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All Journal Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) The Indonesian Biomedical Journal Jurnal Dinamika Pengabdian (JDP) Nusantara Medical Science Journal Indonesian Journal of Obstetrics & Gynecology Science Indonesian Journal of Obstetrics and Gynecology (Majalah Obstetri dan Ginekologi Indonesia) GHIDZA: Jurnal Gizi dan Kesehatan Majalah Patologi Indonesia Golden Age: Jurnal Ilmiah Tumbuh Kembang Anak Usia Dini Healthy Tadulako Journal (Jurnal Kesehatan Tadulako)
Rina Masadah
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Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Dentistry Education Health Professions Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Other

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Journal : The Indonesian Biomedical Journal

 1 
NPC-Exosome Carry Wild and Mutant-type p53 among Nasopharyngeal Cancer Patients Hamsu Kadriyan; Eka Sunarwidhi Prasedya; Nova Audrey Luetta Pieter; Masyita Gaffar; Amsyar Akil; Agusalim Bukhari; Budu Budu; Andi Alfian Zainuddin; Rina Masadah; Achmad Chusnu Romdhoni; Abdul Qadar Punagi
The Indonesian Biomedical Journal Vol 13, No 4 (2021)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v13i4.1718

Abstract

BACKGROUND: Nasopharyngeal cancer (NPC) is known to release a specific exosome. NPC-derived exosome (NPC-Exo) could carry p53. However, information regarding the type of p53 carrier on NPC-Exo remains limited. This study aims to introduce our important findings regarding the type of p53 NPC-Exo cargo.METHODS: Serum from patients with NPC were prepared for exosome isolation with Seramir Exoquick by following the manual instructions. RT-PCR was conducted to determine the expression levels of latent membrane protein 1 (LMP-1) and p53 in the exosome isolate. Partial sequencing of p53 amplicon was conducted to determine mutation type of p53.RESULTS: There were 8 patients enrolled in this study. According to RT-PCR results, the expression levels of LMP-1 and p53 varied in the NPC-Exo isolate. Based on sequencing analysis, 1 case of p53 mutation was noticeable.CONCLUSION: According to current results, the NPC-derived exosome potentially carries not only wild type but also mutant type p53. Further research is needed to explore deeper the effect of the mutant type p53 as an exosome carrier in the clinical application.KEYWORDS: Nasopharyngeal cancer, exosome, p53, mutation
Risk Assessment in Hereditary Colorectal Cancer Family by Using APC and MSH2 mRNA Gene Expression and Bayesian Analysis Tjahjadi Robert Tedjasaputra; Mochammad Hatta; Muhammad Nasrum Massi; Rosdiana Natzir; Ilhamjaya Patellongi; Marcellus Simadibrata; Ibrahim Labeda; Rina Masadah; Muhammad Luthfi Parewangi; Prihantono Prihantono; Andi Asadul Islam; Agussalim Bukhari; Budu Budu; Rinda Nariswati
The Indonesian Biomedical Journal Vol 12, No 4 (2020)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v12i4.1329

Abstract

BACKGROUND: Some of colorectal cancers (CRCs) are familial, however, heterozygote relatives have approximately 80% lifetime risk of cancer. Risk assessment of CRC’s family could be calculated by direct measurement of mRNA gene expression and Bayesian theorem which is modifying initial background of pedigree risk with additional conditional information. This application has not been reported.METHODS: The cross-sectional translational sequential studies were performed: (1) adenomatous polyposis coli (APC) and MutS homolog (MSH)2 mRNA quantitative RT PCR gene expressions in tissue and whole blood CRC patients; (2) gene expression was determined in matched controls; and (3) pedigree and Bayesian analysis was calculated in the patient’s family of Proband.RESULTS: Fourty CRC and 31 control subjects were enrolled. The mean blood APC level control’s group was 13,261±670 fold-change (fc) and blood MSH2 level was 12,219±756 fc. The cut-off points for hereditary APC was 12,195 fc and MSH2 was 11,059 fc. The mean APC blood level in CRC subject was 11,578±2,638 fc and MSH2 blood level was 11,411±2,912 fc. There were significant differences APC and MSH level between tissue and blood level in CRC. Eight of 40 CRC subjects had a history of familial CRC. Four patients and 10 Probands were available for recurrence risk evaluation of pedigree analysis, RNA PCR quantitative and Bayesian calculation.CONCLUSION: There was determined a cut-off point of hereditary mRNA quantitative expression. The APC and MSH2 levels in CRC subjects were significantly lower than controls. Bayesian analysis allowed for the calculation of relative risk in CRC family members and considered in clinical practice.KEYWORDS: hereditary CRC, APC gene, MSH gene, Bayesian analysis
Intrauterine Transmission of Hepatitis B Cannot Be Ruled Out by A Single Negative Hepatitis B e Antigen (HBeAg) Result among Hepatitis B Surface Antigen (HBsAg) - Positive Pregnant Women Chalid, Maisuri Tadjuddin; Judistiani, Tina Dewi; Syahril, Rizalinda; Masadah, Rina; Febriani, Dwi Bahagia; Wahyuni, Ridha; Turyadi, Turyadi; Massi, Muh Nasrum
The Indonesian Biomedical Journal Vol 16, No 1 (2024)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v16i1.2726

