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All Journal Sainteks Pembangunan Pedesaan Khazanah Pendidikan Techno (Jurnal Fakultas Teknik, Universitas Muhammadiyah Purwokerto) Proceeding Seminar LPPM UMP Tahun 2014 Sain Akuatik JRST (Jurnal Riset Sains dan Teknologi) Jurnal Perikanan Universitas Gadjah Mada Aquasains : Jurnal Ilmu Perikanan dan Sumberdaya Perairan KACANEGARA Jurnal Pengabdian pada Masyarakat PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Jurnal Pembelajaran Pemberdayaan Masyarakat (JP2M) Altifani Journal: International Journal of Community Engagement Proceeding Biology Education Conference Proceedings Series on Social Sciences & Humanities Proceedings Series on Physical & Formal Sciences Dulang: Jurnal Pengabdian Kepada Masyarakat Techno Jurnal Manusia dan Lingkungan
Dini Siswani Mulia
Universitas Muhammadiyah Purwokerto
Agriculture, Biological Sciences & Forestry Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Public Health Social Sciences Other

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Non-Specific Immune Potentiating activity of Multivitamins in Catfish (Clarias sp.) Cahyono Purbomartono; Ulfatun Rizkiyah; Dini Siswani Mulia; Arief Husin
Sainteks Vol 18, No 2 (2021): Oktober
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (LPPM)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/sainteks.v18i2.13113

Abstract

Fish farming is well developed because many people like to consume fish. The main problem in cultivation is an attack of a disease. Generally, the disease in fish is MAS (Motile Aeromonas Septicemia) which is caused by Aeromonas hydrophila. Prevention of diseases in fish can be done by increasing immunity through the addition of multivitamins in feed. This study aims to determine the ability of multivitamins against non-specific immune responses in African catfish (C. gariepinus) as well as to know the optimum dose. The study used an experimental method with a completely randomized design using 3 treatments, 1 control, and 4 individual replications. The treatment using a multivitamin through feed with a dose of T1 = 2 g kg-1, T2 = 2.5 g kg-1, T3 = 3 g kg-1 and T0 = control. The parameters observed were phagocytic activity, and differential leukocytes of monocytes and lymphocytes. Data were analyzed using Analysis of variance (ANOVA) at the 95% confidence level. If there is a real difference, then proceed with the Duncan Multiple Range test Test (DMRT) at a 95% confidence level. The results showed that the addition of multivitamins through feed on African catfish had a significant effect (p<0.05) on phagocytic activity and differential leukocyte of monocytes, but had no significant effect (p> 0.05) on differential leucocytes of lymphocyte. The optimum dose for increasing non-specific immune responses in African catfish is T2 at a dose of 2.5 g kg-1 of feed. Based on research, the addition of multivitamins through the feed can potentially be used in fish farming to increase immunity.
Pengobatan Lele Dumbo (Clarias gariepinus L.) yang Terinfeksi Aeromonas hydrophila di Kabupaten Banyumas dengan Menggunakan Ekstrak Daun Api-Api (Avicennia marina) Dini Siswani Mulia; Syiva Vauziyyah
Sainteks Vol 18, No 1 (2021): April
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (LPPM)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/sainteks.v18i1.10650

