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Dini Siswani Mulia
Universitas Muhammadiyah Purwokerto
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Nursing Public Health Social Sciences Other

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POTENTIAL IMMUNOGENICITY OF BACTERIA Aeromonas hydrophila GPL-05 AND GL-02 STRAINS AS A CANDIDATE VACCINES Dini Siswani Mulia; Ani Khusniah; Heri Maryanto
AQUASAINS Vol 4, No 1 (2015)
Publisher : Jurusan Perikanan dan Kelautan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (338.21 KB)

Abstract

Aeromonas hydrophila is very potential to be used in vaccines and disease control MAS (Motile Aeromonas Septicemia) caused by the bacteria. However, strains of A. hydrophila bacteria which have high immunogenicity limited. Not to mention, in the field often decrease the pathogenicity of the bacteria as vaccine material resulting in a decrease in the immunogenicity of a vaccine made. Therefore, it is necessary to inventory and collecting strains of A. hydrophila bacteria potentially good that vaccination continuity can be maintained. This study used an experimental method with a completely randomized design (CRD), with treatments such as P1: A. hydrophila vaccine strain GPL-05; P2: A. hydrophila vaccine strain GL-02; and P3: control (PBS), with five replicates.This study aims to assess the potential immunogenicity A.hydrophila bacterial strains as vaccine candidates. Test fish used is African catfish was about 2 months with a length of 10-15 cm and width 15-20 g. The main parameters are observed antibody titer and cross-reaction test, while supporting parameters are parameters of water quality include water temperature, pH, dissolved oxygen. Antibody titer data was analyzed by Analysis of Variance (ANOVA) and Duncan's Multiple Range Test (DMRT) at the test level of 5%, while the data and the results of cross-reaction of water quality parameters observed descriptively. The results showed that the bacteria A. hydrophila strain GPL-05 and GL-02 has a high immunogenicity, thus recommended a candidate vaccine.
Uji Fisik Pakan Ikan yang Menggunakan Binder Tepung Gaplek (Physical Test of Fish Feed Using Cassava Flour Binder) Dini Siswani Mulia; Fatih Wulandari; Heri Maryanto
JRST (Jurnal Riset Sains dan Teknologi) Volume 1 No. 1 Maret 2017: JRST
Publisher : Universitas Muhammadiyah Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (228.442 KB)

