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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Hortikultura Indonesia (JHI) Jurnal Natur Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Hortikultura Pengembangan Inovasi Pertanian Jurnal Penelitian dan Pengembangan Pertanian AGRIVITA, Journal of Agricultural Science
Budi Marwoto
Indonesian Ornamental Crops Research Institute (IOCRI), Jl. Raya Ciherang, Segunung Pacet Cianjur 43253, West Java, Indonesia Phone (62-263) 512607
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Immunology & microbiology Veterinary

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Pengujian Keefektifan Gliokompos terhadap Pertumbuhan dan Perkembangan Tanaman Krisan Wasito, Antoro; Marwoto, Budi
Jurnal Hortikultura Vol 13, No 4 (2003): DESEMBER 2003
Publisher : Indonesian Center for Horticulture Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jhort.v13n4.2003.p229-235

Abstract

Penelitian bertujuan mengetahui pengaruh aplikasi gliokompos terhadap pertumbuhan dan perkembangan tanaman krisan yang dilaksanakan di Kebun Percobaan Balai Penelitian Tanaman Hias Segunung dari bulan September 1999 sampai dengan Januari 2000. Penelitian menggunakan rancangan acak kelompok pola faktorial dengan tiga ulangan. Delapan komposisi media tanam berupa perbandingan volume gliokompos, pupuk kandang, dan tanah sebagai faktor pertama dan tiga varietas, yaitu saraswati, retno dumilah, dan dewi sartika sebagai faktor kedua. Data diperoleh dari beberapa peubah pertumbuhan dan hasil bunga. Penggunaan gliokompos efektif dalam meningkatkan pertumbuhan tanaman, hasil bunga serta ketahanan tanaman terhadap serangan penyakit tular tanah. Pertumbuhan dan hasil bunga terbaik ditunjukkan oleh varietas retno dumilah, disusul dengan varietas saraswati dan dewi sartika.  Penggunaan pupuk kandang sebagai media tanam cenderung menurunkan ketahanan tanaman akan serangan beberapa penyakit tular tanah.  Sebaliknya penggunaan gliokompos mampu menekan serangan penyakit tular tanah serta meningkatkan hasil bunga. Kata kunci: Dendrathema grandiflora Tzvelev; Gliokompos; Kesehatan tanaman; Pertumbuhan tanaman; Hasil bunga ABSTRACT. The objective of this experiment was to evaluate effective- ness of gliocompost as soil sterillant on the cutting production and healthiness. Experiment was conducted at Indone- sian Ornamental Research Institute Segunung  from September 1999 to January 2000.  A factorial randomized block design with three replications was used in this experiment.  Eight medium compositions described by volume ratio of gliocompost, chicken manure, and soil, were notated as first factor. Three varieties, namely saraswati, retno dumilah and dewi sartika, were used as second factor. Data collected were plant growth and healthiness as well as flower pro- duction. Based on the available data, concluded that the best plant growth performance and flowers production were showed by retno dumilah, followed by saraswati and dewi sartika. The use of chicken manure as a planting medium tended reduce plant resistance to soil borne diseases. Inversely, the use of gliocompost as planting medium increased plant resistance and  flower production..
Pengujian Keefektivan Gliokompos terhadap Pertumbuhan dan Perkembangan Tanaman Krisan Wasito, Antoro; Marwoto, Budi
Jurnal Hortikultura Vol 13, No 3 (2003): SEPTEMBER 2003
Publisher : Indonesian Center for Horticulture Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jhort.v13n3.2003.p1-6

