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Journal : agriTECH

Efek Pemberian Buah Jambu Biji Merah terhadap Produksi Scfa dan Kolesterol dalam Caecum Tikus Hiperkolesterolemia Sugeng Maryanto; Siti Fatimah; Sugiri Sugiri; Yustinus Marsono
agriTECH Vol 33, No 3 (2013)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (164.963 KB) | DOI: 10.22146/agritech.9556

Abstract

Red guava fruit has high soluble fiber (pectin) and vitamin C content. Soluble fiber has a hypocholesterolemic effect. The aim of the study was to analyze the effect of red guava fuits supplementation on the SCFA (short chain fatty acid) production and cholesterol content in caecum, to explain the mechanism of red guava on the blood cholesterol reduction. The study was a randomized pre test-post test control group design on the hypercholesterolemic Sprague Dawley rats given a high-cholesterol feed. Rats were divided into 4 groups, receiving normal feed, hypercholesterol feed only, hypercholesterol feed + 0,72 g of red guava flour and hypercholesterol feed + 0,26 g pectin equal to that in red guava flour. Caecum digestion products beeing examined were SCFA (short chain fatty acids) analysed by gas chromatography and cholesterol by Liebermann-Burchard method using a spectrophotometer. Data were analysed with 95% of level confidence one way Anova. The high SCFA and cholesterol concentrations in the rats caecum were found on the red guava supplementation groups and was significantly different from pectin suplemented group. The concentrations of acetic acid, propionic acid, butyric acid, and caecum cholesterol were 18.9 mg/g, 7.8 mg/g, 2.4 mg/g, and 0.54 mg/g, compared to with pectin were 19.3 %M, 21.4 %M, 19.7 %M and 0.46 mg/g, respectively. The propionic acid caecum production and cholesterol excretion shown that to lower serum cholesterol. Red guava supplementation produced propionic acid and high cholesterol excretion as found in the caecum.ABSTRAKBuah jambu biji merah memiliki kandungan serat khususnya serat larut air (pektin) yang tinggi. Serat larut air di dalam tubuh bersifat hipokolesterolemik. Penelitian ini bertujuan untuk mengetahui efek pemberian buah jambu biji merah terhadap produksi SCFA (short chain fatty acid) dan kolesterol digesta caecum serta mengkaji mekanisme penurunan kolesterol akibat pemberian buah jambu biji merah pada tikus hiperkolesterolemia. Penelitian ini merupakan eksperimental murni dengan rancangan randomized pre test-post test control group design terhadap tikus Sprague Dawley yang dibuat hiperkolesterolemia. Tikus dibagi dalam 4 kelompok, kelompok 1 diberi pakan standar, kelompok 2,3 dan 4 semua diberi pakan tinggi kolesterol. Kelompok 3 dan 4 masing-masing ditambah tepung buah jambu biji merah 0,72 g dan pektin 0,26 g setara dengan yang terkandung tepung buah jambu biji. Pemeriksaan digesta caecum meliputi kadar SCFA (short chain fatty acid) menggunakan kromatografi gas, serta kadar kolesterol dengan metode Liebermann-Burchard menggunakan spektrofotometer. Analisis data menggunakan Anova dengan tingkat kepercayaan 95%. Pemberian buah jambu biji merah pada tikus hiperkolesterolemia terbukti secara bermakna menghasilkan SCFA asam asetat 42,9 %M; asam propionat 36,5 %M dan asam butirat 43,8 %M serta kolesterol caecum 0,54 mg/g. Hasil ini lebih tinggi dibandingankan dengan pektin yaitu asetat 19,3 %M; asam propionat 21,4 %M dan asam butirat 19,7%M serta kolesterol caecum 0,46 mg/g, dan berbeda secara bermakna antara kelompok jambu biji dan pektin. Produksi asam propionat dan ekskresi kolesterol caecum dapat mengakibatkan penurunan kolesterol serum. Pemberian buah jambu biji merah terbukti menghasilkan asam propionat dan meningkatkan ekskresi kolesterol dalam caecum.
Red Guava Juice (Psidium guajava L.) Reduce Oxidative Stress of Toll Gate Collector Riva Mustika Anugrah; Sugeng Maryanto; Kusmiyati Tjahjono; Martha Irene Kartasurya
agriTECH Vol 39, No 4 (2019)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (740.347 KB) | DOI: 10.22146/agritech.23030

