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All Journal ODONTO Dental Journal Insisiva Dental Journal : Majalah Kedokteran Gigi Insisiva Dental Journal (Majalah Kedokteran Gigi) Padjadjaran Journal of Dentistry Abdi Dosen : Jurnal Pengabdian Pada Masyarakat Jurnal Material Kedokteran Gigi Denta: Jurnal Kedokteran Gigi Odonto dental journal Indonesian Journal of Community Empowerment
Widyasri Prananingrum
Department Of Dental Materials, Faculty Of Dentistry, Universitas Hang Tuah, Surabaya, Indonesia
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Dentistry Education Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences

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Journal : Odonto dental journal

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The effect of effervescent acanthus ilicifolius l. extract on antifungal properties and flexural strength acrylic Widyasri Prananingrum; Fitria Rahmitasari; Dwi Andriani; Arifzan Razak
Odonto : Dental Journal Vol 9, No 2 (2022): December 2022
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (453.451 KB) | DOI: 10.30659/odj.9.2.310-318

Abstract

Background: Acanthus ilicifolius L. has antifungal potential and makes it possible to be a candidate for denture cleanser. This study aims to evaluate the effect of effervescent Acanthus ilicifolius L. extract on antifungal properties and flexural strength acrylic after immersion for three duration times.Method: Extracts were made by maceration method with ethanol. For the antifungal test, effervescent Acanthus ilicifolius L extract was prepared by combining the Acanthus ilicifolius L extract 4% and sodium perborate with two ratios, 1:7 and 1:14. The microdilution method was carried out. Effervescent Acanthus ilicifolius L extract 4% with ratio 1:7 was prepared for the flexural strength test. The fifty-four heat-cured acrylic resin (20x10x2.5mm) were fabricated and divided into 3 groups. Group 1 was immersed in aquadest (control); Group 2 was immersed in sodium perborate; Group 3 was immersed in effervescent Acanthous ilicifolius L. extract. Each group was immersed for 15 min, 8, and 56 hours (n=6). The flexural strength test was performed using Universal Testing Machine.Result: Effervescent Acanthus ilicifolius L. extract exhibited antifungal activity which increased in an effervescent Acanthus ilicifolius L extract-dependent manner significantly (0.57 to 0.50 OD). There were no significant differences between sodium perborate, effervescent_AI1 (1:7) and effervescent_AI2 (1:14). The flexural strength was found to be within the range of 66.1 - 68.3 MPa. There were no significant differences between groups (p < 0.05).Conclusion: Effervescent Acanthus ilicifolius L extract with the ratios 1:7 had a good antifungal effect and appropriate flexural strength after immersion for 15 min, 8, and 56 hours.
Viability of BHK-21 Fibroblast Cells Resulting from Synthesis of Collagen Powder (Thunnus albacares) with Variation of NaCl Concentration Zefanya, Kartika; Rahmitasari, Fitria; Widaningsih, Widaningsih; Prananingrum, Widyasri
Odonto : Dental Journal Vol 11, No 1 (2024): July 2024
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.11.1.94-104

Abstract

Background: Collagen plays a crucial role in post-extraction wound healing. Thunnus albacares skin, a collagen source synthesized through the papain soluble collagen (PaSC) method with varying NaCl concentrations, is assessed for non-toxicity using the MTT assay on BHK-21 fibroblast cell cultures. This research is to determine cell viability resulting from synthesizing collagen powder from the skin of yellowfin tuna (Thunnus albacares) with varying NaCl concentrations using the papain soluble collagen (PaSC) method. Method: Collagen powder was synthesized by cleaning Thunnus albacares skin measuring 1x1 cm, soaking it in 0.1 M NaOH, hydrolyzing it with CH3COOH, extracting it with papain enzyme, and then dividing the samples into four groups: without NaCl, NaCl 0.9 M, NaCl 1.3 M, and NaCl 1.7 M. Samples were centrifuged (6000 rpm) and followed by freeze-drying. Cell viability was obtained by conducting a cytotoxicity test using the MTT Assay method on BHK-21 fibroblast cells. Result: The percentage of cell viability in groups K, P1, P2, P3, and P4 were 100%, 10.708%, 113.750%, 107.833%, and 105.958%, respectively. The Kruskall-Wallis test yielded a significance value 0.000, indicating a significant difference (p<0,005). The Mann-Whitney test confirmed significant differences between groups. Conclusion: Collagen powder from Thunnus albacares skin with NaCl concentrations of 0.9 M, 1.3 M, and 1.7 M showed no toxic effects, while the group without NaCl showed toxic effects. Collagen powder with a NaCl concentration of 1.7 M yielded ideal results and showed no toxic effects.
The effect of incorporating hydroxyapatite into Type II glass ionomer cement on flexural strength and the examination of fractured surfaces using scanning electron microscopy Rahmitasari, Fitria; Cevanti, Twi Agnita; Prabowo, Puguh Bayu; Sularsih, Sularsih; Prananingrum, Widyasri
Odonto : Dental Journal Vol 10, No 2 (2023): December 2023
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.10.2.152-161