Abstract

Penelitian bertujuan mengetahui pengaruh aplikasi gliokompos terhadap pertumbuhan dan perkembangan tanaman krisan yang dilaksanakan di Kebun Percobaan Balai Penelitian Tanaman Hias Segunung dari bulan September 1999 sampai dengan Januari 2000. Penelitian menggunakan rancangan acak kelompok pola faktorial dengan tiga ulangan. Delapan komposisi media tanam berupa perbandingan volume gliokompos, pupuk kandang dan tanah sebagai faktor pertama dan tiga varietas, yaitu saraswati, retno dumilah dan varietas   dewi sartika sebagai faktor kedua. Data diperoleh dari beberapa peubah pertumbuhan dan hasil bunga. Berdasarkan hasil analisis dapat diketahui bahwa penggunaan gliokompos efektif dalam meningkatkan pertumbuhan tanaman, hasil bunga serta ketahanan tanaman terhadap serangan penyakit tular tanah.  Pertumbuhan dan hasil bunga  terbaik ditunjukkan oleh varietas Retno Dumilah, disusul dengan varietas saraswati dan dewi sartika.  penggunaan pupuk kandang sebagai media tanam cenderung menurunkan ketahanan tanaman akan serangan beberapa penyakit tular tanah.   Sebaliknya penggunaan gliokompos mampu menekan serangan penyakit tular tanah serta meningkatkan hasil bunga. Kata kunci: Dendrathema grandiflora Tzvelev; Gliokompos; Kesehatan tanaman; Pertumbuhan tanaman;Hasil bunga ABSTRACT. The objective of this experiment was to evaluate effectiveness of gliocompost as soil sterillant on the cutting production and healthiness. Experiment was conducted at Indonesian Or- namental Research Institute Segunung  from September 1999 to January 2000.  A factorial randomized block design with three replications was used in this experiment.  Eight medium compositions described by volume ratio of gliocompost, chicken manure and soil, were notated as first factor. Three varieties, namely saraswati, retno dumilah and dewi sartika, were used as second factor. Data collected were plant growth and healthiness as well as flower pro- duction. Based on the available data, concluded that the best plant growth performance and flowers production were showed by retno dumilah, followed by saraswati and dewi sartika. The use of chicken manure as a planting medium tended reduce plant resistance to soil borne diseases. Inversely, the use of gliocompost as planting medium increased plant resistance and  flower production.
Efficacy of Selected Plant Extracts to Control Leaf Miner (Lyriomyza spp.) in Chrysanthemum Rahardjo, Indijarto Budi; Marwoto, Budi; Budiarto, Kurniawan
AGRIVITA, Journal of Agricultural Science Vol 42, No 1 (2020)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v42i1.2219

Abstract

The use of eco-friendly methods in controlling pest and diseases has become an increasing concern in production system in many economically important crops, including ornamentals. The application of plant extract that has an insecticidal effect is considered as one promising alternative in reducing the negative effects of synthetic pesticides. The research was conducted to assess selected plant extracts in several concentrations against leaf miner (Lyriomyza spp.) in chrysanthemum. The experiment was carried out from January to December 2017 under plastic house conditions at the Indonesian Ornamental Crops Research Institute (IOCRI). The extracted organ of insecticidal plants regularly sprayed into chrysanthemum plants and compared with water as the controls. The results showed that the application of insecticidal plants extracts reduced attack intensity and incidence from water treatment (control). Certain treatment combinations, Chinese mahogany leaf extract at the concentration of 0.25%, C. pyrethrum petal 0.15 and 0.30%, and chinaberry leaf at 0.3, 0.35% even had 0.9-3.13% lesser attack incidences than commercial botanical insecticide Neem Plus. The respected treatments also supressed more than 62% leaf miner attacks and induced the increase of marketable flowers.
Improvement of Selected Induction Culture Media on Callus Induction in Anther Culture of Anthurium and a Histological Study on its Callus Formation Winarto, Budi; Mattjik, Nurhayati Ansori; Purwito, Agus; Marwoto, Budi
Jurnal Natur Indonesia Vol 12, No 2 (2010)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (607.475 KB) | DOI: 10.31258/jnat.12.2.93-101

Abstract

Improvement of selected induction culture media on callus induction in anther culture of anthurium and a histologicalstudy on its callus formation were studied at the tissue culture laboratory of the Indonesian Ornamental CropsResearch Institute from February to October 2008. The objectives of the study were to optimize selected media forcallus formation, reveal cell origin of callus derived from anther culture and shoot formation process. Selectedmedia improved in the study were 1) MMS-TBN containing 0,5 mg/l TDZ, 1,0 mg/l BAP and 0,01 mg/l NAA (Winartomedium, WM) and 2) MMS III supplemented with 1,5 mg/l TDZ, 0,75 mg/l BAP and 0,02 mg/l NAA (Winarto andRachmawati medium, WRM). Improvement treatments were carried out by omission and application of 2,4-D in 0.5mg/l and reduction of medium strength of full, half, quarter, one eighth, one sixteenth, and zero strength. Afactorial experiment was arranged using a randomized complete block design with four replications. Results ofthis study indicated that the highest callus induction was clearly established in WRM. The medium stimulatedpotential growth of anther (PGA) up to 81% with 49% of percentage of anther regeneration (PAR) and 2.7 number ofcallus formed per replication (NCF). Significant improvement in callus formation was also recorded by reduction ofmedium strength of WRM to one eighth compared to others. The reduction induced PGA up to 58% with 29% of PARand 1.8 NCF. From histological studies it was well recognized that regenerated callus on half anthers cultured wasoriginated from middle layer cells of anther wall. The morphogenic response of anther wall cells caused primarilyon no androgenesis effect in microspore cells.
Medium Term Conservation of Several Carnation Accessions Via in Vitro Culture Budiarto, Kurniawan; Marwoto, Budi
Jurnal Natur Indonesia Vol 13, No 2 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (49.629 KB) | DOI: 10.31258/jnat.13.2.174-177