Abstract

AbstrakPakan ikan dibuat selain memiliki kandungan nutrisi yang sesuai dengan kebutuhan ikan budidaya, juga secara fisik harus kompak dan stabil di dalam air. Kelemahan yang sering terjadi, sebagian besar kandungan nutrisi sudah terpenuhi tetapi pakan mudah tenggelam di dalam air dan cepat terurai sebelum semuanya dimakan ikan. Langkah strategis adalah menambahkan binder (bahan perekat) dalam pembuatan pakan ikan agar bahan pakan tercampur dengan baik, kompak, serta memiliki daya apung yang baik pula. Salah satu bahan yang berpotensi sebagai binder pakan ikan adalah tepung gaplek. Penelitian ini bertujuan untuk mengkaji uji fisik pakan ikan yang menggunakan binder tepung gaplek. Metode penelitian menggunakan metode eksperimen dengan rancangan acak lengkap (RAL) 4 perlakuan dan 4 kali ulangan, yaitu P0 : pakan komersial (kontrol); P1 : pakan dengan binder tepung gaplek 5 %; P2 : pakan dengan binder tepung gaplek 7,5 %; dan P3 : pakan dengan binder tepung gaplek 10%. Bahan baku pakan adalah tepung bulu ayam yang difermentasi dengan Bacillus licheniformis B2560, ampas tahu yang difermentasi dengan Aspergillus niger, dan tepung ikan rucah. Parameter yang diamati adalah uji fisik pakan ikan meliputi daya apung, tingkat kekerasan, tingkat homogenitas, dan kecepatan pecah pakan ikan serta sebagai data pendukung adalah kadar protein dan kadar air pakan ikan. Parameter uji fisik pakan ikan dianalisis dengan menggunakan Analysis of Variance (ANOVA) dan Duncan Multiple Range Test (DMRT) dengan taraf uji 5%, sedangkan data kadar protein dan kadar air dianalisis secara deskriptif. Hasil penelitian menunjukkan bahwa perlakuan pemberian binder tepung gaplek berpengaruh nyata terhadap hasil uji fisik pakan ikan. Perlakuan dengan binder tepung gaplek dengan konsentrasi 10% memiliki kualitas pakan yang paling baik dan mampu menyamai kualitas pakan komersial. Kadar protein yang dihasilkan pakan uji dapat memenuhi kriteria kebutuhan nutrisi pakan yang berkualitas, yaitu berkisar 30-40%.Kata Kunci : binder, pakan ikan, tepung gaplek, uji fisikAbstractFish feed is made in addition to nutritional value that corresponds to the needs of farmed fish, also must physically compact and stable in the water. Weakness is often the case, most of the nutritional content has been fulfilled but the feed easily sink in water and rapidly unfolding before everything is eaten by fish. Strategic step is to add a binder (adhesive) in the manufacture of fish feed in order to feed ingredients well mixed, compact, and has a buoyancy that good anyway. One potential as a binder ingredient of fish feed is dried cassava flour. This study aims to assess the physical test fish feed using cassava flour binder. The research method used experimental method with a completely randomized design (CRD) 4 treatments and 4 replications, namely P0: commercial feed (control); P1: feed with cassava flour binder 5%; P2: feed with cassava flour binder of 7.5%; and P3: feed with 10% cassava flour binder. Feed ingredients are chicken feather flour fermented with Bacillus licheniformis B2560, tofu fermented with Aspergillus niger, and trash fish flour. Parameters measured were physically test fish feed include buoyancy, the level of violence, degree of homogeneity, and broke the speed of fish feed as well as supporting data is the protein content and the moisture content of fish feed. Physical test parameters of fish feed were analyzed using Analysis of Variance (ANOVA) and Duncan Multiple Range Test (DMRT) with a test level of 5%, while data on the nutrient content were analyzed descriptively. The results showed that treatment of cassava flour Award binders significantly affect the results of physical tests of fish feed. Treatment with cassava flour binder with a concentration of 10% has the most excellent feed quality and able to match the quality of commercial feed. The protein content of the resulting feed can meet the test criteria for the nutritional needs of high quality feed, which ranges from 30-40%.Keywords : binder, cassava flour, fish feed, physical tests
Perbandingan Efikasi Vaksin Produk Intra- dan Ekstraseluler Aeromonas Hydrophila untuk Menanggulangi Penyakit Motile Aeromonas Septicemia (Mas) pada Lele Dumbo (Clarias sp.) Dini Siswani Mulia; Cahyono Purbomartono
Jurnal Perikanan Universitas Gadjah Mada Vol 9, No 2 (2007)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.27

Abstract

The aims of this research were to evaluate the eficacy of intra- and extracellular products vaccines of Aeromonas hydrophila to control Motile Aeromonas Septicemia (MAS) in catfish (Clarias sp.). Catfish with 10-13 cm of total length were used for the experiment with three treatments in five replicates. The treatments were vaccinations with (1) A: intracellular product of A. hydrophila; (2) B: extracellular product of A. hydrophila; (3) without vaccination (control). Vaccination was conducted by intramuscular injection of 5 µg protein/fish (each fish was injected with 0.1 ml of vaccine). Booster was conducted one week after vaccination. The challenge test was conducted two weeks after booster. The results showed that the survival rate, Relative Percent Survival (RPS), and antibody titer of A treatment was significantly different (PA. hydrophila (A treatment) reached 85.33 and 82.26%, respectively. The highest antibody titer was 1843.2 which was obtained by the A treatment. The results suggested that intracellular product vaccine of A. hydrophila was more effective than extracellular product vaccine.
Pengaruh Cara Booster terhadap Efikasi Vaksinasi Oral dengan Debris Sel Aeromonas hydrophila pada Lele Dumbo (Clarias sp.) Dini Siswani Mulia; Rachmansyah Rachmansyah; Triyanto Triyanto
Jurnal Perikanan Universitas Gadjah Mada Vol 8, No 1 (2006)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.161

