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All Journal Jurnal Fitopatologi Indonesia Agrikultura Biotika: Jurnal Ilmiah Biologi Bionatura Microbiology Indonesia Cropsaver : Journal of Plant Protection Jurnal Perlindungan Tanaman Indonesia Jurnal Agrotek Tropika Soil REns Agrikultura Masyarakat Tani: Jurnal Pengabdian kepada masyarakat
Rika Meliansyah
Departemen Hama Dan Penyakit Tumbuhan, Fakultas Pertanian Universitas Padjadjaran Jalan Raya Bandung-Sumedang KM 21. Jatinangor, Indonesia 45363
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology

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Aplikasi Pupuk Organik terhadap Diversitas Arthropoda Akuatik serta Performa Tanaman Padi Hitam pada Ekosistem Sawah Vira Kusuma Dewi; Tiara Febricyanti Putri Senjaya; Fitri Widiantini; Agus Susanto; Sri Hartati; Lindung Tri Puspasari; Rika Meliansyah
Soilrens Vol 20, No 1 (2022)
Publisher : Dept Ilmu Tanah & Sumberdaya Lahan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/soilrens.v20i1.41354

Abstract

The aquatic ecosystems in rice fields has macroorganisms such as aquatic arthropods which have an important function in the food web. The siam weed and neem cake can be utilized as organic fertilizer to support the productivity and biodiversity of the paddy field, This research aims to understand application of organic fertilizer on diversity of aquatic arthropods and black rice performance in the field ecosystem. The experiment was carried out in paddy field at Jatinangor from January to June 2020. The method used was an experimental method with a Randomized Complete Block Design consisted of five treatments (neem cake, siam weed compost, cow manure, synthetic fertilizer and control) with six replications. The result showed that the abundances of aquatic arthropods have tendency to be higher in neem cake at 1104 individuals and siam weed compost treatments at 905 individuals compared to the cow manure, NPK and control. Furthermore, the diversity index of the aquatic arthropods in all treatments were categorized as moderate with details as follow neem cake (H’=1,726), cow manure (H’=1,450); synthetic fertilizer (H’=1,458); siam weed compost (H’=1,450) and control (H’=1,502). In addition, the evenness index (E) of aquatic arthropods was higher in neem cake treatment (E=0,623) compared to other treatments. Whereas, the dominance index (C) and richness index (R) in all treatments showed no dominant species and it classified as low category. The results also showed that the siam weed compost were not significantly different with all treaments except NPK (synthetic fertilizer) in plant hight, tiller number and SPAD value.
Kepadatan Populasi dan Pola Distribusi Keong Mas (Pomaceae canaliculta L.) pada Ekosistem Sawah di Kecamatan Jatinangor Vira Kusuma Dewi; Rizky Ramdhani; Tarkus Suganda; Lindung Tri Puspasari; Rika Meliansyah
Soilrens Vol 20, No 2 (2022)
Publisher : Dept Ilmu Tanah & Sumberdaya Lahan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/soilrens.v20i2.45272

Abstract

Golden snail (Pomacea canaliculata L.) one of a keypests in rice cultivation in Indonesia. There are three species of golden snails in Indonesia, these are P.canaliculata L., P. insularum D., and P. paludosa S. The purpose of this research was to determine the population density and distribution pattern of golden snails (P. canaliculata) at Jatinangor rice fields. The research was conducted from June 2020 to August 2020 with purposive sampling method on 24 rice fields in 12 villages, specifically Hegarmanah, Cibeusi, Cikeruh, Cipacing, Sayang, Cileles, Cilayung, Cisempur, Cintamulya, Jatimukti, Mekargalih, and Jatiroke in Jatinangor, Sumedang and golden snails identification was conducted at the laboratory. The sample was taken by using purposive sampling method. Each rice fields used as a sampling location has a size 10 m x 5 m with 2 rice fields were determined for sampling in each village. Therefore, total research location was 24 rice fields. Two lands were taken from one village with a distance of ± 1 km between the fields. This is done to get a comparison of environmental conditions. The results showed that the density of snails in the rice fields of Jatinangor was categorized rare with an average density of 3.33 individuals/m². The difference in the density of snails in each area in Jatinangor was caused by differences in the age/phase of rice plants and control techniques. Furthermore, distribution pattern of the golden snail at the rice fields of Jatinangor has a clumped pattern for all locations
Aplikasi Berbagai Jenis Pupuk terhadap Serangga Terrestrial dan Pertumbuhan Tanaman Padi Hitam Vira Kusuma Dewi; Reynaldi Reynaldi; Toto Sunarto; Santika Sari; Lindung Tri Puspasari; Rika Meliansyah
Soilrens Vol 21, No 1 (2023)
Publisher : Dept Ilmu Tanah & Sumberdaya Lahan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/soilrens.v21i1.49436