Abstract

Sufficient genetic diversity is important in carnation breeding program. In vivo conservation of carnation germplasmis considered inefficient due to some technical and economical aspects. In vitro conservation was then, expectedto overcome the limitation of in vivo method. The research was conducted to find out the proper media for medium-term in vitro conservation of several carnation accessions in low temperature storage. A complete factorialexperiment with 25 replications was designed to accomplish the combination of two factors. The first factor wassix commercial carnation cultivars, namely Pink Maladi, Orange Triumph, Opera, Tundra, Yellow Liberty and PradoReffit. The second factor was the conservation media i.e. 1⁄2MS + DMSO 3% and 1⁄2MS + 3% DMSO + 3% sucrose andcontrol (MS 0+3% sucrose). The results showed that in vitro conservation of carnation in low temperature weresuccessfully conducted using 1⁄2MS+3% DMSO and 1⁄2MS+3% DMSO+3% sucrose without significant variation in allaccessions tested up to 10 and 12 months respectively. The increase of death plantlets, however, was detected onthe media of 1⁄2MS+3% DMSO after 6 months storage with significant decrease in viability hereafter. The existenceof sucrose in DMSO media induced root formation and plantlet resistance to low temperature storage.
Pengujian Kisaran Inang Nematoda Bentuk Ginjal (Rotylenchulus reniformis Linford dan Oliveira) Marwoto, Budi
Jurnal Hortikultura Vol 19, No 4 (2009): Desember 2009
Publisher : Indonesian Center for Horticulture Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jhort.v19n4.2009.p%p

Abstract

Rotylenchulus reniformis merupakan salah satu nematoda semiendoparasit penting yang menyerangberbagai jenis tanaman hortikultura di Indonesia. Nematoda ini dapat ditemukan di dataran rendah maupun datarantinggi di Indonesia. Salah satu cara yang efektif untuk mengendalikan R. reniformis ialah melalui penerapan rotasitanaman dan sanitasi lingkungan, termasuk memusnahkan tanaman inang alternatif. Untuk itu, diperlukan pengujianstatus inang berbagai jenis tanaman dan spesies gulma terhadap R. reniformis. Penelitian dilaksanakan pada bulan April2002 sampai Januari 2003 di Rumah Kaca dan Laboratorium Nematologi Balai Penelitian Tanaman Hias Segunung,Kecamatan Pacet, Kabupaten Cianjur (1.100 m dpl.). Penelitian menggunakan rancangan acak lengkap dengan 5ulangan. Sebanyak 84 jenis tanaman sayuran, tanaman hias, dan berbagai spesies gulma digunakan sebagai perlakuan.Setiap tanaman diinokulasi dengan 1.000 ekor nematoda yang merupakan campuran larva, nematoda jantan, dan betinapradewasa. Status inang ditentukan dengan kriteria faktor reproduksi R. reniformis lebih dari 1 = tanaman inang R.reniformis dan faktor reproduksi kurang dari 1 = bukan tanaman inang. Faktor reproduksi merupakan perbandinganantara populasi akhir dan populasi awal. Hasil penelitian menunjukkan bahwa dari 41 jenis tanaman sayuran yangdiuji, 24 di antaranya merupakan tanaman inang R. reniformis. Cabai, wortel, dan bawang-bawangan bukan inangR. reniformis. Tujuh spesies gulma berdaun lebar dapat digolongkan sebagai inang R. reniformis. Semua gulmamonokotil yang diuji bukan inang R. reniformis. Populasi R. reniformis tidak dapat berkembang pada hibrida Tagetespatula dan T. erecta, Crotalaria usaramoensis, dan Ricinus communisABSTRACT. Marwoto, B. 2009. Study of Host Range of Reniform Nematode (Rotylenchulus reniformis Linfordand Oliveira ). Rotylenchulus reniformis is one of the most important semiendoparasitic nematode attacking differentspecies of horticultural crops and weeds in Indonesia. The nematode can be found in the lowland and highland areasin Indonesia. One of the most reliable control measures of the nematode in the field is by applying crop rotation anderadicating alternative hosts. Before applying those control measures, a research on the host status of different cropsand weed species to the nematode is necessarily to be done. This study was conducted on April 2002 to January2003 at the Greenhouse and Nematology Laboratory of The Research Institute for Ornamental Crops, Cianjur, WestJava (1,100 asl.). A completely randomized design with 5 replications was used in this study. A total of 84 speciesand varieties of vegetables, ornamental crops, and weeds were used as treatments. Every variety of the crops and theweeds were inoculated with 1,000 nematode population, comprised of larvae, male, and pre-adult female. Host statuswas determined by the following criteria reproductive factor: >1=host plant and reproductive factor; and <1=non-hostplant; where reproductive factor was ratio between initial population and final population. The results showed that ofthe 41 species and varieties of vegetable crops tested, 24 species were determined as non-host of R. reniformis. Amongthem were chili pepper, carrot, shallots, and garlic. Seven weed species were categorized as host of R. reniformis.Monocot weeds were mostly proven as non-host of R. reniformis. Population of R. reniformis could not grow onTagetes patula and T. erecta hibryds, Crotalaria usaramoensis, and Ricinus communis
Evaluasi Keragaan Morfologi Sembilan Klon Hasil Persilangan Anyelir (Dianthus caryophyllus L.) Kartikaningrum, Suskandari; Marwoto, Budi; Dewanti, Minangsari
Jurnal Hortikultura Indonesia (JHI) Vol. 12 No. 3 (2021): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jhi.12.3.191-197

