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All Journal e-Journal Pustaka Kesehatan Dentika Dental Journal Indonesian Journal of Cancer Chemoprevention STOMATOGNATIC- Jurnal Kedokteran Gigi Majalah Kedokteran Gigi Indonesia ODONTO Dental Journal Insisiva Dental Journal : Majalah Kedokteran Gigi Insisiva UNEJ e-Proceeding Dental Journal (Majalah Kedokteran Gigi) Padjadjaran Journal of Dentistry Journal of Dentomaxillofacial Science Medica Hospitalia Jurnal Karinov Journal of Community Development Odonto dental journal Dental Agromedis
Banun Kusumawardani
Department Of Biomedical Sciences, Faculty Of Dentistry, Universitas Jember, Jl. Kalimantan Tegalboto No.37, Jember 68121
Humanities Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Dentistry Economics, Econometrics & Finance Education Health Professions Immunology & microbiology Library & Information Science Medicine & Pharmacology Nursing Public Health Social Sciences

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Cytotoxic Potential of Flavonoid from Nicotiana tabacum Leaves on MCF-7 Human Breast Cancer Cells Kusumawardani, Banun; Febi, Qonita Nafilah; Rosidah, Malihatul; Azis, Deri Abdul; Puspitasari, Endah; Nugraha, Ari Satia
Indonesian Journal of Cancer Chemoprevention Vol 11, No 2 (2020)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev11iss2pp97-10

Abstract

Flavonoid has potential bioactivity as anticancer agents. The flavonoid of cultivated tobacco (Nicotiana tabacum), locally known as “Kasturi”, leaves was screened for its cytotoxicity against MCF-7 human breast cancer cells and non-transformed Vero cells (African normal cell kidney line) in different concentrations. This study aimed to examine the cytotoxic potential of the flavonoid of Kasturi tobacco leaves against MCF-7 human breast cancer cells. Flavonoid obtained from methanolic extracts of Kasturi tobacco leaves, which have been purified from nicotine. The flavonoid of Kasturi tobacco leaves with concentrations of 20 to 640 μg/mL were exposed to MCF-7 and Vero cells for 24 h. Cell viability was evaluated by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. Flavonoid of Kasturi tobacco leaves with concentrations of 160 μg/mL decreased the MCF-7 cell viability more than 50%, with an inhibitory concentration 50 (IC50) value of 148.41 μg/mL. Meanwhile, it inhibited 50% of Vero cell viability at 255.35 μg/mL. The flavonoid of Kasturi tobacco leaves has cytotoxic activity on MCF-7 cells, and might be a potential alternative agent for human breast cancer therapy.Keywords: flavonoid, tobacco leaves, human breast cancer cells, anticancer activity
The effect of supernatants of Lactobacillus casei against Porphyromonas gingivalis Zainina, Iris Amelinda; Kusumawardani, Banun; Sari, Desi Sandra
Majalah Kedokteran Gigi Indonesia Vol 11, No 2 (2025): August
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.101506

