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Mokosuli Yermia Samuel
Universitas Negeri Manado
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Public Health Social Sciences Veterinary Other

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Journal : Indonesian Biodiversity Journal

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Characteristics Of Partial Mithchondrial Cytochrome Oxidase Sub Unit 1 Gene Of Honey Bee Apis dorsata Binghami [Hymenoptera: Apidae] From Minahasa, North Sulawesi, Indonesia: Characteristics Of Partial Mithchondrial Cytochrome Oxidase Sub Unit 1 Gene Of Honey Bee Apis dorsata Binghami [Hymenoptera: Apidae] From Minahasa, North Sulawesi, Indonesia Yermia Semuel Mokosuli; Revolson Alexius Mege; Jantje Pelealu; Max Tulung
Indonesian Biodiversity Journal Vol. 1 No. 1 (2020): April 2020
Publisher : Universitas Negeri Manado

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The aims of this study were to characterize the mitochondrial cytochrome oxidase subunit 1 gene (CO1) of Apis dorsata from Minahasa, North Sulawesi. Apis dorsata Binghami used in this study was obtained from the natural colony in Klabat forest, Airmadi forest and Kombi forest. From each native bee colony, ten workers were taken (n = 30). Femur hind legs used for subsequent DNA extraction were amplified by PCR, underwent electrophoresis and then sequenced. CO1 sequences then were matched with BLAST to obtain sequences from the NCBI that have high levels of homology (>90%). The results showed that the universal primer LCO -1490 and HCO - 2198 success amplify the gene CO1 Apis dorsata of Minahasa. Based on the phylogeny tree, Apis dorsata derived from forest Airmadidi and forest Kombi have the same CO1 sequences but have had differences with Apis dorsata from forests Klabat. BLAST analysis results showed that the partial mitochondrial CO1 sequences Apis dorsata of Minahasa has the highest rate of 94 % similarity with similar sequences recorded in the NCBI gene bank.
Aedes Mosquito Population density of Dengue Fever Vectors in The Area of Pineleng Minahasa: Aedes Mosquito Population density of Dengue Fever Vectors in The Area of Pineleng Minahasa Carolin Manuahe; Yermia Semuel Mokosuli; Ferny Margo Tumbel
Indonesian Biodiversity Journal Vol. 1 No. 1 (2020): April 2020
Publisher : Universitas Negeri Manado

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Aedes mosquitoes have the behavior of sucking blood repeatedly (multiple bites) in one gonotrophic cycle, these mosquitoes are also very effective at transmitting the virus to humans (WHO 2004). Kawada et al. (2007) reported that the foraging activity of A. albopictus was less than 0.1 times compared to A. aegypti under laboratory conditions, this result is consistent with the tendency to bite both species in nature. The target-attack frequency (foraging activity) in A. aegypti female mosquitoes in unfed conditions is 30 times greater than that of A. albopictus mosquitoes in several laboratory treatments (Kawada, et al. 2007). Aedes sp is a deadly infectious vector between another dengue hemorrhagic fever (DHF) and chikungunya. Pineleng is a marginal/suburban area of Station 1. Recent studies have reported changes in habitat patterns of Aedes sp, especially Aedes albopictus and Aedes aegypti . The aim of this study is to characterize the population density of the Aagoan sp. Imago based on the time distribution and altitude of the sea surface. The population density characteristics of the Aedes spagoed imago captured inside and outside the room based on the time distribution in North Sulawesi. The stages of the study consisted of larvae surveying, larval extraction and analysis, and imago extraction and analysis. The results showed that the high population of Ae aegypti was at 8:00 a.m. to 10:00 p.m. in the morning and 14:00 to 16:00 p.m. in the afternoon before the afternoon. in the morning before noon and at 14:00 to 18:00 in the afternoon before evening.
Nutmeg (Myristica fragrans) Of North Minahasa Molecular Entication Based on chloroplast DNA of matK gene Fanny Nella Nanlohy; Yermia Semuel Mokosuli
Indonesian Biodiversity Journal Vol. 1 No. 1 (2020): April 2020
Publisher : Universitas Negeri Manado

