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All Journal Journal of Tropical Biodiversity and Biotechnology Jurnal Dinamika Pengabdian (JDP) Indonesia Chimica Acta Indonesian Journal of Chemistry Communications in Science and Technology SELAPARANG: Jurnal Pengabdian Masyarakat Berkemajuan Chemistry Indonesian Journal of Chemical Research Jurnal Pengabdian Masyarakat Borneo Abdimas Galuh: Jurnal Pengabdian Kepada Masyarakat Indobiosains Molekul: Jurnal Ilmiah Kimia
Hasnah Natsir
Department of Chemistry, Faculty of Mathematics and Natural Sciences, Hasanuddin University, Jl. Perintis Kemerdekaan Km 10 Tamalanrea, Makassar 90245, South Sulawesi
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Journal : Molekul: Jurnal Ilmiah Kimia

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Isolation and Identification of Cellulolytic Bacteria from Gut of Horn Beetle Larvae (Oryctes rhinoceros L.) Riskawati, Riskawati; Natsir, Hasnah; Dali, Seniwati; Baharuddin, Maswati
Molekul Vol 18 No 2 (2023)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2023.18.2.6848

Abstract

The horn beetle larvae (Oryctes rhinoceros L.) contain symbiotic bacteria that are used to digest and degrade cellulose as food so that it has the potential to produce cellulase enzymes. This study aims to isolate, characterize and identify microbial symbionts from horn beetle larvae that have the potential to produce cellulase enzymes.The methods in this study include morphology and physiology identification of bacteria, qualitative and quantitative activity tests and species determination using 16S rRNA sequencing technique. Based on the results of morphological observations, five bacterial isolates were taken which has the potential as a cellulase producer is indicated by the presence of a clear zone that is produced when a qualitative test is carried out using congo red staining with different cellulolytic indices. Based on the quantitative bacterial activity test using UV-Vis, the highest activity was found in PES3 isolates at 1.62 x 10-2 and PES5 at 1.61 x 10-2. Species determination results found that PES3 isolates belonged to the genus Acinetobacter and PES5 belonged to Pseudomonas. In addition to the isolates obtained for the environment and the industrial sector, cellulolytic bacteria can provide added value such as hydrolyze cellulose waste into alternative fuels.
Chitinase Enzyme-Producing Endophytic Bacterias From the Roots of the Plant Gembolo (Dioscorea bulbifera): Isolation, Characterization and its Potential as an Antifungal Agent Yunita, Vivi Alfi; Natsir, Hasnah; Ahmad, Ahyar; Baharuddin, Maswati
Molekul Vol 19 No 1 (2024)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2024.19.1.9422

Abstract

Chitinase is an enzyme of the chitinolytic group that has many roles in agriculture, especially as an antifungal, because chitin is one of the constituent components of the fungal cell wall. This study aimed to isolate and identify endophytic bacteria from gembolo (Dioscorea bulbifera) roots and to characterize the chitinase enzyme from these endophytic bacteria to be used as an antifungal. Isolation and identification of gembolo plant root endophytic bacteria using PCR method of 16S rRNA gene amplification and sequencing. Characterization of the chitinase enzyme produced includes determining of optimum pH, temperature, and substrate UV-Vis spectrophotometer. Antagonistic test of the chitinase enzyme and endophytic bacterial isolates (isolate K4) Fusarium oxysporum. The results showed that bacterial isolate K4 had with chitinolytic index of 2.45 mm. Electrophoresis results with PCR 16s rRNA gene; the length of the amplified fragment is the position of 1300 bp. By doing the BLAST process in GenBank, the bacterial isolate has 97.93% similarity with Enterobacter cloacae. Then, this endophytic bacteria is called Enterobacter cloacae K4-G. This bacterium produced chitinase enzyme reaching maximum chitinase activity at the 38 hours with an activity of 0.0312 U/mL. The chitinase characterization results of E. cloacae K4-G showed that the optimum conditions were reached at at pH 6, temperature 45 ᵒC, and 2.5% substrate with a chitinase activity value of 0.2467 U/mL. Chitinase enzyme and bacteria Enterobacter cloacae K4-G can inhibit the growth of the fungus Fusarium oxysporum. Therefore, Chitinase from Enterobacter cloacae K4-G can be used as an antifungal pathogen in plants.
Co-Authors A Arfah, Rugaiyah Abdul Karim Abdur Rahman Arif, Abdur Rahman Ahyar Ahmad Alam, Muh. Nur Ali Muhakim Andi Evi Erviani Anggriani, Dhea Faraditha Anita Anita Arfah, Rugaiyah Arfah, Rugaiyah A Arfah, Rugaiyah A. Aziz, Fitria Chatarina Umbul Wahyuni Djabal Nur Basir dwyana, Zarsawaty Fauziah, St Gita, Gita Harningsih Karim Indah Raya Irfandi, Rizal Karimah, Aulia Khairunnur, Siti Mardianti, Riska Mardiyanti, Riska Maswati Baharuddin Muh. Yusri Karim Muhammad Nadir Muhammad Zakir Munandar, Nurharis Musa, Bulkis Nur Asmi Nur Haedar Paulina Taba Permatasari, Nur Umriani Prastawa Budi Ramadani, Ananda Rasyid, Herlina Rauf, Wahyudin Riskawati Riskawati, Riskawati Sarlan, Sarlan Sarni Sarni Sartika Sartika Seniwati Dali SITI HALIMAH LAREKENG, SITI HALIMAH Situmorang, Sarah Victoria Octovianni Solly Aryza St Fauziah, St Fauziah Sukmawaty, Eka Suriati Eka Putri Syaharuddin Kasim Yunita, Vivi Alfi Yusafir Hala