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KAJIAN SISTEM POLINASI DAEMONOROPS DRACO (WILLD.) BLUME Revis Asra; Syamsuardi Syamsuardi; Mansyurdin Mansyurdin; Joko Ridho Witono
Floribunda Vol. 4 No. 7 (2013)
Publisher : PTTI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (530.917 KB) | DOI: 10.32556/floribunda.v4i7.2013.111

Abstract

The pollination study on Daemonorops draco (Wildd.) Blume has been conducted based on structure of inflorescence, pollen and ovul ration, and apomixis. The result of study present that inflorescence structure of D. draco was androdioecious. Based on the color of the inflorescence on anther and crown indicated that D. draco pollination systems supported by insects (Trigona spp.). Pollination systems of D. draco based on ratio pollen ovule is xenogami (outcrossing). Apomixis process proved that fruit of D. draco can be formed without convergence of gametes/males and females which have the character of apomixis.
Analysis of Putative Hybrid between Anaphalis longifolia and A.javanica (Asteraceae) in Mount Talang, West Sumatra based on Anatomical Characteristics Fajrina, Anzharni; Mansyurdin,; Syamsuardi,
Makara Journal of Science Vol. 21, No. 1
Publisher : UI Scholars Hub

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Abstract

heputative natural hybrid between Anaphalis longifolia and Anaphalis javanica from Talang Mountain in West Sumatra has been discovered. The aim of this research is to analyze the anatomical characteristics of the leaf and stemto clarify the status of this putative natural hybrid. The anatomical characteristics of leaves and stem were analyzed by making cross sectionsand paradermal sectionsusing Johansen’s method. The results show seven characteristics of this putative natural hybrid (the stomata density, length, width, andlength/width ratio; the number of cortical cell layers, the range of cortex length, and the range of cortex width) that were close to A. longifolia, one characteristic that was close to A. javanica (the structure of palisade), one characteristic that was intermediate (the structure of spongy layer), and one characteristic that was a combination of both of them (the trichome morphotype). All of the foregoing characteristics can be used to support the identification of a natural hybrid of A. longifolia and A. javanica.
KERAGAMAN ALEL GADUNG LIAR (Dioscorea bulbifera L.) DI SUMATERA BARAT Maideliza, Tesri; Mansyurdin, Mansyurdin
Makara Journal of Science Vol. 11, No. 1
Publisher : UI Scholars Hub

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Abstract

The variety of six enzymes locus on three populations of Dioscorea bulbifera was revealed using both starch and polyacrilamide gel electrophoresis. Present study showed three polymorphic loci has each 2 alleles with nine total numbers of alleles for all populations examined. The higher alleles variety detected within population (Hs=0,08) than among population (Dst=0,04). The high levels of gene flow (0.53) were due to low differentiation among population. This result supported low levels genetic variation among population examined. Alozime data revealed moderate differentiation genetically between Western and Eastern part population from Bukit Barisan edge.
Pengendalian Athelia rolfsii Penyebab Busuk Pangkal Batang Pada Kacang Tanah Arachis hypogea. L Dengan Fungisida Nabati Dan Agensia Hayati (Review) Herwita Idris; Anthoni Agustien; Mansyurdin Mansyurdin
Jurnal AGROSAINS dan TEKNOLOGI Vol 8, No 2 (2023)
Publisher : Fakultas Pertanian - UMJ