Abstract

Aeromonas hydrophila merupakan salah satu bakteri patogen yang sering menginfeksi lele dumbo (Clarias gariepinus L.) dan menyebabkan penyakit motile aeromonas septicemia (MAS) atau dikenal dengan aeromoniasis. Penyakit ini dapat menyebabkan gagal panen dan menimbulkan kerugian yang sangat besar pada budidaya ikan tersebut. Tujuan penelitian ini adalah mengkaji pengobatan lele dumbo yang terinfeksi bakteri A.hydrophila di Kabupaten Banyumas dengan menggunakan ekstrak daun api-api (Avicennia marina). Penelitian menggunakan metode eksperimen dengan rancangan acak lengkap (RAL), yang terdiri atas 4 perlakuan dan 4 kali ulangan. Perlakuan berupa konsentrasi pemberian ekstrak A.marina, yaitu P0=tanpa pemberian ekstrak daun A. marina, P1= konsentrasi ekstrak daun A. marina 0,2 g/L, P2= konsentrasi ekstrak daun A. marina 0,3 g/L, dan P3=konsentrasi ekstrak daun A. marina 0,4 g/L. Pengobatan ikan dilakukan secara rendaman. Proses recovery diamati selama 30 hari setelah pengobatan. Parameter utama dalam penelitian adalah perkembangan penyakit, proses recovery, sintasan, pertambahan berat ikan, dan panjang ikan. Parameter pendukung dalam penelitian, yaitu suhu, pH, dan kadar O2 terlarut. Data sintasan dianalisis menggunakan Analisis of Variance (anova) dan dilanjutkan dengan menggunakan uji Duncan Multiple Range Test (DMRT) pada taraf uji 5%. Hasil penelitian menunjukkan lele dumbo yang diobati dengan menggunakan ekstrak daun A. marina mengalami penyembuhan pada hari ke-12, sedangkan kontrol pada hari ke-30. Hasil analisis data sintasan menunjukkan P0 berbeda nyata (P<0,05) dengan P1, P2, dan P3. Konsentrasi ekstrak A. marina yang paling efektif dan efisien pada penelitian ini adalah 0,2 g/L (P1). Kata-kata kunci: Aeromonas hydrophila, Avicennia marina, Banyumas, ekstrak, lele dumbo
Respon Imun Non-Spesifik Ikan Mas (Cyprinus carpio L.) yang Diberi β-Glukan Melalui Diet Pakan Cahyono Purbomartono; Yusuf Aditya; Dini Siswani Mulia; Juli Rochmijati Wuliandari; Arif Husin
Sainteks Vol 17, No 2 (2020): Oktober
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (LPPM)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/sainteks.v17i2.8970

Abstract

Ikan mas (Cyprinus carpio L.) merupakan  ikan air tawar yang  banyak dibudidayakan di Jawa Barat dan memiliki harga jual yang relatif tinggi sehingga banyak dibudidayakan secara intensif. Tingginya padat tebar dan konsumsi pakan pada budidaya intensif menyebabkan penurunan kualitas air akibat timbunan sisa pakan dan  ekskresi ikan, menimbulkan stress sehingga ikan mudah terserang penyakit. Penyakit yang sering menyerang ikan air tawar adalah Motile Aeromonas Septicemia (MAS), disebabkan oleh Aeromonas hydrophila. Untuk mengatasinya, pada umumnya dilakukan pengobatan dengan antibiotik, namun dapat mengakibatkan resistensi bakteri sehingga perlu alternatif lain. Salah satu alternatif adalah penggunaan ß-glucan dari ekstraksi ragi roti Saccaromyces cerevisiae untuk meningkatkan imunitas ikan. Penelitian ini bertujuan untuk mengetahui pengaruh penggunaan β-glucan terhadap respon imun non-spesifik pada ikan mas. Penelitian ini menggunakan metode eksperimen dengan rancangan acak lengkap (RAL) terdiri atas 5 perlakuan dan 3 ulangan. Dosis ß-glucan yang digunakan 0 g, 2,5 g, 5 g, 7,5 dan 10 g per kg pakan. Parameter yang diamati yaitu diferensial leukosit meliputi persentase limfosit, monosit dan neutrofil serta aktivitas fagositosis. Analisis data menggunakan Analisis of Varians (ANOVA) dengan tingkat kepercayaan 95% dilanjutkan dengan uji Duncan Multiple Range Test (DMRT) untuk melihat pengaruh antar perlakuan. Hasil penelitian menunjukkan bahwa penambahan ß-glucan melalui pakan berpengaruh signifikan terhadap persentase limfosit, peresentase neutrofil dan aktivitas fagositosis namun tidak berpengaruh signifikan terhadap persentase monosit. Dosis optimum untuk meningkatkan respon imun non-spesifik ikan mas yaitu 5 g/kg pakan.
ISOLASI, KARAKTERISASI, DAN IDENTIFIKASI BAKTERI PADA LELE DUMBO YANG TERSERANG PENYAKIT DI KABUPATEN BANYUMAS Dini Siswani Mulia; Heri Maryanto; Cahyono Purbomartono
Sainteks Vol 8, No 1 (2011): SAINTEKS
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (LPPM)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/sainteks.v8i1.299

