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Mokosuli Yermia Samuel
Universitas Negeri Manado
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Public Health Social Sciences Veterinary Other

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Journal : Jurnal Pembelajaran dan Biologi Nukleus

 1 
Bioactive Contents, BSLT Toxicity and antioxidants from Apis dorsata Binghami and Apis mellifera nest extracts Tuegeh, Reinny Silvana; Sumampouw, Herry Maurits; Tanor, Meity Neltje; Mokosuli, Yermia Semuel
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 9, No 2: Jurnal Pembelajaran Dan Biologi Nukleus July 2023
Publisher : Fakultas Keguruan dan Ilmu Pendidikan Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v9i2.4514

Abstract

Beenest is rich in secondary metabolites because honeycombs, among others, are formed from plant resins (propolis). This study aimed to analyze differences in flavonoid content and in vitro antioxidant activity of Apis dorsata Binghami and Apis mellifera nest extracts. The samples used were A. dorsata from North Sulawesi and A. mellifera from South Sulawesi. The honeycomb was extracted using 95% ethanol solvent. Honeycomb extract was analyzed for its flavonoid content by the HPLC method, toxicity was tested by the BSLT method, and in vitro antioxidant activity was tested by the DPPH method. The results of the analysis of flavonoids showed that A. dorsata nest extract produced 21 types of compounds while A. mellifera produced 26 types of compounds. The toxicity test results showed that the A. dorsata nest extract had a better LC50 of 245,691 mg/l than the A. mellifera nest extract with an LC50 of 443,701 mg/l. The in vitro antioxidant test results of A. dorsata nest extract were more robust, namely IC 50 1.161 mg/l, compared to A. mellifera nest extract IC 50 2.404 mg/l. However, both were included in the category of powerful antioxidants. In vitro, anticancer test results on MCF-7 cells, A. mellifera nest extract was active with IC 50 100.02mg/l. Compared to A. dorsata extract, it was active with IC 50 102.217mg/l, but the two were not significantly different. Based on the analysis of flavonoid content, toxicity test, and antioxidant test, A.dorsata and A.mellifera honeycomb extracts have potential as in vitro antioxidants
The Nutritional Content Found in Rhizophora Mangrove Fruits in Sondaken Village, Tatapaan Subdistrict, South Minahasa Mongdong, Priskila Indah Theresya; Mokosuli, Yermia S.; Ogi, Nova L.I.M
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 9, No 3: Jurnal Pembelajaran Dan Biologi Nukleus November 2023
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v9i3.4468

Abstract

Mangroves are fertile plants that dominate the coastal areas. The fruit of the Rhizophora mangrove can be used as food and beverage ingredients, containing numerous nutrients for the human body. The mangrove fruit contains carbohydrates, water, protein, fat, as well as secondary metabolites such as flavonoids, triterpenoids, tannins, and saponins. The aim of this research is to determine the nutritional content of the Rhizophora apiculata, Rhizophora mucronata, and Rhizophora stylosa mangrove fruits in Sondaken Village, Tatapaan Subdistrict, South Minahasa. This study employs a descriptive research method, where laboratory experiments were conducted using the SNI 01-2891-1992 method at the Baristand Manado laboratory. The nutritional analysis results for Rhizophora apiculata showed a water content of 62.17%, ash content of 1.09%, protein content of 0.59%, fat content of 0.79%, crude fiber content of 5.45%, and carbohydrate content of 35.36%. For Rhizophora mucronata, the analysis revealed a water content of 65.54%, ash content of 3.07%, protein content of 1.27%, fat content of 0.74%, crude fiber content of 7.52%, and carbohydrate content of 29.38%. Lastly, Rhizophora stylosa had a water content of 64.77%, ash content of 3.547%, protein content of 1.30%, fat content of 1.63%, crude fiber content of 7.14%, and carbohydrate content of 28.76%. These results indicate that mangrove fruits have numerous benefits and contain valuable nutrients and good nutritional value for the body. By preserving the mangrove ecosystem and utilizing the nutrients found in the fruits and leaves, there is an opportunity to explore their potential as alternative natural remedies
Isolation, Identification, and Antibacterial Assay of Indigenous Bacterial Isolates from Apis cerana Honeycomb Simbolon, Kristina Br; Mokosuli, Yermia; Lawalata, Helen J
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 9, No 1: Jurnal Pembelajaran Dan Biologi Nukleus March 2023
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v9i1.3441