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Plant Nutmeg (Myristica fragrans) is an endemic plant in Indonesia originating from Banda Maluku Province. Nutmegs have long been known to people as spices. The nutmeg plants have been cultivated in North Sulawesi for a long time. This research has been conducted to obtain the position of species and the reconstruction of the phylogenetic tree of nutmeg plant (Myristica fragrans) from North Minahasa based on chloroplast DNA of matK gene. This research uses a descriptive method with a laboratory experiment. Total DNA was obtained by leaf tissue extraction and purification. The matK gene was amplified using the PCR method and visualized with automatic electrophoresis, sequencing was performed at FIRST BASE Singapore. The result of the analysis of the gene sequence of North Min Minerals using Barcode Of Life Database (BOLD) Systems shows that Pala Wori and Pala Patokaan have 99% similarity with Myristica maingayi and Myristica glubosa. While the reconstruction of the phylogeny tree shows the North Minahasa Pala has the closest resemblance to Myristica fatua.
Application Of Apis dorsata Binghami Bee Nest Extract On The Effects Of Human Blood Lipid Reduction Dina Munawaroh; Yermia S Mokosuli; Anita C. C. Tengker
Indonesian Biodiversity Journal Vol. 2 No. 2 (2021): Agustus 2021
Publisher : Universitas Negeri Manado

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Honey, royal jelly, bee pollen, bee wax, propolis, bee glue, and bee venom/ apitoxin are among the items produced by honey bees. Honey, pollen, bees, eggs, larvae, and pupae are all stored in the A. dorsata Binghami beehive. Secondary metabolites and bioactive phenolic compounds (phenols, phenolic acids, esters, flavors, dihydroflavones, flavanols, chalcones, and phenolic glycerides), essential oils (mono and sesquiterpenes), polysaccharides, amino acids, amines, lactones, quinones, steroids, and vitamins) can all be found in honeycomb. The goal of this study is to see how A. dorsata Binghami honeycomb tea affects blood lipid levels in humans after 14 days of consumption in a small group of male and female volunteers who were given a 2 gram tea bag extract of A. dorsata Binghami honeycomb simplicia to drink once a day to see how it affects lipid levels (total cholesterol, LDL, HDL, and TAG) in human blood. Total cholesterol (140.56 mg/dL), triglycerides (140.93 mg/dL), LDL (85.92 mg/dL), and HDL (21.49 mg/dL) were all found to be high in humans. As a result, "The Use of A. dorsata Binghami's Honeycomb Extract on the Effect of Reducing Human Blood Lipids" can lower lipid levels.
THE BIOACTIVE CONTENT AND ANTIOXIDANT ACTIVITY OF HONEY BEEHIVES (Apis dorsata BINGHAMI) FROM THE BOGANI NANI WARTABONE BOLAANG MONGONDOW NATIONAL PLANT Yermia S Mokosuli; Herry Sumampouw; Hendra Pratama Maliangkay; Jimmy Langi
Indonesian Biodiversity Journal Vol. 2 No. 1 (2021): April 2021
Publisher : Universitas Negeri Manado

