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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences JURNAL TEKNOLOGI LINGKUNGAN BIOTROPIA - The Southeast Asian Journal of Tropical Biology AL KAUNIYAH Proceeding International Conference on Global Resource Conservation Elkawnie Journal of Science and Applicative Technology Jurnal Penelitian Pendidikan IPA (JPPIPA) BAREKENG: Jurnal Ilmu Matematika dan Terapan Agro Bali: Agricultural Journal Biologica Samudra Bioscientist : Jurnal Ilmiah Biologi Jurnal Pengabdian dan Edukasi Sekolah (Jubaedah) Journal of Social and Policy Issues Bima Journal : Business, Management and Accounting Journal Seminar Nasional Riset dan Teknologi (SEMNAS RISTEK) Jurnal Sosiologi USK (Media Pemikiran & Aplikasi)
Oktaviani, Indah
Institut Teknologi Sumatera
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DNA Barcoding for Selected Mangrove-Based Estuary Fishes from Way Kambas National Park, Lampung Province, Indonesia Yanti Ariyanti; Ika Agus Rini; Indah Oktaviani; Sovia Santi Leksikowati
Journal of Tropical Life Science Vol. 11 No. 2 (2021)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.11.02.04

Abstract

Over the past decade, DNA barcoding has provided new insight into fish ecology and biosystematics and led to new species' discovery. DNA barcoding is a method for the recognition and identification of species using short, standardised DNA fragments. The correct taxonomic identification of species is critical for the assessment and monitoring of biodiversity. This study applied DNA barcoding techniques to identify selected fish species from a mangrove-based estuary in Way Kambas National Park, Lampung Province, Indonesia. The gene encoding cytochrome c oxidase subunit I (COI) was amplified and bi-directionally sequenced from 22 specimens. The resulting 680 base pairs (bp) sequence was used to identify species, obtain phylogenetic information, and analyse genetic distances. A neighbour-joining tree was constructed based on the mitochondrial COI gene using the Kimura two-parameter model. This study also exhibits conservation status for those identified species. Our findings will facilitate future studies of fish species diversity in mangrove estuary-based ecosystems and provide preliminary data in policymaking in conservation areas such as National Park.
Different Mangrove Rehabilitation Statuses Effects to Benthic Bacterial Structure Community in the Northern Area of Java Island: Mangrove Rehabilitation to Benthic Bacterial in the Northern Area of Java Island Vidayanti, Viky; Retnaningdyah, Catur; Mustafa, Irfan; Oktaviani, Indah
Journal of Tropical Life Science Vol. 14 No. 2 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.02.15

Abstract

The study proposed identifying the changes in bacterial community and diversity and exploring the potential correlations among sediments parameters and benthic bacterial communities under different mangrove ecosystems rehabilitation statuses. There were three sites: 1. Banyuurip Mangrove Center (BMC) as a restoration site; 2. Pulau Lusi (PL) as a degraded site; and 3. Kampung Blekok (KB) as natural site. The physicochemical parameters and benthic bacteria from the mangrove sediment of the three locations were observed. The data were analyzed statistically to determine the sites' variation and the correlation between parameters. The results found that the physicochemical parameters among restoration sites varied. Soil organic matter (SOM) in natural and restoration sites was significantly higher than in degraded sites. Cation exchange capacity (CEC), calcium and potassium ions, and soil conductivity of the restoration site were considerably higher than the other sites. According to diversity indices and taxa richness, benthic bacteria in the restoration were the most diverse. Proteobacteria dominated in natural and restoration sites; meanwhile, Firmicutes dominated in degraded site. Sulfurovum aggregans was found abundant in natural and restoration sites; meanwhile, Mesobacillus subterraneus was the dominant species in the degraded site. Furthermore, the bacterial taxa richness was positively correlated with SOM and the bacterial diversity was correlated with CEC and conductivity.
Different Mangrove Rehabilitation Statuses Effects to Benthic Bacterial Structure Community in the Northern Area of Java Island: Mangrove Rehabilitation to Benthic Bacterial in the Northern Area of Java Island Vidayanti, Viky; Retnaningdyah, Catur; Mustafa, Irfan; Oktaviani, Indah
Journal of Tropical Life Science Vol. 14 No. 2 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.02.15

Abstract

The study proposed identifying the changes in bacterial community and diversity and exploring the potential correlations among sediments parameters and benthic bacterial communities under different mangrove ecosystems rehabilitation statuses. There were three sites: 1. Banyuurip Mangrove Center (BMC) as a restoration site; 2. Pulau Lusi (PL) as a degraded site; and 3. Kampung Blekok (KB) as natural site. The physicochemical parameters and benthic bacteria from the mangrove sediment of the three locations were observed. The data were analyzed statistically to determine the sites' variation and the correlation between parameters. The results found that the physicochemical parameters among restoration sites varied. Soil organic matter (SOM) in natural and restoration sites was significantly higher than in degraded sites. Cation exchange capacity (CEC), calcium and potassium ions, and soil conductivity of the restoration site were considerably higher than the other sites. According to diversity indices and taxa richness, benthic bacteria in the restoration were the most diverse. Proteobacteria dominated in natural and restoration sites; meanwhile, Firmicutes dominated in degraded site. Sulfurovum aggregans was found abundant in natural and restoration sites; meanwhile, Mesobacillus subterraneus was the dominant species in the degraded site. Furthermore, the bacterial taxa richness was positively correlated with SOM and the bacterial diversity was correlated with CEC and conductivity.
Co-Authors Adriyanita Adin Ahmad Dodi Andriana Alie, Azizah Asril, Muhammad Ayu Octavia Tanjung Putri Catur Retnaningdyah D Beta Ramadan Elanda, Yelly Endri Bagus Prastiyo Evy Sulistianingsih Fina Khaerunissa Frima Halimatussa'diah, Halimatussa'diah Heriyati, Heriyati Ida Kinasih Idwal B, Idwal B Ika Agus Rini Ika Agus RINI Ika Agus Rini, Ika Agus Irfan Mustafa Leksikowati, Sovia Santi Mesita Dwi Pebina Mia Maria Ulfah Muhammad Asril Mursalin Mushaliyah Mushaliyah Musyarofah Zuhri Neva Satyahadewi Prastiyo, Endri Bagus Rahmat Azhari Kemal Ramadhani Eka Putra Revi Agustin Risha Amilia Pratiwi Rosmiati, Mia Sumarni, Yenti Tirtawaty Abdjul usman, nurbaiti Vidayanti, Viky Wibowo , Dimas Yanti Ariyanti Yanti Ariyanti, Yanti Yeni Rahayu Zamzami A Karim