Abstract

Apis cerana is a honey bee species with a cavity hive known as the eastern honey bee. Honey bee hives have Indigenous bacteria that have antimicrobial potential. Indigenous bacteria are free bacteria that can synthesize nitrogen compounds, sugars, and other bioactive substances. This study aims to isolate, identify and test the antibacterial of Indigenous bacteria isolate Apis cerana against gram-positive Staphylococus aureus and gram-negative bacteria Escherichia coli. This study used a descriptive research method to obtain the data from laboratory experiments. The study consisted of isolating Indigenous bacteria, identifying and testing the antibacterial agar diffusion method. The study results obtained seven isolates of AC 1, AC 2, AC 3, AC 4, AC 5, AC 6, AC 7 Indigenous bacteria from Apis cerana nests. AC 1 isolate has similarities with the genus Streptococcus, AC 2 isolates have similarities with the Klebsiella genus, while AC 3 isolates have similarities with the Bacillus genus, and AC 4, AC 5, AC 6 and AC 7 isolates have similarities with the Citrobacter genus. Indigenous bacterial isolates with potential antibacterial potential where the most significant inhibition against Staphylococus aureus bacteria was shown by isolate AC 1 (10.79 mm). At the same time, the smallest was found in isolate AC 7 (8.52 mm). The most significant inhibition against Escherichia coli bacteria was shown by isolate AC 1 (9.0 mm) while the smallest was found in isolate AC 3 (7.4 mm). Apis cerana nests have indigenous bacteria that have the potential to produce antibacterial substances
Antagonistic and Antibacterial Activity of Stapylocossus aureus and Isolates of Oral Bacteria from the Endogenous Fungus Apis dorsata Binghami Nest Semuel, Mokosuli Yermia; Wurarah, Masje; Tuegeh, Reinny Silvana
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 8, No 2: Jurnal Pembelajaran Dan Biologi Nukleus Juli 2022
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v8i2.2739

Abstract

Apis Dorsata Binghami honey bee is a honey bee endemic to Indonesia, living naturally in the forests of Sulawesi. This study aimed to obtain isolates and characteristics of endogenous fungi antibiotic activity from Apis dorsata Binghami nest. The research consisted of isolation of fungi from beehives using potato dextro agar, pure culture of fungi, antagonist test and antibiotic test using disc diffusion method. Antibiotic test was performed on oral bacteria and Staphylococcus aureus. The results obtained six isolates of fungi from the nest of Apis dorsata Binghami, namely isolates FAB1, FAB2, FAB3, FAB4, FAB5 and FAB6. The results of the antagonist test showed that the isolates FAB2 and FAB3 had the best antagonist properties, while the FAB6 isolates had the weakest antagonist properties. The FAB2 isolate showed the best bacterial growth inhibition zone average for the isolates of oral bacteria and Staphylococcus aureus. The inhibition zone lasts up to 3 x 24 hours so that the activity of the bacteria is bactericidal. From the results of this study, it can be concluded that the endogenous fungus Apis dorsata Binghami is a potential source of antibacterial bioactives.
In Vitro Antidiabetic Activity and Bioactive Ingredients of Apis mellifera and Apis dorsata Binghami Nest Extracts Sibala, Henike; Naharia, Orbanus; Manampiring, Nonny; Mokosuli, Yermia Semuel
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 9, No 2: Jurnal Pembelajaran Dan Biologi Nukleus July 2023
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v9i2.4511

Abstract

Indonesia ranks 7th out of ten countries with diabetes mellitus, which continues to increase yearly. Honeycomb is rich in secondary metabolites formed from plant resins (propolis) which contain alkaloids, flavonoids, saponins, tannins, steroids and triterpenes. The research aimed to analyze the comparison of flavonoid content and antidiabetic activity with the enzyme α-glucosidase In Vitro nest extract of A. mellifera and A. dorsata Binghami. The samples used were nests of A. mellifera from northern Toraja, southern Sulawesi and A. dorsata Binghami from Southeast Minahasa, North Sulawesi. Using 96% ethanol, HPLC method of flavonoid testing and in vitro antidiabetic activity testing using α-glucosidase enzymes. The flavonoid content results were based on the number of peaks produced and the retention time of A. mellifera produced 26 compounds, and A. dorsata Bingham produced 21 compounds. The results of in vitro antidiabetic tests with α-glucoside enzyme inhibitors obtained IC50 values from the nest extract of A. mellifera of 3.605 mg/L and the nest extract of A. dorsata Binghami of 4.992 mg/L. The extract from A. mellifera had better antidiabetic potential than that of A. dorsata Binghami, although the difference is insignificant.
In Vitro Antidiabetic Activity Of Apis dorsata Binghami Nest Extract Pratasik, Veronika; Mege, Revolson; Yermia, Mokosuli Semuel
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 8, No 3: Jurnal Pembelajaran Dan Biologi Nukleus November 2022
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v8i3.3196