Abstract

Anyelir (Dianthus caryophyllus L.) merupakan salah satu komoditas bunga potong komersial yang sangat penting di dunia. Tanaman anyelir mempunyai susunan genetik yang heterozigot, tetapi tanaman F1 terseleksi dari hasil persilangan langsung dapat dijadikan tanaman induk sebagai sumber perbanyakan vegetatif. Setiap klon F1 berbeda secara genetik. Tujuan percobaan ialah mendapatkan klon-klon anyelir bunga potong yang memiliki kombinasi karakter morfologi tanaman dan bunga yang unggul. Percobaan dilaksanakan di rumah lindung Instalasi Penelitian dan Pengkajian Teknologi Pertanian (IP2TP) Cipanas, Cianjur, Jawa Barat, dengan ketinggian 1,100 m dpl, dari bulan Januari sampai dengan Desember 2019. Penelitian ditata dalam rancangan acak kelompok lengkap dengan sembilan perlakuan, yaitu : D 1.1, D 3.13, D 5.1, D 5.4, D 5.5, D 8.5, D 8.8, D 13.13 dan D 13.14 dan tiga ulangan. Hasil percobaan menunjukkan bahwa klon D 13.14 terpilih sebagai klon harapan anyelir bunga potong, karena memiliki kombinasi karakter morfologi tanaman dan bunga yang unggul, yaitu tanaman kokoh, diameter bunga besar (7.52 cm), jumlah petal terbanyak (87.67 helai), kesegaran bunga terlama (13.83 hari) dan warna bunga merah menyala.
Embriogenesis Somatik dari Eksplan Daun Anggrek Phalaenopsis sp L. (Somatic Embryogenesis from Leaf Eksplant of Phalaenopsis Orchids) Rianawati, Sri; Purwito, Agus; Kurniati, Ridho; Marwoto, Budi; Suryanah, Suryanah
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 37 No. 3 (2009): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (606.295 KB) | DOI: 10.24831/jai.v37i3.1241

Abstract

Somatic embryogenesis has been recoqnized as one of the process on plant micropropagation  techniques. This process occured through regeneration by direct embryo formation and through an intermediary callus phase. This research was conducted through an intermediary callus phase. The experiment was initiated with callus induction from leaf explant on five modifications of MS medium i.e :1/2MS without plant hormone  (MI-0); ½ MS containing 1mg/L BA + 0.5 mg/L 2.4-D + 1mg/L NAA  (MI-1);1/3 MS containing 2 mg/L  2.4-D (MI-2); ½ MS supplemented with 0.5 mg/L 2.4-D + 0.5 mg/L BAP +0.2 mg/L thidiazuron (MI-3); ½ MS supplemented 2 mg/L thidiazuron and 1 mg/L BAP (MI-4). After the tissues were swollen, the  explants  were  placed on callus proliferation medium  ½ MS supplemented with 0.2 mg/L thidiazuron and 0.5 mg/L 2.4-D (MP). After two months, calli were  regenerated in regeneration medium ½ MS supplemented with 0.4  mg/L BAP and 0.2 mg/L  2.4-D (MR). The results of this research  showed that  MI-1 and MI-3 were the best swelling explant mediums   before the callus  produced in both MP and MR medium. Callus produced was increased in every subculture. However, the level of callii production decreased on the following subculture. Plantlets were regenerated from somatic embryos derived from  callii on MR medium. The results of this study may contribute to our advancement of scientific knowledge achievements tissue culture techniques to support inconventional plant improvement.   Key words:  embryo somatic induction, in vitro, embryogenic callii  
Induksi Mutasi pada Stek Pucuk Anyelir (Dianthus caryophyllus Linn.) melalui Iradiasi Sinar Gamma Aisyah, Syarifah Iis; Aswidinnoor, Hajrial; Saefuddin, Asep; Marwoto, Budi; Sastrosumarjo, Sarsidi
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 37 No. 1 (2009): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (620.001 KB) | DOI: 10.24831/jai.v37i1.1396