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24853/jat.8.2.87-93

Abstract

Athelia rolfsii, Curzi adalah bentuk seksualitas dari spesies Sclerotium rolfsii, Sacc, salah satu spesies jamur patogen yang dapat menyebabkan beberapa penyakit mematikan pada tanaman seperti busuk batang, layu dan rebah kecambah. Jamur ini merupakan jamur tular tanah yang dapat bertahan lama dan membentuk sklerotia di dalam tanah. Biasanya serangan dari patogen A. rolfsii, Curzi, ini masyarakat petani cenderung mengendalikannya dengan pemakaian pestisida yang berbahan aktif kimia. Hasil monitoring pengendalian yang memakai fungisida sintetis dalam mengendalikan patogen menimbulkan dampak negatif, akibat dari residu pestisida pada produk pertanian, serta resistensi dan resurgensi penyakit. Oleh sebab itu perlu dicari pestisida sebagai pengendali yang tidak mempunyai dampak negatif. Diantaranya adalah pemakaian pestisida yang bahan aktif tanaman ataupun hayati. Dari hasil beberapa penelitian yang telah dilakukan, setiap bahan baku yang berbeda akan mempunyai daya hambat yang berbeda pula. Untuk itu akan diuraikan dibawah ini hasil-hasil penelitian sebagai pengendali A. rolfsii Cruzi dari golongan fungisida nabati dan agens hayati.ABSTRACTAthelia rolfsii, Curzi is a form of sexuality from the species Sclerotium rolfsii, Sacc, one of the pathogenic fungal species that can cause several deadly diseases in plants such as stem rot, wilting and drooping of sprouts. This fungus is a soil-borne fungus that can survive for a long time and form sclerotia in the soil. Usually the attack is from the pathogen A. rolfsii, Curzi, the farming community tends to control it by using pesticides with active chemical ingredients. The results of monitoring control using synthetic chemical fungicides in controlling pathogens have a negative impact, as a result of pesticide residues in agricultural products, as well as disease resistance and resurgence. Therefore it is necessary to look for pesticides as controllers that do not have a negative impact. Among them is the use of pesticides with active plant or biological ingredients. From the results of several studies that have been carried out, each different raw material will have a different inhibition power. For this reason, the results of research as controllers for A. rolfsii Cruzi will be described below from the class of botanical fungicides and biological agents.
Potensi Pestisida Minyak Atsiri Untuk Pengendalian Jamur Fusarium oxysporum Penyebab Penyakit Layu Tanaman Budidaya Nurmansyah Nurmansyah; Antonie Agustien; Mansyurdin Mansyurdin
Jurnal AGROSAINS dan TEKNOLOGI Vol 8, No 2 (2023)
Publisher : Fakultas Pertanian - UMJ

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24853/jat.8.2.94-103

Abstract

Pemakaian pestisida sintetis untuk pengendalian hama dan penyakit tanaman sudah lama digunakan, dan diketahui sangat efektif, namun penggunaan secara terus menerus dan berulang ulang menyebabkan pencemaran lingkungan, toksisitas residu, resistensi patogen dan bahaya bagi pengguna. Berdasarkan hal tersebut maka penelitian pemanfaatan pestisida nabati perlu di intensifkan hingga didapatkan pestisida nabati yang handal yang efektifitasnya tidak beda dengan pestisida sintetis serta ramah lingkungan. Fusarium oxysporum merupakan salah satu patogen yang sangat sulit dikendalikan, merupakan patogen tular tanah yang mempunyai forma species yang sangat banyak, gejala serangan pada tanaman berupa layu dan busuk. Namanya tergatung inang yang diserangnya, diantaranya yang sangat merugikan petani adalah Fusarium oxysporum f. sp. cubense, penyebab penyakit layu fusarium tanaman pisang, kejadian penyakit mencapai 64,45% bahkan dapat memusnahkan perkebunan pisang Cavendish dalam waktu lima tahun. Fusarium oxysporum f.sp. capsici, menyerang tanaman cabai kegagalan panen hingga 50%, Fusarium oxysporum f.sp. lycopersici penyebab penyakit layu tanaman tomat serangan mencapai 50% dan Fusarium oxysporum f. sp. Zingiberi menyebabkan kehilangan hasil jahe mencapai 90%. Tulisan ini menyajikan beberapa pestisida nabati minyak atsiri yang telah diujikan terhadap jamur patogen Fusarium oxysporum penyebab penyakit layu dan busuk fusarium pada beberapa tanaman yang dibudidayakan, diharapkan pestisida nabati ini dapat mengurangi pemakaian pestisida sintetisABSTRACTThe use of synthetic pesticides to control pests and plant diseases has been used for a long time, and is known to be very effective, however, continuous and repeated use causes environmental pollution, residual toxicity, pathogen resistance and danger to users. Based on this, research on the development of the use of vegetable pesticides needs to be intensified in order to obtain reliable plant pesticides for controlling pests and plant diseases, which are not much different in effectiveness from synthetic pesticides and are environmentally friendly. Fusarium oxysporum is one of the pathogens that is very difficult to control, is a soil borne pathogen that has a very large number of species forms, with symptoms of attack on plants in the form of wilting and rot. Its name depends on the host it attacks, one of which is quite important and very detrimental to farmers is Fusarium oxysporum f. sp. cubense, Fusarium oxysporum f.sp. capsici, Fusarium oxysporum f.sp. lycopersici and Fusarium oxysporum f. sp. zingiberi. This article presents several essential oil vegetable pesticides that have been tested against the pathogenic fungus Fusarium oxysporum which causes fusarium wilt and rot in several cultivated plants. It is hoped that these vegetable pesticides can reduce the use of synthetic pesticides.
Potensi Keterlibatan Multipihak dan Pendampingan Perguruan Tinggi berkelanjutan bagi Pengembangan Ekowisata Kapalo Banda dengan Daya tarik Utama Pembudidayaan Lebah Tanpa Sengat (Apidae: Meliponinae). Henny Herwina; Jasmi; Dahelmi; Mansyurdin; Syamsuardi; Jabang Nurdin; Nofrita; Muhammad Nazri Janra; Rita Maliza; Eli Ratni; Muhammad Idris; Putra Santoso; Miftahul Ilmi; Nabilah Rahmachila Azura; Syakira Tiara Rezvi; Elni Fatimah; Fajri Hidayat; Jefrial Santoso
Warta Pengabdian Andalas Vol 32 No 1 (2025)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat (LPPM) Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jwa.32.1.108-119.2025

