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Kusdiantoro Mohamad
Fakultas Kedokteran Hewan Institut Pertanian Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Journal : ANNALES BOGORIENSES

 1 
Biological Analysis of Leydig Cells-Conditioned Medium To Support Rat Bone Marrow Mesenchymal Stem Cells Differentiation Kaiin, Ekayanti Mulyawati; Prasetyaningtyas, Wahono Esthi; Mohamad, Kusdiantoro; Djuwita, Ita; Yusuf, Tuty Laswardi; Setiadi, Mohamad Agus
ANNALES BOGORIENSES Vol 22, No 1 (2018): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ab.v22i1.328

Abstract

The developed Leydig cells-conditioned medium (LCM) contains bioactive materials secreted by Leydig cells in vitro.  LCM was used to evaluate the ability of bone marrow mesenchymal stem cells differentiation. Bone marrow mesenchymal stem cells (1x 106 cell/ml) were cultured in : 1) DMEM supplemented with 10% NBCS as a control (M), 2) M supplemented with 10 ng/ml testosterone; 3) M supplemented with 50%  LCM ; 4) M supplemented with 50% LCM and 2.5 IU/ml hCG. Bone marrow mesenchymal stem cells that were cultured with  LCM has a positive reaction (57.4%) to histochemistry staining 3β-HSD and produced 1.87 ng/ml testosterone. Supplementation of hCG to LCM  increased the positive number of Leydig cells and testosterone production by 74.6% and 12.33 ng/ml (P<0.05). It can be concluded that Leydig cells-conditioned medium can support differentiation of bone marrow mesenchymal stem cells into Leydig cells.
Biological Analysis of Leydig Cells-Conditioned Medium To Support Rat Bone Marrow Mesenchymal Stem Cells Differentiation Ekayanti Mulyawati Kaiin; Wahono Esthi Prasetyaningtyas; Kusdiantoro Mohamad; Ita Djuwita; Tuty Laswardi Yusuf; Mohamad Agus Setiadi
ANNALES BOGORIENSES Vol 22, No 1 (2018): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (457.602 KB) | DOI: 10.14203/ann.bogor.2018.v22.n1.20-26

Abstract

The developed Leydig cells-conditioned medium (LCM) contains bioactive materials secreted by Leydig cells in vitro.  LCM was used to evaluate the ability of bone marrow mesenchymal stem cells differentiation. Bone marrow mesenchymal stem cells (1x 106 cell/ml) were cultured in : 1) DMEM supplemented with 10% NBCS as a control (M), 2) M supplemented with 10 ng/ml testosterone; 3) M supplemented with 50%  LCM ; 4) M supplemented with 50% LCM and 2.5 IU/ml hCG. Bone marrow mesenchymal stem cells that were cultured with  LCM has a positive reaction (57.4%) to histochemistry staining 3β-HSD and produced 1.87 ng/ml testosterone. Supplementation of hCG to LCM  increased the positive number of Leydig cells and testosterone production by 74.6% and 12.33 ng/ml (P<0.05). It can be concluded that Leydig cells-conditioned medium can support differentiation of bone marrow mesenchymal stem cells into Leydig cells.
Co-Authors . NURBARIAH A.S. Satyaningtijas Aditianingrum, Kurnia Alysia Ali, Ihfan Khaidir Andriyanto Andriyanto Arief Boediono Aryani, Nursela Sofyanti Mirza Bambang Purwantara Damiana Rita Ekastuti Deny Putra Romadhon Edwar, Syafri Ekayanti Mulyawati Kaiin Erdiansyah Rahmi Faisal Mustafa Faralinda Sari Fitri Susana Gatot Prasojo Gustini Syahbirin Hamny Sofyan Hardiansyah, Muhamad Evans HERA MAHESHWARI Husna, Hanifati Iman Supriatna Ita Djuwita ITA DJUWITA ITA DJUWITA Ita Djuwita Ita Djuwita Kaiin, Ekayanti Mulyawati Kaiin, Ekayanti Mulyawati Ketut Adnyane Mudite M Agus Setiadi Mahendra, Arindina Mokhamad Fahrudin Muhammad Jalaluddin, Muhammad Ni Wayan Kurniani Karja Nurhidayat . Pardede, Friselsa Perdhana, Ika Satya R. Iis Arifiantini Rahardi, Nindya Anandaputri Rahmadhani, Elsi Rahmaniyah, Wiwit Ridhani Rahminiwati, Min Raudhatul Jannah Ritonga, Mudhita Zikrullah Sri Wahyuni Srihadi Agungpriyono Sulistyana, Yanti Tanjung, Fitri Aisah Tarigan, Ronald Taufan Hidayat TUTY LASWARDI YUSUF Tuty Laswardi Yusuf Vincentia Maria Vivit Riyacumala Wahono Esthi Prasetyaningtijas Wahono Esthi Prasetyaningtyas Wahono Esthi Prasetyaningtyas Wahono Esthi Prasetyaningtyas Wahono Esthi Prasetyaningtyas Wardhani, Lailia Dwi Kusuma Wasmen Manalu Wiwit Ridhani Rahmaniyah Yohan Rusiyanto Yuhara Sukra