Abstract

Background: Glass ionomer cement (GIC) is widely recognized as the prevailing direct esthetic restorative material. In order for a restoration material to be considered effective, possess favorable physical and mechanical properties. Incorporating with hydroxyapatite (HA) can provide these properties. This research is to investigate the impact of integrating HA into GIC on the flexural strength and scanning electron microscopy (SEM) of the fractured surfaces. Methods: The study consisted of 28 samples of GIC, which are fabricated in the shape of rectangular prisms (25x2x2mm). The samples were divided into four groups n=7. Group 1 is a control group (GIC), Group 2 GIC+2%HA, group 3 GIC+2%HA, and GIC+4%HA. The flexural strength of the sample was evaluated by a Universal testing machine, followed by an examination of the fracture surface using SEM. Result: The Brown-Forsythe was used as data analysis to examine the flexural strength values across all groups, resulting in a statistically significant p-value of less than 0.05. The addition of HA does not result in a significant increase in the flexural strength value of the glass ionomer cement (GIC). The integration of microstructure based on scanning electron microscopy (SEM) pictures demonstrates improved visual quality following the incorporation of HA. Conclusion: The addition of HA to GIC did not result in a significant change in flexural strength compared to the control group. However, GIC group with 2% HA exhibited the highest average flexural strength value among all the groups
Cytotoxicity and mechanical properties of biphasic calcium phosphate scaffold from Tegilarca granosa due to its composition Prananingrum, Widyasri; Caroline, Grace; Rizal, Mohammad Basroni; Prabowo, Puguh Bayu; Pratama, Afif Fahwi; Resaldi, Muhammad Firdan; Annisa, Nindya Yuanita; Fadhilah, Yusti; Sari, Rima Parwati
Odonto : Dental Journal Vol 10, No 2 (2023): December 2023
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.10.2.247-256

Abstract

Background: Biphasic calcium phosphate (BCP) is graft material contained HA and TCP. Tegilarca granosa shell is a natural source that may converted into BCP. This study aims to determine the composition and cytotoxicity of BCP synthesized from Tegilarca granosa shell used various hydrothermal hours and to evaluate the mechanical properties of BCP scaffold. Methods: Tegilarca granosa shell was converted into BCP using hydrothermal method at 200˚C for 6h (Group 1); 9h (Group 2); and 12h (Group 3). The composition was determined by XRD and the cell viability were evaluated using MTT Assays. Each group was added with 20% gelatin ratio 50:50 (w/v) and freeze-dried to form scaffold. Scaffolds (Ø6mm x 4mm) were prepared for diametral tensile strength (DTS) test (n=6) and scaffolds (Ø7mm x 11mm) were used for compressive strength (CS) test (n=6). All data were analyzed using Kruskall-Wallis followed by Mann-Whitney test. Results: The composition of BCP (HA/ TCP) at Group 1, Group 2, and Group 3 were 81.80%/14,10%; 87%/6%; and 72%/21%. The cell viabilities were good for all groups. The DTS and CS test showed there was a significant difference between Group 1 and Group 3 scaffold, meanwhile there was no significant differences between Group 2 and Group 3 scaffold. Group 3 scaffold showed the highest DTS and CS, 6.921 MPa and 1,233 MPa. Conclusion: The BCP composition were depent on hydrothermal hours. Although all scaffold groups were non-toxic, but BCP scaffold synthesized from Tegilarca granosa shell using hydrothermal for 12 hours showed the highest mechanical properties.
Co-Authors Agustia, Lita Aisah Faiz Bachmid Akwan, Yulie Emilda Andriani, Dwi Annisa, Nindya Yuanita Arifzan Razak Ashrin, Meinar Nur Ayu R, Dyah Budi Teguh, Paulus Caroline, Grace Desak Putu Sudarmi Ari Dian Mulawarmanti, Dian Dian Widya Damaiyanti Diana Soesilo, Diana Dwi Andriani, Dwi Dwi Budi Santoso Dwi Setianingtyas Elidasari, Monica Elidasari, Monika Elizabeth Pranoto, Amelia Fadhilah, Yusti Fauzia, Bunga Firda Dean Yonatasya Firda Dean Yonatasya Fitria Rahmitasari Gita Saftiarini Hadinata, Yongki Hansen Kurniawan Hollanda, Ghita Hadi Indarti, Caecilia Karsini, Isidora Kharinna Widowati, Kharinna Kristanti Parisihni Kusumawardani, Chaterina Diyah Nanik Laihad, Fanny M. Lestari, IGAM Oka Linda Rochyani, Linda Lizda Johar Mawarani Meinar Nur Ashrin Moh. Basroni Rizal Nafi'ah Pambudi Rahardjo Parasihni, Kristanti Pargaputri, Agni Febrina Prabowo, Puguh Bayu Pratama, Afif Fahwi Priambodo, Nur Tsurayya Priska Evita Setia Dharmayanti Puguh Bayu Prabowo, Puguh Bayu Putri, Vindya Meinda Putu Tahlia Krisna Dewi Rahim, Muhammad Galang Adhinata Abdul Ramadhania, Nurina Rizka Resaldi, Muhammad Firdan Retno Pudji Rahayu Reyhan Alvaryan Ferdynanto Rima Parwati Sari, Rima Parwati Rizal, Mohammad Basroni Rizka, Yoifah Saptaswari, Dianty sari, Rima parwati Setiawan, Grace Caroline Son, Meekyoung SRI FATMAWATI Sularsih, S Syamsulina Revianti Tameno, Yolanda Agustina Tinisia, Adelia Tsurayya, Nur Twi Agnita Cevanti Wahyuning, Baiq Nadia Risna Widaningsih Widaningsih, Widaningsih Yessy Andriani Fauziah Yonatasya, Firda Dean Yulie Emilda, Yulie Zefanya, Kartika