Abstract

The aim of this research is to isolate, characterize, and identify bacteria on infected African catfish in Banyumas Regency. The researcher used survey method and purposive random sampling technique. Samples were infected African catfish. They were taken from three areas that had potential cultivation for African catfish in Banyumas, namely Rempoah, Kebanggan, and Singosari. In each area, the researcher chose two African catfish cultivating ponds randomly and each pond was taken randomly 3 fishes. Symptoms of the disease in African catfish sample were carefully observed, both externally and internally with surgically. External observation covered wounds and bleeding in some parts of the body, sores or ulcers on the body, protruding eyes, while the internal observation included kidney. Isolation of bacteria carried from the skin (scales), body sores, fin, and kidney. Characterization was done by observing the structure of macroscopic, microscopic, and biochemical tests. The results indicated that 130 isolates of bacteria, and identified to be genus Alcaligenes, Bacillus, Arachnia, Acidomonas, Aeromonas, Amphibacillus, Salmonella, Neisseria, Pseudomonas, Micrococcus, and Clostridium. Keywords: isolation, characterize, identify, bacterium, African catfish, Banyumas regency
Respon Imun Non-spesifik Ikan Gurami (Osphronemus gouramy) yang Diberi Fucoidan dari Ekstrak Rumput Laut Cokelat Padina sp. Cahyono Purbomartono; Dini Siswani Mulia; Danang Priyambodo
Sainteks Vol 16, No 1 (2019): April
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (LPPM)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/sainteks.v16i1.7012

Abstract

Fucoidan merupakan senyawa polisakarida sulfat yang diekstrak dari rumput laut cokelat Padina sp. Fucoidan diketahui mempunyai beberapa bioaktivitas, salah satunya sebagai imunostimulan. Ikan gurami (Osphronemus gouramy) ukuran + 100 gr diberi perlakuan fucoidan yang dicampur kedalam pakan dengan dosis 0,2 gr kg-1 pakan (P1), 0,4 gr kg-1 pakan (P2) , 0,6 gr kg-1 pakan (P3) serta kontrol (Po) masing-masing dengan 3 kali ulangan. Hasil penelitian menunjukkan, fucoidan dari ekstrak rumput laut cokelat Padina sp. secara signifikan dapat meningkatkan respon imun non-spesifik terhadap persentase hematokrit, leukokrit dan superoksida anion pada ikan gurami. Perlakuan P3 (0,6 gr kg-1) merupakan dosis optimum yang dapat meningkatkan respon imun non-spesifik ikan, menunjukkan fucoidan dapat digunakan dalam budidaya ikan gurami.Kata kunci: fucoidan, Padina sp., ikan gurami, imun non-spesifik
Kandungan Asam Amino Tepung Bulu Ayam yang Difermentasi dengan Bacillus licheniformis B2560 dan Bacillus subtilis Sebagai Bahan Baku Pakan Ikan Dini Siswani Mulia; Arief Husin; Juli Rochmijati Wuliandari
Sainteks Vol 18, No 2 (2021): Oktober
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (LPPM)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/sainteks.v18i2.13067

