari hasil beberapa penelitian yang telah dilakukan, setiap bahan baku yang berbeda akan mempunyai daya hambat yang berbeda pula. Untuk itu akan diuraikan dibawah ini hasil-hasil penelitian sebagai pengendali A. rolfsii Cruzi dari golongan fungisida nabati dan agens hayati.ABSTRACTAthelia rolfsii, Curzi is a form of sexuality from the species Sclerotium rolfsii, Sacc, one of the pathogenic fungal species that can cause several deadly diseases in plants such as stem rot, wilting and drooping of sprouts. This fungus is a soil-borne fungus that can survive for a long time and form sclerotia in the soil. Usually the attack is from the pathogen A. rolfsii, Curzi, the farming community tends to control it by using pesticides with active chemical ingredients. The results of monitoring control using synthetic chemical fungicides in controlling pathogens have a negative impact, as a result of pesticide residues in agricultural products, as well as disease resistance and resurgence. Therefore it is necessary to look for pesticides as controllers that do not have a negative impact. Among them is the use of pesticides with active plant or biological ingredients. From the results of several studies that have been carried out, each different raw material will have a different inhibition power. For this reason, the results of research as controllers for A. rolfsii Cruzi will be described below from the class of botanical fungicides and biological agents.

Potensi Pestisida Minyak Atsiri Untuk Pengendalian Jamur Fusarium oxysporum Penyebab Penyakit Layu Tanaman Budidaya Nurmansyah Nurmansyah; Antonie Agustien; Mansyurdin Mansyurdin
Jurnal AGROSAINS dan TEKNOLOGI Vol 8, No 2 (2023)
Publisher : Fakultas Pertanian - UMJ

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24853/jat.8.2.94-103

Pemakaian pestisida sintetis untuk pengendalian hama dan penyakit tanaman sudah lama digunakan, dan diketahui sangat efektif, namun penggunaan secara terus menerus dan berulang ulang menyebabkan pencemaran lingkungan, toksisitas residu, resistensi patogen dan bahaya bagi pengguna. Berdasarkan hal tersebut maka penelitian pemanfaatan pestisida nabati perlu di intensifkan hingga didapatkan pestisida nabati yang handal yang efektifitasnya tidak beda dengan pestisida sintetis serta ramah lingkungan. Fusarium oxysporum merupakan salah satu patogen yang sangat sulit dikendalikan, merupakan patogen tular tanah yang mempunyai forma species yang sangat banyak, gejala serangan pada tanaman berupa layu dan busuk. Namanya tergatung inang yang diserangnya, diantaranya yang sangat merugikan petani adalah Fusarium oxysporum f. sp. cubense, penyebab penyakit layu fusarium tanaman pisang, kejadian penyakit mencapai 64,45% bahkan dapat memusnahkan perkebunan pisang Cavendish dalam waktu lima tahun. Fusarium oxysporum f.sp. capsici, menyerang tanaman cabai kegagalan panen hingga 50%, Fusarium oxysporum f.sp. lycopersici penyebab penyakit layu tanaman tomat serangan mencapai 50% dan Fusarium oxysporum f. sp. Zingiberi menyebabkan kehilangan hasil jahe mencapai 90%. Tulisan ini menyajikan beberapa pestisida nabati minyak atsiri yang telah diujikan terhadap jamur patogen Fusarium oxysporum penyebab penyakit layu dan busuk fusarium pada beberapa tanaman yang dibudidayakan, diharapkan pestisida nabati ini dapat mengurangi pemakaian pestisida sintetisABSTRACTThe use of synthetic pesticides to control pests and plant diseases has been used for a long time, and is known to be very effective, however, continuous and repeated use causes environmental pollution, residual toxicity, pathogen resistance and danger to users. Based on this, research on the development of the use of vegetable pesticides needs to be intensified in order to obtain reliable plant pesticides for controlling pests and plant diseases, which are not much different in effectiveness from synthetic pesticides and are environmentally friendly. Fusarium oxysporum is one of the pathogens that is very difficult to control, is a soil borne pathogen that has a very large number of species forms, with symptoms of attack on plants in the form of wilting and rot. Its name depends on the host it attacks, one of which is quite important and very detrimental to farmers is Fusarium oxysporum f. sp. cubense, Fusarium oxysporum f.sp. capsici, Fusarium oxysporum f.sp. lycopersici and Fusarium oxysporum f. sp. zingiberi. This article presents several essential oil vegetable pesticides that have been tested against the pathogenic fungus Fusarium oxysporum which causes fusarium wilt and rot in several cultivated plants. It is hoped that these vegetable pesticides can reduce the use of synthetic pesticides.

Co-Authors ', Mardinus . Chairul . Yastori A.A. Ketut Agung Cahyawan W Ahmad Taufiq Aliya Ningsih Amar, Puti Khairunnajwa Amri Bakhtiar Anthoni Agustien Antonie Agustien Anzharni Fajrina Ardinis Arbain Asful, Ferdhinal Aszareta, Muhammad Andoni Aziz, M. Abdul Catur Hermanto Chaidir P. Pulungan Chaidir P. Pulungan Dahelmi Dahelmi De Yudanur, Parissa Anandita Dilla, Arfa Iskhia Djoko Santoso Efrizal Efrizal Eli Ratni Elni Fatimah Elza Safitri Ema Susiana Enjelina, Weni Erizal Muchktar Fajri Hidayat Fitri Handayani Gusmardi Indra Hanifah - Aini Henny Herwina Herwita Idris Indra Junaidi Zakaria Jabang Nurdin Janra, Muhammad Nazri Jasmi Jefrial Santoso Joko Ridho Witono Joko Ridho Witono Joko Ridho Witono M. Idris Maliza, Rita Mayta Novaliza Isda Miftahul Ilmi Mildawati Mildawati Moralita Chatri Muhammad Idris Muhammad Idris Musliar Kasim Nabilah Rahmachila Azura Netty W Surya Nofrita Nofrita Nofrita Nofrita Nova Syafni Nurainas Nurainas Nurmansyah Nurmansyah Perri Adnadi Pratama, Raihan Anugrah Putra Santoso Putri, Syntia Mai Revis Asra Revis Asra Ridho, Muhammad Syifa'ur Riki Rinaldi Rikinovtian Burlis Rudi Febriamansyah Sjahridal Dahlan Solfiyeni Solfiyeni Suci Rahma Nita Sukendi Sukendi Suwirmen, Suwirmen Syafia, Ersa Nur Syaifullah Syaifullah Syakira Tiara Rezvi Syamsuardi Syamsuardi Tesri Maideliza Tesri Meideliza Tetty CHAIDAMSARI Tri Susanti TRIMURTI HABAZAR Wilson Novarino Yulia Sandri Yulmira Yanti Zaini Zaini Zozy Aneloi Noli

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