Abstract

Paddy field ecosystem is a complex ecosystem and build a food web. Tri-trophic interaction in paddy field involves rice plants as producers, phytophagous and neutral insects as first consumers, and their natural enemies as second consumers..This study was conducted to analyze the effect of siam weed and neem cake compost on abundance arthropod in black rice. The experiment was carried out in paddy field at Jatinangor from May to November 2018. The experimental method was used a randomized Complete Block Design with five treatment as follow neem cake, siam weed compost, cow manure, synthetic fertilizer and control) and six replications. The results showed, that arthropods abundance and plant performance including plant height, tiller number and SPAD value were higher in siam weed compost and neem cake treatment if compared with other treatments. Furthermore, there were strong correlation between insect herbivore with natural enemies in siam weed treatment.
Tipe Puru dan Serangga yang Berasosiasi pada Puru Daun Mangga (Mangifera indica L.) Varietas Gedong Gincu di Desa Karyamukti, Kecamatan Tomo, Kabupaten Sumedang Puspasari, Lindung Tri; Nurochman, Irvan Sopyan; Meliansyah, Rika; Dewi, Vira Kusuma; Bari, Ichsan Nurul; Qosim, Warid Ali
Agrikultura Vol 35, No 1 (2024): April, 2024
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/agrikultura.v35i1.54017

Abstract

Serangga pembentuk puru merupakan hama potensial yang dapat menurunkan hasil panen mangga dengan menghambat pertumbuhan bunga dan pembentukan buah pada tanaman. Informasi terkait serangga pembentuk puru serta parasitoidnya di Indonesia masih terbatas sehingga penting dipelajari jenis serta keragamannya dalam mendukung usaha pengendaliannya. Tujuan penelitian ini adalah untuk mempelajari jenis-jenis puru dan mengidentifikasi serangga pembentuk puru serta parasitoidnya yang berasosiasi pada puru daun mangga. Penelitian dilakukan dari bulan September 2022 hingga bulan April 2023 di Desa Karyamukti, Kecamatan Tomo, Kabupaten Sumedang.Pemeliharaan sampel daun mangga bergejala puru dilakukan di Laboratorium Bioteknologi Proteksi Tanaman, Departemen Hama dan Penyakit Tumbuhan, Fakultas Pertanian, Universitas Padjadjaran, Jatinangor. Identifikasi morfologi serangga dilakukan di Laboratorium Hama Tanaman, Departemen Hama dan Penyakit Tumbuhan, Fakultas Pertanian, Universitas Padjadjaran, Jatinangor. Sampel daun bergejala puru diambil secara purposive sampling pada 50 pohon mangga varietas Gedong Gincu kemudian dilakukan identifikasi morfologi pada serangga yang muncul dari daun bergejala puru tersebut. Hasil penelitian menunjukkan bahwa puru daun mangga yang ditemukan dapat dibedakan menjadi lima bentuk yaitu pustulate, low-doughnut, bell-shaped, conical, dan circular-blister. Serangga yang berasosiasi di dalamnya terkonfirmasi secara morfologi ada yang berperan sebagai pembentuk gejala puru yaitu Procontarinia robusta, beberapa parasitoid yaitu Chrysonotomyia sp., Pediobius sp., Mangostigmus sp., Eurytoma sp.1, Euryotoma sp.2, Platygaster sp., Eupelmus sp., dan Pteromalidae sp.1, serta satu serangga inquilines yaitu Phlaeothripidae sp.1. Hasil penelitian ini akan memberikan kontribusi penting terhadap pengetahuan dasar tentang pola serangan serangga di tanaman mangga dan merupakan data dasar untuk penerapan pengendalian hama.
Potency of Yeast’s Volatile Compounds to Control Cercospora coffeicola: Potency of Yeast’s Volatile Compounds to Control Cercospora coffeicola Hartati, Sri; Meliansyah, Rika; Mayanti, Tri
Jurnal Fitopatologi Indonesia Vol. 20 No. 1 (2024): Januari 2024
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.20.1.1-14