Abstract

The development of ecotourism in Kapalo Banda, Limau Manis, Padang, has potential to be expanded into agro-ecotourism through multi-stakeholder involvement. Key attractions include the scenic of river flows, agricultural cultivation, and stingless bee keeping, which support environmental sustainability and the local economy. With high vegetation diversity, the potential for stingless bee (galo-galo) breeding as a creative economic initiative offers new opportunities. This program aligns with the West Sumatra Provincial Government’s efforts to establish the area as a honey production center. However, challenges such as low colony production due to limited knowledge of farming techniques and the availability of flowering feed hinder its development. Through community engagement, empowerment activities were conducted from June to September 2024, including socialization, demonstrations, and training in bee farming techniques, supported by expert teams. Education focused on the potential of galo-galo and marketing strategies for local products. The results of this program indicate improved community skills and knowledge, supporting local economic sustainability and biodiversity conservation. Kapalo Banda partners have the potential to serve as a model for community empowerment based on natural resources, effectively enhancing community welfare. Multi-stakeholder involvement in developing Kapalo Banda Ecotourism has resulted in a series of activities ranging from cultivation preparation, colony propagation techniques, harvest preparation, to downstream processing and product marketing.
Monitoring Ekspresi Gen Chalcone Synthase dan Respon Pertumbuhan Lobak Singgalang (Brassica oleracea L.) Akibat Paparan Ultraviolet-B Ekstrem Amar, Puti Khairunnajwa; Mansyurdin, Mansyurdin; Syafni, Nova; Syafia, Ersa Nur; Idris, Muhammad
Vegetalika Vol 14, No 2 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/veg.103190

Abstract

Singgalang cabbage (Brassica oleracea L.) is a local vegetable native to West Sumatra, cultivated in highlands around Mount Singgalang, Tanah Datar. Vegetables from Brassica genus are recognized for their high nutritional value and potential as functional foods. Key secondary metabolites in Brassica species, i.e., phenolic compounds and their derivatives, play a crucial role in antioxidant activity and are essential in promoting health. Light exposure, particularly ultraviolet-B (UV-B, 280-315 nm), can enhance biosynthesis of these compounds. UV-B intensity affects various process in plants including the phenylpropanoid pathway involved in secondary metabolite production. This study aimed to assess the expression of the CHALCONE SYNTHASE (CHS) gene under different UV-B intensities (0.3–3.0 µmol·m-2·s-1, 4 h) and examine the effects of two extreme UV-B intensities (0.3 and 3.0 µmol·m-2·s-1, 4h.d-1) in a controlled environment for 14 days. The results showed that increasing UV-B intensity enhanced CHS expression (1.0 and 3.0 µmol·m-2·s-1 showed thicker bands compared to 0.3 µmol·m-2·s-1, with a faint band in the control). Extreme UV-B exposure reduced chlorophyll content by 35–37% compared to the control, while carotenoids remained unaffected. Anthocyanin accumulation increased under low-intensity UV-B, whereas flavonoid levels were higher under high-intensity UV-B, suggesting different functional roles. UV-B exposure also influenced stomatal number and density in leaf. This preliminary study highlights the significant role of UV-B in enhancing specific metabolites in Singgalang cabbage, supporting its potential as a functional food.
Pengembangan Objek Esgp Dengan Ikan Koi Di Kelurahan Lambung Bukit Kecamatan Pauh Kota Padang Mansyurdin, Mansyurdin; Asful, Ferdhinal; Zaini, Zaini; Novarino, Wilson; Herwina, Henny; Efrizal, Efrizal; Nofrita, Nofrita; Syaifullah, Syaifullah; Santoso, Putra; Maliza, Rita; Ridho, Muhammad Syifa'ur; Aszareta, Muhammad Andoni; Pratama, Raihan Anugrah; Putri, Syntia Mai; Aziz, M. Abdul
Jurnal Pengabdian kepada Masyarakat Nusantara Vol. 6 No. 2 (2025): Jurnal Pengabdian kepada Masyarakat Nusantara Edisi April - Juni
Publisher : Lembaga Dongan Dosen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55338/jpkmn.v6i2.5872