Abstract

Kualitas pakan ikan salah satunya ditentukan oleh kandungan protein dan asam amino. Penelitian bertujuan untuk mengkaji kandungan asam amino tepung bulu ayam yang difermentasi dengan Bacillus licheniformis B2560 dan Bacillus subtilis sebagai bahan baku pakan ikan. Penelitian menggunakan metode eksperimen dengan Rancangan Acak Lengkap (RAL) pola faktorial, yaitu perlakuan I : fermentasi (A1 : kontrol/non fermentasi, A2 : fermentasi dengan B. licheniformis B2560, A3 : fermentasi dengan B. subtilis), dan perlakuan II : jumlah inokulum (B1 : jumlah inokulum 5 mL, B2 : jumlah inokulum 10 mL, B3 : jumlah inokulum 15 mL, masing-masing untuk tepung bulu ayam sebanyak 2 g). Parameter penelitian yang diamati adalah kandungan asam amino. Data dianalisis menggunakan Analysis of Variance (Anova) dan dilanjutkan dengan Duncan Multiple Range Test (DMRT) pada taraf uji 5%.  Hasil penelitian menunjukkan bahwa fermentasi tepung bulu ayam menggunakan B. licheniformis B2560 dapat meningkatkan kandungan beberapa jenis asam amino lebih tinggi dibandingkan dengan B. subtilis. Jumlah inokulum 15 mL lebih optimal dalam mendegradasi protein keratin menjadi asam amino. Jenis asam amino dengan kandungan yang sesuai untuk kebutuhan ikan adalah asam glutamat, serin, glisin, treonin, arginin, tirosin, valin, fenilalanin, isoleusin, dan leusin.
PTK (PENELITIAN TINDAKAN KELAS) DENGAN PEMBELAJARAN BERBASIS KEARIFAN LOKAL DAN PENULISAN ARTIKEL ILMIAH DI SD NEGERI KALISUBE, BANYUMAS Dini Siswani Mulia; Suwarno Suwarno
Khazanah Pendidikan Vol IX No 2 Maret 2016
Publisher : Lembaga Publikasi Ilmiah dan Penerbitan (LPIP)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/jkp.v9i2.1062

Abstract

IbM activity aims to increase knowledge about local knowledge, improve skills develop proposals PTK Based Local Wisdom, compiling reports PTK (class action research) based Local Wisdom, and improve the skills of writing scientific articles. The method used in this activity is participatory learning and action (PLA), include: 1). coordination with partners in preparation for the implementation of the program, contact the team of experts, 2). preparation of several training modules to guide the activities, 3). training local wisdom Banyumas 4). technical training prepare proposals PTK Based Local Wisdom, 5). technical training prepare reports PTK Based Local Wisdom, 6). training technique of writing scientific articles, and 7). mentoring programs and evaluation. The result of this activity is the partner has insight into local wisdom Banyumas, and understand that PTK can be done using local wisdom as PTK Banyumas specific needs and character of students. Partners have the knowledge and skills in the preparation of proposals, the implementation of the PTK in the classroom, and the preparation of reports based on local wisdom PTK, partners have the knowledge and skill in preparing scientific articles, the partners have managed to draw up a report PTK and scientific articles have been published in scientific journals of education. Keywords: scientific articles, Banyumas, local knowledge, PTK (Classroom Action Research), SD Negeri Kalisube
PENGHAMBATAN EKSTRAK METANOL DAUN DAN BATANG TUMBUHAN MANGROVERhizophoramucronataTERHADAP PERTUMBUHAN BEBERAPA STRAIN BAKTERI Aeromonas hydrophila Heri Maryanto; Ana Miftahul Janah; Dini Siswani Mulia
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 15, No 1 (2018): Proceeding Biology Education Conference
Publisher : Universitas Sebelas Maret