Abstract

Penyakit bercak daun cercospora yang disebabkan oleh Cercospora coffeicola merupakan salah satu penyakit utama pada tanaman kopi. Di antara metode alternatif untuk mengendalikan penyakit tanaman ialah dengan memanfaatkan senyawa volatil anticendawan yang dihasilkan oleh agens antagonis. Khamir merupakan salah satu agens yang berperan dan berpotensi menghasilkan senyawa volatil anticendawan. Penelitian ini bertujuan menentukan karakter morfologi khamir dan kemampuannya menghasilkan senyawa volatil yang bersifat anticendawan terhadap C. coffeicola. Isolasi khamir dilakukan dari tanaman kopi di beberapa lokasi di Kecamatan Cilengkrang, Kabupaten Bandung dan Kecamatan Jatinangor, Kabupaten Sumedang. Tahapan penelitian yang dilakukan terdiri atas isolasi khamir dari daun dan buah kopi, karakterisasi koloni dan sel khamir secara morfologi, dan uji kemampuan anticendawan senyawa volatil khamir secara in vitro dengan metode double dish system. Hasil penelitian didapatkan sebanyak 52 isolat khamir. Karakteristik koloni isolat khamir yang didapatkan didominasi oleh warna putih, krem, dan oranye, memiliki tepian yang rata, bergerigi hingga berfilamen serta memiliki bentuk koloni bulat hingga tidak beraturan. Karakteristik mikroskopis dari isolat khamir yang didapatkan menunjukkan bentuk yang bervariasi, yaitu bulat, bundar telur, jorong, bulat telur, serta memanjang dengan ukuran berkisar 2.59-18.13 × 1.23-7.77 µm. Seluruh isolat khamir yang didapatkan mampu menghambat pertumbuhan C. coffeicola melalui aktivitas senyawa volatil anticendawan dengan tingkat penghambatan berkisar antara 11.85% dan 79.26%.
Potensi Tiga Jenis Khamir dalam Mengendalikan Penyakit Bercak Cokelat (Alternaria solani Sorr.) pada Tomat Hartati, Sri; Istifadah, Noor; Aoliya, Salwa Rohmatul; Meliansyah, Rika; Mayanti, Tri
Agrikultura Vol 35, No 2 (2024): Agustus, 2024
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/agrikultura.v35i2.54374

Abstract

Penyakit bercak cokelat yang disebabkan oleh Alternaria solani Sorr. merupakan salah satu penyakit penting pada tanaman tomat yang dapat menyebabkan kehilangan hasil hingga 86%. Salah satu pengendalian yang dapat dilakukan untuk penyakit tersebut yaitu dengan agens biokontrol berupa khamir. Penelitian ini bertujuan untuk menguji potensi tiga spesies khamir dalam menghambat pertumbuhan A. solani secara in vitro dan menekan penyakit bercak cokelat pada tanaman serta buah tomat. Percobaan dilakukan di Laboratorium Bioteknologi Proteksi Tanaman dan Rumah Kaca Kebun Ciparanje, Fakultas Pertanian, Universitas Padjadjaran. Percobaan in vitro menggunakan Rancangan Acak Lengkap, sedangkan percobaan in vivo menggunakan Rancangan Acak Kelompok. Perlakuan pada pengujian in vitro dan in vivo berupa spesies khamir, terdiri dari Aureobasidium pullulans Dmg 11 DEP, Candida tropicalis Lm 13 BE, Rhodotorula minuta Dmg 16 BEP, fungisida berbahan aktif klorotalonil 75% (untuk pengujian in vivo), dan kontrol. Pengujian in vitro dilaksanakan dengan metode dual culture antara patogen A. solani versus khamir antagonis. Pengujian in vivo dilakukan dengan penyemprotan pada tanaman dan pencelupan buah tomat dalam suspensi sel khamir. Hasil percobaan in vitro menunjukkan bahwa khamir yang diuji dapat menghambat pertumbuhan koloni A. solani sebesar 23,67 % – 40,56%. Uji penekanan penyakit secara in vivo menunjukkan bahwa khamir yang diuji dapat menekan penyakit bercak cokelat sebesar 47,62% – 75,29% pada tanaman tomat dan 49,86% – 62,18% pada buah tomat. Khamir A. pullulans memberikan penekanan tertinggi, baik terhadap pertumbuhan koloni A. solani maupun terhadap penyakit bercak cokelat pada tanaman dan buah tomat.
The Ability of Three Species of Yeast in Inhibiting the in vitro Growth of Sclerotium rolfsii Sacc., the cause of damping off on soybean plants (Glycine max L.) Hartati, Sri; Setiani, Cahya; Meliansyah, Rika; Yulia, Endah; Mayanti, Tri
CROPSAVER Vol 7, No 2 (2024)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cropsaver.v7i2.58059