Abstract

The aims of this research were to evaluate the effect of booster on the efficacy of vaccination with cell debris of Aeromonas hydrophila. Catfish (Clarias sp.) (10-13 cm of total length) were used for experiment with 4 treatments, i.e. (1) OS: oral vaccination and injection booster; (2) OO: oral vaccination and oral booster; (3) OR: oral vaccination and immersion booster; (4) without vaccination and booster (control). Booster was conducted one week after vaccination. The challenge test was conducted two weeks after booster. The results showed that the survival rate, Relative Percent Survival (RPS), Mean Time to Death (MTD), and antibody titer of OS treatment was significantly different (P<0.05) with OO, OR, and control. The survival rate and RPS of fish vaccinated by OS treatment reached 100%. The highest antibody titer was 2048 for OS treatment. The result sugested that oral vaccination followed by booster injection was the most effective method of vaccination.
IMUNOGENISITAS HEAT KILLED Aeromonas hydrophila Strain GB-01, GPd-02, dan GPl-05 SEBAGAI KANDIDAT VAKSIN Dini Siswani Mulia; Cintya Windarti; Heri Maryanto
Techno (Jurnal Fakultas Teknik, Universitas Muhammadiyah Purwokerto) Vol 17, No 2 (2016): Techno Volume 17 No 2, Oktober 2016
Publisher : Universitas Muhammadiyah Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/techno.v17i2.1176

Abstract

Abstrak. Vaksinasi Aeromonas hydrophila merupakan langkah konkrit untuk mengendalikan penyakit Motile Aeromonas Septicemia (MAS) yang disebabkan oleh bakteri A.hydrophila. Namun, diperlukan strain bakteri A. hydrophila yang memiliki imunogenisitas tinggi. Pembuatan vaksin dengan metode heat killed merupakan salah satu cara pembuatan vaksin inaktif A. hydrophila selain vaksin inaktif dengan formalin (whole cell). Penelitian bertujuan untuk mengkaji potensi imunogenisitas heat killed A.hydrophila strain GB-01, GPd-02, dan GPl-05 sebagai kandidat vaksin. Penelitian menggunakan metode eksperimen dengan Rancangan Acak Lengkap (RAL), 3 perlakuan (strain GB-01, GPd-02, dan GPl-05). dan 1 kontrol dengan 4 kali ulangan. Vaksin dibuat dengan memanaskan bakteri pada suhu 100ºC selama 2 jam. Ikan uji adalah lele dumbo berumur sekitar 2 bulan dengan ukuran panjang 9-14 cm. Parameter penelitian meliputi titer antibodi, uji reaksi silang, dan parameter kualitas air, yaitu suhu air, pH, dan kadar oksigen terlarut. Data titer antibodi dianalisis dengan Analysis of Variance (Anova) dan Duncan’s Multiple Range Test (DMRT) pada taraf uji 5 %, sedangkan data hasil reaksi silang dan parameter kualitas air diamati secara deskriptif. Hasil penelitian menunjukkan bahwa strain GB-01, GPd-02, dan GPl-05 dapat meningkatkan titer antibodi dibandingkan kontrol dan bereaksi positif pada uji reaksi silang, sehingga disimpulkan memiliki imunogenisitas yang tinggi dan direkomendasikan menjadi kandidat vaksin. Kata Kunci : Aeromonas hydrophila, heat killed, imunogenisitas, kandidat vaksin, strain GB-01, GPd-02, dan GPl-05
UJI LAPANG PAKAN BERVAKSIN Aeromonas hydrophila PADA LELE DUMBO DI DAERAH CILACAP Dini Siswani Mulia; Sri Wahyuningsih; Heri Maryanto; Cahyono Purbomartono
Techno (Jurnal Fakultas Teknik, Universitas Muhammadiyah Purwokerto) Vol 16, No 2 (2015): Techno Volume 16 No 2 Oktober 2015
Publisher : Universitas Muhammadiyah Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/techno.v16i2.55