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Abstract

Aeromonas hydrophila is one of the pathogenic bacteria that attack freshwater fish. These bacteria are also pathogenic in brackish water fish and sea and other aquatic animals. Treatment of this bacterial attack should be pursued by using natural materials that are more effective and environmentally friendly, one of which is Rhizophora mucronata. This study aims to determine the inhibition of methanol extract of leaves and stems of R. mucronata mangrove plant on the growth of several strains of A. hydrophila bacteria. The experiment was conducted using experimental method with Completely Randomized Design (RAL) 3 factor factorial, ie A factor (type of bacterial strain): A1 (GPl-04), A2 (GL-01), A3 (GL-02), A4 (GJ- 01), A5 (GK-01), and A6 (GB-01); B (plant part): B1 (stem) and B2 (leaf); C (concentration of methanol extract R. mucronata): C1 (0% / control), C2 (10%), C3 (20%), and C4 (30%), with 3 replications. The parameters observed were the results of phytochemical screening test by Thin Layer Chromatography (TLC) and bacterial growth inhibition (clear zone). Data of clear zone were analyzed using Analysis of Variance (Anova) and Duncan Multiple Range Test (DMRT) with 5% test level, while the result of phytochemical screening test with TLC was analyzed descriptively qualitative. The results showed that R. mucronata mangrove plants potentially inhibited the growth of A. hydrophila because it contains flavonoids, alkaloids, terpenoids, and tannins. The type of bacterial strain and concentration of R. mucronata methanol extract had significant effect (P0 <0,05) on the inhibitory zone diameter but the plant part and interaction of the three factors, ie strains of bacteria, plant part, and concentration of R. mucronata methanol extract were not significantly different P> 0.05). Plant parts of both stems and leaves have the same potential as a natural bactericidal material. Bacteria A. hydrophila GL-01 strain was most sensitive, whereas GK-01 strain was most resistant to R. mucronata methanol extract. 10% concentration is an effective and efficient concentration in inhibiting the growth of A.hydrophila bacteria.
Uji Lapang Pakan Bervaksin Aeromonas hydrophila pada Lele Dumbo di Daerah Banyumas Dini Siswani Mulia; Irma Tri Susanti; Heri Maryanto; Cahyono Purbomartono
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 12, No 1 (2015): Prosiding Seminar Nasional XII Biologi
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (244.28 KB)

Abstract

The main problem in the cultivation of African catfish is a disease MAS (Motile Aeromonas Septicemia) Aeromonas hydrophila bacteria attack. In addition, other problems that also resulted in the successful cultivation of this fish is the high price of fish feed and the cost of production for the procurement of the feed. Needs to be studied the use of feed raw materials that utilize industrial waste as well as the use of vaccines in controlling disease MAS. This study aims to apply feed made from 3 waste, namely chicken feathers, pulp, and trash fish, then add the vaccine (vaccine feed) and tested in the field. Field test conducted in the village of Bangsa, District of Kebasen, Banyumas. This study uses a completely randomized design (CRD) with 3 treatments and 5 replications. The treatment consisted of P1 = vaccine feed 10 days; P2 = vaccine feed 15 days, and P3 = no vaccine feed (controls). Feed was given as much as 5% of the weight of fish / day. Research using plastic tarp length x width x height = 80 x 60 x 60 cm. African catfish which used a length of 10-13 cm with a weight of 7.3 to 21.1 g. Fish reared for 8 weeks. Parameters measured were immune response such as antibody titer, weight gain and length, specific growth rate (SGR), as well as survival. Parameters measured were supporting water quality parameters include water temperature, pH, and dissolved oxygen. Data were analyzed using Analysis of Variance (ANOVA) and Duncan Multiple Range Test (DMRT test) with a test level of 5%. The results showed that the vaccine could increase the production of antibody titers (P <0.05) compared to controls. The use of artificial feed of chicken feathers, pulp, and trash fish are added the vaccine (vaccine feed) can be applied in the field.Keywords: Aeromonas hydrophila, Banyumas, field test, vaccine feed
FERMENTASI TEPUNG BULU AYAM DENGAN Bacillus licheniformis B2560 UNTUK MENINGKATKAN KUALITAS BAHAN BAKU PAKANIKAN Dini Siswani Mulia; Yesi Nartanti; Heri Maryanto; Cahyono Purbomartono
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 11, No 1 (2014): Prosiding Seminar Nasional XI Biologi
Publisher : Universitas Sebelas Maret