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Bee Honey (Apis dorsata Binghami) Is a subspecies of A. dorsata which is only found in sulawesidan island and surrounding islands and until now has not been successfully cultivated. Various studies have been conducted species of trees used for nesting places, nesting behavior, biotic conditions, physical surrounding nesting trees and nesting characteristics. This study aims to obtain secondary metabolite components and scientific data of antioxidant activity of honeybee extract (Apis dorsata Binghami) derived from Bogani Nani Wartabone Bolaang Mongondow National Park, North Sulawesi Province. The method of extraction in this research was conducted using maceration with methanol solvent, phytochemical test and antioxidant activity analysis using method (1,1-definil-2-picrilhidrazil) by measuring absorbance absorption at maximum wavelength using UV-Vis spectrophotometer. The results of the phytochemical test show that the honeycomb (Apis dorsata Binghami) contains alkaloids, flavonoids, saponins and tannins. From the analysis results showed that with the increasing concentration of extracts, the greater the antioxidant activity is marked by decreasing the value of IC50. The honeycomb (Apis dorsata Binghami) has IC50 of 212.42 ppm in comparison with vitamin C IC50 of 59.34 ppm
BIOAKTIVASI LARVASIDA NYAMUK Aedes sp. DARIiEKSTRAK DAUNiECENGiGONDOK (Eichhornia crassipes (Mart.) Solms) DARI DANAUiTONDANO Jonathan Polandos; Revolson Alexius Mege; Yermia Mokosuli
Indonesian Biodiversity Journal Vol. 3 No. 1 (2022): April 2022
Publisher : Universitas Negeri Manado

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Nyamuk Aedes sp. adalah vektor utama penyakit demam berdarah dengue yang mempunyai kiprah besar pada terhadap penyebaran penyakit DBD di Indonesia. Pengendalian penyebaran vektor nyamuk dibutuhkan agar dapat menurunkan populasi vektor nyamuk Aedes sp. sehingga dapat menurukan masalah penyebaran DBD. Salah satu pengendalian vektor penyakit DBD yaitu mengunakan larvasida yang berasal dari tumbuhan. Ekstrak alkohol daun eceng gondok (Eichhornia crassipes (Mart.) Solms) diduga memiliki pengaruh larvasida Aedes sp., sebab mempunyai kandunan flavonoid, saponin dan tanin yang mengganggu perkembanan larva nyamuk Aedes sp. Tujuan dari penelitian ini untuk mengetahui efetivitas ekstrak (crude extract) alkohol 70% daun eceng gondok (Eichhornia crassipes (Mart.) Solms) terhadap mortalitas larva nyamuk Aedes sp. Penelitian eksperimental dilakukan dengan subyek dari penelitian yaitu 150 larva nyamuk Aedes sp. instar 3. Subyek penelitian dibagi menjadi lima grup pelakuan, konsentrasi kandungan yang digunakan di penelitian adalah 1000 ppm, 1500ppm, 2000 ppm, 2500ppm dan untuk kontrol positif adalah abate 1%. Pengamatan dilakukan 1x24 jam di ke 24 jam setelah perlakuan, perlakuan dilakukan replikasi sebanyak 3 kali. Data dianlisis serta dilakukan penghitungan nilai Lethal consentration mengunakan analisis probit. Berdasarkan analisis probit tidak ditemukan nilai dari LC50 di seluruh konsentrasi. Ekstrak (crude extract) alkohol 70% daun eceng gondok (Eichhornia crassipes (Mart.) Solms) tidak mempunyai efek larvasida nyamuk Aedes sp. Kesimpulan ekstrak (crude extract) alkohol 70% Daun eceng gondok (Eichhornia crassipes (Mart.) Solms) tidak mampu membunuh nyamuk vektor penyakit DBD nyamuk Aedes sp. di seluruh tingkat konsentrasi sesudah dilakukan pengamatan selama 24 jam.
CORRELATION BETWEEN BODY WEIGHT AND ORGAN WEIGHT IN DIGESTIVE SYSTEM OF NORTH SULAWESI LOCAL PIG Revolson Alexius Mege; Nonny Manampiring; Yermia Semuel Mokosuli
Indonesian Biodiversity Journal Vol. 2 No. 3 (2021): Desember 2021
Publisher : Universitas Negeri Manado