Abstract

Masalah utama dalam budidaya lele dumbo adalah penyakit, terutama yang disebabkan oleh bakteri Aeromonas hydrophila. Penyakit tersebut dikenal dengan penyakit MAS (Motile Aeromonas Septicemia). Salah satu penanggulangan penyakit MAS adalah dengan vaksinasi. Masalah lain yang sering muncul adalah tingginya biaya pakan sedangkan harga jual ikan cenderung stabil, sehingga pembudidaya ikan cenderung merugi. Perlu strategi untuk membuat pakan sendiri dari bahan-bahan yang masih memiliki kualitas gizi yang baik tetapi harganya murah bahkan memanfaatkan limbah, bahan tersebut mudah diperoleh dan tersedia setiap saat. Penelitian ini bertujuan untuk menguji secara lapang penggunaan pakan bervaksin pada lele dumbo di daerah Cilacap. Uji lapang dilakukan di Desa Purwodadi, Kecamatan Nusawungu, Kabupaten Cilacap. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 3 perlakuan dan 5 kali ulangan. Perlakuan terdiri dari P1 : pemberian pakan bervaksin selama 10 hari; P2 : pemberian pakan bervaksin selama 15 hari; dan P3 : kontrol (non vaksin). Pakan diberikan sebanyak 5% /bb/ekor/hari. Penelitian menggunakan kolam terpal plastik dengan ukuran panjang x lebar x tinggi : 60 x 60 x 80 cm. Lele dumbo yang digunakan berumur 2 bulan, berukuran panjang 12-15 cm dengan berat 16-25 g. Ikan dipelihara selama 8 minggu. Parameter yang diamati adalah respons imun berupa titer antibodi, pertambahan berat dan panjang, serta sintasan ikan. Parameter pendukung yang diamati adalah parameter kualitas air meliputi suhu air, pH, dan oksigen terlarut. Data dianalisis menggunakan Analysis of Variance (Anova) dan Duncan Multiple Range Test (DMRT) dengan taraf uji 5%. Hasil penelitian menunjukkan bahwa pakan bervaksin dapat meningkatkan produksi titer antibodi (P
Antimicrobial Activity of Excoecaria Agallocha Mangrove Extract in Inhibiting the Growth of Aeromonas Hydrophila by in-Vitro Dini Siswani Mulia; Berlian Bun’ya Rista; Aman Suyadi; Ikhsan Mujahid; Cahyono Purbomartono
Proceedings Series on Social Sciences & Humanities Vol. 8 (2023): Proceeding International Seminar 2022 E-Learning Implementation in Malaysia and Indon
Publisher : UM Purwokerto Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/pssh.v8i.602