Abstract

Indonesia is ranked 7th out of 10 countries with the highest number of people with diabetes mellitus, which is estimated to increase every year.α -amylase is an enzyme that catalyzes the hydrolysis of α-1,4 glycosidic bonds of polysaccharides to produce dextrins, oligosaccharides, maltose and D-glucose. Apis dorsata Binghami honeycomb contains secondary metabolites that have antidiabetic potential, including inhibiting the action of the α-amylase enzyme. The purpose of this study was to determine the in vitro antidiabetic potential of Apis dorsata binghami nest extract. This study uses a descriptive research method where the research data are obtained through laboratory experiments. Honeycomb extraction using maceration method. Analysis of total flavonoid content using UV-Vis spectrophotometric method. Antidiabetic potency test using -amylase enzyme inhibition method. The results of the extraction study showed that the % yield of the extract was 11.82% with a brownish yellow color. Analysis of the total flavonoid content showed the results of 3.33 mgQE/g. The inhibitory activity of the -amylase enzyme, the IC50 value of the extract obtained was 158.48±7.42 g/mL, the acarbose value was 165.96±7.08 g/mL. These results indicate that the extract of A.dorsata Binghami nest has hyperglycemic activity by inhibiting complex carbohydrate hydrolyzing enzymes such as inhibition of the -amylase enzyme which is better than acarbose because the IC50 value of the extract is smaller. If the IC50 value is smaller then the enzyme inhibition is stronger. For further research, it is recommended to use a more specific enzyme, namely the -glucosidase enzyme.
Co-Authors Alfonds Andrew Maramis Anice I. Tuda Anita C. C. Tengker Arrijani - Budiarto Buyang, Dalle Carolin Manuahe Decky D. W. Kamagi Dewa Nyoman Oka Dina Munawaroh Dina Rombot Djaila, Friscila Avianti Edieli Zebua Edyson Maneasa Emma M Moko Esibrena Br Kemit Eurika, Uwuh Jessie Fadly Steven Jefry Rumondor Fanny Nella Nanlohy Febiola Gabriela Tular Febiola Najoan Ferencia Esananda Rattu Ferni Margo Tumbel Ferny Margo Tumbel Feydina Tewu Friska Mery Montolalu Futwembun, Fransiska Gedoan, Sukmarayu Piter Grace Jenny Soputan H. M. Sumampouw Hariyadi Hariyadi Helen J Lawalata Helen J Lawalata Helen Joan Lawalata Hendra Pratama Maliangkay Hermanus, Britney Putri Herry Maurits Sumampouw Ilham Saputra Butar-butar IRIANI SETYAWATI Iriani Setyawati Jacklin Stella Manopo Jantje Ngangi Jantje Pelealu Jeane Mantiri Jimmy Langi Jonathan Polandos julduz ruland paus Kamagi, Decky D. W. Kandowangko, Sandy Kaunang, Eva S. N. Maidangkay, Vonny Mamuaya, Tripena Manampiring, Noni Manampiring, Nonny Mansyur, Djailani Masje Wurarah Masje Wurarah Masye Wurarah Max Tulung Meike Paat Meisa Tabita Rogahang Meity Nelltje Tanor Melita Irene Gracia Kaligis Meyer Worang Matey Migau, Natalina Mongdong, Priskila Indah Theresya Motulo, Serlin J Mulder, Keysia Stephanie Tesalonica Mundaeng, Camel Gantonio Nerni Potolangi Nonny Manampiring Nova L.I.M Ogi Ogi, Nova L I M Ogi, Nova L.I.M. Orbanus Naharia Orbanus Naharia Pomalingo, Moh Fikri Pratasik, Stralen Pratasik, Veronika Pua, Devis Christian Rahardiyan, Dino Revolson Mege Rolef Rumondor Rudi Alexander Repi Rymond Jusuf Rumampuk Sibala, Henike Simbolon, Kristina Br Sinyo Herry Sumampouw Suddin Simandjuntak Sukmarayu Gedoan Sukmarayu P. Gedoan Sumampouw, Harry Maurits Taariwuan, Marlin Bernadet Tengker, Anita Costanci Christine Thesia Megi Kurniawati Rettob Timbuleng, Nobel Tjiakra, Rosalina A. Tuegeh, Reinny Silvana Tumanduk, Yahya Y.R. Turambi, James S. Utari Satiman Verawati I Y Roring Verawati Ida Yani Roring Verra E. J. Mawitjere Wisda Nurjannah Abdullah Worang, Rina L. Wuwungan, Axel Yessie K. Kelly