Abstract

Periodontitis is an inflammatory disease of the periodontal tissues primarily caused by Porphyromonas gingivalis (P. gingivalis). In recent years, probiotics have been proposed as a potential bacteriotherapeutic approach for periodontitis. Probiotic bacteria such as Lactobacillus casei (L. casei) are known to produce antibacterial compounds, which can be found in their culture supernatants. The ability of L. casei to grow and synthesize these substances depends on environmental factors, including pH. Based on preliminary findings, a pH of 6.5 was used in this study. This study aimed to evaluate the antibacterial activity of L. casei supernatant against P. gingivalis in vitro. The research employed an experimental post-test-only group design. Two control groups were included: a negative control (aquadest) and a positive control (0.2% chlorhexidine). The treatment groups received L. casei supernatant at volumes of 20 µl, 50 µl, and 80 µl, with incubation periods of 6, 12, and 24 hours. The results demonstrated that the L. casei supernatant exhibited the highest antibacterial activity against P. gingivalis at a volume of 80 µl after 12 hours of incubation. The Shapiro–Wilk test indicated that the data were normally distributed, while Levene’s test revealed a lack of homogeneity. Post hoc analysis showed significant differences among all treatment groups at the 12-hour incubation point. In conclusion, the supernatant of L. casei exhibits in vitro antibacterial activity against P. gingivalis.
THE CYTOTOXIC AND PROLIFERATIVE ACTIVITY OF COCOA POD HUSK EXTRACT (THEOBROMA CACAO L.) ON PERIODONTAL LIGAMENT FIBROBLASTS Rahayu, Yani Corvianindya; Triwahyuni, Iin Eliana; Kusumawardani, Banun; Sari, Dewi Yunita
Odonto : Dental Journal Vol 9, No 1 (2022): July 2022
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (394.479 KB) | DOI: 10.30659/odj.9.1.46-52

Abstract

Background: Theobroma cacao was considered agricultural wastes. Cocoa pod husk contain various bioactive compounds as natural antioxidants,antibacterial and anti-inflammatory. A good biomaterial must be non-toxic and have no detrimental effect on the biological environment. The aim of research was to determine the cytotoxicity and proliferative activity of cocoa pod husk extract on primary cells of periodontal ligament fibroblasts. Method: This experimental laboratory used primary cell culture of periodontal ligament fibroblasts. The cytotoxicity and proliferative activity were analyzed by MTT assay [2-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide]. Extraction of cacao pods were obtained using the ultrasonic homogenizer method with ethanol 70%. The cytotoxicity test was observed for 24 hours and the doubling time proliferative test was observed for 24, 48, and 72 hours. Result: Cocoa pod husk extract showed there were no cytotoxic effects with viability of primary cells of periodontal ligament fibroblasts at the concentration of 1.56 pg/ml, 3.125 pg/ml, 6.25 pg/ml, 12.5 pg/ml, 25 pg/ml, 50 pg/ml, and 100 pg/ml. The proliferation test showed increasing of fibroblasts at 24 and 48 hours. Kruskal Wallis analysis obtained a significance value (p<0.05) Conclusion: Cytotoxicity and proliferation assays of Cocoa pod husk extract increased cell viability and cell proliferation of periodontal ligament fibroblast.
THE CYTOTOXIC AND PROLIFERATIVE ACTIVITY OF COCOA POD HUSK EXTRACT (THEOBROMA CACAO L.) ON PERIODONTAL LIGAMENT FIBROBLASTS Rahayu, Yani Corvianindya; Triwahyuni, Iin Eliana; Kusumawardani, Banun; Sari, Dewi Yunita
Odonto : Dental Journal Vol 9, No 1 (2022): July 2022
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.9.1.46-52

Abstract

Background: Theobroma cacao was considered agricultural wastes. Cocoa pod husk contain various bioactive compounds as natural antioxidants,antibacterial and anti-inflammatory. A good biomaterial must be non-toxic and have no detrimental effect on the biological environment. The aim of research was to determine the cytotoxicity and proliferative activity of cocoa pod husk extract on primary cells of periodontal ligament fibroblasts. Method: This experimental laboratory used primary cell culture of periodontal ligament fibroblasts. The cytotoxicity and proliferative activity were analyzed by MTT assay [2-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide]. Extraction of cacao pods were obtained using the ultrasonic homogenizer method with ethanol 70%. The cytotoxicity test was observed for 24 hours and the doubling time proliferative test was observed for 24, 48, and 72 hours. Result: Cocoa pod husk extract showed there were no cytotoxic effects with viability of primary cells of periodontal ligament fibroblasts at the concentration of 1.56 pg/ml, 3.125 pg/ml, 6.25 pg/ml, 12.5 pg/ml, 25 pg/ml, 50 pg/ml, and 100 pg/ml. The proliferation test showed increasing of fibroblasts at 24 and 48 hours. Kruskal Wallis analysis obtained a significance value (p<0.05) Conclusion: Cytotoxicity and proliferation assays of Cocoa pod husk extract increased cell viability and cell proliferation of periodontal ligament fibroblast.
Penguatan Budaya Sadar Bencana Bagi Generasi Muda Desa Terdampak Erupsi Gunung Semeru di Desa Sumberwuluh, Kecamatan Candipuro, Kabupaten Lumajang Ali Badrudin; Banun Kusumawardani; Tecky Indriana; Agustina Dewi Setyari; Indra Nurtjahjaningtyas; Arif Mohammad Siddiq; Nindha Ayu Berlianti; Nilasari Dewi
Journal of Community Development Vol. 5 No. 3 (2025): April
Publisher : Indonesian Journal Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47134/comdev.v5i3.676