Abstract

It has been a common knowledge that LD50 is commonly used in estimating optimal doses of gamma irradiation in a breeding program. This research was aimed at observing radiosensitivity of five carnation's genotypes to gamma irradiation, to find the LD50 of carnation's cuttings, and to obtain solid mutants from five numbers of Carnation.  For cuttings, carnation genotype number 10.8 was the most insensitive to gamma rays, whereas number 24.15 was the most sensitive one.  LD50 of carnation's cuttings were obtained around 49 -72 gray. There were 19 mutants produced from this treatment. The desired mutans were mostly produced from the treated 24.1 genotype whereas the character mutans were mostly observed in MV2 generation. Irradiation treatment on genotype 24.1 produced most stabile mutans while the less was in genotype 24.14.  The produced mutants were qualitatively different in colour and petal shape, and stabile till third generation.   Key words: LD50, gamma irradiation, induced mutation, carnation.
Induksi Ginogenesis melalui Kultur Multi Ovule Slice dan Kultur Ovary Slice Dianthus chinensis Kartikaningrum, Suskandari; Purwito, Agus; Wattimena, Gustaaf Adolf; Marwoto, Budi; Sukma, Dewi
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 41 No. 1 (2013): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (381.553 KB) | DOI: 10.24831/jai.v41i1.7078

Abstract

Callus induction was studied in five genotypes of Dianthus chinensis using 2.4 D and NAA. Calluses can be obtainedfrom unfertilized ovule culture and ovary culture. The aim of the research was to study gynogenic potential and responseof Dianthus chinensis through ovule slice and ovary slice culture for obtaining haploid plants. Five genotypes of Dianthuschinensis and five media were used in ovule slice culture and two genotypes and three medium were used in ovary culture.Flower buds in the 7th stage were incubated for the purpose of dark pre-treatment at 4 oC for one day. Ovules and ovaries wereisolated and cultured in induction medium. Cultures were incubated for the purpose of dark pre-treatment at 4 oC for seven days, followed by 25 oC light incubation. The result showed that 2.4D was better than NAA in inducing callus. Percentage of regenerated calluses were produced in V11, V13 and V15 genotypes in M7 medium (MS + 2 mg L-1 2.4D + 1 mg L-1 BAP + 30 g L-1 sucrose and M10 medium (MS + 1 mg L-1 2.4D + 1 mg L-1 BAP + 20 g L-1 sucrose). All calluses originated from ovule and ovary cultures flowered prematurely. Double haploid (V11-34) were obtained from ovule slice culture based on PER (peroksidase) and EST (esterase) isoenzym marker.Keywords: ovule slice culture, ovary slice culture, callus, Dianthus sp., haploid
Co-Authors - Hanudin - Hermawan A Purwito A Sumantri Agus Muharam Agus Purwito Antoro Wasito ASEP SAEFUDDIN Aziz Azirin Asandhi Bagus Kukuh Budiarto Budi Winarto D Sukma Dewi Sukma Dyah Widiastoety Evi Silfia Yusuf Evi Silvia Evi Silvia Yusuf G A Watimena Gustaaf Adolf Wattimena HAJRIAL ASWIDINNOOR Hanudin - Hanudin . Hanudin Hanudin Hersanti - I. Djatnika, I. Ika Djatnika Indijarto Budi Rahardjo, Indijarto Budi Kurniawan Budiarto Lia Sanjaya Minangsari Dewanti, Minangsari N A Mattjik Nurhayati Ansori Mattjik Ridho Kurniati Rudy Soehendi Sarsidi Sastrosumarjo Silfia Yusuf Sri Rianawati Suryanah Suryanah Suskandari Kartikaningrum Syarifah Iis Aisyah Wakiah Nuryani Waqiah Nuryani Wiwin Setiawati