Abstract

One of the bacterial diseases that often attack freshwater fish is the red-sore disease, also known as aeromoniasis or Motile Aeromonas Septicemia (MAS). It is caused by bacteria from Aeromonas genus, such as Aeromonas hydrophila. A safe alternative treatment for pathogenic bacteria is the use of antibacterial compounds made from natural ingredients. The Excoecaria agallocha mangrove extract is one of potential alternatives that is useful as natural antimicrobials due to its various contents of antibacterial bioactive compounds. The objective of this study was to determine the antimicrobial activity of E. agallocha mangrove extract in inhibiting the growth of A. hydrophila. The research method was experimental with complete randomized design and factorial pattern of 3 factors (type of plant organ, bacterial strain, and concentration of extract), in 48 treatments and 3 replications. The solvents used consisted of methanol (polar) and n-hexane (non-polar). The plant organs used included leaves and stems. The bacterial strains consisted of GPl-04, GB-01, GL-01, GL-02, GJ-01, and GK-01 with concentrations of leaves and stems extracts of 0, 10, 20, and 30%. The parameter measured was the diameter of inhibition zone, which was done by using disc paper (Kirby Bauer method). Non-parametric Kruskall-Wallis Test and qualitative-descriptive data analysis were applied. The methanol extract of E. agallocha leaves in GJ-01 strain at 10 and 20% concentrations had antimicrobial activity with inhibition zone in 2.55 mm and 2.46 mm, respectively. The n-hexane extract of E. agallocha stem at 20 and 30% concentrations had antimicrobial activity with inhibition zones of 1.88 mm in GPl-04 strain and 1.58 mm in GB-01 strain, respectively. Extract of E. agallocha mangrove exhibited potential as a natural antibacterial to prevent aeromoniasis in fish.
The Effectiveness of Dietary Fucoidan Compared to the Combination of Fucoidan with Turmeric on the Growth of African Catfish (Clarias Gariepinus) Cahyono Purbomartono; Rini Emawati; Dini Siswani Mulia; Haryanto Haryanto
Proceedings Series on Social Sciences & Humanities Vol. 8 (2023): Proceeding International Seminar 2022 E-Learning Implementation in Malaysia and Indon
Publisher : UM Purwokerto Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/pssh.v8i.603

Abstract

To meet the needs of fish consumption for the community, African catfish are cultivated intensively. African catfish cultivation is carried out intensively with high stocking density, but some disadvantages inhibit growth. To support growth, it is done by giving a combination of fucoidan supplements with turmeric. Fucoidan and turmeric are known to increase fish growth, but there are no reports of using a combination of fucoidan and turmeric given in feed to increase fish growth. Therefore, this study wants to determine the effect of fucoidan supplementation with turmeric on the growth of African catfish. The method used was a completely randomized design with treatment B: (400 mg fucoidan + 1350 mg turmeric extract) kg-1 feed, C: (800 mg fucoidan + 1200 mg turmeric extract) kg-1 feed, D: (1200 mg fucoidan + 1050 mg of turmeric extract) kg-1 of feed and control (A), each with 4 replications. The parameters observed were weight and length gain, survival growth rate (SGR), feed conversion rate (FCR), and feed efficiency (FE). Data were analysed using analysis of variance (ANOVA) at the 95% confidence level and continued with Duncan's Multiple Range Test (DMRT). The results showed that the combination of fucoidan supplementation with turmeric significantly increased (p <0.05) weight and length growth, SGR, FCR, and FE. The addition of fucoidan supplements with turmeric can be used to cultivate African catfish with an optimal dose (1200 mg fucoidan + 1050 mg turmeric extract) kg-1 feed.
Pertumbuhan Ikan Patin dengan Penambahan Suplemen Tepung Jahe Melalui Pakan pada Sistim Bioflok Cahyono Purbomartono; Shahiffa Nur Pranannisa; Dini Siswani Mulia; Suwarsito Suwarsito
JRST (Jurnal Riset Sains dan Teknologi) Volume 7 No. 1 Maret 2023: JRST
Publisher : Universitas Muhammadiyah Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (803.127 KB) | DOI: 10.30595/jrst.v7i1.16612