Abstract

Mount Semeru as one of the highest mountains on the Java island has significant volcanic activity to date. The eruption of this volcano had a considerable impact on the surrounding area, including Sumberwuluh Village, Candipuro District, Lumajang Regency. The impact felt by the community is not only physical damage but also trauma. Therefore, a disaster awareness culture needs to be built and strengthened early in order to reduce the impact caused when a disaster occurs. The Institute for Research and Community Service (LP2M) of the University of Jember in collaboration with the Regional Disaster Management Agency (BPBD) of Lumajang Regency carried out service activities for elementary school students and teachers in Sumberwuluh Village with the aim of strengthening the culture of disaster awareness. This activity has been attended by students and teachers from SDN Sumberwuluh 1, SDN Sumberwuluh 2, SDN Sumberwuluh 3, SDN Sumberwuluh 4, SDN Sumberwuluh 5 and MI Miftahul Ulum. This is carried out through two stages, socialization about the importance of disaster preparedness and direct practice of disaster preparedness consisting of water boat evacuation, webbing and trauma healing. This program has succeeded in increasing disaster preparedness awareness through disaster education from an early age. In addition, this activity also succeeded in improving knowledge and practical skills in disaster preparedness by 88% and building a better mentality and readiness in dealing with emergency situations.
Co-Authors Agustin Wulan Suci Dharmayanti Ali Badrudin Amara Kanya Maharan Ari Satia Nugraha Arif Mohammad Siddiq Azham Purwandhono Azis, Deri Abdul Budi Yuwono Budi Yuwono Dea Ajeng Pravita Suendi Dea Ajeng Pravita Suendi Depi Praharani Desi Sandra Sari Dewi Setyari, Agustina Dewi Yunita Sari Djaswadi Dasuki Djaswadi Dasuki Dwi Merry Christmarini Robin Dwi Merry Christmarini Robin Endah Puspitasari Febi, Qonita Nafilah Fitriyana, Nurul Isnaini Herniyati I Dewa Ayu Ratna Dewanti Iin Eliana Triwahyuni Indra Nurtjahjaningtyas Intan Julita Purnamasari Irma Josefina Savitri Izzata Barid Liesna Lusyana Nur Marsetyawan HNE. Soesatyo Marsetyawan Soesatyo Marsetyawan Soesatyo Muhammad Nurul Amin Nilasari Dewi Nindha Ayu Berlianti Nurud Diniyah Parawita Dewanti Riria Hendarto Putri Roedy Budirahardjo Rosidah, Malihatul Rudi Joelijanto Rusliyana Nuarita Rusliyana Nuarita, Rusliyana Suhartini Suhartini Suhartini Suhartini Sumono, Agus Syafiya Zata Yumni Tecky Indriana TRI HANDOYO Tsabitah Salsabil Aqilah Vananda Duanta Kantasa Wachju Subchan Widya Asmara Widya Asmara Yani Corvianindya Rahayu Yuliana Mahdiyah Daat Arina Zahara Meilawaty Zainina, Iris Amelinda