Abstract

Damping off disease caused by Sclerotium rolfsii is one of the important diseases of soybean plants. Biocontrol is considered as more environmentally friendly control method. Yeast is one of the biocontrol agents that can be used to control plant pathogens. This study was objected to test the potential of three species of yeast in inhibiting the growth of S. rolfsii. The experiment was arranged in the completely randomized design. The treatments were dual culture of the pathogen vs the yeasts on PDA and double dishes of S. rolfsii against three yeast isolates i.e. Rhodotorula minuta Dmg 16 BEP, Candida tropicalis Lm 13 BE, Aureobasidium pullulans Dmg 11 DEP, and a control. The results of the dual culture antagonism test showed that the three tested yeasts could inhibit the colony diameter of S. rolfsii by 23.30% – 40.00%, and the sclerotia formation by 46,33% – 98,05%. The results of the double dishes antagonism test showed that the three tested yeast isolates could inhibit the colony diameter of S. rolfsii by 19.60% – 28.20% and could inhibit 100% of sclerotia formation. The treatment of A. pullulans Dmg 11 DEP produced the highest inhibition in both the dual culture and double dishes antagonism tests.
The Ability of Nano Chitosan and Bacillus subtilis (Ehrenberg) Cohn to Suppress the Growth of Alternaria porri (Ellis) Ciferri Al Farisy, Muhammad Restu; Meliansyah, Rika; Susanto, Agus; Hersanti, Hersanti
CROPSAVER Vol 7, No 2 (2024)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cropsaver.v7i2.58185

Abstract

Purple blotch disease, caused by the fungus Alternaria porri (Ellis) Ciferri, poses a significant threat to shallot crops. Environmentally friendly control methods, such as applying nano-sized chitosan and Bacillus subtilis (Ehrenberg) Cohn, offer promising alternatives. This study aims to determine the effective concentrations of nano chitosan and B. subtilis, individually and in combination, to suppress the growth of A. porri in-vitro. The research was conducted from March to June 2024 at the Phytopathology Laboratory and Plant Protection Biotechnology Laboratory, Department of Pests and Plant Diseases, Faculty of Agriculture, Universitas Padjadjaran. A completely randomized design was employed, consisting of nine treatments with three replications each: a control treatment, B. subtilis 107 CFU/ml, 50 ppm nano chitosan, 100 ppm nano chitosan, 200 ppm nano chitosan, B. subtilis 107 CFU/ml + 50 ppm nano chitosan, B. subtilis 107 CFU/ml + 100 ppm nano chitosan, B. subtilis 107 CFU/ml + 200 ppm nano chitosan, and 80% mancozeb. The results indicated that nano chitosan at a concentration of 100 ppm was the most effective single treatment which could suppress the growth of A. porri colonies by 94% and inhibit conidia germination by 95%. Meanwhile, B. subtilis 107 CFU/ml + 100 ppm nano chitosan is the most effective mixed treatment which could suppress the growth of A. porri colonies by 84% and inhibit conidia germination by 90%.
Toxicity Effect of A Mixture Spiked Pepper (Piper aduncum L.) And Castor Bean (Ricinus communis L.) Against Crocidolomia pavonana F. Narendra, Nathanael Dimas; Dono, Danar; Meliansyah, Rika
CROPSAVER Vol 7, No 2 (2024)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cropsaver.v7i2.58910