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North Sulawesi local pigs have good potential because they have a high adaptability to the environment and low-quality feed obtained from processed food remnants of the population. The samples used in this study were local sows from North Sulawesi which were spread on traditional farms. The purpose of this study was to obtain data on body weight of livestock including the weight of organs in the digestive system (stomach, intestine and liver). Body weight data will be correlated with organ weight and intestinal length data. The results showed that the average body weight of the local pigs used in this study ranged from 16.65 ± 0.66 kg with the age of the cattle ranging from 1 year. The results of statistical analysis of the correlation test between body weight and organ weight of local pigs showed a very significant correlation value (P < 0.01). The correlation value shown in the study shows a very strong value in the correlation between body weight and the weight of an empty stomach and a strong correlation value is shown in the correlation between body weight and liver weight and intestine length, meaning that the weight of organs in the body in the digestive system will increase with increase in body weight. Body weight is one indicator of livestock productivity that can be estimated based on the size of the animal's body.
IDENTIFICATION AND CHARACTERIZATION OF FEED PLANTS FOR Apis dorsata Binghami IN THE PINABETENGAN FOREST, MINAHASA Feydina Tewu; Orbanus Naharia; Yermia Mokosuli
Indonesian Biodiversity Journal Vol. 4 No. 2 (2023): August 2023
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v4i2.6023

Abstract

Apis Dorsata Binghami is a honey bee that is often found in the forest, which is conserved by the community, but there are also honey bees that are just left alone; the community preserves these honey bees because their honey can be traded and can also be used as medicine. This study aims to determine the plants that are a feed source for Apis Dorsata Binghami in the Pinabetengan Forest, Minahasa Regency. This research was conducted in June-August 2022. The data collected came from field survey data using descriptive methods and identified plant species with values of Reactive Density (KR), Relative Frequency (FR), Relative Dominance (DR), and Index Significant Value (INP). Plant diversity as a food source for Apis Dorsata Binghami has different types of plants based on their habitus or the characteristics of the plants themselves.
ANALYSIS OF MANGROVE FOREST VEGETATION ON MOLAS BEACH, BUNAKEN DISTRICT, MANADO CITY Futwembun, Fransiska; Lawalata, Helen J; Setyawati, Iriani; Ogi, Nova L I M; Manampiring, Nonny; Mokosuli, Yermia
Indonesian Biodiversity Journal Vol. 5 No. 2 (2024): August 2024
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v5i2.10753

Abstract

The lack of scientific information about the current condition of the mangrove ecosystem on the Molas beach in the Bunaken District of Manado City is an important reason to conduct a study related to the analysis of mangrove forest vegetation. The purpose of this research is to identify the types of mangroves and to find the Important Value Index (INP) of mangroves on the Molas beach in the Bunaken District of Manado City. Data collection was conducted using the line transect method with plots at only one observation station, which was then analyzed to determine the Importance Value Index (INP). Based on the observations, it was found that four types of mangroves were present at the research location. The dominant mangrove species are Bruguiera Cylindrica, Bruguiera gymnorrhiza, Aegiceras cosniculatum, and Ceriops tagal. The substrate condition, which is dominated by muddy sand, and the water quality parameters that affect mangrove growth generally still meet the standards for mangrove growth. The species Ceriops tagal has the highest values of Relative Density, Relative Frequency, and Relative Dominance, resulting in the highest INP. Meanwhile, the lowest INP was found in the species Bruguiera gymnorrhiza in the tree category
CHARACTERIZATION OF SAPROPHAGE INSECTS FORENSIC ENTOMOLOGY INDICATORS USING LOCAL PIGS Kandowangko, Sandy; Wurarah, Masje; Mokosuli, Yermia; Satiman, Utari; Gedoan, Sukmarayu
Indonesian Biodiversity Journal Vol. 5 No. 2 (2024): August 2024
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v5i2.10755

