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All Journal VALENSI Jurnal Penelitian Saintek Jurnal Natur Indonesia Alchemy : Journal of Chemistry Jurnal Ilmu Kimia Universitas Brawijaya Natural B The Journal of Pure and Applied Chemistry Research SEMIRATA 2015 Jurnal Fisika FLUX ALCHEMY Jurnal Penelitian Kimia Indonesian Journal of Chemistry Jurnal Otomasi Kontrol dan Instrumentasi Chemistry Indonesian Journal of Chemical Research Indonesian Journal of Chemical Analysis (IJCA) Molekul: Jurnal Ilmiah Kimia Jurnal Jejaring Matematika dan Sains Bubungan Tinggi: Jurnal Pengabdian Masyarakat Rengganis Jurnal Pengabdian Masyarakat Jurnal Ilmiah Berkala: Sains dan Terapan Kimia Makara Journal of Science
Ani Mulyasuryani
Universitas Brawijaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Social Sciences Other

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Journal : Makara Journal of Science

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Construction and Characterization of Conductometric Biosensor for Determination of the Diazinon Concentration Prayoga, Indrajid; Mulyasuryani, Ani; Prasetyawan, Sasangka
Makara Journal of Science Vol. 8, No. 1
Publisher : UI Scholars Hub

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Abstract

Excessive diazinon residue in vegetables can endanger human health. Therefore, a simple, fast, and accurate method is needed to detect residue. A conductometric biosensor is a good choice because it also offers high selectivity and sensitivity. The principle of detection of the conductometric biosensor is based on enzymatic hydrolysis of diazinon into O,O diethyl phosphorothiate,2-isopropyl-6-methylpyrimidin-4-ol, and H+ catalyzed by organophosphate hydrolase (OPH). The optimum amount of organophosphate hydrolase added to the screen-printed carbon electrode (SPCE) modified with BSA-glutaraldehyde is 118.5 µg, while the optimum pH is 8.5. This biosensor has a response time of 30 sec, a linear dynamic range of 0 to 1 ppm, sensitivity of 42.21 µS/ppm, and limit of detection of 0.19 ppm.
Construction and Characterization of Conductometric Biosensor for Determination of the Diazinon Concentration Prayoga, Indrajid; Mulyasuryani, Ani; Prasetyawan, Sasangka
Makara Journal of Science Vol. 18, No. 1
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Excessive diazinon residue in vegetables can endanger human health. Therefore, a simple, fast, and accurate method is needed to detect residue. A conductometric biosensor is a good choice because it also offers high selectivity and sensitivity. The principle of detection of the conductometric biosensor is based on enzymatic hydrolysis of diazinon into O,O diethyl phosphorothiate,2-isopropyl-6-methylpyrimidin-4-ol, and H+ catalyzed by organophosphate hydrolase (OPH). The optimum amount of organophosphate hydrolase added to the screen-printed carbon electrode (SPCE) modified with BSA-glutaraldehyde is 118.5 µg, while the optimum pH is 8.5. This biosensor has a response time of 30 sec, a linear dynamic range of 0 to 1 ppm, sensitivity of 42.21 µS/ppm, and limit of detection of 0.19 ppm.
Co-Authors - Muhiroh Adam Wiryawan Adam Wiryawan Afifah Muhimmatul Mustaghfiroh Akhmad Sabarudin Akhmad Sabarudin Akhmad Sabarudin Akhmad Sabarudin Akhmad Sabarudin Alfita Safitri Alfita Savitri Anisa Resti Anna Roosdiana Arie Srihardyastutie Arumdati, Rara Aulia Asnaili Alfi Nurillah Attasith Parnsubsakul Ayu Rahayu Anggraeni Azizah, Alfi Bambang Ismuyanto Barlah Rumhayati Barlah Rumhayati Barlah Rumhayati Chandrawati Cahyani Danar Purwonugroho Dewi Umaningrum Dewi, Latifah Tribuana Diah Mardiana Diah Mardiana Diah Mardiana Dian Nur Fajariati Didik Pramana Dwi Oktavianti Eka Krisnaniningrum, Elvian Eka Novitasari Elvian Eka Krisnaniningrum Elvian Eka Krisnaniningrum Enggar Dwi Prasetya, Desta Erasti Pratiwi Erica Marista Rosida Hanandayu Widwiastuti Hasanuddin Hasanuddin Helmi Auliyah Istiqomah Hermin Sulistyarti Ika Diah Safitri Intan Frina Utamiyanti Isvani, Nuzulul Kurniawan Khairunnisa, Alda Khoirul Ngibad Krisnaniningrum, Elvian Eka Layta Dinira Layta Dinira Mardhatillah Mardhatillah Maria Dewi Astuti Moftah Ali Mokhammad W. Dahlan Muhammad Iqbal Fahmi Muhimmatul Mustaghfiroh, Afifah Mustaghfiroh, Afifah Muhimmatul Nanang Tri Widodo Nareswari, Aninda Nashiroh, Ni'matun Nongnoot Wongkaew Nugraha, Hedhi Setya Nuzulul Kurniawan Isvani Oki Mandalia Antasari Okky Anggraito Patsamon Rijiravanich Pazar Ramadani, Dhody Ponco Prananto, Yuniar Prasetya, Desta Enggar Dwi Prayoga, Indrajid Qonitah Fardiyah Qonitah Fardiyah Rachmat Triandi Tjahjanto Radna Nurmasari Rahmani, Indri Alidha Ramadani, Dhody Pazar Rika Endara Safitri Risti, Sandria Gita Rizki Sugiri Roosdiana, Anna Rossy Dwi Adhi Pratiwi Rumhayati, Barlah Sabarudin, Akhmad Safitri, Alfita Sasangka Prasetyawan Sasangka Prasetyawan Sendy Kurniawan Septi Fajar Raeni Setyorini, Dian Ayu Soebiantoro soebiantoro Suryantoro, Angga Sutrisno, Sutrisno Ulfa Andayani Ulfa Andayani Ulfa Andayani, Ulfa Unsania Haresmawati Yuniar Ponco Prananto ZAINUL ABIDIN Zainuri, Akhmad