Abstract

Exposure to air pollution can increase the occurrence of oxidative stress. Research showed that guava can reduce oxidative stress in Sprague Dawley rats. This experiment investigated the effect of red guava juice on oxidative stress in toll collectors. The subjects were 20 toll collectors in the treatment group and 20 in the control group. The treatment group received 250 mL red guava juice for 21 days while the controls did not receive anything. Malondialdehyde (MDA) was measured with thiobarbituric acid (TBA) reaction, food consumption was measured by 3×24 hours’ recall. The data were analyzed by paired t-test, Wilcoxon test, independent t-test, Mann Whitney test, and general linear model. The results showed that the effect of red guava juice on the MDA level was significant in the treatment group (p<0.05). A multivariate analysis showed that the effect of red guava juice to MDA level was significant after controlled by age, carbohydrate intake and initial conditions (p<0.05). Red guava juices of 250 mL for 21 days could reduce malondialdehyde (MDA) levels of toll collectors.
Co-Authors Afiatna, Puji Afim Rofkhul Roim Agi Yulia Ria Dini Agi Yulia Riadini Agnes Setiana, Dwi Ajeng Ayu Nur Fitriyani Alpia Pebriana Amalia, Friska Andini, Virnalia Anggun Novita Sari Anisa Puspitasari Astuti, Iga Dila Widuri Baiq Mega Narasuari Baiq Wiwik Subandary Cicik Lestari Dewi Puspita Dewi Puspita Dewi Puspita Dewi Ratnasari Dewi, Hany Kharisma Dian Oktianti Dyah Kartika Wening Elisabeth Usfal Erwin Indayanti Esti Rahayu Farhatus Saidah Faridah Aini Galeh Septiar Pontang Hany Kharisma Dewi Hany Kharisma Dewi Hapsari Windayanti Hasandi, Litta Arsieta Hasna Nur Afina Hifayah Iin Purnamasari Iin Purnamasari Indri Mulyasari Indri Mulyasari Kadarwati, Frisca Erwin Krido Utami, H. Haryanti Kumaladewi, Desy Kusmiyati Tjahjono Liestyaningsih, Chori’ah Lusiana, Ita Mardianti, Wiwit Maria Bella Pereira Maria Magdalena Meilina Rahmawati Martha Irene Kartasurya Martha Nilawati Meilita Dwi Paundrianagari Miftana Fitri Aditami Muhamad Aziz Anwar Novia Eka Rahmawati Nur Cholifah Nur Cholifah Nur Hasanah Nur Wulan Nurdianti Nurhijah Ermadani Nurina, Marliana Eka Pratiwi, Aninda Putri Pratiwi, Galuh Endah Pratiwi, Ni Luh Gede Trisna Puji Afiatna Puji Afiatna Purbowati Purbowati Purbowati Purnasari, Mitha Putri, Baiq Riski Amalina Putri, Risma Aliviani Quiteria Libania Marques Rifatul Ridlo Riva Mustika Anugrah Sari, Nikita Arum Shintia Dewi May Vebrianingsih Shintya Fika Harvi Silvia Rokana Alvida Silviana S, Andhike Sisca Ulivia Siska Dea Novitasari Siti Fatimah Siti Nurfaizah Srimaharani Suciani, Indah Sugiri Sugiri Suwarno Widodo Suwarno Widodo Tina Mawardika Umi Novianingtyas Umi Setyoningrum Untari Uswatun Khasanah Vanes Ufi Safarah Virginia, Any Windy Harly Yustinus Marsono