Abstract

Keterbatasan objek dan prasarana penunjang wisata alam di ESGP belum memberikan dampak peningkatkan perekonomian bagi anggota P4S Sunkai Permai. Untuk mengatasi masalah ini telah dibuat kolam ikan koi yang alami untuk menambah daya tarik pengunjung. Rancangan kolam alami menggunakan material batu kali dengan tanaman bunga. Setelah persyaratan kualitas air terpenuhi, kolam diisi dengan 110 ikan koi dewasa dari 13 varietas. Pihak mitra P4S Sunkai Permai mampu merawat kolam dan menjaga kesehatan ikan, pemberian pakan dan menjinakan ikan koi sesuai dengan panduan yang diberikan. Pihak mitra juga memberikan pelayanan sesuai panduan edukasi yang mencakup sejarah dan asal usul, makna dan simbol memelihara ikan koi, biologi ikan koi, dan varietas ikan koi. Objek wisata ikan koi telah meningkatkan jumlah kunjungan ke ESGP dalam jangka waktu tiga bulan. Memperhatikan daya tarik objek ikan koi tersebut, direkomendasikan pengayaan objek dengan berbagai ikan hias lainnya di areal ESGP, yang pada akhirnya dapat lebih menigkatkan jumlah pengunjung.
Kloning dan karakterisasi gen penyandi inhibitor proteinase dari kulit buah kakao Cloning and characterization of gene encoding proteinase inhibitor of cacao pod wall Mayta Novaliza ISDA; Musliar KASIM; . MANSYURDIN; Tetty CHAIDAMSARI; Djoko SANTOSO
Menara Perkebunan Vol. 76 No. 2: 76 (2), 2008
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v76i2.84

Abstract

Summary Attempts to increase cocoa production in Indonesia have been hinderred by attack of CPB (Conopomorpha cramerella). There has been no effective measures to control this pest leading to development of cacao planting materials which resistant to the pod borer. One of genes functioning in plant defense system against insect pests such as catepilar is Proteinase Inhibitor (PIN). This research aimed to isolate and characterize TcPIN gene of cacao pod wall. A clone of TcPIN was isolated with RT-PCR technique using total RNA of cacao pod wall and DNA primer designed based on the sequence Trypsin Inhibitor of cocoa bean accessible online. BlastX analysis of the sequence of the cDNA clone demonstrated that the ± 600 bp gene cloned with pGEM-T was PIN gene as indicated by highly homologous to Trypsin Inhibitor of Theobroma microcarpum resulted in 248 Score bits and E value 1 e-64. Two sequence alligment with the putative 21 kDa PIN  of cacao seed indicated a moderately high homology. Contrasting these two sequences however found some non identical amino acids implying some variations. Ringkasan Usaha peningkatan produksi kakao di Indonesia terkendala antara lain oleh adanya serangan hama PBK (Conopomorpha cramerella). Untuk menanggulangi serangan PBK tersebut perlu adanya satu cara pengendalian yang efektif dan efisien, sehingga dapat mendorong usaha pengembangan bahan tanam yang tahan PBK. Salah satu gen  membawa sifat ketahanan tanaman terhadap hama ulat adalah Proteinase Inhibitor (PIN). Penelitian ini bertujuan untuk mengisolasi dan mengkarakterisasi gen TcPIN dari kulit buah kakao. Klon cDNA TcPIN diisolasi dari kulit buah kakao dengan teknik RT-PCR meng-gunakan RNA total kulit buah kakao dan primer DNA yang dirancang atas dasar sekuen Inhibitor Tripsin biji kakao yang diakses lewat internet.  Hasil analisis BlastX dari sekuen klon cDNA menunjukkan  bahwa gen berukuran  ± 600 pb yang telah diklon dengan pGEM-T tersebut adalah PIN karena memiliki homologi yang tinggi terhadap 21 kDa trypsin inhibitor dari Theobroma microcarpum yang meng-hasilkan Skor 248 bits dengan Nilai E 1e-64. Penjajaran dua sekuen dengan PIN putatif 21 kDa yang berasal dari biji kakao menunjuk-kan tingkat homologi yang tinggi, dengan perbedaan nyata sehingga dapat terlihat bahwa keduanya tidak identik.
Genetic Variation of Siam Orange (Citrus nobilis Lour.) in Several Sumatran Production Centers using RAPD Marker: Genetic Variation of Citrus nobilis Lour in Several Sumatran Production Centers Arifa Setriani; Mansyurdin; Nurainas
Journal of Tropical Life Science Vol. 15 No. 2
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/7mr9as38