Abstract

BACKGROUND: The risk factors for intrauterine transmission of hepatitis B virus (HBV) in hepatitis B surface antigen (HBsAg)-positive pregnant women are poorly understood. Numerous factors are considered to be involved, including placental barrier, obstetric environment, high viral load, and positivity of hepatitis B e antigen (HBeAg). This study was conducted to investigate the role of placenta barrier, clinical, and viral factors in intrauterine transmission of HBV.METHODS: A cross-sectional study was conducted involving 1,353 pregnant women who underwent HBsAg screening. Eighty-four (6.2%) women were detected as HBsAg positive and were examined for HBsAg level, anti-HBs, anti-HBc, HBeAg/hepatitis B e antibody (anti-HBe) status, and HBV DNA presence in cord blood. Quantitative HBV DNA was analyzed using real-time polymerase chain reaction (PCR).RESULTS: Eighty-four of 1,353 subjects were HBsAg-positive. HBV DNA was positive in 28/84 (33.7%) maternal sera, 19/79 (24.05%) placental specimens, and 9/83 (10.84%) in cord blood. There were significant associations between HBV DNA in maternal serum (p=0.000) and placental tissue (p=0.000) with HBV DNA in the cord blood. No clinical factors were associated with HBV DNA transmission in cord blood. Sixty percent of viral load >5.3 log10 copies/mL were found in the cord blood, of which 43.8% HBeAg positive and 3.1% HBeAg negative.CONCLUSION: Reduced transmission via compartments established the placenta’s barrier function in mother-to-child transmission. A high maternal viral load and positive HBeAg were risk factors for intrauterine transmission, while negative HBeAg still has the possibility of transmission.KEYWORDS: mother-to-child transmission, hepatitis B virus, intrauterine
Co-Authors Abdul Muis Abdul Qadar Punagi Achmad, Djumadi Agusalim Bukhari Al atsari, Muhammad Azka Andi Alfian Zainuddin Andi Alfian Zainuddin Andi Asadul Islam Andi Weri Sompa Andira, Fiki Wahyu Budu - Budu Budu Bukhari, Agussalim Cahyono Kaelan, Cahyono Cangara, Husni Cangara, Muhammad H Chalid, Maisuri Tadjuddin Djumadi Achmad Achmad Efendi Lukas Eka Sunarwidhi Prasedya Febriani, Dwi Bahagia Gaffar, Masyita Gosal, Steffy Rebecca Ham, Jonathan Hamsu Kadriyan Hanifah, Rif’at Huswatun Hasanah Ibrahim Labeda Ilhamjaya Patellongi Ismail J Nelwan, Berti Kalatiku, Jane Magdalena Lenny Lisal Luthfi, Muhammad Madaeng, Andi Sitti Nur Fatimah Maharani, Risma Maharani, Risma Mahmud Ghaznawie Maisuri T Chalid Maisuri T. Chalid Makarim, Ahmad Rifaat Dzaki Manule, Yolanda Marcellus Simadibrata Mardiati Maricar, Nadra Maulana, Aries Mochammad Hatta Muh. Nasrum Massi Muhammad Amsyar Akil Muhiddin, Syurawasti Naeny Fajriah Nasser, Andi S.H.D. Nasser, Andi Sitti Halija Diawanti Nelwan, Berti J. Nelwan, Berti Julian Nour Hafifah, Andi Izza Nova Audrey Luetta Pieter Prihantono Prihantono Raden Tina Dewi Judistiani Raharja, Siska Putri Wulandari Ridha Wahyuni, Ridha Rinda Nariswati Risma Maharani Risma Maharani Romdhoni, Achmad Chusnu Rosdiana Natzir Sianipar, Kelvin Leonardo St. Nur Asni Sumardin, Khairul Miftah Syahrial, Andi Muhammad Abdu Fadil Syahril, Rizalinda Syahrul Rauf Syahrul Rauf Syahrul Rauf Tawali, Suryani Tjahjadi Robert Tedjasaputra Truly D Dasril Turyadi Turyadi, Turyadi Umar, Husaini Upik A Miskad UPIK ANDERIANI MISKAD, UPIK ANDERIANI Winny N Leiwakabessy Wulaa, Nurwapina Langga