Abstract

Masyarakat konsumen menyukai kan patin (Pangasius sp.) karena rasa dan tekstur dagingnya yang enak. Budidaya intensif digalakkan untuk mencukupi kebutuhan dan permintaan konsumen. Budidaya intensif dilakukan dengan padat penebaran tinggi, namun apabila manajemennya kurang baik berpotensi menimbukan sisa pakan dan feses yang mengendap didasar kolam. Pengendapan sisa pakan dan feses didasar kolam dapat membahayakan kualitas air yang berdampak pada perlambatan pertumbuhan. Untuk mengatasi hal ini, dapat dilakukan dengan budidaya sistim bioflok untuk memperbaiki kualitas air disertai penambahan suplemen tepung jahe melalui pakan untuk meningkatkan pertumbuhanya. Tujuan penelitian ini untuk memastikan ada tidaknya dampak positif budidaya sistim bioflok dengan pakan yang mengandung suplemen tepung jahe terhadap pertumbuhan ikan patin. Penelitian dilakukan di Laboratorium basah kolam bioflok Desa Karangsari, Kembaran, Banyumas. Metode eksperimen digunakan dalam penelitian ini dengan Rancangan Acak Lengkap (RAL), 4 perlakuan, 1 kontrol dan 4x replikasi. Perlakuan berupa pemberian tepung jahe dalam pakan dengan sistim bioflok, yaitu P0 = kontrol, P1= pakan + bioflok, P2 = pakan mengandung 5,63 g tepung jahe kg-1 pakan + bioflok, P2 = pakan mengandung 3,75 g tepung jahe kg-1 pakan + bioflok, P3 = pakan mengandung 1,88 g tepung jahe kg-1 pakan + bioflok. Hasil penelitian menunjukkan pemberian pakan yang dicampur tepung jahe dalam pakan dengan sistem bioflok secara signifikan meningkatkan pertambahan berat dan panjang, laju pertumbuhan harian, rasio konversi dan efisiensi pakan dengan dosis optimal 5,63 g tepung jahe kg-1 pakan. Hal ini menunjukkan, penambahan suplemen tepung jahe melalui pakan dalam sistem bioflok dapat digunkan untuk budidaya ikan patin.
PEMANFAATAN LIMBAH BULU AYAM MENJADI BAHAN PAKAN IKAN DENGAN FERMENTASI Bacillus subtilis (Utilization of Waste Chicken Feather to Fish Feed Ingredients Material with Fermentation of Bacillus subtilis) Dini Siswani Mulia; Risna Tri Yuliningsih; Heri Maryanto; Cahyono Purbomartono
Jurnal Manusia dan Lingkungan Vol 23, No 1 (2016): Maret
Publisher : Pusat Studi Lingkungan Hidup Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jml.18773