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Abstract

Abstract - The purpose of this study is to examine fermentation of chicken feather meal with Bacillus licheniformis B2560 to improve the quality of fish feed ingredients. The study was conducted using an experimental method to completely randomized design (CRD) 4 treatments, 3 replications, ie P0 = non- fermented ; P1 = 5 ml inoculum fermentation with B. licheniformis B2560 ; P2 = 10 ml inoculum fermentation with B. licheniformis B2560 ; and P3 = 15 ml inoculum fermentation with B. licheniformis B2560, respectively for chicken feather meal as much as 2 g .Parameters measured were the levels of protein and organoleptic parameters that support, in the form of the physical properties of chicken feather meal, include color, texture, and smell. Data were analyzed using a protein content analysis of variance (ANOVA) with a confidence level of 95 %, followed by Duncans Multiple Range Test Test (DMRT) with a confidence level of 95 % , while for the data results of the organoleptic qualitatively analyzed descriptively.The results showed that the P0 treatment was significantly different (P < 0.05) with P1, P2, and P3, and between treatments P1, P2, and P3 respectively were significantly different (P < 0.05). The treatment gives the best results is the treatment that P1 has the highest protein content of 84.08 % with a change in the physical properties of white -yellow (color), soft (texture), and less typical sting (smell). Keywords :Bacillus licheniformis B2560, fermentation, the quality of raw materials, chicken feather meal, Fish feed
Co-Authors . Suwarsito Aman Suyadi Ana Miftahul Janah Ani Khusniah Ardhana Wikaneswari Arief Husin Arif Husin Arif Husin Arif Mahdiana, Arif Berlian Bun’ya Rista Cahyono Purbamartono Cahyono Purbomartono Cahyono Purbomartono Cahyono Purbomartono Cahyono Purbomartono Cintya Windarti Danang Priyambodo Didik Trianto Nugroho, Didik Trianto Dwi Ma’rifatun Khasanah Eddy Sumardi, Eddy Eka Yulyanti Eka Yulyanti, Eka Fatih Wulandari Gayuh Prasetyo Budi Hamzah Syarifuddin, Hamzah Haryanto Haryanto Heri Maryanto Heri Maryanto Heri Maryanto Heri Maryanto Heri Maryanto Heri Maryanto Heri Maryanto Heri Maryanto Hery Maryanto, Hery Hindayati Mustafidah Ikhsan Mujahid Irma Tri Susanti Jejentri, Jejentri Juanita Juanita Juli R. Wuliandari, Juli R. Kartika Silfiana, Kartika Miftakhul Mudah, Miftakhul Mochammad Imron Awalludin Muryanto, Muhammad Nadifah, Itqon Ngaynun Nadiya Nur Apreli Noerhaliza Rachman Asriana Dwi Prihandoko, Arief Rachmansyah Rachmansyah Rakhmawati Utami, Rakhmawati Restu Frida Utami Rini Emawati Risna Tri Yuliningsih Rofiqoh Rofiqoh Saputra, Eqwar Shahiffa Nur Pranannisa Siti Muntasiroh Sri Wahyuningsih Sugeng Priyadi Suwarno Suwarno Syaukaty Yasinta Syiva Vauziyyah Triyanto Triyanto Ulfatun Rizkiyah Wakhudin, Wakhudin Widjarnako, Beny Widya Apriyanti, Widya Wildan Ahid Mujamal Yanuar Verianto, Yanuar Yesi Nartanti Yusuf Aditya