Abstract

Siam orange (Citrus nobilis Lour.) is a key horticultural commodity in Indonesia, valued for its adaptability, potential to improve farmer welfare, and contribution to the national economy. In Sumatra, four main production centres are located in Berastagi (North Sumatra), Gunung Omeh and Pasaman (West Sumatra), and Bangkinang (Riau). However, the origin of seedlings from these centres remains unclear. Despite high productivity, inconsistencies in fruit quality and genetic diversity among production centres highlight the need for molecular-level analysis to ensure superior varieties and maintain market competitiveness. This study aimed to determine the intra- and interpopulation genetic variations of Siam orange in these four production centres using RAPD markers. DNA isolation followed the Doyle and Doyle (1987) method, and amplification used 12 primers, of which OPA03, OPA10, and OPA01 produced the highest polymorphism (95.25%). Intrapopulation genetic variation was relatively low (H = 0.1061–0.1919), with Berastagi showing the lowest (H = 0.1061) and Gunung Omeh the highest (H = 0.1919). Interpopulation genetic diversity was classified as high (DST = 0.0619), with GST = 0.2946, and gene flow was Nm = 1.1975. UPGMA cluster analysis revealed that Gunung Omeh and Berastagi had the most significant genetic distance (0.1622), while Gunung Omeh and Bangkinang were the closest (0.0614). Principal Coordinates Analysis (PCoA) indicated that Berastagi individuals were more clustered, whereas Gunung Omeh, Pasaman, and Bangkinang were more dispersed. These findings indicate that while genetic variation within populations is limited, genetic differentiation among populations is substantial, offering opportunities to select and combine diverse genetic sources. This diversity can be strategically exploited in breeding programs to develop superior cultivars, improve fruit quality uniformity, and strengthen the commercial competitiveness of Siam orange cultivation in Sumatra.
Co-Authors ', Mardinus . Chairul . Yastori A.A. Ketut Agung Cahyawan W Ahmad Taufiq Aliya Ningsih Amanda Nurhafitri Amar, Puti Khairunnajwa Amri Bakhtiar Anthoni Agustien Antonie Agustien Anzharni Fajrina Ardinis Arbain Arifa Setriani Asful, Ferdhinal Ashrifurrahman, Ashrifurrahman Aszareta, Muhammad Andoni Aziz, M. Abdul Catur Hermanto Chaidir P. Pulungan Chaidir P. Pulungan Dahelmi Dahelmi De Yudanur, Parissa Anandita Dilla, Arfa Iskhia Djoko Santoso Djong Hon Tjong Efrizal Efrizal Eli Ratni Elisa, Tasya Putri Pratama Elni Fatimah Elza Safitri Ema Susiana Enjelina, Weni Erizal Muchktar Fajri Hidayat Fitri Handayani Gusmardi Indra Hanifah - Aini Hendra Kusuma Henny Herwina Herwita Idris Indra Junaidi Zakaria Jabang Nurdin Janra, Muhammad Nazri Jasmi Jefrial Santoso Joko Ridho Witono Joko Ridho Witono Joko Ridho Witono M. Idris Maliza, Rita Mayta Novaliza Isda Miftahul Ilmi Mildawati Mildawati Moralita Chatri Muhammad Idris Muhammad Idris Musliar Kasim Nabila, Sylvia Nabilah Rahmachila Azura Netty W Surya Nofrita Nofrita Nofrita Nofrita Nova Syafni Nurainas Nurainas Nurainas Nurmansyah Nurmansyah Perri Adnadi Pratama, Raihan Anugrah Putra Santoso Putri, Syntia Mai Revis Asra Revis Asra Revis Asra Ridho, Muhammad Syifa'ur Riki Rinaldi Rikinovtian Burlis Rudi Febriamansyah Sjahridal Dahlan Solfiyeni Solfiyeni Suci Rahma Nita Sukendi Sukendi Suwirmen, Suwirmen Syabila, Hutri Dinda Syafia, Ersa Nur Syaifullah Syaifullah Syakira Tiara Rezvi Syamsuardi Syamsuardi Tesri Maideliza Tesri Meideliza Tetty CHAIDAMSARI Tri Susanti TRIMURTI HABAZAR Wilson Novarino Yulia Sandri Yulmira Yanti Zaini Zaini Zozy Aneloi Noli