Abstract

ABSTRAKPenelitian ini bertujuan untuk memanfaatkan limbah bulu ayam menjadi bahan pakan ikan dengan fermentasi Bacillus subtilis. Penelitian menggunakan metode eksperimen dengan Rancangan Acak Lengkap (RAL) 4 perlakuan, 3 kali ulangan, yaitu P0 : tepung bulu ayam non fermentasi; P1 : fermentasi dengan inokulum B. subtilis 5 mL/2 g tepung bulu ayam; P2 : fermentasi dengan inokulum B. subtilis 10 mL/2 g tepung bulu ayam; P3 : fermentasi dengan inokulum B. subtilis 15 mL/2 g tepung bulu ayam. Parameter yang diamati adalah hasil uji proksimat meliputi kadar protein kasar, kadar air, kadar abu, kadar lemak kasar, kadar serat kasar, dan parameter pendukung yaitu uji organoleptik, berupa sifat fisik tepung bulu ayam, meliputi warna, tekstur, dan bau. Data berupa hasil uji proksimat dianalisis menggunakan ANAVA dan Duncan Multiple Range Test (DMRT) dengan taraf uji 5%, sedangkan untuk data hasil organoleptik dianalisis secara deskriptif kualitatif. Hasil penelitian menunjukkan bahwa pemanfaatan limbah bulu ayam menjadi bahan pakan ikan dapat dilakukan dengan fermentasi B. subtilis. Fermentasi tepung bulu ayam menggunakan B. subtillis dapat meningkatkan kualitas bahan baku pakan ikan. Perlakuan P2 (inokulum 10 mL/2 g tepung bulu ayam)adalah perlakuan yang paling efektif karena menghasilkan protein tertinggi yaitu 80,59%, dengan perubahan sifat fisik menjadi putih sampai putih kekuningan (warna), lembut (tekstur), dan khas kurang menyengat (bau). ABSTRACTThis study aims to utilize waste chicken feathers into fish feed ingredients by fermentation of Bacillus subtilis. The research has done by experimental methods with completely randomized design (CRD) 4 treatments, 3 repetitions, ie P0: non-fermented chicken feather meal; P1: fermentation with B. subtilis 5 mL inoculum/2 g chicken feather meal; P2: 10 mL/2 g chicken feather meal; P3: 15 mL/2 g chicken feather meal. Parameters measured were the proximate test results include the levels of crude protein, moisture, ash, crude fat content, fiber content, and organoleptic parameters that support, in the form of physical properties of chicken feather meal, including color, texture, and smell. Proximate test data were analyzed using Analysis of Variance (ANOVA) and Duncan Multiple Range Test (DMRT) with 5% level, while the organoleptic data were analyzed qualitatively descriptively. The results showed that the utilization of waste chicken feathers into fish feed ingredients can be done by fermentation of B. subtilis. Fermentation chicken feather meal using inoculum B. subtillis can improve the quality of fish feed ingredients. Treatment P2 (inoculum 10 mL/2 g chicken feather meal) is the most effective treatment because it produces the highest protein is 80.59%, with changes in physical properties to be white to yellowish white (colour), soft (texture), and less typical sting (smell).
Co-Authors . Suwarsito Akhmad Darmawan Aman Suyadi Ana Miftahul Janah Ani Khusniah Ardhana Wikaneswari Arief Husin Arif Husin Arif Husin Arif Mahdiana, Arif Berlian Bun’ya Rista Cahyono Purbamartono Cahyono Purbomartono Cahyono Purbomartono Cahyono Purbomartono Cahyono Purbomartono Cintya Windarti Danang Priyambodo Didik Trianto Nugroho, Didik Trianto Dwi Ma’rifatun Khasanah Eddy Sumardi, Eddy Eka Yulyanti Eka Yulyanti, Eka Fatih Wulandari Gayuh Prasetyo Budi Hamzah Syarifuddin, Hamzah Haryanto Haryanto Heri Maryanto Heri Maryanto Heri Maryanto Heri Maryanto Heri Maryanto Heri Maryanto Heri Maryanto Heri Maryanto Hery Maryanto, Hery Hindayati Mustafidah Ikhsan Mujahid Irma Tri Susanti Jejentri, Jejentri Juanita Juanita Juli R. Wuliandari, Juli R. Kartika Silfiana, Kartika Miftakhul Mudah, Miftakhul Mochammad Imron Awalludin Muryanto, Muhammad Nadifah, Itqon Ngaynun Nadiya Nur Apreli Noerhaliza Rachman Asriana Dwi Prihandoko, Arief Rachmansyah Rachmansyah Rakhmawati Utami, Rakhmawati Ratna Kartika Wati Restu Frida Utami Rini Emawati Risna Tri Yuliningsih Rofiqoh Rofiqoh Saputra, Eqwar Shahiffa Nur Pranannisa Siti Muntasiroh Sri Wahyuni Sugeng Priyadi Susanto Susanto Susylowati, Dewi Suwarno Suwarno Syamsiar, Syamsiar Syiva Vauziyyah Triyanto Triyanto Ulfatun Rizkiyah Wakhudin, Wakhudin Widjarnako, Beny Widya Apriyanti, Widya Wildan Ahid Mujamal Yanuar Verianto, Yanuar Yasinta, Syaukaty Yesi Nartanti Yusuf Aditya