Abstract

Crocidolomia pavonana is a cabbage crops pest that cause huge losses for farmers. Spiked pepper (Piper aduncum L.) and castor bean (Ricinus communis L.) extracts are known to be used as botanical insecticides because they have toxic compounds. This study aims to evaluate the toxic effects of a mixture of spiked pepper extract and castor oil as plant based insecticides against C. pavonana larvae and assess the index combination. The study consists of toxicity tests using experimental methods with a Randomized Complete Block Design with six treatments and four replications which were analyzed using analysis of probit regression and followed by an analysis of the mixture properties. Toxicity tests were conducted using a single spiked pepper extract, a single castor oil, and a mixture of spiked pepper extract and castor oil. The concentration of single spiked pepper extract used was 0.15%; 0.27%; 0.51%; 0.94%; 1.75%; and control, then the concentration of castor oil was 0.15%; 0.25%; 0.43%; 0.73%; 1.25%; and control, then the concentration of mixture of spiked pepper extract with castor oil was 0.30%; 0.41%; 0.57%; 0.79%; 1.1%; and control with a ratio 1:1. The results of the toxicity test showed that the mixture of spiked pepper extract with castor oil was toxic with LC50 and LC95 of 0.468% and 1.364% respectively at 10 days after treatment. The results of the mixture properties analysis showed that the mixture between spiked pepper and castor oil was additive at LC50 with a Combination Index value of 0.975 and was weakly synergistic at LC95 with a Combination Index value of 0.524.
PHENYLALANINE-INDUCED MODULATION OF CALLUS CHARACTERISTICS AND SECONDARY METABOLITE ACCUMULATION IN Ocimum basilicum L. UNDER IN VITRO CONDITIONS Suminar, Erni; Mubarok, Syariful; Budiarto, Rahmat; Yulianto, Fiky; Nuraini, Anne; Yuniarti, Anni; Kusumadewi, Vira; Meliansyah, Rika; Kurnia, Dikdik; Julaeha, Siti
Jurnal Agrotek Tropika Vol. 13 No. 3 (2025): JURNAL AGROTEK TROPIKA VOL 13, AGUSTUS 2025
Publisher : Departement of Agrotechnology, Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jat.v13i3.11044

Abstract

Basil (Ocimum basilicum L.) is widely used in traditional medicine due to its rich content of phenolic and flavonoid compounds. However, the natural production of these metabolites is limited. Callus culture offers a controlled method to enhance their accumulation, with medium composition, particularly precursor supplementation, playing a critical role. Phenylalanine, an aromatic amino acid, is a key precursor in the biosynthesis of phenolics and flavonoids via the shikimate pathway. This study aimed to evaluate the effect of phenylalanine on callus growth and the accumulation of total phenolic and flavonoid compounds in basil. The experiment was conducted at the Plant Tissue Culture Laboratory, Faculty of Agriculture, Universitas Padjadjaran, using Murashige and Skoog (MS) medium with phenylalanine concentrations of 0, 1.3, 1.6, and 2 g.L-1, each replicated six times. Results showed that phenylalanine treatments caused brown coloration, compact callus texture, and inhibited growth, as indicated by reduced fresh and dry weights. The 1.6 g.L-1 treatment produced the highest total phenolic content, while the 2 g.L-1 treatment yielded the highest flavonoid content. These findings suggest that phenylalanine can enhance secondary metabolite accumulation in basil callus, although it may suppress biomass growth.
Co-Authors Agus Susanto Al Farisy, Muhammad Restu Alghif Aruni Nur Rukman Anne Nuraini Anni Yuniarti Aoliya, Salwa Rohmatul Ceppy Nasahi Danar Dono Dikdik Kurnia Endah Yulia Erni Suminar Fitri Widiantini Hedi Paramita Hersanti - Ichsan Nurul Bari Ida Ayu Putu Sri Widnyani KIKIN HAMZAH MUTAQIN Kusumadewi, Vira Lindung Tri Puspasari Luciana Djaja - Luciana Djaya Martua Suhunan Sianipar Mukhamad Agung Yusuf Narendra, Nathanael Dimas Noor Istifadah Nurochman, Irvan Sopyan Paramita, Hedi R. Arif Malik Ramadhan Rahmat Budiarto Ramadhan Kamal Putra Rani Maharani Rani Maharani Reynaldi Reynaldi Rizky Ramdhani Santika Sari Setiani, Cahya Siti Julaeha, Siti SR HENDRASTUTI HIDAYAT Sri Hartati Sri Hartati Sri Hartati Sudarjat Sudarjat Syariful Mubarok Tarkus Suganda Teddy Budiyansyah Tiara Febricyanti Putri Senjaya Toto Sunarto Toto Sunarto - Tri Mayanti Unang Supratman Vira Kusuma Dewi Warid Ali Qosim Wawan Kurniawan Yulianto, Fiky Yusup Hidayat Yusup Hidayat