Abstract

Forensic entomology is the science of insects which is used to analyze cases related to forensics, namely death. This is based on the close relationship between humans and insects who live side by side. Insects can be a factor in determining the Postmortem Interval (PMI) so that it is useful in the forensic investigation process. Medicolegal insects are insects that are used as evidence in death cases. This research aims to obtain the diversity of insect species, the morphological characteristics of insects, the characteristics of insects in experimental pigs that died from hanging, poisoning and stabbing and based on the stage of decomposition. The results of this research examine temperature, soil pH, air humidity and the decomposition process of local pigs. The results of research on the decomposition stage in 3 deaths (hanging, stabbing, and poisoning) showed differences in the time of death, where the hanging carcass decomposed more quickly than the stabbed and poisoned carcass. In the decomposition stage, the first insect encountered is the order (Diptera) where these insects damage internal organs such as the brain and liver and also damage body cells. The temperature results of local pig carcasses placed outdoors ranged from 32.2-34.50C, while indoor carcass temperatures ranged from 26.77-33.43 0C. The degree of acidity (pH) of carcasses placed outdoors ranges from 5.13-8.53 while indoor carcasses ranges from 6.0-7.77. The environmental temperature of animal bodies placed outdoors ranges from 26.35-39,740C, while humidity ranges from 57.79-86.10%. The temperature of animal bodies in the room ranges from 27.54-31,520C, while humidity ranges from 71.50-85.37%.
Co-Authors Alfonds Andrew Maramis Anice I. Tuda Anita C. C. Tengker Arrijani - Budiarto Buyang, Dalle Carolin Manuahe Decky D. W. Kamagi Dewa Nyoman Oka Dina Munawaroh Dina Rombot Djaila, Friscila Avianti Edieli Zebua Edyson Maneasa Emma M Moko Esibrena Br Kemit Eurika, Uwuh Jessie Fadly Steven Jefry Rumondor Fanny Nella Nanlohy Febiola Gabriela Tular Febiola Najoan Ferencia Esananda Rattu Ferni Margo Tumbel Ferny Margo Tumbel Feydina Tewu Friska Mery Montolalu Futwembun, Fransiska Gedoan, Sukmarayu Piter Grace Jenny Soputan H. M. Sumampouw Hariyadi Hariyadi Helen J Lawalata Helen J Lawalata Helen Joan Lawalata Hendra Pratama Maliangkay Hermanus, Britney Putri Herry Maurits Sumampouw Ilham Saputra Butar-butar Iriani Setyawati IRIANI SETYAWATI Jacklin Stella Manopo Jantje Ngangi Jantje Pelealu Jeane Mantiri Jimmy Langi Jonathan Polandos julduz ruland paus Kamagi, Decky D. W. Kandowangko, Sandy Kaunang, Eva S. N. Maidangkay, Vonny Mamuaya, Tripena Manampiring, Noni Manampiring, Nonny Mansyur, Djailani Masje Wurarah Masje Wurarah Masye Wurarah Max Tulung Meike Paat Meisa Tabita Rogahang Meity Nelltje Tanor Melita Irene Gracia Kaligis Meyer Worang Matey Migau, Natalina Mongdong, Priskila Indah Theresya Motulo, Serlin J Mulder, Keysia Stephanie Tesalonica Mundaeng, Camel Gantonio Nerni Potolangi Nonny Manampiring Nova L.I.M Ogi Ogi, Nova L I M Ogi, Nova L.I.M. Orbanus Naharia Orbanus Naharia Pomalingo, Moh Fikri Pratasik, Stralen Pratasik, Veronika Pua, Devis Christian Rahardiyan, Dino Revolson Mege Rolef Rumondor Rudi Alexander Repi Rymond Jusuf Rumampuk Sibala, Henike Simbolon, Kristina Br Sinyo Herry Sumampouw Suddin Simandjuntak Sukmarayu Gedoan Sukmarayu P. Gedoan Sumampouw, Harry Maurits Taariwuan, Marlin Bernadet Tengker, Anita Costanci Christine Thesia Megi Kurniawati Rettob Timbuleng, Nobel Tjiakra, Rosalina A. Tuegeh, Reinny Silvana Tumanduk, Yahya Y.R. Turambi, James S. Utari Satiman Verawati I Y Roring Verawati Ida Yani Roring Verra E. J. Mawitjere Wisda Nurjannah Abdullah Worang, Rina L. Wuwungan, Axel Yessie K. Kelly