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All Journal IAES International Journal of Artificial Intelligence (IJ-AI) IJCCS (Indonesian Journal of Computing and Cybernetics Systems) TEKNIK INFORMATIKA JURNAL SISTEM INFORMASI BISNIS Bulletin of Electrical Engineering and Informatics POSITIF Journal of Tropical Biodiversity and Biotechnology KLIK (Kumpulan jurnaL Ilmu Komputer) (e-Journal) JOIV : International Journal on Informatics Visualization Jurnal Komputasi Jurnal Teknoinfo Jurnal Tekno Kompak Indonesian Journal of Electrical Engineering and Computer Science Jurnal Pengabdian Kepada Masyarakat (JPKM) Tabikpun Journal of Applied Data Sciences Journal of Multidisciplinary Applied Natural Science Buguh: Jurnal Pengabdian Kepada Masyarakat Jurnal Pepadun AMMA : Jurnal Pengabdian Masyarakat Integra: Journal of Integrated Mathematics and Computer Science
Admi Syarif
Jurusan Ilmu Komputer, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Lampung
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Nursing Physics Social Sciences

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Journal : Journal of Multidisciplinary Applied Natural Science

 1 
Molecular Phylogeny of rDNA-ITS on Native Dendrobium in Lampung Mahfut, Mahfut; Syarif, Admi; Wahyuningsih, Sri
Journal of Multidisciplinary Applied Natural Science Vol. 5 No. 2 (2025): Journal of Multidisciplinary Applied Natural Science
Publisher : Pandawa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47352/jmans.2774-3047.263

Abstract

Dendrobium, a flagship collection of the Liwa Botanical Garden, is an endemic flora of the Southern Sumatra that requires preservation. One of the challenges in its conservation and potential development is the molecular identification. This molecular identification utilizes DNA barcoding with the rDNA-ITS marker as a practical, rapid, accurate, effective, and efficient alternative, complementing previous species-level identification results based on morphological characteristics. Amplification results from 5 selected samples showed specific bands measuring 300 bp. Sequence data analysis using BioEdit and MEGA V.11.0.11 software with 1000 bootstraps grouped all accessions into the same main cluster with a similarity range of 94–100%. Phylogenetic analysis revealed that accession D2 is similar to D. signatum from Japan (AB593662.1), and accession D3 to D. densiflorum (HQ114255), D4 to D. nobile (LC011413.1), accession D6 to D. trigonopus (KF143730.1), and accession D12 to D. faciferum (LC192955.1) from China. The results of this study will enrich the taxonomic and phylogenetic data of Dendrobium, which is essential for conservation and serves as a foundation for its development as a medicinal herbal plant.
Co-Authors Agus Wantoro Agus Wantoro Akmal Junaidi Ami Zuraida Andrian, Rico Anggi Puspitasari Anggun N. Azizah Ani Kurniawati Aqshal Dwi Setiawan Arafia Isnayu Akaf Ari Ardianto Arie Setya Putra Aristoteles, Aristoteles Ayu Nadila Ayu Sangging, Putu Ristyaning Bambang Hermanto Dedy Miswar Deswita Sari Dimas A. Dhafa Dwi Sakethi Erika Fadia Salsabila Fahurian, Fatimah Favorisen R. Lumbanraja Fazri, Yudistira Febi Eka Febriansyah Fitriyana, Silfia Ghraito Arip Heni Sulistiani Heningtyas, Yunda Iva Mutiara Indah Kendari, Putri Khairun Nisa Krisna Rendi Awalludin Kurnia Muludi Kurnia Muludi M Said Hasibuan M. Juandhika Rizky Machudor Yusman Mahfut Maya Asterita Mohammad Surya Akbar Muhammad Irfan Ardiansyah Muhammad Jamaludin Muhammad Reza Faisal, Muhammad Reza Muhammad Rizki Nabila Z. Muhammad Ni K. Aprilliani Nisa Berawi, Khairun Noverina Rahmaniyanti Novita Dwilestari Nur Indriani Prabowo, Rizky Prabowo, Rizky Putri Ayu Penita Qory Aprilarita Rahmat Safe’i Raras Silviana Redy Susanto, Erliyan Rossyking, Favorisen Shofi, Imam Marzuki Shofiana, Dewi Asiah Sintiya Paramitha Sri Ratna Sulistiyanti Sri Wahyuningsih Sri Wahyuningsih Sugaluh Yulianti Sumaryo Gitosaputro Susiyanti, Endah Sutyarso Sutyarso Syachrul Priyo Wibowo TANJUNG, AKBAR RISMAWAN Timotius Pascha Tristiyanto Tundjung Tripeni Handayani Warsito Warsito Wulansari, Ossy Endah Dwi Yarmaidi Yarmaidi Yoannisa Egeustin Yodhi Yuniarthe Yokie Rahman Yulia K. Wardani Yuliyanto, Kurniawan Dwi Zahra, Rizka Aulia